Article
Cell Biology
Lea Flippe, Anne Gaignerie, Celine Serazin, Olivier Baron, Xavier Saulquin, Ignacio Anegon, Laurent David, Carole Guillonneau
Summary: Immunotherapy using primary T cells has greatly improved medical care in recent years, but limitations such as challenging cell genome editing and lack of standardized products have restricted its clinical use. The use of T cells derived from human pluripotent stem cells offers advantages of genetic modification and standardized allogeneic products. However, more research is needed to fully understand the feasibility and functionality of these T cells before clinical applications. This study successfully generated T cells from induced pluripotent stem cells, demonstrating the potential for therapeutic use and treatment of various diseases.
Article
Genetics & Heredity
Takahiro Sano, Koki Ueda, Keiji Minakawa, Tsutomu Mori, Yuko Hashimoto, Haruhiko Koseki, Yasuchika Takeishi, Kazuhiko Ikeda, Takayuki Ikezoe
Summary: This study generated Uhrf2(-/-) mice to investigate the role of UHRF2 deletion in hematopoiesis. The results showed that the absence of UHRF2 did not affect blood counts, bone marrow findings, and spleen weights. However, the proportions of Uhrf2(-/-) cells were decreased compared to Uhrf2(+/+) cells in competitive repopulation assays, indicating a potential regulatory role of UHRF2 in hematopoiesis.
Review
Immunology
Lindsey B. B. Crawford
Summary: Human betaherpesviruses, including HCMV, HHV-6a, HHV-6b, and HHV-7, infect and establish latency in CD34+ HPCs. The interactions between these viruses and host HPCs regulate viral lifecycle and latency. Precise manipulation of host and viral factors contribute to viral genome maintenance, increased host cell survival, and manipulation of the cellular environment. This review highlights the complexities of betaherpesvirus latency and HPC virus-host interactions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chen-Yuan Kao, Jinlin Jiang, Will Thompson, Eleftherios T. Papoutsakis
Summary: Megakaryocyte-derived submicron size microparticles (MkMPs) induce the megakaryocytic differentiation of hematopoietic stem/progenitor cells (HSPCs) through the combined effects of miR-486-5p and miR-22-3p, as well as through JNK and PI3K/Akt/mTOR signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Anatomy & Morphology
Minami Ezawa, Fumika Kouno, Hideo Kubo, Tetsushi Sakuma, Takashi Yamamoto, Tsutomu Kinoshita
Summary: In Xenopus development, Pou5f3.3 plays a crucial role in establishing and maintaining hematopoietic progenitor cells, contributing to the regulation of peripheral blood cell homeostasis. Transplantation experiments demonstrate the migration of Pou5f3.3-positive cells from the hematopoietic dorsal-lateral plate to the liver and bone through the bloodstream, highlighting its significance in hematopoiesis.
Article
Developmental Biology
Carrie M. Spratford, Lauren M. Goins, Fangtao Chi, Juliet R. Girard, Savannah N. Macias, Vivien W. Ho, Utpal Banerjee
Summary: Genetic and genomic analysis in Drosophila suggests that hematopoietic progenitors likely transition into terminal fates via intermediate progenitors with unique characteristics. These intermediate progenitors (IPs) play a crucial role in blood cell development, remaining multipotent and dynamically controlling the fate of neighboring cells. The Ras pathway regulates the number of IP cells and promotes their transition into differentiating cells.
Article
Immunology
Theodore T. Ho, Paul Dellorusso, Evgenia V. Verovskaya, Sietske T. Bakker, Johanna Flach, Lucas K. Smith, Patrick B. Ventura, Olivia M. Lansinger, Aurelie Herault, Si Yi Zhang, Yoon-A Kang, Carl A. Mitchell, Saul A. Villeda, Emmanuelle Passegue
Summary: The study indicated that despite the rejuvenating effects of young blood on aged tissues, the aged blood system and old hematopoietic stem cells (HSCs) are not significantly affected by exposure to young blood. Old HSC function is not improved by exercise, calorie restriction, or exposure to young niches, while young HSCs are resistant to systemic proaging conditions.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biology
Wei Zou, Juanjuan Xing, Shijie Zou, Mei Jiang, Xinping Chen, Qi Chen, Daozheng Liu, Xiangcheng Zhang, Xin Fu
Summary: The study found that HIV-1 (LAI) Nef interferes with the development of HSPCs into myeloid-erythroid lineage cells, thus disrupting hematopoiesis. Therapeutics targeting Nef may hold promise in correcting HIV-1 associated hematopoietic abnormalities.
Article
Multidisciplinary Sciences
Guanghua Wan, Sebastian Medina, Haikun Zhang, Rong Pan, Xixi Zhou, Alicia M. Bolt, Li Luo, Scott W. Burchiel, Ke Jian Liu
Summary: Low-dose arsenic exposure inhibits erythroid differentiation of human hematopoietic progenitor cells, leading to impaired human erythropoiesis and affecting various stages of red blood cell formation.
SCIENTIFIC REPORTS
(2021)
Article
Cell & Tissue Engineering
Kenji Kitajima, Minako Shingai, Hikaru Ando, Mako Hamasaki, Takahiko Hara
Summary: Researchers developed a novel method for hematopoietic differentiation of human-induced pluripotent stem cells to address the issue of low production of CD38(+) hematopoietic progenitor cells. They found that the lack of FLT3 expression was responsible for this problem and solved it by adding interferon-gamma.
Article
Cell & Tissue Engineering
Hector Huerga Encabo, Iker Valle Aramburu, Manuel Garcia-Albornoz, Marion Piganeau, Henry Wood, Anna Song, Alessandra Ferrelli, Aneesh Sharma, Carlos M. Minutti, Marie-Charlotte Domart, Despoina Papazoglou, Kristian Gurashi, Miriam Llorian Sopena, Robert Goldstone, Todd Fallesen, Qian Wang, Linda Ariza-McNaughton, Daniel H. Wiseman, Kiran Batta, Rajeev Gupta, Venizelos Papayannopoulos, Dominique Bonnet
Summary: Somatic mutations in hematopoietic stem cells (HSCs) can lead to clonal hematopoiesis (CH) and the production of mutated immune cells, increasing the risk of leukemia and inflammatory diseases. Through genetic engineering and transplantation, researchers found that loss of the TET2 gene in human HSCs resulted in abnormal development and function of neutrophils, leading to heightened inflammatory responses and compact chromatin structure. These findings provide insights into detecting TET2-CH and preventing CH-associated pathologies mediated by neutrophil extracellular traps (NET).
Article
Cell Biology
Diana Sa da Bandeira, Alastair Morris Kilpatrick, Madalena Marques, Mario Gomez-Salazar, Telma Ventura, Zaniah Nashira Gonzalez, Dorota Stefancova, Fiona Rossi, Matthieu Vermeren, Chris Sebastiaan Vink, Mariana Beltran, Neil Cowan Henderson, Bongnam Jung, Reinier van der Linden, Harmen Jan George van de Werken, Wilfred F. J. van Ijcken, Christer Betsholtz, Stuart John Forbes, Henar Cuervo, Mihaela Crisan
Summary: This study reveals the expression of PDGFRβ in distinct perivascular stromal cell layers in mice and shows that its deletion impairs hematopoiesis. The study also demonstrates that PDGFRβ(+) cells play a dual role in murine hematopoiesis, acting as support for HSPCs in the aortic niche and giving rise to a subset of HSPCs that persist into adulthood.
Review
Hematology
Vincenzo Calvanese, Hanna K. A. Mikkola
Summary: Developmental hematopoiesis is a complex process involving the generation of differentiated blood cells and the establishment of a pool of undifferentiated hematopoietic stem cells (HSCs) for postnatal life. Recent single-cell studies have identified rare human HSCs and traced their origin to a unique type of arterial endothelium in the aorta-gonad-mesonephros region. These studies provide new insights into HSC generation and may inform efforts to generate HSCs from pluripotent stem cells in vitro.
Article
Cell & Tissue Engineering
Paul Jaeger, Stefanie Geyh, Soeren Twarock, Ron-Patrick Cadeddu, Pablo Rabes, Annemarie Koch, Uwe Maus, Tobias Hesper, Christoph Zilkens, Christina Rautenberg, Felix Bormann, Karl Koehrer, Patrick Petzsch, Dagmar Wieczorek, Beate Betz, Harald Surowy, Barbara Hildebrandt, Ulrich Germing, Guido Kobbe, Rainer Haas, Thomas Schroeder
Summary: Studies suggest that leukemic cells induce functional inhibition of healthy CD34+ HSPC, at least in part, through pathways like TGF beta 1, indicating that blocking this pathway may improve hematopoiesis in AML patients.
Article
Cell Biology
Manon Boulet, Yoan Renaud, Francois Lapraz, Billel Benmimoun, Laurence Vandel, Lucas Waltzer
Summary: The study reveals that the hematopoietic system of adult fruit flies is mainly composed of a few distinct mature blood cell types, which some of them are derived from a specialized population of cells present in the larval lymph gland. These cells, normally quiescent, can differentiate and proliferate in response to bacterial infection.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Kiminori Terui, Tsutomu Toki, Takashi Taga, Shotaro Iwamoto, Takako Miyamura, Daisuke Hasegawa, Hiroshi Moritake, Asahito Hama, Kentaro Nakashima, Rika Kanezaki, Ko Kudo, Akiko M. Saito, Keizo Horibe, Souichi Adachi, Daisuke Tomizawa, Etsuro Ito
GENES CHROMOSOMES & CANCER
(2020)
Article
Oncology
Natsuki Takahashi, Ko Kudo, Miyuki Tanaka, Naoya Kumagai, Tomohiko Sato, Takuya Kamio, Shinya Sasaki, Kiminori Terui, Akira Kurose, Ryu Yanagisawa, Yozo Nakazawa, Etsuro Ito
JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY
(2020)
Article
Rheumatology
Koji Hirono, Tadaatsu Imaizumi, Tomomi Aizawa, Shojiro Watanabe, Koji Tsugawa, Toshihiro Shiratori, Shogo Kawaguchi, Kazuhiko Seya, Tomoh Matsumiya, Etsuro Ito, Hiroshi Tanaka
MODERN RHEUMATOLOGY
(2020)
Letter
Oncology
Yoshihiro Takahashi, Kiminori Terui, Yoshiaki Chinen, Satoru Tandai, Ko Kudo, Shinya Sasaki, Chikako Tono, Tomohiko Taki, Etsuro Ito
PEDIATRIC BLOOD & CANCER
(2020)
Letter
Hematology
Yoko Nishinaka-Arai, Akira Niwa, Shiori Matsuo, Yasuhiro Kazuki, Yuwna Yakura, Takehiko Hiroma, Tsutomu Toki, Tetsushi Sakuma, Takashi Yamamoto, Etsuro Ito, Mitsuo Oshimura, Tatsutoshi Nakahata, Megumu K. Saito
Correction
Hematology
Minako Mori, Asuka Hira, Kenichi Yoshida, Hideki Muramatsu, Yusuke Okuno, Yuichi Shiraishi, Michiko Anmae, Jun Yasuda, Shu Tadaka, Kengo Kinoshita, Tomoo Osumi, Yasushi Noguchi, Souichi Adachi, Ryoji Kobayashi, Hiroshi Kawabata, Kohsuke Imai, Tomohiro Morio, Kazuo Tamura, Akifumi Takaori-Kondo, Masayuki Yamamoto, Satoru Miyano, Seiji Kojima, Etsuro Ito, Seishi Ogawa, Keitaro Matsuo, Hiromasa Yabe, Miharu Yabe, Minoru Takata
Article
Hematology
Kazuki Terada, Koichi Miyake, Hiroki Yamaguchi, Noriko Miyake, Keiichiro Yamanaka, Seiji Kojima, Etsuro Ito, Koiti Inokuchi, Takashi Okada
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2020)
Article
Biophysics
Shun Koyamaishi, Takuya Kamio, Akie Kobayashi, Tomohiko Sato, Ko Kudo, Shinya Sasaki, Rika Kanezaki, Daiichiro Hasegawa, Hideki Muramatsu, Yoshiyuki Takahashi, Yoji Sasahara, Hidefumi Hiramatsu, Harumi Kakuda, Miyuki Tanaka, Masataka Ishimura, Masanori Nishi, Akira Ishiguro, Hiromasa Yabe, Takeo Sarashina, Masaki Yamamoto, Yuki Yuza, Nobuyuki Hyakuna, Kenichi Yoshida, Hitoshi Kanno, Shouichi Ohga, Akira Ohara, Seiji Kojima, Satoru Miyano, Seishi Ogawa, Tsutomu Toki, Kiminori Terui, Etsuro Ito
Summary: This study retrospectively compared the outcomes of DBA patients undergoing HSCT with MAC or RIC conditioning regimens. All 27 patients who underwent HSCT successfully engrafted, with a 3-year overall survival rate of 95.2% and a failure-free survival rate of 88.4%, showing no significant differences between MAC and RIC regimens.
BONE MARROW TRANSPLANTATION
(2021)
Article
Hematology
Anfeng Mu, Asuka Hira, Akira Niwa, Mitsujiro Osawa, Kenichi Yoshida, Minako Mori, Yusuke Okamoto, Kazuko Inoue, Keita Kondo, Masato T. Kanemaki, Tomonari Matsuda, Etsuro Ito, Seiji Kojima, Tatsutoshi Nakahata, Seishi Ogawa, Keigo Tanaka, Keitaro Matsuo, Megumu K. Saito, Minoru Takata
Summary: A novel Fanconi anemia-like inherited bone marrow failure syndrome has been discovered in Japanese children and is associated with variants in the ADH5 and ALDH2 genes. ADH5 is identified as the primary defense against formaldehyde, with ALDH2 providing a backup mechanism. Patient-derived fibroblasts displayed normal levels of DNA repair, whereas disease model iPSCs showed defective cell expansion during hematopoietic differentiation in vitro, which could be partially reversed by treatment with a new small molecule called C1.
Article
Biochemistry & Molecular Biology
Felix A. Dingler, Meng Wang, Anfeng Mu, Christopher L. Millington, Nina Oberbeck, Sam Watcham, Lucas B. Pontel, Ashley N. Kamimae-Lanning, Frederic Langevin, Camille Nadler, Rebecca L. Cordell, Paul S. Monks, Rui Yu, Nicola K. Wilson, Asuka Hira, Kenichi Yoshida, Minako Mori, Yusuke Okamoto, Yusuke Okuno, Hideki Muramatsu, Yuichi Shiraishi, Masayuki Kobayashi, Toshinori Moriguchi, Tomoo Osumi, Motohiro Kato, Satoru Miyano, Etsuro Ito, Seiji Kojima, Hiromasa Yabe, Miharu Yabe, Keitaro Matsuo, Seishi Ogawa, Berthold Goettgens, Michael R. G. Hodskinson, Minoru Takata, Ketan J. Patel
Letter
Hematology
Yoichi Tanaka, Allen Eng Juh Yeoh, Takaya Moriyama, Chi-Kong Li, Ko Kudo, Yuki Arakawa, Jassada Buaboonnam, Hui Zhang, Hsi-Che Liu, Hany Ariffin, Zhiwei Chen, Shirley K. Y. Kham, Rina Nishii, Daisuke Hasegawa, Junya Fujimura, Dai Keino, Kensuke Kondoh, Atsushi Sato, Takahiro Ueda, Masaki Yamamoto, Yuichi Taneyama, Moeko Hino, Masatoshi Takagi, Akira Ohara, Etsuro Ito, Katsuyoshi Koh, Hiroki Hori, Atsushi Manabe, Jun J. Yang, Motohiro Kato
Article
Oncology
Takashi Taga, Shiro Tanaka, Daisuke Hasegawa, Kiminori Terui, Tsutomu Toki, Shotaro Iwamoto, Hidefumi Hiramatsu, Takako Miyamura, Yoshiko Hashii, Hiroshi Moritake, Hideki Nakayama, Hiroyuki Takahashi, Akira Shimada, Tomohiko Taki, Etsuro Ito, Asahito Hama, Masafumi Ito, Katsuyoshi Koh, Daiichiro Hasegawa, Akiko M. Saito, Souichi Adachi, Daisuke Tomizawa
Summary: In patients with ML-DS, detection of MRD after induction therapy, either by FCM or GATA1, was significantly associated with three-year event-free survival and overall survival rates. Positive MRD results were predictive of a higher risk of relapse in ML-DS patients.
Article
Oncology
Genki Yamato, Takao Deguchi, Kiminori Terui, Tsutomu Toki, Tomoyuki Watanabe, Takahiro Imaizumi, Asahito Hama, Shotaro Iwamoto, Daisuke Hasegawa, Takahiro Ueda, Tomoko Yokosuka, Shiro Tanaka, Ryu Yanagisawa, Katsuyoshi Koh, Akiko M. Saito, Keizo Horibe, Yasuhide Hayashi, Souichi Adachi, Shuki Mizutani, Takashi Taga, Etsuro Ito, Kenichiro Watanabe, Hideki Muramatsu
Article
Oncology
Satoru Monzen, Tatsuya Ueno, Mitsuru Chiba, Yuki Morino, Yasushi Mariya, Andrzej Wojcik, Lovisa Lundholm
Summary: Changes in extracellular microRNAs may serve as biomarkers for radiation-induced damage to human colorectal cancer cells. Specific microRNAs showed a significantly positive correlation with micronuclei frequency in a dose-dependent manner. These findings suggest that these specific microRNAs could be potential markers for cell damage and radiotherapy response in colorectal cancer, but further validation is needed in serum samples from patients undergoing radiotherapy.
MOLECULAR AND CLINICAL ONCOLOGY
(2022)
Article
Gastroenterology & Hepatology
Kazuya Kimura, Kazuhiro Shimazu, Tsutomu Toki, Momoko Misawa, Koji Fukuda, Taichi Yoshida, Daiki Taguchi, Sho Fukuda, Katunori Iijima, Naoto Takahashi, Etsuro Ito, Hiroshi Nanjyo, Hiroyuki Shibata
CLINICAL JOURNAL OF GASTROENTEROLOGY
(2020)
