Review
Pharmacology & Pharmacy
Renata Maia, Violeta Carvalho, Rui Lima, Graca Minas, Raquel O. Rodrigues
Summary: This systematic review summarizes the recent progress in the use of microneedles (MNs) in biomedical applications, including drug delivery, biomarker detection, and their potential integration with microfluidic devices. Although MNs have been extensively utilized in lab-on-a-chip platforms, recent studies have also explored their applicability in organ-on-a-chip models. Overall, the presence of MNs in advanced microfluidic devices offers simplified drug delivery, microinjection, and biomarker detection through integrated biosensors, enabling real-time monitoring of various biomarkers in lab- and organ-on-a-chip platforms.
Article
Chemistry, Analytical
Canan Ozyurt, Inci Uludag, Bahar Ince, Mustafa Kemal Sezginturk
Summary: Lab-on-a-chip (LOC) or micro total analysis system is a microfluidic technology that integrates and automates analytical laboratory procedures into a single instrument or chip. This article reviews the recent developments in the application of LOC biosensors for cancer detection. Microfluidics has revolutionized healthcare diagnosis by enabling the reconstruction of functional organ units on chips and studying human diseases like cancer. In the past five years, LOCs have been widely used in various biomedical applications such as bioassays, cell analysis, drug discovery, and personalized medicine. This review provides an overview of microfluidic-based cancer research, including its pros, cons, and future prospects.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Optics
Filippo Storti, Silvio Bonfadini, Andrea Di Donato, Luigino Criante
Summary: This work presents a successful combination of femtosecond laser irradiation, CO2 laser polishing, and inkjet printing to fabricate high-quality micro-optic elements. These elements are fully integrated on fused silica substrates and can enhance the optical performance of lab on a chip devices.
Article
Optics
Jiabin Wang, Yanru Kou, Anzhi Wang, Jiayu Hao, Chong Niu, Hang Jiang, Haoran Meng, Weimin Sun, Tao Geng
Summary: This article proposes an ultra-compact Fabry-Perot interferometer (FPI) based on a laser-induced micro-cavity (LIMC-FPI) on a fiber end for measuring relative humidity. The authors introduce a fresh and cost-effective approach for developing lab-on-fiber concept based sensors.
Review
Chemistry, Analytical
Mylena Lemes Cunha, Stella Schuster da Silva, Mateus Cassaboni Stracke, Dalila Luciola Zanette, Mateus Nobrega Aoki, Lucas Blanes
Summary: Rapid and low-cost molecular analysis is crucial for early and specific diagnostics. Automated molecular diagnostic devices, such as sample-to-answer microfluidic devices, promise a complete molecular diagnosis system. The extraction process in such devices is laborious but important for sensitive and specific detection. Thus, researching automated or semiautomated extraction methodologies is beneficial for improving extraction procedures.
ANALYTICAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Ghazal Shabestani Monfared, Peter Ertl, Mario Rothbauer
Summary: Cutaneous wound healing is a complex process involving cell communication events and cell migration to achieve wound closure. Microfluidic and lab-on-a-chip technologies have improved in vitro wound healing assays to create more reliable models resembling the in vivo wound microenvironment.
Article
Computer Science, Theory & Methods
Xing Huang, Tsung-Yi Ho, Wenzhong Guo, Bing Li, Krishnendu Chakrabarty, Ulf Schlichtmann
Summary: Flow-based microfluidic biochips are increasingly utilized in biology and biochemistry for laboratory procedures, with advancements in manufacturing technology allowing for integration of thousands of microvalves into a small platform. Computer-aided design (CAD) in the past decade has attracted research interest for automation of biochip design, enabling designers to explore new chip architectures and simplify complex tasks. Key trends and directions for future research focus on maximizing the potential of microfluidics to advance the industry.
ACM COMPUTING SURVEYS
(2021)
Review
Chemistry, Multidisciplinary
Samaneh Zare Harofte, Madjid Soltani, Saeed Siavashy, Kaamran Raahemifar
Summary: Nowadays, artificial intelligence creates numerous opportunities in the life sciences. Integrating AI with microfluidics can significantly impact various fields of biotechnology.
Article
Engineering, Electrical & Electronic
Sarah E. Lu, Andrew Morris, Geraldine Clinton-Bailey, Medya Namiq, Paul C. Gow, Antony Birchill, Sebastian Steigenberger, James Wyatt, Reuben Forrester, Matthew C. Mowlem, Phillip E. Warwick
Summary: Nuclear reactor process control often relies on manual sampling and laboratory analysis for monitoring pure β-emitting radionuclides, leading to delays in data availability and response times. The development of an in situ microfluidic Lab on Chip (LoC) system with integrated detection offers a promising solution, reducing occupational exposure and monitoring costs while providing improved temporal resolution through near real-time data acquisition.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Article
Computer Science, Hardware & Architecture
Xing Huang, Youlin Pan, Zhen Chen, Wenzhong Guo, Lu Wang, Qingshan Li, Robert Wille, Tsung-Yi Ho, Ulf Schlichtmann
Summary: Due to the complexity of chip architecture and assay protocol, traditional methods for designing continuous-flow microfluidic lab-on-a-chip systems have serious gaps between different steps, leading to design failure. This article proposes a novel "one-pass design paradigm" that integrates all design steps seamlessly, eliminating the gaps and generating optimized biochip architectures without the need for design adjustments and modifications. Experimental results demonstrate the effectiveness of the proposed automation flows.
IEEE TRANSACTIONS ON COMPUTER-AIDED DESIGN OF INTEGRATED CIRCUITS AND SYSTEMS
(2023)
Review
Nanoscience & Nanotechnology
Jens Ducree
Summary: This review discusses the challenges and current trends in larger-scale integration of microfluidic systems, highlighting the advantages of centrifugal systems for sample preparation. It also introduces a method using simulation tools for virtual prototyping and design optimization.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Biochemical Research Methods
Ning Zhang, Chao Yue, Xiaobo Zhan, Zhi Cheng, Chao Li, Yaohua Du, Feng Tian
Summary: The quantitative analysis of respiratory viruses is important for diagnosis, medicine and prognosis. In this study, a lab-on-a-chip platform combined with an automated control system was used to achieve quantitative analysis of COVID-19 pseudovirus from large-volume nasal swab samples. The method can be extended to other respiratory viruses and sample types.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Alexandro Catini, Rosamaria Capuano, Giuseppe Tancredi, Giulio Dionisi, Davide Di Giuseppe, Joanna Filippi, Eugenio Martinelli, Corrado Di Natale
Summary: This paper presents an automatic chemical assay platform for continuous monitoring of harmful compounds in aquaculture. The platform uses a lab-on-a-chip approach and low-cost optoelectronic components to analyze water samples, providing a reliable and cost-effective solution.
Article
Multidisciplinary Sciences
Sangam Srikanth, U. S. Jayapiriya, Satish Kumar Dubey, Arshad Javed, Sanket Goel
Summary: A simple, cost-effective and miniaturized lab-on-a-chip platform has been developed for simultaneous cultivation and detection of bacteria, with high accuracy in quantifying bacterial concentration and validating viability, as well as real-time and on-field applications.
Article
Materials Science, Multidisciplinary
Zhenzhen Chen, Liang Shen, Xiong Zhao, Hongqiang Chen, Yaxuan Xiao, Yonghai Zhang, Xiaoping Yang, Jinhua Zhang, Jinjia Wei, Nanjing Hao
Summary: Microfluidics, especially acoustofluidics, offers new opportunities in chemical and biomedical engineering by manipulating fluids at the micrometer or submicrometer scale. This review discusses the utility of acoustofluidic technology, summarizes different types of acoustic micromixers, highlights their applications in various fields, and identifies the practical challenges that need to be addressed. Overall, this review provides valuable insights for both academic research and industrial applications.
APPLIED MATERIALS TODAY
(2022)
Article
Engineering, Multidisciplinary
Joerg Koenig, Juergen Czarske
MEASUREMENT SCIENCE AND TECHNOLOGY
(2017)
Article
Engineering, Multidisciplinary
S. Moller, J. Koenig, C. Resagk, C. Cierpka
MEASUREMENT SCIENCE AND TECHNOLOGY
(2019)
Article
Nanoscience & Nanotechnology
Wiebke Roesing, Toni Schildhauer, Joerg Koenig, Christian Cierpka
MICROFLUIDICS AND NANOFLUIDICS
(2019)
Article
Thermodynamics
Joerg Koenig, Sebastian Moller, Nicolai Granzow, Christian Cierpka
EXPERIMENTAL THERMAL AND FLUID SCIENCE
(2019)
Article
Engineering, Multidisciplinary
Joerg Koenig, Minqian Chen, Wiebke Roesing, David Boho, Patrick Maeder, Christian Cierpka
MEASUREMENT SCIENCE AND TECHNOLOGY
(2020)
Article
Engineering, Mechanical
Theo Kaeufer, Joerg Koenig, Christian Cierpka
Summary: This paper presents a method of using low-cost action cameras for stereoscopic planar PIV, minimizing systematic errors by adjusting light pulsation and camera frame rate, and allowing for post-processing synchronization. The rugged nature of action cameras enables stereoscopic PIV in harsh environments and moving experiments.
EXPERIMENTS IN FLUIDS
(2021)
Article
Engineering, Multidisciplinary
Zhichao Deng, Jorg Koenig, Christian Cierpka
Summary: APTV combined with luminescence lifetime imaging can measure the temperature and velocity components in a volume through a single optical access, using an LED instead of a pulsed laser to prevent additional heating, and replacing a high-speed camera with an ordinary double-frame camera for cost efficiency, making it a suitable measurement technique for microfluidic research.
MEASUREMENT SCIENCE AND TECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Sebastian Bohm, Hai Binh Phi, Ayaka Moriyama, Erich Runge, Steffen Strehle, Jorg Konig, Christian Cierpka, Lars Dittrich
Summary: A multistage optimization method is developed to create efficient Tesla valves at low flow rates. The method uses topological optimization based on the finite element method, resulting in compact valve structures that can be directly used in microfluidic systems.
MICROSYSTEMS & NANOENGINEERING
(2022)
Article
Engineering, Mechanical
Sebastian Sachs, Manuel Ratz, Patrick Maeder, Jorg Koenig, Christian Cierpka
Summary: The astigmatism particle tracking velocimetry is extended by a deterministic algorithm and a deep neural network (DNN) to include size classification of particles of multimodal size distribution. By extracting particle images from the experimentally acquired images and placing them on a synthetic background, semi-synthetic images with consistent ground truth are generated. The present contribution paves the way for quantitative evaluation of microfluidic separation and mixing processes.
EXPERIMENTS IN FLUIDS
(2023)
Article
Engineering, Multidisciplinary
M. Ratz, S. Sachs, J. Koenig, C. Cierpka
Summary: In this study, deep neural networks (DNNs) were used to extract the class and position of particles in both the plane and depth. The performance of DNNs was evaluated on synthetic and experimental images, showing high accuracy and precision in 3D particle tracking.
MEASUREMENT SCIENCE AND TECHNOLOGY
(2023)
Article
Engineering, Electrical & Electronic
Raschid Azizy, Henning Otto, Joerg Koenig, David Schreier, Christoph Weigel, Christian Cierpka, Steffen Strehle
Summary: This paper discusses the design and fabrication of a magnetohydrodynamic micropump using patterned glass and dry film photoresist. The magnetohydrodynamic pumping effect is achieved through the superposition of an electric ion current and an external magnetic field. The microchannels are sealed using a thermally bonded laser-structured glass lid. The micropump serves as a technology demonstrator for further research of microfluidic on-chip micropumps and other microfluidic systems.
MICRO AND NANO ENGINEERING
(2023)
Article
Biochemical Research Methods
Zhichao Deng, Vijay V. Kondalkar, Christian Cierpka, Hagen Schmidt, Joerg Koenig
Summary: It has been found through experimental studies that the pattern formed by individual particles trapped in a microfluidic chamber by a two-dimensional standing acoustic wave field cannot solely be attributed to the acoustic radiation force. The particles are trapped at the local minima and maxima of the first-order pressure and velocity fields, resulting in rectangular or diamond patterns depending on the particle size and material properties. Co-existence of different patterns with particles of the same size is also observed. The actual shape of the patterns depends on the ratio between particle diameter and wavelength, and particles can be trapped at antinode positions despite having a positive acoustic contrast factor. These findings suggest that the trapping of individual particles cannot be explained solely by the acoustic radiation force, and the viscous drag force caused by acoustic streaming needs to be considered.
Article
Biochemical Research Methods
Robert Weser, Zhichao Deng, Vijay V. Kondalkar, Alexandre N. Darinskii, Christian Cierpka, Hagen Schmidt, Joerg Koenig
Summary: Acoustic tweezers provide a noninvasive, contactless method for precise manipulation of micro objects, including biological cells. This study reveals the correlation between acoustic fields, acoustophoretic motion and heating effects, and demonstrates three-dimensional particle patterning and rapid heating.
Article
Biochemical Research Methods
Sebastian Sachs, Mostafa Baloochi, Christian Cierpka, Joerg Koenig
Summary: By integrating surface acoustic waves (SAW) into microfluidic devices, microparticle systems can be precisely fractionated on flexible Lab-on-a-Chip platforms. This study reveals the fluid flow induced by pseudo-standing acoustic wave fields and identifies critical parameters for acoustofluidic separation system design.
Article
Biochemical Research Methods
Sebastian Sachs, Christian Cierpka, Joerg Koenig
Summary: This study provides a deep insight into the influence of acoustically induced flow on the fractionation of suspended particles using quantitative three-dimensional velocity measurements. The results show that broadly extended vortices are formed due to large gradients in the acoustic fields, which are essential for a holistic analysis of the flow phenomena.
