Article
Oncology
Shiqi Xiang, Zixuan Lin, Meagan J. J. Makarcyzk, Kanyakorn Riewruja, Yiqian Zhang, Xiurui Zhang, Zhong Li, Karen L. Clark, Eileen Li, Silvia Liu, Tingjun Hao, Madalyn R. R. Fritch, Peter G. G. Alexander, Hang Lin
Summary: This study elucidates the difference between hiMPCs and hMSCs in response to TGF beta and presents a robust method for obtaining low hypertrophy hiMPCs-derived cartilage. The results also indicate the importance of activating both Smad2/3 and Smad1/5 in the initiation of chondrogenesis.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Jing Zheng, Yan Xie, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: This study investigated different microenvironmental conditions for the controlled proliferation and chondrogenic differentiation of human bone-marrow-derived mesenchymal stem cells (hMSCs). The results showed that collagen sponges promoted cell proliferation and chondrogenic differentiation. Combining collagen sponges with collagen hydrogels and induction factors had the highest promotive effect on chondrogenic differentiation. These findings provide useful information for stem cell chondrogenic differentiation and cartilage tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Orthopedics
Junfeng Wang, Keshi Zhang, Shaolong Zhang, Zhenpeng Guan
Summary: This study investigates the role of Vaspin on the chondrogenic differentiation of BMSCs and chondrocytes survival, and its involvement of Akt activation. Vaspin promotes the chondrogenic differentiation of BMSCs and chondrocyte survival by activating the Akt pathway. It also increases the expression of markers in cartilage formation and extracellular matrix secretion. These findings provide new insights into the potential ability of Vaspin to ameliorate the chondrogenic differentiation of BMSCs and chondrocyte survival in osteoarthritis (OA).
BMC MUSCULOSKELETAL DISORDERS
(2022)
Article
Nanoscience & Nanotechnology
Yu Zhu, Yi Sun, Biyu Rui, Junqing Lin, Junjie Shen, Huimin Xiao, Xuanzhe Liu, Yimin Chai, Jia Xu, Yunlong Yang
Summary: This study developed a granular hydrogel (GH) for cartilage regeneration that overcomes the limitations of widely used elastic hydrogels. The GH, composed of hyaluronic acid, polyethylene glycol, and gelatin, showed excellent potential in promoting volume expansion, morphology recovery, and chondrogenic phenotype improvement of encapsulated chondrocytes. The GH also stimulated hyaline cartilage matrix deposition and connection, leading to hyaline-like cartilage regeneration in in vivo models. The study suggests the feasibility of GHs as in situ chondrocyte deploying scaffolds and provides new insights for designing hydrogel scaffolds for cartilage tissue engineering.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Orthopedics
Hamza A. Owida, Nicola L. Kuiper, Ying Yang
Summary: Both hyaluronic acid and agarose hydrogels preserved the pericellular matrix of chondrons over a 21-day culture period, with type VI collagen appearing dense and homogeneous. The presence of mesenchymal stem cells did not influence this outcome. Chondrogenic media enhanced the production of key extracellular matrix components, but did not enhance type VI collagen synthesis, suggesting that a 3D chondrogenic hydrogel environment is crucial for pericellular matrix formation.
Article
Engineering, Biomedical
Sepideh Saghati, Reza Rahbarghazi, Ali Baradar Khoshfetrat, Keyvan Moharamzadeh, Hamid Tayefi Nasrabadi, Leila Roshangar
Summary: The study investigated the chondrogenic capacity of phenolated alginate-collagen hydrogel on human amniotic mesenchymal stem cells, showing promising potential for promoting chondrocyte generation and exhibiting certain advantages in terms of physicochemical properties.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Caroline Melou, Pascal Pellen-Mussi, Solen Novello, Damien Brezulier, Agnes Novella, Sylvie Tricot, Pascale Bellaud, Dominique Chauvel-Lebret
Summary: The objective of this study was to develop a three-dimensional culture model for rapidly evaluating cartilage tissue engineering protocols. The spheroid model showed greater fluctuations in chondrogenesis markers compared to the gold standard pellet culture. Additionally, different cell lines from the same organ exhibited distinct biological responses.
Article
Biotechnology & Applied Microbiology
Xiang Li, Shiao Li, Jiatian Qian, Yancheng Chen, Yiqin Zhou, Peiliang Fu
Summary: The study demonstrated that type I collagen-based MACT is an effective treatment for improving functionality and pain levels. Histological evidence confirmed hyaline cartilage induction and showed that repaired cartilage tended to emerge from the deep to the superficial layer. The quantitative MRI T2 mapping test indicated that there still was a difference between the transplanted cartilage and the surrounding hyaline cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mohammed Zayed, Steve Adair, Madhu Dhar
Summary: The study showed that synovial fluid can maintain the proliferation and viability of MSCs and significantly affect chondrocyte differentiation. Stimulation of MSCs with synovial fluid or IFN-gamma can enhance chondrogenesis and anti-inflammatory activity. This suggests that the joint environment is suitable for chondrogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Yong Kwan Noh, Sung Won Kim, Ik-Hwan Kim, Kwideok Park
Summary: The study demonstrated that cells cultured on the natural form of decellularized ECM, N-CHDM, exhibited better proliferation and differentiation properties, with higher levels of chondrogenic marker expression and immunofluorescence of COL II. Moreover, NSCs preconditioned on N-CHDM showed higher cell retention rates, more cells with chondrocyte lacunae structure, and increased production of cartilage ECM when transplanted in nude mice.
BIOMATERIALS RESEARCH
(2021)
Article
Engineering, Biomedical
Xiaoying Jin, Yangyang Dai, Liaobing Xin, Zi Ye, Jiayu Chen, Qianhong He, Xin Chen, Xiaodong Xu, Guanghui Song, Xiaohua Yu, Songying Zhang
Summary: Endometrial injury can lead to infertility, and traditional treatments are limited in their effectiveness. This study presents a novel approach using ECM@ADSC-exos to promote endometrium regeneration. In vitro experiments showed improved cell proliferation, migration, and angiogenesis. In a rat model, ECM@ADSC-exos enhanced endometrium regeneration, promoted myometrium repair, and preserved fertility.
MATERIALS TODAY BIO
(2023)
Article
Cell & Tissue Engineering
Akihiro Yamashita, Hiroyuki Yoshitomi, Shunsuke Kihara, Junya Toguchida, Noriyuki Tsumaki
Summary: This study demonstrated that hiPSCs could form cartilage on Matrigel but not on laminin 511-E8, with differences observed in cell morphology and YAP activity. Transient knockdown of YAP around the start of chondrogenic differentiation successfully addressed the issue of cartilage formation from hiPSCs on laminin 511-E8.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Hideaki Kojima, Hiroko Kushige, Hiroshi Yagi, Takayuki Nishijima, Nobuko Moritoki, Narihito Nagoshi, Yutaka Nakano, Masayuki Tanaka, Shutaro Hori, Yasushi Hasegawa, Yuta Abe, Minoru Kitago, Masaya Nakamura, Yuko Kitagawa
Summary: Cell transplantation using mesenchymal stem cells (MSCs) has shown promising results for repairing and regenerating injured organs. However, the survival and retention of MSCs remain a challenge. This study investigated the co-transplantation of MSCs with decellularized extracellular matrix (dECM) hydrogels and found that it improved the survival rate of engrafted cells and demonstrated therapeutic effects in a rat pancreatitis model.
CELL TRANSPLANTATION
(2023)
Article
Multidisciplinary Sciences
Anggraini Barlian, Dinda Hani'ah Arum Saputri, Adriel Hernando, Candrani Khoirinaya, Ekavianty Prajatelistia, Hutomo Tanoto
Summary: This study utilized a spider silk protein micropattern to direct the chondrogenesis of human Wharton's jelly MSCs. The results demonstrated that the spidroin micropattern enhanced the expression of chondrogenic markers and maintained long-term upregulation of SOX9, indicating its potential as a novel approach for cartilage tissue engineering.
SCIENTIFIC REPORTS
(2022)
Review
Materials Science, Biomaterials
Matthew Walker, Jiajun Luo, Eonan William Pringle, Marco Cantini
Summary: The extracellular matrix is a complex microenvironment that regulates cell fate, and hydrogels, made of synthetic or natural materials, can mimic these matrices to target cell behavior for tissue engineering. By tuning their properties, native tissue frameworks can be recapitulated. This review explores how hydrogels have been engineered to repair damaged cartilage tissue by harnessing stem cell chondrogenic response, discussing the signaling processes involved in hydrogel-driven chondrogenesis.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Orthopedics
Eberhard von Rottkay, Lars Rackwitz, Maximilian Rudert, Ulrich Noeth, Johannes Christian Reichert
INTERNATIONAL ORTHOPAEDICS
(2018)
Article
Cell & Tissue Engineering
V. M. C. Quent, A. V. Taubenberger, J. C. Reichert, L. C. Martine, J. A. Clements, D. W. Hutmacher, D. Loessner
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2018)
Article
Orthopedics
Johannes C. Reichert, Eberhard von Rottkay, Franz Roth, Tim Renz, Johannes Hausmann, Julius Kranz, Lars Rackwitz, Ulrich Noeth, Maximilian Rudert
BMC MUSCULOSKELETAL DISORDERS
(2018)
Article
Orthopedics
L. Rackwitz, J. C. Reichert, M. Haversath, U. Noeth, M. Jaeger
Article
Orthopedics
Josephine Berger-Groch, Darius M. Thiesen, Lars G. Grossterlinden, Jan Schaewel, Florian Fensky, Maximilian J. Hartel
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY
(2019)
Article
Orthopedics
U. Noeth, T. Geiser, T. Kranich, E. von Rottkay, J. C. Reichert, M. Reyle-Hahn, L. Rackwitz
Article
Emergency Medicine
Florian Fensky, Lukas Weiser, Kay Sellenschloh, Matthias Vollmer, Maximilian Johannes Hartel, Michael Maria Morlock, Klaus Puschel, Johannes Maria Rueger, Wolfgang Lehmann
Summary: A novel testing device was developed for biomechanical analyses of the pelvis with intact peripelvic soft tissues. The study found that isolated unilateral fractures of the anterior pelvic ring showed no relevant biomechanical variation compared to the intact situation under isolated axial load.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2021)
Article
Multidisciplinary Sciences
Dimitris Ntalos, Kay Sellenschloh, Gerd Huber, Daniel Briem, Klaus Puschel, Michael M. Morlock, Karl-Heinz Frosch, Florian Fensky, Till Orla Klatte
Article
Biochemical Research Methods
David S. Sparks, Siamak Saifzadeh, Flavia Medeiros Savi, Constantin E. Dlaska, Arne Berner, Jan Henkel, Johannes C. Reichert, Martin Wullschleger, Jiongyu Ren, Amaia Cipitria, Jacqui A. McGovern, Roland Steck, Michael Wagels, Maria Ann Woodruff, Michael A. Schuetz, Dietmar W. Hutmacher
Article
Emergency Medicine
Markus Rossmann, Florian Fensky, Ann-Kathrin Ozga, Johannes M. Rueger, Sven Maerdian, Gabriele Russow, Ulf Brunnemer, Gerhard Schmidmaier, Alexander Hofmann, Philipp Herlyn, Thomas Mittlmeier, Ahmed Amer, Thomas Goesling, Lars G. Grossterlinden
Summary: The purpose of this study was to investigate the potential of classification systems to choose the appropriate operative approach for tibial plateau fractures. The study found that the use of the dorsal approach was less important than expected, and the choice of surgical approach relied more on the surgeons' experience and preferences.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2022)
Article
Orthopedics
Maximilian J. Hartel, Tareq Naji, Florian Fensky, Frank O. Henes, Darius M. Thiesen, Wolfgang Lehmann, Karl-Heinz Frosch, Dimitris Ntalos
Summary: The study investigated the use of an anatomical-preshaped three-dimensional suprapectineal plate for different types of acetabular fractures. Results showed that this method provided good radiological outcomes in a predominantly geriatric cohort, but impaired bone density significantly increased the risk of poor reduction results.
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY
(2022)
Article
Medicine, General & Internal
Johannes Christian Reichert, Andre Hofer, Georg Matziolis, Georgi Iwan Wassilew
Summary: The study aimed to compare the accuracy and reliability of intraoperative fluoroscopic images with postoperative radiographs in evaluating acetabular coverage during periacetabular osteotomies (PAOs). The results showed a high level of consistency and reliability between intraoperative fluoroscopy and postoperative radiographs, indicating that intraoperative fluoroscopy can be used as an alternative imaging modality for assessing acetabular fragment orientation during PAO. The lateral center edge angle (LCEA) and the Tonnis angle (TA) were identified as precise measures for evaluating lateral head coverage, while the anterior and posterior wall index (AWI, PWI) were suitable for assessing acetabular version.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Frank Schulze, Annemarie Lang, Janosch Schoon, Georgi I. I. Wassilew, Johannes Reichert
Summary: Bone has a high ability to regenerate, but large defects sometimes cannot heal. Tissue engineering and regenerative medical strategies have been researched to address this challenge. This review evaluates current approaches and discusses future advanced techniques for bone tissue engineering and their translation to clinical applications.
Article
Biochemistry & Molecular Biology
Andreas Nitsch, Sara Qarqash, Sarah Roemer, Janosch Schoon, Axel Ekkernkamp, Maya Niethard, Johannes C. Reichert, Georgi I. Wassilew, Mladen V. Tzvetkov, Lyubomir Haralambiev
Summary: This study investigated the synergistic effects of cold physical plasma (CPP) and commonly used cytostatic chemotherapeutics on Ewing's sarcoma (ES) cells. The combination of CPP and chemotherapy drugs resulted in significant growth inhibition and increased apoptosis rates in ES cells. These findings suggest that CPP could enhance the efficacy of cytostatic chemotherapeutics, supporting its potential as an anti-tumor therapy in clinical practice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Orthopedics
Manuela Hoffmann, Johannes C. Reichert, Anastasia Rakow, Janosch Schoon, Georgi I. Wassilew
Summary: In 2020, more than 14,000 aseptic revision procedures for total hip arthroplasty (THA) were recorded in Germany. Patients' expectations for revision surgery are similar to primary hip replacement. However, revision surgery has higher complication rates and more dissatisfied patients compared to primary THA, with poorer postoperative function, increased pain levels and impaired quality of life and return-to-work.
