Article
Fisheries
Sen Fan, Hong -Juan Shi, You -Xing Peng, Yuan-Qing Huang, Mou-Yan Jiang, Guang-Li Li, De-Shou Wang, Dong-Neng Jiang
Summary: Gonadal soma-derived factor (gsdf) is critical for sex determination and differentiation in fish, but its functions in spermatogenesis are unclear. This study found that XY gsdf/Nile tilapia can be converted to males by inhibiting estrogen synthesis, and these male fish are infertile. Transcriptome analysis revealed dysregulation of genes related to the endocrine system and azoospermia in these infertile fish. These findings provide a foundation for future research on the role of gsdf in spermatogenesis in fish.
Article
Fisheries
Chenxu Wang, Thomas D. Kocher, Baoyue Lu, Jia Xu, Deshou Wang
Summary: In this study, we successfully generated a silver-white tilapia through mutation of the hps4 gene using CRISPR/Cas9 gene editing. The mutation resulted in a significant decrease of melanophores and melanin, and a significant increase of iridophores, xanthophores, and erythrophores. This research represents the first report on the disruption of HPS4 in teleosts and the regulation of iridophore, xanthophore, and erythrophore numbers by HPS4.
Article
Fisheries
Chenxu Wang, Thomas D. Kocher, Jinzhi Wu, Peng Li, Guangyuan Liang, Baoyue Lu, Jia Xu, Xiaoke Chen, Deshou Wang
Summary: This study reveals that both mitf genes play important roles in body color formation in tilapia. Mutation of mitfa leads to light yellow body color, mutation of mitfb leads to slight hypo-pigmentation, and double mutation of both genes results in dramatic hypopigmentation. This research not only provides insights into the function of mitf in tilapia but also offers new strategies for breeding red and yellow tilapia for aquaculture.
Article
Pharmacology & Pharmacy
Xueyan Gou, Fenglin Ran, Jinru Yang, Yanrong Ma, Xin'an Wu
Summary: In this study, a Slc22a6/Slc22a8 double-knockout rat model was successfully generated using CRISPR/Cas9 technology. This model provides a useful tool for investigating the functions of OAT1/OAT3 in metabolic diseases, drug metabolism and pharmacokinetics, and OATs-mediated drug interactions.
Article
Reproductive Biology
Gyeong-Min Gim, Kyeong-Hyun Uhm, Dong-Hyeok Kwon, Min-Ji Kim, Dae-Jin Jung, Dae-Hyun Kim, Jun-Koo Yi, Jae-Jung Ha, Soo-Young Yum, Woo-Jae Son, Ji-Hyun Lee, Ji-Hyun Park, Kil-Young Song, Won-Wu Lee, Goo Jang
Summary: This study assessed the germline transmission of MSTN mutations in F0 cattle and demonstrated successful transmission of the mutations to the next generation. Additionally, the study showed the possibility of further gene editing using electroporation in embryos fertilized with the mutant sperms.
Article
Cell Biology
Takuma Komori, Shoji Hata, Akira Mabuchi, Mariya Genova, Tomoki Harada, Masamitsu Fukuyama, Takumi Chinen, Daiju Kitagawa
Summary: The advance of CRISPR/Cas9 technology has enabled us easily to generate gene knockout cell lines. However, there is growing evidence that targeted genes are not always knocked out. Here, we established a pipeline of CRISPR-del, which induces a large chromosomal deletion, to perform 'complete' gene knockout efficiently in human diploid cells.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sarithaa Sellaththurai, Sumi Jung, Myoung-Jin Kim, Kishanthini Nadarajapillai, Subothini Ganeshalingam, Joon Bum Jeong, Jehee Lee
Summary: This study investigated the role of Sting in bacterial infections by creating a knockout zebrafish model. The results showed that deficiency of Sting enhances immune responses and plays an important role in E. coli infection.
Article
Cell Biology
Bastian Brauer, Nicolas Merino-Veliz, Constanza Ahumada-Marchant, Gloria Arriagada, Fernando J. Bustos
Summary: Using CRISPR/Cas9 gene editing, researchers found that knocking out KMT2C expression in cortical neurons and the mouse brain led to decreased KMT2C levels and the development of repetitive behaviors, social deficits, and intellectual disability resembling ASD. This study sheds light on the importance of KMT2C in neurodevelopmental processes and establishes a valuable model for understanding the cellular and molecular mechanisms underlying KMT2C mutations and their relationship to Kleefstra syndrome 2 and ASD.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Entomology
Meng-Qi Zhang, Lang-Lang Gong, Ya-Qin Zhao, Yun-Feng Ma, Gui-Jun Long, Huan Guo, Xuan-Zheng Liu, J. Joe Hull, Youssef Dewer, Chao Yang, Ning-Ning Zhang, Ming He, Peng He
Summary: Direct parental CRISPR (DIPA-CRISPR) was used to edit the eye pigment gene in the white-backed planthopper. This study demonstrated the potential of DIPA-CRISPR in non-model insect species and its application in pest management.
Review
Marine & Freshwater Biology
Minghui Li, Shengfei Dai, Xingyong Liu, Hesheng Xiao, Deshou Wang
Summary: Reverse genetics approaches, especially CRISPR/Cas9, are essential for understanding complex biological processes and genetic engineering. Nile tilapia, with its XX/XY sex determination system, serves as a valuable model for studying fish sex determination and reproduction. The detailed protocol provided in this study will facilitate the application of CRISPR/Cas9 in tilapia research and aquaculture studies.
Article
Multidisciplinary Sciences
Stefanie Kurtz, Andrea Lucas-Hahn, Brigitte Schlegelberger, Gudrun Goehring, Heiner Niemann, Thomas C. Mettenleiter, Bjoern Petersen
Summary: This research explores the central role of the HMG domain in male porcine sex determination through a knockout experiment of the SRY gene with pathogenic modifications, resulting in genetically male pigs with internal female genitalia. The study could assist in solving the problem of sex preference in agriculture and establishes a more comparable large animal model for the study and development of interventions for human sex development disorders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Agriculture, Multidisciplinary
Yang Jing, Fang Mu, Xiaoxu Xing, Jiaxin Huang, Ming Lou, Haidong Xu, Bolin Ning, Yuqi Lou, Zhihui Gao, Haoyu Luo, Xiaohong Yan, Hui Li, Ning Wang
Summary: The study finds that PPAR gamma 1 and PPAR gamma 2 have differential effects on chicken adipogenesis, demonstrating distinct regulatory roles.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
W. Patrick Bewg, Scott A. Harding, Nancy L. Engle, Brajesh N. Vaidya, Ran Zhou, Jacob Reeves, Thomas W. Horn, Nirmal Joshee, Jerry W. Jenkins, Shengqiang Shu, Kerrie W. Barry, Yuko Yoshinaga, Jane Grimwood, Robert J. Schmitz, Jeremy Schmutz, Timothy J. Tschaplinski, Chung-Jui Tsai
Summary: In this study, a single gRNA was used to target conserved sequences for efficient mutagenesis of a multigene family and the recovery of specific mutants in poplar plants. Deep sequencing and PCR analyses confirmed successful editing across multiple targets with minimal off-target mutations. Additionally, the study revealed a potential new role for non-glandular trichomes in poplar plants.
Article
Multidisciplinary Sciences
Zongzhuang Wen, Haixia Zhu, Bin Wu, Aizhen Zhang, Hongxiang Wang, Yin Cheng, Hui Zhao, Jianyuan Li, Min Liu, Jiangang Gao
Summary: This study revealed the important role of CTSB in spermatogenesis and sperm maturation in mice. By generating Ctsb-/- mice using CRISPR/Cas9 technology, the researchers found that these mice had decreased sperm count, increased levels of morphologically abnormal sperm, decreased levels of progressively motile sperm, and increased levels of immotile sperm. Histological analysis showed vacuolization in the testis epithelium and loss of epididymal epithelium cells in Ctsb-/- mice. Furthermore, autophagic activity was inhibited and apoptotic activity was increased in both the testis and epididymis of Ctsb-/- mice.
Article
Dentistry, Oral Surgery & Medicine
R. Sekiguchi, M. M. Mehlferber, K. Matsumoto, S. Wang
Summary: This study presents a method for efficient gene knockout in ex vivo mouse embryonic salivary epithelial explants using CRISPR-Cas9. The researchers demonstrated high perturbation efficiency by transient transduction of lentiviruses expressing guide RNAs into Cas9-expressing salivary epithelial buds. They also developed a workflow to produce experiment-ready lentiviruses within a week. The method enables efficient perturbation of genes of interest in salivary epithelial explants, facilitating studies of specific gene functions.
JOURNAL OF DENTAL RESEARCH
(2023)
Review
Marine & Freshwater Biology
Minghui Li, Shengfei Dai, Xingyong Liu, Hesheng Xiao, Deshou Wang
Summary: Reverse genetics approaches, especially CRISPR/Cas9, are essential for understanding complex biological processes and genetic engineering. Nile tilapia, with its XX/XY sex determination system, serves as a valuable model for studying fish sex determination and reproduction. The detailed protocol provided in this study will facilitate the application of CRISPR/Cas9 in tilapia research and aquaculture studies.
Article
Biochemistry & Molecular Biology
Wenjing Tao, Luohao Xu, Lin Zhao, Zexian Zhu, Xin Wu, Qianwen Min, Deshou Wang, Qi Zhou
Summary: Researchers have reported the newly produced genomes of Nile tilapia and blue tilapia, providing detailed insights into their sex chromosome turnover mechanisms and sex-determining pathways, which are crucial for the development of genetic sex control in aquaculture. The study suggests that unequal distribution of repeat content may impact the local recombination rate, making some chromosomes more likely to become sex chromosomes.
MOLECULAR ECOLOGY RESOURCES
(2021)
Article
Fisheries
Xin Wu, Lin Zhao, Zheng Fan, Baoyue Lu, Jinlin Chen, Dejie Tan, Dongneng Jiang, Wenjing Tao, Deshou Wang
Summary: The study bred WW super-females, identified structural variations in the sex determining region of blue tilapia, designed and screened three sex-linked markers, and successfully established a genetic sex identification technique. This is significant for studying sex determination genes, understanding sex chromosome evolution, and the transition of sex determination systems.
Article
Evolutionary Biology
Chenxu Wang, Baoyue Lu, Tao Li, Guangyuan Liang, Mengmeng Xu, Xingyong Liu, Wenjing Tao, Linyan Zhou, Thomas D. Kocher, Deshou Wang
Summary: The diverse color patterns of cichlid fishes are important in mate choice and speciation. Different genes control body and eye pigmentation in Nile tilapia. Several genes, including mitf, kita/kitlga, and pmel, are significant for color patterning in tilapia, making it an excellent model system for studying vertebrate color patterning.
JOURNAL OF HEREDITY
(2021)
Article
Biochemistry & Molecular Biology
Zhisheng Ma, Jing Yang, Qingqing Zhang, Chunmei Xu, Jing Wei, Lina Sun, Deshou Wang, Wenjing Tao
Summary: miR-133b, a member of the miR-133 family, plays a crucial role in tilapia oogenesis by regulating the expression of tagln2 gene and impacting estrogen synthesis. The study validates the role of miR-133b in fish reproduction through experiments in mice, laying the foundation for further exploration of the regulatory network in fish oogenesis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Lanying Yang, Xuefeng Zhang, Shujun Liu, Chenhua Zhao, Yiyang Miao, Li Jin, Deshou Wang, Linyan Zhou
Summary: In teleost fish, the gene Cyp17a1 plays a crucial role in steroidogenesis, sex determination, and fertility. Mutations in the cyp17a1 gene in Nile tilapia led to female-to-male sex reversal and deficiencies in hormone production, impacting both the sex determination and reproductive processes in fish. Overall, Cyp17a1 is found to be essential for E2 production in female sex determination and differentiation, as well as for T and 11-KT production in promoting spermatogenesis and fertility in male fish.
Review
Endocrinology & Metabolism
Linyan Zhou, Minghui Li, Deshou Wang
Summary: The involvement of sex steroids in sex determination and differentiation in non-mammalian vertebrates, especially in fish, is relatively conserved. Advances in genome sequencing and editing have led to significant progress in understanding steroidogenic pathways and hormonal regulation of sex determination in fish. Gene editing studies challenge traditional views on the roles of estrogen and androgen in ovarian and testicular development, and highlight the critical role of estrogen in female sex determination.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lu Li, You Wu, Chenhua Zhao, Yiyang Miao, Jing Cai, Lingyun Song, Jing Wei, Tapas Chakraborty, Limin Wu, Deshou Wang, Linyan Zhou
Summary: The StAR2 gene plays a critical role in testicular development, spermatogenesis, and spermiation in tilapia by regulating androgen production. However, it may not be essential and could be compensated for in the absence of StAR2.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Genetics & Heredity
Xingyong Liu, Shengfei Dai, Jiahong Wu, Xueyan Wei, Xin Zhou, Mimi Chen, Dejie Tan, Deyong Pu, Minghui Li, Deshou Wang
Summary: The study analyzed the expression and functions of amh duplicates in Nile tilapia. It was found that amhy is expressed earlier and at a higher level during sex determination, and it determines sex by repressing the transcription of cyp19a1a. Mutations in amhy lead to sex reversal and altered germ cell proliferation.
Article
Evolutionary Biology
Chenxu Wang, Jia Xu, Thomas D. Kocher, Minghui Li, Deshou Wang
Summary: In this study, researchers identified two pmel genes in Nile tilapia and demonstrated their importance in pigmentation. Homozygous mutation of pmela resulted in a yellowish body color with weak vertical bars, while mutation of pmelb had a milder effect. Double mutation of both genes led to a decrease in melanophores and an increase in xanthophores, resulting in a golden body color. The findings provide new strategies for breeding golden tilapia and offer a new model for studying pmel function in vertebrates.
JOURNAL OF HEREDITY
(2022)
Article
Biology
Shuqing Zheng, Wenjing Tao, Haowen Yang, Thomas D. Kocher, Zhijian Wang, Zuogang Peng, Li Jin, Deyong Pu, Yaoguang Zhang, Deshou Wang
Summary: Teleosts are important models for studying sex chromosomes and sex-determining genes. In this study, a high-contiguity chromosome-level genome assembly on a YY southern catfish was generated using Nanopore and Hi-C technologies. The study identified a candidate sex-determining gene in the sex-determining region and confirmed the role of amhr2y in male sex determination.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Chunmei Xu, Miao Yu, Qingqing Zhang, Zhisheng Ma, Kang Du, Huiqin You, Jing Wei, Deshou Wang, Wenjing Tao
Summary: In this study, the BRD gene family was identified and characterized in Nile tilapia, revealing sexually dimorphic expression patterns and their critical role in spermatogenesis. The findings provide insights into the understanding of sex differentiation and gonadal development in teleosts.
Correction
Biology
Shuqing Zheng, Wenjing Tao, Haowen Yang, Thomas D. Kocher, Zhijian Wang, Zuogang Peng, Li Jin, Deyong Pu, Yaoguang Zhang, Deshou Wang
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xingyong Liu, Li Zhou, Wenbo Li, Jiahong Wu, Deshou Wang
Summary: In this study, it was found that GATA2 plays a critical role in lens morphogenesis in Nile tilapia. Mutations in the gata2a gene led to degeneration and apoptosis of lens fiber cells, causing progressive microphthalmia and blindness in adulthood. Transcriptome analysis revealed significant changes in the expression levels of genes encoding crystallin and genes involved in visual perception and metal ion binding after gata2a mutation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Zeming Zhang, Jie Wang, Jianeng Li, Xiang Liu, Lei Liu, Changle Zhao, Wenjing Tao, Deshou Wang, Jing Wei
Summary: In this study, an integrated CRISPR/Cas9 system was established for gene knock-out and knock-in at a specific site in medaka. The system is simple, cost-effective, and efficient. The results showed that pCas9-mU6sgRNA driven by endogenous U6 promoter achieved high gene editing efficiency in medaka cultured cells, but not by exogenous U6 promoters. This study provides insights into other fish genome editing and promotes gene functional analysis.
