Article
Chemistry, Multidisciplinary
Zhen Wang, Liangqi Ouyang, Hailong Li, Lars Wagberg, Mahiar Max Hamedi
Summary: The PEO/polyacrylic acid composite thin films prepared using layer-by-layer self-assembly exhibit high Li+ conductivity and strength, making them ideal for various applications such as separators for Li-ion batteries and ion-conducting substrates for flexible electrochemical devices.
Article
Nanoscience & Nanotechnology
Ting Zhao, Zhongrui Wang, Yang Yang, Kun Liu, Xu Wang
Summary: This study presents a novel strategy for achieving cyclic and waste-free nonequilibrium assembly and disassembly of macroscopic hydrogels, utilizing an ionic strength-mediated approach. The strategy involves using ammonium carbonate as a chemical fuel to temporally regulate the attractions between oppositely charged hydrogels. This concept holds promise for creating macroscopic and microscopic nonequilibrium systems and self-adaptive materials.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Microbiology
Jiuxia Yang, Lu Ge, Bo Song, Zhongqiang Ma, Xiaotian Yang, Bo Wang, Yixin Dai, Haijin Xu, Mingqiang Qiao
Summary: This study identified a new hydrophobin gene (hgfII) from Grifola frondosa with a higher transcriptional level than the existing hydrophobin gene (hgfI). Heterologous expression of hgfII was successfully achieved in Pichia pastoris. The results showed that HGFII could self-assemble into a protein film at the air-solid interface and exhibited stronger surface hydrophobicity and better emulsification capacity compared to HGFI.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Agniva Dutta, Gleb Vasilyev, Eyal Zussman
Summary: This study presents the fabrication of a polymer-protein hydrogel composed of polymethacrylamide (PMAAm) and bovine serum albumin (BSA). BSA acts as a cross-linker of polymer chains due to its specific interactions between corresponding functional groups. The hydrogel with optimized composition and preparation conditions demonstrated excellent mechanical properties, including fatigue resistance and the ability to restore damaged structures.
Article
Polymer Science
Antiopi Vardaxi, Stergios Pispas
Summary: This work presents the synthesis and stimuli-responsive self-assembly behavior of novel double-hydrophilic poly(2-(dimethylamino)ethyl methacrylate-co-(oligo ethylene glycol)methacrylate) random copolymers and their chemically modified derivatives. The synthesis was successfully conducted through RAFT polymerization and further followed by the quaternization with methyl iodide, 1-iodohexane, and 1-iodododecane. The polymers exhibited intramolecular self-folding and intermolecular self-assembly upon exposure to different pHs and temperatures.
Article
Biotechnology & Applied Microbiology
Kathleen L. Vergunst, Calem Kenward, David N. Langelaan
Summary: Hydrophobins are small proteins secreted by fungi that accumulate at interfaces, modify surface hydrophobicity, and self-assemble into large amyloid-like structures. This study presents the atomic resolution structure of hydrophobin SLH4 and compares its properties with another hydrophobin, SC16. Despite having similar structures, SLH4 and SC16 exhibit distinct rodlet morphology, self-assembly propensity, and preferred assembly conditions. Analysis suggests that conformational changes in residues in the first intercysteine loop play a role in rodlet formation. This research provides insights into the structure and function of hydrophobins and their potential applications as surface modifiers.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Alan Puckowski, Weronika Cwiek, Katarzyna Mioduszewska, Piotr Stepnowski, Anna Bialk-Bielinska
Summary: The study found that microplastics have a complex nature of sorption on pharmaceuticals, including both hydrophobic and electrostatic interactions. The ionic strength of the medium was identified as a significant factor influencing the sorption potential in common conditions of the natural environment.
Article
Chemistry, Inorganic & Nuclear
Weiming Huang, Zhonghao Zhang, Yinglan Wu, Wanmin Chen, David A. Rotsch, Louis Messerle, Zhiping Zheng
Summary: In this study, a series of polynuclear lanthanide complexes were prepared by using halide ions as templates, showing potential for magnetic properties research and applications.
INORGANIC CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Multidisciplinary
Jianhua Wang, Yuhui Wei, Ping Zhang, Yue Wang, Qinglin Xia, Xiaoguo Liu, Shihua Luo, Jiye Shi, Jun Hu, Chunhai Fan, Bin Li, Lihua Wang, Xingfei Zhou, Jiang Li
Summary: In this study, the folding processes of several multidomain DNA origami structures were visualized using atomic force microscopy under ambient annealing conditions in solution, revealing the coexistence of diverse transitional structures that might result in the same prescribed products. Based on experimental observations and energy landscape simulations, the heterogeneity of the folding pathways of multidomain DNA origami structures was proposed.
Article
Chemistry, Physical
Kathryn G. Wilcox, Grace M. Kemerer, Svetlana Morozova
Summary: In this study, the relationship between collagen triple helical structure and fibril formation in different ionic environments was investigated. The results show that increasing ionic strength affects the elastic properties of collagen triple helices and the diameter of fibrils. Understanding the mechanism of collagen triple helical structure and fibril assembly can provide insights into biomacromolecule self-assembly.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Yixin Peng, Pengzhao Xu, Shifang Duan, Jiayu Liu, Jeffrey Lawrence Moran, Wei Wang
Summary: This article proposes and experimentally confirms the rule that the speeds of self-diffusiophoretic colloidal motors scale inversely to their population density, while self-electrophoretic motors do not follow this rule. These rules are simple, powerful, and applicable to various colloidal motors.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Environmental Sciences
Jingyi Ling, Siheng Zheng, Feng Sheng, Hao Wu, Zhanghao Chen, Cheng Gu, Xin Jin
Summary: This study systematically investigated the effects of pH, inorganic anions, and ionic strength on the formation of brown carbon (BrC) in aerosols. The results show that these factors strongly influence BrC formation by affecting the oxidation activity and complexation capability of different Fe(III) species. Among all Fe(III) species, FeOH2+ is identified as the most reactive in the formation of BrC. Additionally, the aggregation process of BrC is affected by different anions, with SO42- exhibiting the highest efficiency in inducing aggregation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Nanoscience & Nanotechnology
Shuo Yang, Wenyan Liu, Yuwei Zhang, Risheng Wang
Summary: In this study, a new strategy utilizing DNA origami tiles and cation-controlled surface diffusion was employed to assemble gold nanorods into 1D and 2D arrays, with a novel pattern transfer method introduced for further manipulation. The results demonstrate the potential application of this approach in constructing complex superstructures with high yield and minor structural damage.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Environmental
Shuyan Xu, Caiqin Wang, Pengfeng Zhu, Daoyong Zhang, Xiangliang Pan
Summary: The environmental behavior and ecotoxicity of microplastics (MPs) are significantly affected by the self-assembly of microbial extracellular polymeric substances (EPS) on their surface. This study investigated the nanoscale heterogeneity of EPS self-assembly onto fresh and aged polypropylene (PP) MPs using atomic-force-microscopy-infrared-spectroscopy (AFM-IR). The results showed that natural aging caused physical and chemical fragmentation of MPs at the nanoscale. The self-assembly of EPS on MPs was aging-dependent, with polysaccharides assembling faster than proteins.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Physical
Eli Sutter, Bo Zhang, Peter Sutter
Summary: Directed self-assembly using pH as a stimulus can modify the interparticle interaction between Au nanorods conjugated with single-stranded DNA, allowing for on-demand self-assembly. The study demonstrates the versatile building blocks of ssDNA-conjugated anisotropic nanostructures with stimuli-programmable interactions.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Engineering, Electrical & Electronic
An Hung Nguyen, Thomas Guillemette, Andrew J. Lambert, Mark R. Pickering, Matthew A. Garratt
JOURNAL OF ELECTRONIC IMAGING
(2017)
Article
Mycology
Stephane Cuenot, Jean-Philippe Bouchara
Article
Mycology
Victoire Pateau, Bienvenue Razafimandimby, Patrick Vandeputte, Christopher R. Thornton, Thomas Guillemette, Jean-Philippe Bouchara, Sandrine Giraud
Article
Chemistry, Applied
Agata Zykwinska, Melanie Marquis, Corinne Sinquin, Laetitia Marchand, Sylvia Colliec-Jouault, Stephane Cuenot
CARBOHYDRATE POLYMERS
(2018)
Article
Chemistry, Medicinal
Agata Zykwinska, Melanie Marquis, Mathilde Godin, Laetitia Marchand, Corinne Sinquin, Catherine Garnier, Camille Jonchere, Claire Chedeville, Catherine Le Visage, Jerome Guicheux, Sylvia Colliec-Jouault, Stephane Cuenot
Article
Chemistry, Analytical
Karl Norris, Oksana I. Mishukova, Agata Zykwinska, Sylvia Colliec-Jouault, Corinne Sinquin, Andrei Koptioug, Stephane Cuenot, Jemma G. Kerns, Maria A. Surmeneva, Roman A. Surmenev, Timothy E. L. Douglas
Article
Chemistry, Physical
Stephane Hoang, Simon Olivier, Stephane Cuenot, Agnes Montillet, Jerome Bellettre, Elena Ishow
Article
Microbiology
Nicolas Valette, Julien Renou, Alexis Boutilliat, Antonio Jose Fernandez-Gonzalez, Valerie Gautier, Philippe Silar, Christophe Guyeux, Jean-Claude Charr, Stephane Cuenot, Christophe Rose, Eric Gelhaye, Melanie Morel-Rouhier
Summary: Secreted proteins play a key role in fungal physiology and protection against external stressors, with Oak stress-induced protein 1 (OSIP1) identified as having a crucial function in fungal cell wall integrity and response to stressors. OSIP1 is involved in fungal resistance to both drugs and extractives, highlighting its importance in fungal defense mechanisms.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Cindy Staerck, Hajar Yaakoub, Patrick Vandeputte, Julie Tabiasco, Charlotte Godon, Amandine Gastebois, Sandrine Giraud, Thomas Guillemette, Alphonse Calenda, Yves Delneste, Maxime Fleury, Jean-Philippe Bouchara
Summary: The mutation of the SODD gene in S. apiospermum fungi increases susceptibility to oxidizing agents and antifungal drugs, as well as intracellular killing by human macrophages. Furthermore, the study suggests a potential indirect role of SodD in the synthesis or assembly of cell wall components.
Article
Chemistry, Applied
Olga Makshakova, Agata Zykwinska, Stephane Cuenot, Sylvia Colliec-Jouault, Serge Perez
Summary: Infernan, an exopolysaccharide from the bacterial strain GY785, consists of a complex repeating unit established on a double-layer of sidechains. Molecular dynamics analysis revealed that the structure of Infernan exhibits extended two-fold and five-fold helical structures with unique chain-chain interactions. The presence of negatively charged cavities allows for Ca2+ binding in a heterotypic binding mode, with the five-fold helices being the most favorable for interactions.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Perrine Gelebart, Stephane Cuenot, Corinne Sinquin, Boris Halgand, Sophie Sourice, Catherine Le Visage, Jerome Guicheux, Sylvia Colliec-Jouault, Agata Zykwinska
Summary: This study investigated the ability of a bacterial exopolysaccharide called Infernan to bind and release BMP-2, and designed two delivery systems using a microfluidic approach. The results show that the release kinetics and bioactivity of BMP-2 are highly influenced by the ionic strength.
CARBOHYDRATE POLYMERS
(2022)
Article
Plant Sciences
Mailen Ortega-Cuadros, Tiago Lodi De Souza, Romain Berruyer, Sophie Aligon, Sandra Pelletier, Jean-Pierre Renou, Tatiana Arias, Claire Campion, Thomas Guillemette, Jerome Verdier, Philippe Grappin
Summary: The transmission of seed-borne pathogens during seed germination is a major cause of crop diseases. However, the immune responses of germinating seeds to biotic invaders are poorly understood. This study used the Arabidopsis thaliana/Alternaria brassicicola patho-system to investigate the transcriptional responses of germinating seeds and young seedlings to infection by the necrotrophic fungus. The results revealed non-canonical immune responses in early germinating seeds compared to early seedling establishment stages.
Article
Chemistry, Applied
Agata Zykwinska, Olga Makshakova, Perrine Gelebart, Corinne Sinquin, Nicolas Stephant, Sylvia Colliec-Jouault, Serge Perez, Stephane Cuenot
Summary: This study investigates the structure-function relationship of a bacterial exopolysaccharide called infernan, offering important insights for the development of hydrogels.
CARBOHYDRATE POLYMERS
(2022)
Article
Engineering, Biomedical
Stephane Cuenot, Perrine Gelebart, Corinne Sinquin, Sylvia Colliec-Jouault, Agata Zykwinska
Summary: In the field of tissue engineering, hydrogels with similar biological and mechanical properties as the extracellular matrix are being developed. The mechanical properties of these hydrogels, including their relaxation behavior and time-dependent responses, are complex. This study used Atomic Force Microscopy (AFM) to investigate the relaxation mechanisms of chemically and physically crosslinked hydrogels. Different relaxation mechanisms were observed depending on the type of hydrogel network crosslinks. The study also found that the ionic strength affects the long-term elastic modulus and relaxation times of physical hydrogels. These findings suggest that physical hydrogels with specific time-dependent mechanical properties can be optimized for cellular responses.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Elodie Belmas, Martial Briand, Anthony Kwasiborski, Justine Colou, Guillaume N'Guyen, Beatrice Iacomi, Philippe Grappin, Claire Campion, Philippe Simoneau, Matthieu Barret, Thomas Guillemette
GENOME ANNOUNCEMENTS
(2018)
Article
Biochemistry & Molecular Biology
Trang Van Tran, Hoa Nguyen, Luyen Vu, ChangWoo Lee
Summary: Glutaredoxin 3 (Grx3) is a redox protein that maintains structural integrity and glutathione (GSH) binding capabilities across different temperatures. This study investigates the roles of specific bonds in Grx3's structure and function, and how psychrophilic Grx3 variants adapt to cold environments. The highly conserved Arg51-Asp69 salt bridge and Gln56-His63 hydrogen bond are crucial for stabilizing the structure and catalytic activity of Grx3. Psychrophilic variants of Grx3 have adapted to cold environments by reducing GSH binding and increasing structural flexibility.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Amanda Lais de Souza Coto, Arthur Alexandre Pereira, Sabrina Dorta Oliveira, Milene Nobrega de Oliveira Moritz, Arthur Moraes Franco da Rocha, Paulo Roberto Dores-Silva, Noeli Soares Melo da Silva, Ana Rita de Araujo Nogueira, Lisandra Marques Gava, Thiago Vagas Seraphim, Julio Cesar Borges
Summary: J-domain proteins form a large molecular chaperone family involved in proteostasis processes, with hDjC20 playing a vital role in mitochondria and being heavily influenced by the presence of Zn+2.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Meiling Zhang, Jiaxiang Zhang, Yan Liang, Shicheng Tian, Shuyang Xie, Tong Zhou, Qin Wang
Summary: This study determined the crystal structures of RGLG2 VWA domain in Arabidopsis thaliana, revealing that Ca2+ ions act as regulators and affect the conformational change of RGLG2-VWA domain.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Alexandra Bork, Sander H. J. Smits, Lutz Schmitt
Summary: This study reveals the structure and calcium ion binding properties of CBL1 protein, and proposes a binding model of CBL1 for Ca2+. Additionally, it provides preliminary insights into the formation of the dimer interface of CBL1.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Evgeniia V. Leisi, Andrey V. Moiseenko, Sofia S. Kudryavtseva, Denis V. Pozdyshev, Vladimir I. Muronetz, Lidia P. Kurochkina
Summary: The pathogenesis of prion diseases involves the transformation of prion protein into an insoluble form. This study found that two phage chaperonins can promote the fibrillation of prion protein in an ATP-dependent manner, resulting in the formation of less toxic large clusters. These fibrils differ in morphology and properties from those formed spontaneously in acidic pH with denaturants.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Gaurab Chowdhury, Saroj Biswas, Yuthika Dholey, Puja Panja, Sumit Das, Subrata Adak
Summary: Magnesium is an important divalent cation for regulating enzyme activity. The binding of Mg2+ through the PAS domain inhibits phosphoglycerate kinase (PGK) activity in LmPAS-PGK at neutral pH, but PGK activity is derepressed at acidic pH. Mutation studies revealed that the Asp-4 residue is crucial for Mg2+ binding at neutral pH.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Shima Ghaedizadeh, Majid Zeinali, Bahareh Dabirmanesh, Behnam Rasekh, Khosrow Khajeh, Ali Mohammad Banaei-Moghaddam
Summary: Implementing hyperthermostable carbonic anhydrases into CO2 capture and storage technologies can increase the rate of CO2 absorption from industrial flue gases. This study successfully improved the thermostability of a known hyperthermostable carbonic anhydrase through rational engineering of a single-point mutation.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Khaled A. Elnahriry, Dorothy C. C. Wai, Lauren M. Ashwood, Muhammad Umair Naseem, Tibor G. Szanto, Shaodong Guo, Gyorgy Panyi, Peter J. Prentis, Raymond S. Norton
Summary: Sea anemone venom contains a peptide called Tst2, which shows sequence similarity to peptides that interact with various ion channels. Recombinant Tst2 was successfully produced and its structure and function were studied. The results showed that Tst2 is an inhibitor of the TRPV1 channel.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)