Article
Engineering, Biomedical
Mechiel Nieuwoudt, Ian Woods, Kian F. Eichholz, Carolina Martins, Kate McSweeney, Nian Shen, David A. Hoey
Summary: Functionalized synthetic scaffolds can enhance bone regeneration properties by promoting osteogenic differentiation and matrix mineralization through the attachment of osteocyte-derived EVs. This matrix-based adhesion of EVs provides a mechanism for signal transmission in polyester scaffolds, demonstrating the potential of osteocyte-derived EVs to enhance bone tissue regeneration rate.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Engineering, Biomedical
Yubo Shi, Lei Wang, Liguo Sun, Zhennan Qiu, Xiaoli Qu, Jingyi Dang, Zhao Zhang, Jiankang He, Hongbin Fan
Summary: Melt electrospinning writing (MEW) is a promising 3D printing technology for scaffold fabrication. This study investigated the use of NaOH treatment to enhance the cytocompatibility and osteoinductive properties of polycaprolactone (PCL) scaffolds. The NaOH-treated PCL scaffold exhibited nanopits and nanogrooves on the surface, which increased surface roughness and wettability, leading to improved cell proliferation and adhesion. In vitro experiments demonstrated that the NaOH-treated surface could induce osteogenic differentiation of bone marrow mesenchymal stem cells via the integrin alpha 2/beta 1-PI3K-Akt signaling pathway. Animal studies showed that the NaOH-treated PCL scaffold promoted new bone formation. The study concluded that NaOH treatment is a simple and effective method to enhance the cellular affinity and osteoinductive property of MEW PCL scaffolds.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Biochemistry & Molecular Biology
Iman Shirzaei Sani, Mostafa Rezaei, Ali Baradar Khoshfetrat, Donya Razzaghi
Summary: Chitosan and poly (epsilon-caprolactone) were combined to fabricate bone tissue engineering scaffolds with enhanced bioactivity and mechanical properties. The incorporation of chitosan-g-poly (epsilon-caprolactone) significantly decreased fiber diameter and hydroxyapatite nanoparticles enhanced mineralization and degradation of the scaffold. In vitro cytocompatibility assays showed high cell viability and proliferation, indicating the potential use of the microfabricated nanocomposite scaffolds in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Nanoscience & Nanotechnology
Kateryna Diedkova, Alexander D. Pogrebnjak, Sergiy Kyrylenko, Kateryna Smyrnova, Vladimir V. Buranich, Pawel Horodek, Pawel Zukowski, Tomasz N. Koltunowicz, Piotr Galaszkiewicz, Kristina Makashina, Vitaly Bondariev, Martin Sahul, Maria Caplovicova, Yevheniia Husak, Wojciech Simka, Viktoriia Korniienko, Agnieszka Stolarczyk, Agata Blacha-Grzechnik, Vitalii Balitskyi, Veronika Zahorodna, Ivan Baginskiy, Una Riekstina, Oleksiy Gogotsi, Yury Gogotsi, Maksym Pogorielov
Summary: New conductive materials are needed for tissue engineering to develop regenerative strategies for nervous, muscular, and heart tissues. Polycaprolactone (PCL) is used to create biocompatible and biodegradable nanofiber scaffolds, while MXenes can make these scaffolds conductive and hydrophilic. This study investigates the physical properties, defect structure, and porosity of PCL-MXene composite scaffolds, and demonstrates their advantages over existing conductive scaffolds for tissue engineering in terms of structure, chemistry, electricity, and biology.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Kamath S. Manjunath, K. Sridhar, V Gopinath, KrishnaKumar Sankar, A. Sundaram, Nilkantha Gupta, Ahmed S. S. J. Shiek, Patil S. Shantanu
Summary: The study fabricated a PLA-PCL scaffold combining structural and functional components using FDM, which improved mechanical stability compared to a standalone PLA scaffold. The scaffold exhibited interconnected porous structures and increased crystallinity without chemical alterations during the embedding process. In vitro analysis showed the scaffold's osteoconductive nature, supporting mineralization.
BIOMEDICAL MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Mahnaz Sadat Mirbagheri, Sahar Akhavan-Mahdavi, Anwarul Hasan, Mohammad Saeed Kharazmi, Seid Mahdi Jafari
Summary: Propolis-loaded electrospun nanofibers (PENs) with optimum levels of propolis and two polymer types (PCL and PVA) were developed using response surface methodology (RSM). The scaffold characteristics such as porosity, average diameter, wettability, release, and tensile strength were investigated. The overall optimum region was found to be at PCL/6% PRP and PVA/5% PRP. The cytotoxicity assay showed no toxicity for the optimal concentrations of PRP. Fourier transform infrared (FTIR) spectra revealed that no new chemical functional groups were introduced in the PENs. Uniform fibers were found in the optimum samples without the appearance of a bead-like structure in the fibers. In conclusion, nanofibers containing the optimal concentration of PRP with suitable properties can be used in biomedical and tissue engineering.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Materials Science, Multidisciplinary
Parisa Haji Mohammadi Gohari, Masoumeh Haghbin Nazarpak, Mehran Solati-Hashjin
Summary: The study explored the fabrication and properties of electrospun PCL/rGO scaffolds for bone tissue engineering. The addition of rGO altered the mechanical properties in dry conditions while decreasing all mechanical values in wet conditions. In terms of bioactivity, apatite deposition on PCL/rGO scaffolds was shown to require time, and the degradation rate of PCL was accelerated in scaffolds enriched with rGO. Biological evaluations with MG-63 cell lines suggested a critical correlation between rGO dosage in PCL scaffolds and cell behavior for bone tissue applications.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Nanoscience & Nanotechnology
Xuanwen Xu, Yi Zhou, Kai Zheng, Xinyu Li, Lu Li, Yan Xu
Summary: Periodontitis is a chronic inflammatory disease that affects people worldwide and has an uncertain prognosis with surgical treatment. In this study, a three-dimensional scaffold made of electrospun polycaprolactone/gelatin fibrous membranes was developed to overcome the challenges of periodontal treatment. The scaffold showed good properties and promoted adhesion and proliferation of human periodontal ligament stem cells. In vivo experiments confirmed that the scaffold could guide cellular orientation and potentially facilitate periodontal regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
Elahe Gholipour Choubar, Mohammad Hossein Nasirtabrizi, Farshid Salimi, Ali Sadeghianmaryan
Summary: Electrospun PCL/COL: PVP scaffolds loaded with Cephalexin and coated with hydroxyapatite were fabricated for bone regeneration. The scaffolds showed antibacterial properties, uniform nano-micro fibrous structure, good wettability, swelling, and biodegradation characteristics, as well as supporting cell adhesion and high alkaline phosphatase activity.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2023)
Article
Engineering, Biomedical
Hugues Mondesert, Frederic Bossard, Denis Favier
Summary: This study investigates anisotropic fibrous scaffolds fabricated by template-assisted electrospinning, showing significant differences in mechanical properties along two orthogonal directions with an anisotropic ratio of 4.2. Through tensile test experiments and stress relaxation test, the study reveals the low contribution of viscosity in the mechanical response of the scaffold. An orthotropic linear elastic model is proposed to characterize the anisotropic behavior of the patterned membranes, offering potential benefits for soft regenerative medicine applications and custom-made scaffolds.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Polymer Science
L. A. Can-Herrera, A. I. Oliva, M. A. A. Dzul-Cervantes, O. F. Pacheco-Salazar, J. M. Cervantes-Uc
Summary: The study investigates the effect of applied voltage on electrospun PCL scaffolds, finding that increasing voltage significantly affects the morphology and mechanical properties of the fibers. This has important implications for tissue engineering applications.
Article
Polymer Science
Victor Perez-Puyana, Paula Villanueva, Mercedes Jimenez-Rosado, Fernando de la Portilla, Alberto Romero
Summary: This study aimed to improve the characteristics of polycaprolactone (PCL) scaffolds by incorporating elastin, and to evaluate different cell incubation protocols to enhance the activity and functionality yields of regenerated cells. The results showed that elastin incorporation generated scaffolds with better mechanical properties and biocompatibility, suitable for use in bioreactors and patients. However, due to low maturation in biological tests, no significant differences were found between incubation protocols with and without dynamic mechanical stimulation.
Article
Polymer Science
Felipe Castro Menezes, Nataly Machado Siqueira, Stephanie Fung, Joice Maria Scheibel, Dinara Jaqueline Moura, Murat Guvendiren, Joachim Kohn, Rosane Michele Duarte Soares
Summary: Electrospinning is a versatile technique for producing composite scaffolds with nanostructure properties similar to the natural extracellular matrix. In this study, the effects of fiber alignment, mechanical properties, and chemical modifications on the physicochemical properties of composite scaffolds were investigated. The results showed that incorporating gelatin and aligning the fibers improved the wettability and mechanical strength of the material, and promoted osteoinduction.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2022)
Article
Engineering, Biomedical
Yeganeh Nikakhtar, Seyedeh Sara Shafiei, Mehrnoush Fathi-roudsari, Mitra Asadi-Eydivand, Faeze ShiraliPour
Summary: This study fabricated composite scaffolds consisting of dicalcium phosphate dihydrate (DCDP) and polycaprolactone (PCL) using electrospinning technique for bone tissue engineering. The results showed that the scaffold containing 3 wt. % of DCDP had the highest tensile strength and the addition of DCDP increased alkaline phosphatase activity and osteocalcin gene expression.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2022)
Article
Polymer Science
Diana Querido, Tania Vieira, Jose Luis Ferreira, Celia Henriques, Joao Paulo Borges, Jorge Carvalho Silva
Summary: Hybrid scaffolds obtained by combining two or more biopolymers are studied in the context of tissue regeneration. This study produced a new type of hybrid polycaprolactone (PCL)/chitosan (CS) electrospun mat by controlling the deposition of CS flakes between PCL fibers. The morphology of the resulting fibers confirmed the presence of CS flakes. In vitro cell culture results showed that the addition of CS reduced cell adhesion and proliferation.
Editorial Material
Chemistry, Physical
Anna. V. V. Taubenberger, Tanmay. P. P. Lele
Article
Engineering, Biomedical
Agathe Bessot, Jennifer Gunter, David Waugh, Judith A. Clements, Dietmar W. Hutmacher, Jacqui McGovern, Nathalie Bock
Summary: Increasing evidence highlights the important role of bone marrow adipocytes in prostate cancer bone metastases. However, lack of relevant models has hindered the understanding of human BM-adipocyte effects in this microenvironment. This study combines tunable GelMA-based hydrogels with biomimetic culture of human cells to engineer human bone tumor microenvironments containing BM-adipocytes. In vitro culture of human osteoprogenitors, adipocytes, and PCa cells in GelMA hydrogels leads to the formation of mineralized, adipose, and PCa tumor 3D microtissues, respectively. The stiffness of hydrogels regulates osteoblast mineralization and tumor spheroid formation, while coculture with tumor cells induces morphological changes and delipidation in BM-adipocytes. The in vivo model successfully forms a humanized fatty bone microenvironment and demonstrates that adipocytes support early bone tumor growth. This advanced platform combines natural aspects of the microenvironment with tunable properties for bone tumor research.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Verena Kast, Ali Nadernezhad, Dagmar Pette, Anastasiia Gabrielyan, Maximilian Fusenig, Kim C. Honselmann, Daniel E. Stange, Carsten Werner, Daniela Loessner
Summary: Pancreatic cancer is a devastating malignancy with limited treatment options. The tumor microenvironment, containing excessive extracellular matrix and stromal cells, acts as a barrier to drug delivery and contributes to therapy resistance and disease progression. To overcome this limitation, a multicellular 3D cancer model is developed to mimic key components of tumor tissues and assess responses to immunotherapy. Combining the CD11b agonist ADH-503 with anti-PD-1 immunotherapy and chemotherapy shows significant reduction in tumor cell viability, proliferation, metabolic activity, immunomodulation, and secretion of immunosuppressive and tumor growth-promoting cytokines.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Frank Schulze, Annemarie Lang, Janosch Schoon, Georgi I. I. Wassilew, Johannes Reichert
Summary: Bone has a high ability to regenerate, but large defects sometimes cannot heal. Tissue engineering and regenerative medical strategies have been researched to address this challenge. This review evaluates current approaches and discusses future advanced techniques for bone tissue engineering and their translation to clinical applications.
Article
Biochemistry & Molecular Biology
Andreas Nitsch, Sara Qarqash, Sarah Roemer, Janosch Schoon, Axel Ekkernkamp, Maya Niethard, Johannes C. Reichert, Georgi I. Wassilew, Mladen V. Tzvetkov, Lyubomir Haralambiev
Summary: This study investigated the synergistic effects of cold physical plasma (CPP) and commonly used cytostatic chemotherapeutics on Ewing's sarcoma (ES) cells. The combination of CPP and chemotherapy drugs resulted in significant growth inhibition and increased apoptosis rates in ES cells. These findings suggest that CPP could enhance the efficacy of cytostatic chemotherapeutics, supporting its potential as an anti-tumor therapy in clinical practice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Leire Moya, Carina Walpole, Fiona Rae, Srilakshmi Srinivasan, Inge Seim, John Lai, David Nicol, Elizabeth D. Williams, Judith A. Clements, Jyotsna Batra
Summary: This study successfully established four cell lines derived from localized prostate cancer, including two benign and two malignant cell lines. The characteristics and molecular events of these cell lines could help in understanding the development and progression of cancer.
PROSTATE CANCER AND PROSTATIC DISEASES
(2023)
Review
Engineering, Biomedical
Jana Sievers, Vaibhav Mahajan, Petra B. Welzel, Carsten Werner, Anna Taubenberger
Summary: Cancer progression involves remodeling of the tumor microenvironment, particularly changes in mechanical characteristics. Bioengineered polymer hydrogels can be used to explore how these mechanical changes impact cancer cell behavior. This article reviews studies using hydrogel-based models to investigate the effects of different mechanical cues on cancer cells and highlights advanced strategies for recreating the tumor microenvironment's architectural and mechanical features. It is anticipated that these precision hydrogel systems will enhance our understanding of cancer mechanobiology.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Nanoscience & Nanotechnology
Rodrigo Curvello, Verena Kast, Paloma Ordonez-Moran, Alvaro Mata, Daniela Loessner
Summary: Biology can be used to design materials and approaches for tumour tissue engineering, and biomaterials are necessary for rebuilding tissue organization, composition, and function. This review discusses bioengineering strategies that recreate the pathophysiology of tumour tissues to address questions in cancer research. Tissue engineering has produced innovative tools for cancer research.
NATURE REVIEWS MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Tara Shabab, Onur Bas, Bronwin L. Dargaville, Akhilandeshwari Ravichandran, Phong A. Tran, Dietmar W. Hutmacher
Summary: This study combines melt-extrusion based 3D printing and porogen leaching to develop tunable multiphasic scaffolds for dental tissue regeneration. The scaffolds are highly adjustable in terms of mechanical properties, degradation kinetics, and surface morphology. The research also demonstrates improved cell attachment, proliferation, and extracellular matrix production on these multiscale scaffolds compared to single-scale counterparts, indicating their potential for enhanced tissue regeneration. Furthermore, the study explores the use of these scaffolds as drug delivery devices and shows that sustained drug release can be achieved. These findings strongly support further development of these scaffolds for dental tissue regeneration applications.
Review
Oncology
Fatemeh Davoudi, Afshin Moradi, Therese M. M. Becker, John G. G. Lock, Brian Abbey, Davide Fontanarosa, Annette Haworth, Judith Clements, Rupert C. C. Ecker, Jyotsna Batra
Summary: Prostate cancer is a commonly diagnosed malignant neoplasm and a leading cause of cancer-related death in men worldwide. Personalized therapeutic strategies for this disease remain limited, but emerging biomarkers based on individual patient profiles and computer-assisted diagnostics have the potential to guide precision medicine. This review discusses screening methods, genetic aberrations, and phenotypic biomarkers for advanced prostate cancer, exploring their clinical implications in diagnosis, prognosis, and treatment prediction. Prognostic and treatment predictive biomarker discovery can improve patient management and personalized treatment regimens.
CURRENT TREATMENT OPTIONS IN ONCOLOGY
(2023)
Article
Engineering, Manufacturing
Buddhi Herath, Markus Laubach, Sinduja Suresh, Beat Schmutz, J. Paige Little, Prasad K. D. V. Yarlagadda, Dietmar W. Hutmacher, Marie-Luise Wille
Summary: A modular design workflow was developed to facilitate the efficient design and manufacture of patient-specific scaffolds for scaffold-guided bone regeneration (SGBR). The workflow allows for semi-automatic filling of defect cavities, patient specificity, ideal surgical scaffold insertion, and generation of scaffolds based on various pore architectures. The real-time feedback from surgeons shortens the time required to finalize a patient-specific scaffold design.
VIRTUAL AND PHYSICAL PROTOTYPING
(2023)
Review
Engineering, Biomedical
Markus Laubach, Frank Hildebrand, Sinduja Suresh, Michael Wagels, Philipp Kobbe, Fabian Gilbert, Ulrich Kneser, Boris M. Holzapfel, Dietmar W. Hutmacher
Summary: The treatment of bone defects is a challenging clinical problem with high reintervention rates and significant healthcare costs. Three-dimensional printing has revolutionized the treatment of bone defects by enabling personalized therapies with highly porous 3D-printed implants. However, there are still challenges to be addressed, including the development of guidelines, clinical trial design, and financing support.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Mairim Russo Serafini, Alexandra Mowat, Susanah Mustafa, Siamak Saifzadeh, Tara Shabab, Onur Bas, Nicholas O'Rourke, Dietmar W. Hutmacher, Flavia Medeiros Savi
Summary: This study evaluated the use of mPCL scaffolds loaded with PRP for hernia repair in sheep models, and found that the mPCL scaffolds enhanced soft tissue regeneration. After 6 months, the mPCL scaffolds showed similar mechanical properties, collagen, and vascular ingrowth compared to PP meshes. This pilot study supports the use of mPCL as a safe and effective biodegradable scaffold for hernia and vaginal prolapse repair.
BIOENGINEERING-BASEL
(2023)
Article
Orthopedics
Manuela Hoffmann, Johannes C. Reichert, Anastasia Rakow, Janosch Schoon, Georgi I. Wassilew
Summary: In 2020, more than 14,000 aseptic revision procedures for total hip arthroplasty (THA) were recorded in Germany. Patients' expectations for revision surgery are similar to primary hip replacement. However, revision surgery has higher complication rates and more dissatisfied patients compared to primary THA, with poorer postoperative function, increased pain levels and impaired quality of life and return-to-work.
