Article
Cell Biology
Xing Xu, Jun Chen, Yan Li, Xiaojie Yang, Qing Wang, Yanjun Wen, Ming Yan, Jianjun Zhang, Qin Xu, Yan Wei, Wantao Chen, Xu Wang
Summary: Through genomics, metabolomics, and RNA omics studies, it was discovered that inhibition of squalene epoxidase synergistically increased the sensitivity of HNSCC cells to EZH2 inhibitors, providing a basis for the development of new therapeutic strategies for HNSCC.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Johannes Routila, Xi Qiao, Jere Weltner, Juha K. Rantala, Timo Carpen, Jaana Hagstrom, Antti Makitie, Ilmo Leivo, Miia Ruuskanen, Jenni Soderlund, Marjut Rintala, Sakari Hietanen, Heikki Irjala, Heikki Minn, Jukka Westermarck, Sami Ventela
Summary: This study identifies OCT4 as a potential biomarker to predict the therapeutic effect of treatment for head and neck squamous cell carcinoma (HNSCC). OCT4 expression in HNSCC tumors is associated with radioresistance, but combination therapy with cisplatin can overcome this resistance. The results of the study are validated using multiple independent patient cohorts.
Review
Medicine, General & Internal
Kamila Romanowska, Agnieszka Sobecka, Agnieszka A. Rawluszko-Wieczorek, Wiktoria M. Suchorska, Wojciech Golusinski
Summary: Head and neck squamous carcinoma (HNSCC) is the sixth most prevalent cancer worldwide, with molecular pathogenesis involving cell cycle disorders, intercellular signaling, proliferation, squamous cell differentiation, and apoptosis. In addition to genetic mutations, epigenetic alterations such as DNA methylation and histone modifications play a key role in promoting cancer formation and progression, making them potential biomarkers and therapeutic targets for HNSCC.
Article
Oncology
Johannes Routila, Karri Suvila, Reidar Grenman, Ilmo Leivo, Jukka Westermarck, Sami Ventela
Summary: In this study, the Cell Line Microarray (CMA) method was used to enhance immunohistochemical screening for potential radioresistance biomarkers in HNSCC cell lines. The results showed that Oct4 nuclear positivity and NDFIP1 nuclear positivity were correlated with increased intrinsic radioresistance. CMA-based antibody screening proved to be an efficient and relatively simple method to identify potential radioresistance biomarkers in HNSCC.
Article
Oncology
Zongtai Qi, Yating Liu, Michael Mints, Riley Mullins, Reilly Sample, Travis Law, Thomas Barrett, Angela L. Mazul, Ryan S. Jackson, Stephen Y. Kang, Patrik Pipkorn, Anuraag S. Parikh, Itay Tirosh, Joseph Dougherty, Sidharth V. Puram
Summary: Tumors are composed of diverse cells rather than a uniform ball of cells. Recent studies have shown that deconvolution algorithms can be used to computationally estimate cell-type proportions from gene expression data, although their performance in tumor tissues remains unclear.
Review
Oncology
Kainan Wu, Fen Chang, Wenming Li, Tongdong Su, Dapeng Lei
Summary: IGF2BPs are RNA-bound proteins that play a crucial part in various cell functions and act as members of the m6A reader family. They are particularly important in tumor metabolism, specifically in head and neck squamous cell carcinoma.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Avantika Agrawal, Vaibhav Vindal
Summary: Our understanding of RNA biology has shifted from considering it as non-functional to recognizing its regulatory functions within the genome. Competitive endogenous RNA (ceRNA) is an essential part of post-transcriptional regulation where coding and non-coding RNA co-regulate each other through microRNA response elements. Dysregulation of the ceRNA network has been implicated in various cancers, including head and neck squamous cell carcinoma (HNSCC). Understanding the holistic progression of HNSCC mediated by ceRNA can aid in the identification of novel biomarkers and therapeutic interventions.
BRIEFINGS IN FUNCTIONAL GENOMICS
(2023)
Article
Cell Biology
Erliang Guo, Xionghui Mao, Xueying Wang, Lunhua Guo, Changming An, Cong Zhang, Kaibin Song, Guohui Wang, Chunbin Duan, Xiwei Zhang, Xianguang Yang, Zhennan Yuan, Ji Sun, Xiaomei Li, Weiwei Yang, Hongxue Meng, Susheng Miao
Summary: High expression of ANLN is associated with poor prognosis in HNSCC patients. The two major splice variants of ANLN, ANLN-201 and ANLN-210, are highly expressed in HNSCC tissues and cell lines. ANLN promotes tumor growth and invasion in HNSCC by interacting with c-Myc and hnRNPC in different mechanisms.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Yin-Quan Chen, Chen-Yu Hung, Ming-Tzo Wei, Jean-Cheng Kuo, Muh-Hwa Yang, Han-Ying Cheng, Arthur Chiou
Summary: In this study, the researchers compared the biomolecular and biomechanical properties of lymph node (LN)-metastatic and non-metastatic cells in head and neck squamous cell carcinoma. They found that LN-metastatic cells had a higher level of Snail expression, which promoted cellular invasion capability in confined environments by increasing longitudinal strain of the cell nuclei through stronger cell traction force and softer nuclear stiffness. These biomechanical changes were correlated with a larger amount of focal adhesion and less amount of nuclear lamins. Overall, this study reveals the key process in LN metastasis by examining the biomechanical profiles and corresponding biomolecular expressions of LN-metastatic cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiunn-Min Shieh, Ting-Wei Chang, Jing-He Wang, Song-Ping Liang, Pei-Lu Kao, Liang-Yi Chen, Chia-Jui Yen, Yun-Ju Chen, Wen-Chang Chang, Ben-Kuen Chen
Summary: This study reveals the correlation between RNA-binding proteins (RBPs), specifically nucleolin (NCL) and heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2B1), and the mesenchymal phenotype of head and neck squamous cell carcinoma (HNSCC). The RBPs regulate fibronectin, an epithelial-mesenchymal transition (EMT) marker, through the ERK and NF-κB pathway, enhancing tumor cell metastasis. The RBPs/fibronectin axis plays a crucial role in EGF-induced tumor-endothelial cell interactions and HNSCC metastasis.
Review
Pharmacology & Pharmacy
Xing-xing Huo, Shu-jie Wang, Hang Song, Ming-de Li, Hua Yu, Meng Wang, Hong-xiao Gong, Xiao-ting Qiu, Yong-fu Zhu, Jian-ye Zhang
Summary: Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer malignancy worldwide and is known to have poor prognosis. RNA modifications play a role in various pathophysiological processes, including tumor development and inflammation. Adenosine-related RNA modifications have been linked to cancer and may play a role in cancer occurrence and development.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Oncology
Yoelsis Garcia-Mayea, Lisandra Benitez-Alvarez, Almudena Sanchez-Garcia, Marina Bataller, Osmel Companioni, Cristina Mir, Sergi Benavente, Juan Lorente, Nuria Canela, Ceres Fernandez-Rozadilla, Angel Carracedo, Matilde E. LLeonart
Summary: Treatment failures in HNSCC patients are often caused by the presence of resistant cells. This study used RNA sequencing and proteomics to understand the molecular mechanisms of chemoresistance in HNSCC and identified a potential protein target, MAGEB2, for sensitizing resistant patients to therapy.
Article
Biochemistry & Molecular Biology
Laura Koll, Desiree Guel, Manal I. Elnouaem, Hanaa Raslan, Omneya R. Ramadan, Shirley K. Knauer, Sebastian Strieth, Jan Hagemann, Roland H. Stauber, Aya Khamis
Summary: The expression of VDR in head and neck cancer tumors is correlated with patients' clinical parameters. The differentiation levels of tumors are negatively correlated with the expression levels of VDR and Ki67. The serum levels of vitamin D vary among patients with different levels of tumor differentiation, and female patients have a higher incidence of vitamin D insufficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Zhimou Cai, Bingjie Tang, Lin Chen, Wenbin Lei
Summary: This study identified a prognostic mast cell gene signature (MCS) using single-cell RNA sequencing analysis to assess the prognosis and immunotherapy response of patients with head and neck squamous cell carcinoma (HNSCC). The MCS effectively distinguished survival outcomes and could be used as a predictive indicator for immunotherapy response and decision-making.
Article
Dentistry, Oral Surgery & Medicine
Yanting Wang, Xianyue Ren, Weiyu Li, Ruoyan Cao, Suyang Liu, Laibo Jiang, Bin Cheng, Juan Xia
Summary: SPDEF acts as a tumor suppressor in HNSCC by transcriptionally activating NR4A1, leading to inhibition of AKT, MAPK, and NF-kappa B signaling pathways. Low expression of SPDEF is associated with poor clinical outcomes in HNSCC patients, and restoration of SPDEF inhibits cell proliferation in HNSCC.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2021)
Review
Medicine, General & Internal
Tzu-Han Yang, Chian-Ying Chou, Yi-Fan Yang, Chian-Shiu Chien, Aliaksandr A. Yarmishyn, Tzu-Ying Yang, Cheng-Hsuan Liu, Kao-Jung Chang, Yi-Ping Yang, Yuh-Lih Chang
Summary: The meta-analysis included 9 trials with 4112 patients. The results indicated that the HCQ-AZI combination increased mortality rate in COVID-19 patients, but also showed benefits in terms of viral clearance. HCQ-alone treatment showed potential benefits in decreasing mortality rate among severely infected COVID-19 patients. Further high-quality studies with longer follow-up periods are needed to confirm these findings.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2021)
Article
Medicine, General & Internal
Kai-Chun Wang, Deng-Ho Yang, Ku- Chang, Wei-Tzu Hsu, Chih-Yen Chang, Chien-Ying Wang, Yi-Ping Yang, Chian-Shiu Chien, Meng-Yin Yang, Chiung-Chyi Shen
Summary: This study evaluated the efficacy and safety of PPSV for patients with PSL, showing that PPSV treatment can effectively reduce pain and improve quality of life. However, some patients still required revision surgery after PPSV.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2021)
Article
Biochemistry & Molecular Biology
Hui-Yung Song, Yi-Ping Yang, Yueh Chien, Wei-Yi Lai, Yi-Ying Lin, Shih-Jie Chou, Mong-Lien Wang, Chien-Ying Wang, Hsin-Bang Leu, Wen-Chung Yu, Chian-Shiu Chien
Summary: This study established isogenic control iPSCs to investigate the pathogenic factors for FD phenotypes, and found phenotypic abnormalities in FD-iPSC-derived endothelial cells. These abnormalities were rescued in corrected FD-iPSC-derived endothelial cells, highlighting the critical role of endothelial cells in FD-associated vascular dysfunctions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Henkie Isahwan Ahmad Mulyadi Lai, Shih-Jie Chou, Yueh Chien, Ping-Hsing Tsai, Chian-Shiu Chien, Chih-Chien Hsu, Ying-Chun Jheng, Mong-Lien Wang, Shih-Hwa Chiou, Yu-Bai Chou, De-Kuang Hwang, Tai-Chi Lin, Shih-Jen Chen, Yi-Ping Yang
Summary: This study identified the expression of ACE2 and TMPRSS2 in human induced pluripotent stem cell (hiPSC)-derived retinal organoids, and demonstrated the potential infection of SARS-CoV-2 pseudovirus in retinal organoids and monolayer cultures. These findings highlight the potential of iPSC-derived retinal organoids as models for ACE2 receptor-based SARS-CoV-2 infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Chian-Shiu Chien, Julie Yi-Shuan Li, Yueh Chien, Mong-Lien Wang, Aliaksandr A. Yarmishyn, Ping-Hsing Tsai, Chi-Chang Juan, Phu Nguyen, Hao-min Cheng, Teh-Ia Huo, Shih-Hwa Chiou, Shu Chien
Summary: The study identified the important role of N-6-methyladenosine (m(6)A) RNA methylation in mechanotransduction in endothelial cells, with METTL3 as a central player in response to hemodynamic forces and atherogenic stimuli. The METTL3-mediated RNA hypermethylation regulated up-regulation of the NLRP1 transcript and downregulation of the KLF4 transcript through the YTHDF1 and YTHDF2 m(6)A reader proteins. Knockdown of METTL3 via repetitive shRNA administration prevented the atherogenic process, NLRP3 up-regulation, and KLF4 down-regulation in an in vivo atherosclerosis model.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Chiu-Yang Lee, Chih-Heng Huang, Elham Rastegari, Vimalan Rengganaten, Ping-Cheng Liu, Ping-Hsing Tsai, Yuan-Fan Chin, Jing-Rong Wu, Shih-Hwa Chiou, Yuan-Chi Teng, Chih-Wei Lee, Yanwen Liang, An-Yu Chen, Shu-Chen Hsu, Yi-Jen Hung, Jun-Ren Sun, Chian-Shiu Chien, Yueh Chien
Summary: Using iPSC-derived cardiomyocytes as a model, the study found that SARS-CoV2 infection can cause cardiomyocyte damage. Pretreatment with TNF-alpha enhances SARS-CoV2 entry, indicating a potential role in cytokine storm and aggravation of myocardial damage in COVID-19 patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, General & Internal
Ding-Hao Liu, Ying-Chun Jheng, Po-Yin Chen, Aliaksandr A. Yarmishyn, Shih-Eng Haung, Chian-Shiu Chien, Pin-Hsing Tsai, Yueh Chien, Yi-Ping Yang, Chung-Lan Kao
Summary: The role of exosomes in osteoarthritis has garnered attention for its potential in providing diagnostic and monitoring value through a relatively noninvasive liquid biopsy procedure. Certain miRNAs secreted in exosomes of OA patients and exosomes derived from platelet-rich plasma have shown to induce chondrogenic gene expression in OA chondrocytes, indicating their potential applications in treating OA.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2021)
Article
Cell Biology
Chian-Shiu Chien, Yueh Chien, Yi-Ying Lin, Ping-Hsing Tsai, Shih-Jie Chou, Aliaksandr A. Yarmishyn, Elham Rastegari, Ting-Xian Wang, Hsin-Bang Leu, Yi-Ping Yang, Mong-Lien Wang, Ying-Chun Jheng, Henkie Isahwan Ahmad Mulyadi Lai, Lo-Jei Ching, Teh-Ia Huo, Jong-Yuh Cherng, Chien-Ying Wang
Summary: In this study, HDT-conjugated PU-PEI600-Mal was designed for the simultaneous delivery of CRISPR/Cas9 components, achieving efficient gene correction in the model of Fabry cardiomyopathy harboring specific mutations. This method showed no cytotoxicity and successfully rescued disease features in FC-iPSC-differentiated cardiomyocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Afeez Adekunle Ishola, Chian-Shiu Chien, Yi-Ping Yang, Yueh Chien, Aliaksandr A. Yarmishyn, Ping-Hsing Tsai, Jerry Chieh-Yu Chen, Po-Kuei Hsu, Yung-Hung Luo, Yuh-Min Chen, Kung-Hou Liang, Yuan-Tzu Lan, Teh-Ia Huo, Hsin- Ma, Ming-Teh Chen, Mong-Lien Wang, Shih-Hwa Chiou
Summary: Lung cancers, particularly non-small cell lung cancers, have a poor prognosis. A circular noncoding RNA called C190 has been identified as a negative prognostic biomarker for lung cancer, and this study explores its mechanistic function and potential as a therapeutic target for NSCLC.
Article
Biochemistry & Molecular Biology
Yung-Hung Luo, Yi-Ping Yang, Chian-Shiu Chien, Aliaksandr A. Yarmishyn, Afeez Adekunle Ishola, Yueh Chien, Yuh-Min Chen, Ping-Hsing Tsai, Tzu-Wei Lin, Mong-Lien Wang, Shih-Hwa Chiou
Summary: Lung cancer is the leading cause of death from cancer in Taiwan and worldwide. Immunotherapy has shown promising efficacy in non-small cell lung cancer (NSCLC) by blocking the PD-1/PD-L1 signaling pathway. Circular RNAs (circRNAs) have been identified as potential blood-based biomarkers to monitor the disease progression and immunotherapy efficacy. Hsa_ circ_0000190 was found to interfere with anti-PD-L1 antibody and T-cell activation, leading to immunotherapy resistance and poor prognosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, General & Internal
Fang-Yu Liou, Kun-Chang Lin, Chian-Shiu Chien, Wan-Ting Hung, Yi-Ying Lin, Yi-Ping Yang, Wei-Yi Lai, Tzu-Wei Lin, Shu-Hung Kuo, Wei-Chun Huang
Summary: Out-of-hospital cardiac arrest is a leading cause of death worldwide, with bystander CPR playing a crucial role in improving survival rates. Strategies such as community interventions and real-time feedback can help increase the prevalence and quality of bystander CPR.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2021)
Article
Oncology
Yi-Ping Yang, Andy Chi-Lung Lee, Liang-Ting Lin, Yi-Wei Chen, Pin- Huang, Hsin- Ma, Yi-Chen Chen, Wen-Liang Lo, Yuan-Tzu Lan, Wen-Liang Fang, Chien-Ying Wang, Yung-Yang Liu, Po-Kuei Hsu, Wen-Chang Lin, Chung-Pin Li, Ming-Teh Chen, Chian-Shiu Chien, Mong-Lien Wang
Summary: This study presents a new therapeutic strategy for GBM tumor by targeting the protein-protein interaction between MSI1 and AGO2 with synthetic peptides. The decoy peptides identified from the C-terminus of MSI1 showed promising therapeutic efficacy in suppressing tumor growth and prolonging survival rates. These findings provide a new rationale for developing targeted therapeutics for GBM.
Article
Biochemistry & Molecular Biology
Yi-Ping Yang, Yuh-Lih Chang, Yun-Hsien Lai, Ping-Hsing Tsai, Yu-Jer Hsiao, Long Hoang Nguyen, Xue-Zhen Lim, Chang-Chi Weng, Yu-Ling Ko, Chang-Hao Yang, De-Kuang Hwang, Shih-Jen Chen, Shih-Hwa Chiou, Guang-Yuh Chiou, An-Guor Wang, Yueh Chien
Summary: In this study, researchers found differential expression of circular RNA hsa_circ_0087207 between iPSC-derived RGCs generated from LHON patients and carriers, suggesting its involvement in the pathogenesis of LHON through the miR-665 pathway. This finding provides a novel therapeutic target for LHON treatment.
Article
Materials Science, Multidisciplinary
Meng-Shiue Lee, Yueh Chien, Pai-Chi Teng, Xuan-Yang Huang, Yi-Ying Lin, Ting-Yi Lin, Shih-Jie Chou, Chian-Shiu Chien, Yu-Jer Hsiao, Yi-Ping Yang, Wensyang Hsu, Shih-Hwa Chiou
Summary: This study demonstrates that the doubly reentrant topology (DRT) surfaces have a significant antibiofouling effect that can effectively prevent viral contamination. This effect still exists even if the DRT surface is made of a hydrophilic material. By minimizing the contact area between pathogens and surfaces, fomite transmission of viruses can be effectively prevented. The unique geometric features of DRT surfaces also contribute to their excellent antibiofouling ability, which may shed light on pathogen elimination in alleviating the COVID-19 pandemic.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Cell Biology
Chih-Jung Chen, Ya-Chuan Hu, Yueh Chien, Wei-Chieh Huang, Chi-Sheng Wu, Chung-Ying Tsai, Yang-Hsiang Lin, Meng-Shiue Lee, Chian-Shiu Chien, Yi-Ping Yang, Meng-Chou Lee, Chung-Chih Tseng, Hsiang-Cheng Chi
Summary: This study reveals the correlation between the malignancy of neuroblastoma (NB) cells and the expression of Calreticulin (CALR), as well as their radioresistant and stem cell properties. Manipulating CALR expression can alter the stem cell characteristics and radioresistance of NB cells. CALR overexpression increases ROS production and secretion of proinflammatory cytokines, significantly inhibiting the growth of NB tumors, especially when combined with radiotherapy.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2023)
