Article
Pharmacology & Pharmacy
Minhui Yang, Changxiao Hu, Yibo Cao, Wanling Liang, Xiangdong Yang, Tianbao Xiao
Summary: The study explores the biological functions of CCNB1 in COAD and investigates the therapeutic effects of UA on COAD cells. UA effectively inhibits COAD cell proliferation and induces cell cycle arrest in S phase. The molecular mechanisms of UA involve downregulation of CCNB1 and its interacting genes and proteins, contributing to cell cycle blocking and COAD treatment.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Riham R. E. Abouleisa, Jessica M. Miller, Ahmad Gebreil, Abou Bakr M. Salama, Marc Dwenger, Hania Abdelhafez, Reham M. Wahid, Adeniyi T. Adewumi, Mahmoud E. S. Soliman, Nader E. Abo-Dya, Tamer M. A. Mohamed
Summary: This study identified a small molecule called NDPPC, which can enhance the cell cycle progression of cardiomyocytes and has the potential to be a drug for heart failure treatment.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Betheney R. Pennycook, Alexis R. Barr
Summary: The use of CDK4/6 inhibitors in cancer treatment is a topic of ongoing investigation, with limited understanding of their mechanisms of action. Recent data have raised questions about how these inhibitors arrest cell proliferation, leading to renewed interest in the roles of CDK4/6. Studying the effects of CDK4/6 inhibitors on the cell cycle can have implications for cancer treatment and patient stratification.
Article
Environmental Sciences
Mario Alberto Burgos Aceves, Vincenzo Migliaccio, Marilena Lepretti, Gaetana Paolella, Ilaria Di Gregorio, Serena Penna, Caterina Faggio, Lilla Lionetti
Summary: The study revealed that DDE has a dose-dependent impact on cell viability and mitochondrial fusion/fission proteins, with high doses leading to decreased cell viability and increased fission/fusion ratio. Low doses of DDE stimulated cell adaption and altered mitochondrial dynamics.
Article
Biochemistry & Molecular Biology
Marian Kratzke, George Scaria, Stephen Porter, Betsy Kren, Mark A. Klein
Summary: Advanced mesothelioma is an incurable disease, and new treatment strategies are needed. This study demonstrated that the inhibitors auranofin and palbociclib could effectively decrease mesothelioma cell proliferation and inhibit activity related to antioxidant defense and cell cycle progression. Further investigation of this drug combination may reveal a new treatment strategy for mesothelioma.
Article
Pharmacology & Pharmacy
Kaori Ishikawa, Yo Honma, Ayami Yoshimi, Shun Katada, Takaya Ishihara, Naotada Ishihara, Kazuto Nakada
Summary: Regulation of mitochondrial respiration and morphology is crucial for maintaining hematopoiesis. This study found that abnormal mitochondrial respiration or dynamics in mouse models can cause anemia, with different manifestations. When both deficits occur simultaneously, it amplifies abnormal iron metabolism and worsens anemia.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Kei Takuma, Shintaro Fujihara, Koji Fujita, Hisakazu Iwama, Mai Nakahara, Kyoko Oura, Tomoko Tadokoro, Shima Mimura, Joji Tani, Tingting Shi, Asahiro Morishita, Hideki Kobara, Takashi Himoto, Tsutomu Masaki
Summary: This study investigates the antitumor mechanism of regorafenib on hepatocellular carcinoma (HCC) and the impact on microRNA (miRNA) expression. The results suggest that regorafenib inhibits cell proliferation and tumor growth in HCC by altering the cell cycle and miRNA expression, particularly downregulating cyclin D1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Minal S. Ayachit, Bhupendra Shravage
Summary: Mitochondrial dynamics play an important role in stem cell maintenance and differentiation. A study in Drosophila reveals that Atg1 is involved in mitophagy, autophagy and mitochondrial fusion, and its depletion affects the maintenance and development of female germline stem cells. The interaction between Atg1, Drp1, and Marf influences the fate of these stem cells and the process of oogenesis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
RuoLi Chen, Mohammad Alkataan Ahmed, Nicholas Robert Forsyth
Summary: Cells respond to reduced oxygen availability primarily through activation of the HIF pathway, which upregulates genes for cell survival. This study investigated the effects of DMOG on PC12 cells cultured in varying oxygen levels, finding that DMOG stabilises HIF and affects mitochondrial activity and cell behaviour, but does not accurately replicate the reduced oxygen environment.
Article
Biochemistry & Molecular Biology
Lei Xu, Yanpin Li, Zixi Wei, Rong Bai, Ge Gao, Wenjuan Sun, Xianren Jiang, Junjun Wang, Xilong Li, Yu Pi
Summary: This study found that CDCA can promote the proliferation of IPEC-J2 cells by regulating cell cycle progression and mitochondrial function.
Article
Biochemistry & Molecular Biology
Atsuhiro Tsutiya, Mitsumi Arito, Takuma Tagashira, Masaaki Sato, Kazuki Omoteyama, Toshiyuki Sato, Naoya Suematsu, Manae S. Kurokawa, Tomohiro Kato
Summary: In this study, we found that layilin localized to mitochondria and played a role in the maintenance of fragmented mitochondria in mitochondrial dynamics, which required activation of CDK1 and DRP1. Our data revealed a novel function for layilin, regulation of mitochondrial dynamics.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Immunology
Yuanyuan Zhou, Ming Li, Zhenglei Wang, Xinguang Lin, Yuanyuan Xu, Shiyuan Feng, Jinfeng Miao
Summary: Excessive production of ROS leads to oxidative stress, affecting disease progression. Mitochondria are a significant source of ROS, and their dysfunction is closely associated with ROS production. S. uberis infection disrupts mitochondrial dynamics, leading to fragmentation and functional decline. The AMPK activator AICAR improves mitochondrial dysfunction induced by S. uberis infection by inhibiting Drp1 phosphorylation and translocation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Gastroenterology & Hepatology
Huey-Huey Chua, Mei-Hwei Chang, Ya-Hui Chen, Daw-Jen Tsuei, Yung-Ming Jeng, Po-Huang Lee, Yen-Hsuan Ni
Summary: We identified RBMY as a predictive biomarker for hepatocellular carcinoma metastasis. Provirus integration phosphorylates RBMY, leading to stabilized and translocated RBMY that promotes epithelial-mesenchymal transition and mitochondrial dysregulation in hepatocellular carcinoma, resulting in distant metastasis.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Cell Biology
Rong Yu, Shao-Bo Jin, Maria Ankarcrona, Urban Lendahl, Monica Nister, Jian Zhao
Summary: Drp1 exists as a mixture of self-assembly forms in mammalian cells, and its assembly state affects its binding with MIEFs and Mff. Mff binds preferentially to higher order oligomers of Drp1, while MIEFs interact with a wider range of Drp1 assembly subunits.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Luca Simula, Ylenia Antonucci, Giorgia Scarpelli, Valeria Cancila, Alessandra Colamatteo, Simona Manni, Biagio De Angelis, Concetta Quintarelli, Claudio Procaccini, Giuseppe Matarese, Claudio Tripodo, Silvia Campello
Summary: The PD-1 signaling pathway downregulates T-cell response by inhibiting Drp1 activity, leading to reduced motility and proliferation of tumor-infiltrating T cells. This highlights the potential of targeting Drp1 activity as a valuable strategy to enhance anticancer immune response in future immunotherapy approaches.
MOLECULAR ONCOLOGY
(2022)
Article
Oncology
Deepak Kumar Tanwar, Danitra J. Parker, Priyanka Gupta, Brian Spurlock, Ronald D. Alvarez, Malay Kumar Basu, Kasturi Mitra
Article
Multidisciplinary Sciences
Danitra J. Parker, Aida Moran, Kasturi Mitra
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2017)
Article
Developmental Biology
Darshika Tomer, Rohan Chippalkatti, Kasturi Mitra, Richa Rikhy
DEVELOPMENTAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Matthew Redmann, Gloria A. Benavides, Willayat Yousuf Wani, Taylor F. Berryhill, Xiaosen Ouyang, Michelle S. Johnson, Saranya Ravi, Kasturi Mitra, Stephen Barnes, Victor M. Darley-Usmar, Jianhua Zhang
Article
Cell Biology
Brian Spurlock, Priyanka Gupta, Malay Kumar Basu, Avik Mukherjee, Anita B. Hjelmeland, Victor Darley-Usmar, Danitra Parker, McKenzie E. Foxall, Kasturi Mitra
JOURNAL OF CELL SCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Archana Iyer, Renata A. G. Reis, Swathi Gannavaram, Mohamed Momin, Alexander M. Spring-Connell, Yoelvis Orozco-Gonzalez, Johnson Agniswamy, Donald Hamelberg, Irene T. Weber, Samer Gozem, Siming Wang, Markus W. Germann, Giovanni Gadda
Summary: This study demonstrated how an active site residue changes enzyme reactivity by modulating fluctuations between conformational states, resulting in an enzyme with different activities. The replacement of tyrosine 249 with phenylalanine in the active site yielded an enzyme variant with both active and inactive FAD, showing no structural differences compared to the wild-type enzyme. Molecular dynamics simulations revealed an alternate conformation in the variant enzyme that samples a metastable state and has differences in flavin electronics compared to the wild type.
Article
Gastroenterology & Hepatology
Trung Vu, Arunima Datta, Carolyn Banister, Lin Jin, Guandou Yuan, Temesgen Samuel, Sejong Bae, Isam-Eldin Eltoum, Upender Manne, Bixiang Zhang, Robert S. Welner, Kasturi Mitra, Phillip Buckhaults, Pran K. Datta
Summary: The study revealed that STRAP plays a crucial role in Apc mutation/deletion-induced intestinal tumorigenesis through a novel feed-forward regulatory axis involving STRAP/MEK-ERK/Wnt-beta-catenin/STRAP. This indicates a potential therapeutic target for colorectal cancer associated with Apc gene mutations.
Article
Biology
Brian Spurlock, Danitra Parker, Malay Kumar Basu, Anita Hjelmeland, G. C. Sajina, Shanrun Liu, Gene P. Siegal, Alan Gunter, Aida Moran, Kasturi Mitra
Summary: The fine-tuned repression of the mitochondrial fission regulator Drp1 supports a slower cycling 'stem/progenitor-like state' and is crucial for neoplastic transformation. Fine tuning Drp1 protein levels can maintain stem/progenitor cell markers and accelerate or prevent neoplastic transformation based on the level of reduction.
Article
Biochemistry & Molecular Biology
Archana Iyer, Renata A. G. Reis, Johnson Agniswamy, Irene T. Weber, Giovanni Gadda
Summary: The crystal structure of D-arginine dehydrogenase from Pseudomonas aeruginosa has been determined, revealing the versatility of the reduced flavin in performing multiple functions at the same active site.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Cell Biology
Qiancong Zhao, Shun Yan, Jin Lu, Danitra J. Parker, Huiying Wu, Qianchuang Sun, David K. Crossman, Shanrun Liu, Qin Wang, Hiromi Sesaki, Kasturi Mitra, Kexiang Liu, Kai Jiao
Summary: Mitochondrial dysfunction leads to severe cardiac abnormalities and prenatal/neonatal lethality. The lack of understanding of how mitochondrial abnormalities affect cardiac development hinders the development of clinical applications for inborn cardiomyopathies caused by mitochondrial deficiency. By studying a model related to the role of Drp1 in cardiomyocyte development, we uncover the mechanisms by which mitochondrial dysfunction causes pathological molecular and cellular changes during cardiac development.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemical Research Methods
Swati Agarwala, Mayank Saini, Danitra Parker, Kasturi Mitra
Summary: This paragraph introduces a protocol for enriching and identifying mitochondria-primed stem cells and describes the steps for enriching mpSCs in a skin keratinocyte lineage and identifying them using single-cell transcriptomics and single-cell microscopy analysis.
Article
Biology
Brian Spurlock, Vidya Sagar Hanumanthu, Kasturi Mitra
Summary: Stem cells and stem-like tumor initiating cells have been shown to rely on mitochondrial energetics, with a sub population of cells primed by mitochondria for self-renewal. A protocol using Fluorescence-Assisted Cell Sorting has been developed to identify and isolate these primed cells, enriching TICs with higher mitochondrial potential. This protocol can be valuable for understanding mitochondrial heterogeneity within stem cell population and may have applications in regenerative medicine and cancer biology research.
Review
Biochemistry & Molecular Biology
Daniel Ouedraogo, Jacob Ball, Archana Iyer, Renata A. G. Reis, Maria Vodovoz, Giovanni Gadda
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2017)
Article
Cell Biology
Matthew Dodson, Willayat Y. Wani, Matthew Redmann, Gloria A. Benavides, Michelle S. Johnson, Xiaosen Ouyang, Stacey S. Cofield, Kasturi Mitra, Victor Darley-Usmar, Jianhua Zhang