Article
Chemistry, Analytical
Shuai Huang, Haiyang Wang, Xinxin Liu, Lanxiang Liu, Dan Liu, Xiaozhe Zhang, Lihua Zhang, Peng Xie, Yukui Zhang
Summary: This study presents a mass spectrometry imaging (MSI) approach for mapping thiol compounds on brain tissue sections. The developed method allows for selective and rapid detection of glutathione (GSH) synthesis and metabolic pathway related metabolites. The method also enables quantitative detection of exogenous N-acetylcysteine tissue sections, with a detection limit of 0.05 ng. The capabilities of the method are demonstrated through mapping thiol compounds on brain tissue from a chronic social defeat stress (CSDS) depression model mice.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Fan Li, Xiaojing Wu, Hongli Liu, Mengqi Liu, Zhengkai Yue, Zhenyu Wu, Lei Liu, Fuchang Li
Summary: This study reveals that copper depletion enhances ferroptosis by causing mitochondrial perturbation and reducing antioxidative mechanisms.
Article
Biochemistry & Molecular Biology
Talia Ho, Soha Ahmadi, Kagan Kerman
Summary: Alzheimer's disease, a progressive neurodegenerative disorder, affects approximately 40 million people worldwide. Despite extensive research on its pathogenesis and risk factors, treatment options remain limited. The role of copper and glutathione in Alzheimer's disease, as well as their interactions, have been relatively understudied. This review aims to provide a more accessible understanding of their individual roles and interactions in Alzheimer's disease by collecting and assessing relevant research.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Plant Sciences
Lara Vogelsang, Karl-Josef Dietz
Summary: This study investigates the regeneration specificity of thiol-dependent peroxiredoxins and glutathione peroxidase-like enzymes in the cytosol of Arabidopsis thaliana cells. The results demonstrate redundancy and specificity in the redox regulatory network of the cytosol, providing insights into the role of thioredoxins in this process.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Environmental Sciences
Javed Ahmad, Rubina Yasmeen, Mohammad Irfan, Asma A. Al-Huqail, Mohammad Irfan Qureshi
Summary: Traces of arsenic in vegetables and crops cultivated in arsenic-polluted soils are a global concern due to its carcinogenic properties and harmful effects on the food chain. This study focused on the accumulation and defense mechanisms of fenugreek plants exposed to arsenic stress. The findings showed increased oxidative stress and DNA damage at higher arsenic concentrations.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Soodabe Esmaielzadeh, Hormoz Fallah, Yosoof Niknejad, Mehran Mahmoudi, Davood Barari Tari
Summary: This study investigated the effects of methyl jasmonate (MJ) on rice plants under aluminum (Al) stress. The results showed that MJ improved the tolerance of rice to Al stress by maintaining ion homeostasis, enhancing the activity of antioxidant enzymes and the glyoxalase system, and increasing the accumulation of nonprotein thiol compounds.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Giovanna Salbitani, Viviana Maresca, Piergiorgio Cianciullo, Rosanna Bossa, Simona Carfagna, Adriana Basile
Summary: Heavy-metal pollution is a widely discussed problem. It has been shown that heavy metals have biological effects on both animals and plants, including oxidative stress and genotoxicity. Plants, especially metal-tolerant species, have developed various strategies to counteract exposure to toxic metal concentrations. This review discusses the role of non-protein thiol compounds and antioxidant molecules in bryophytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Federico Maria Rubino
Summary: Glutathionyl hemoglobin is a minor form of hemoglobin that plays a crucial role in the response of erythrocytes to oxidative stress. By measuring the redox potential of its active site, insights into the changes in erythrocytes during oxidative and recovery phases can be gained. Research indicates that hemoglobin acts as an important redox buffer in erythrocytes.
Review
Biochemistry & Molecular Biology
Carmen Beatrice Dogaru, Corina Muscurel, Carmen Duta, Irina Stoian
Summary: This review discusses the identity and functions of several less-known selenoproteins, which play important roles in various physiological and pathogenic processes, including antioxidant defense, anti-inflammation, anti-apoptosis, and immune and inflammatory response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Osiris German Idelfonso-Garcia, Brisa Rodope Alarcon-Sanchez, Veronica Rocio Vasquez-Garzon, Rafael Baltierrez-Hoyos, Saul Villa-Trevino, Pablo Muriel, Hector Serrano, Julio Isael Perez-Carreon, Jaime Arellanes-Robledo
Summary: Nucleoredoxin (NXN), an oxidoreductase enzyme, regulates multiple signaling pathways and plays a role in maintaining cellular redox homeostasis. It is involved in various cellular processes and may be associated with different diseases.
Article
Medicine, Research & Experimental
Kuiyu Chen, Limin Wu, Qianzhuo Liu, Fang Tan, Luyao Wang, Dan Zhao, Xinru Fang, Xiang Liu, Jiabo Liu, Hui Han
Summary: This study observed the changes in spermatogenesis, mitochondrial damage, apoptosis, and oxidative stress levels in the testes of Wilson disease mice, and investigated the effect and mechanism of glutathione on testicular spermatogenesis. The results showed that Wilson disease mice exhibited impaired testicular tissue structure, reduced sperm count, increased apoptosis, elevated oxidative stress levels, and abnormal mitochondrial ultrastructure. Glutathione treatment significantly improved testicular tissue structure, sperm count, apoptosis rate, mitochondrial structure, and oxidative stress levels in Wilson disease mice.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Chemistry, Analytical
Ying Zhou, Xiaoyu Shi, Liang Ma, Yaqin Chai, Ruo Yuan
Summary: In this study, an electrochemiluminescence (ECL) biosensor is developed for simultaneous and ultrasensitive detection of glutathione (GSH) and copper ion (Cu2+), using thiol stabilized gold nanoclusters (Au NCs) as a highly efficient ECL emitter. The study reveals that thiol-Au NCs exhibit exceptional ECL performance over amino-Au NCs due to fast electron transfer. The ECL biosensor achieves trace detection of GSH with a detection limit of 148 pM and Cu2+ with a detection limit of 91.5 fM, providing a definite direction for obtaining ultra-bright ECL metal NCs for multiple biomarker testing and expanding their application in clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Filip Cvetko, Stuart T. Caldwell, Maureen Higgins, Takafumi Suzuki, Masayuki Yamamoto, Hiran A. Prag, Richard C. Hartley, Albena T. Dinkova-Kostova, Michael P. Murphy
Summary: The study reveals that the production of mitochondrial superoxide and hydrogen peroxide does not activate the Nrf2 system, while selective disruption of the mitochondrial GSH pool and thioredoxin system leads to Nrf2 activation. These findings provide insights into how disruption to mitochondrial redox homeostasis is sensed and signaled to the nucleus in cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Shuichi Shibuya, Kenji Watanabe, Yusuke Ozawa, Takahiko Shimizu
Summary: While XOR-mediated O-2(center dot-) production contributes to aging-like pathologies in mice, its involvement in age-related tissue damage induced by SOD1 loss remains unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Annamaria D'Alessandro, Giovina Di Felice, Melania Manco, Anna Pastore, Simona Pezzi, Michela Mariani, Danilo Fintini, Andrea Onetti Muda, Ottavia Porzio
Summary: Obesity is a serious public health challenge that is closely linked to oxidative stress and metabolic disorders. This study analyzed serum samples from children with obesity and found abnormal levels of oxidative stress markers, which were positively correlated with obesity-related indices. Age was also found to be associated with oxidative stress.
Article
Biochemistry & Molecular Biology
Tessa R. Young, Tara L. Pukala, Roberto Cappai, Anthony G. Wedd, Zhiguang Xiao
Article
Biochemistry & Molecular Biology
Tessa R. Young, Anthony G. Wedd, Zhiguang Xiao
Article
Chemistry, Multidisciplinary
Ashwinie A. Ukuwela, Ashley I. Bush, Anthony G. Wedd, Zhiguang Xiao
Article
Biochemistry & Molecular Biology
Shashank Masaldan, Shamel A. S. Clatworthy, Cristina Gamell, Zoe M. Smith, Paul S. Francis, Delphine Denoyer, Peter M. Meggyesy, Sharon La Fontaine, Michael A. Cater
Article
Plant Sciences
Gilles Lekeux, Clementine Laurent, Marine Joris, Alice Jadoul, Dan Jiang, Bernard Bosman, Monique Carnol, Patrick Motte, Zhiguang Xiao, Moreno Galleni, Marc Hanikenne
JOURNAL OF EXPERIMENTAL BOTANY
(2018)
Review
Biochemistry & Molecular Biology
Zhiguang Xiao, Sharon La Fontaine, Ashley I. Bush, Anthony G. Wedd
JOURNAL OF MOLECULAR BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Saumya R. Udagedara, Chathuri J. K. Wijekoon, Zhiguang Xiao, Anthony G. Wedd, Megan J. Maher
JOURNAL OF INORGANIC BIOCHEMISTRY
(2019)
Article
Biochemical Research Methods
Shadi Maghool, Sharon La Fontaine, Megan J. Maher
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Pramod Subedi, Jason J. Paxman, Geqing Wang, Ashwinie A. Ukuwela, Zhiguang Xiao, Begona Heras
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Cell Biology
Gonzalo Perez-Siles, Anthony Cutrupi, Melina Ellis, Jakob Kuriakose, Sharon La Fontaine, Di Mao, Motonari Uesugi, Reinaldo Takata, Carlos E. Speck-Martins, Garth Nicholson, Marina L. Kennerson
DISEASE MODELS & MECHANISMS
(2020)
Article
Plant Sciences
Stanislaus Antony Ceasar, Gilles Lekeux, Patrick Motte, Zhiguang Xiao, Moreno Galleni, Marc Hanikenne
FRONTIERS IN PLANT SCIENCE
(2020)
Review
Biochemistry & Molecular Biology
Tessa R. Young, Zhiguang Xiao
Summary: Metal ions play critical roles in biology, and the metal-protein affinity, described by the metal dissociation constant K-D, is a key parameter for understanding metal-protein interactions. This study outlines fundamental principles and practical considerations for reliable quantification of metal-protein affinities, using spectroscopic probes to determine accurate K-D values for essential biological transition metals.
BIOCHEMICAL JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Bryce Blades, Scott Ayton, Ya Hui Hung, Ashley Bush, Sharon La Fontaine
Summary: The complex and inverse relationship between copper and lipid metabolism has implications for diseases involving dyslipidemia. Understanding these pathways may provide druggable targets for therapeutic intervention. Tissue-specific roles of the copper regulatory protein ATP7B further elucidate the association between copper and lipid metabolism.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Ashwinie A. Ukuwela, Ashley I. Bush, Anthony G. Wedd, Zhiguang Xiao
BIOCHEMICAL JOURNAL
(2017)