Article
Chemistry, Medicinal
Tanel Sormus, Darja Lavogina, Anu Teearu, Erki Enkvist, Asko Uri, Kaido Viht
Summary: This study reports the development of the first covalent bisubstrate PK inhibitor that reacts with a distant cysteine residue outside the ATP-binding site. The synthesized inhibitors demonstrated PKA-selectivity and targeting capability in cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Optics
Anastasiia Kharcheva, Zinaida A. Charyshnikova, Nataliya E. Borisova, Tsagana B. Sumyanova, Oleg K. Farat, Dmitriy A. Kharitonov, Svetlana Patsaeva
Summary: The luminescence characteristics of a new water-soluble europium complex were studied, revealing the existence of different species of the complex in different pH environments. This finding has significant implications for the development of a multimodal pH sensor for aqueous environments.
JOURNAL OF LUMINESCENCE
(2022)
Article
Chemistry, Applied
Jun Gong, Ruishan Huang, Chunfei Wang, Zujin Zhao, Ben Zhong Tang, Xuanjun Zhang
Summary: This research presents an easily post-modifiable CPL-active scaffold and proposes a feasible strategy to construct chiroptical probes for sensing hypochlorous acid. The compound exhibits good optical and chiroptical responses in vitro and shows potential for broad application in the design of more chiroptical probes.
Article
Biophysics
Tawfiq Alsulami, Nidhi Nath, Rod Flemming, Hui Wang, Wenhui Zhou, Jae-Hyuk Yu
Summary: Compared to traditional heterogeneous assays, a homogeneous immunoassay using engineered luminescent proteins was developed for the detection of FB1, showing high specificity, acceptable recovery, and no need for a secondary antibody or washing steps.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Hanyu Liang, Zhongzhu Hong, Shihua Li, Xiaorong Song, Da Zhang, Qiushui Chen, Juan Li, Huanghao Yang
Summary: Thrombin-activatable scintillating nanoprobes have been developed for background-free X-ray-excited luminescence (XEL) imaging of early thrombosis in vivo, by designing bright XEL-emitting lanthanide-doped scintillator nanocrystals with dye labeled-peptide modification. The strategy achieves high-efficiency XEL imaging of early thrombosis based on in situ elevated thrombin levels, demonstrating a novel paradigm for timely thrombosis imaging and providing a readily tailorable platform for sensitive in vivo deep-tissue imaging.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Engineering, Environmental
Ying-Qi Song, Ke-Jia Wu, Zhiming Zhang, Tzu-Ming Liu, Chung-Nga Ko, Wei-Guo Zhu, Dik-Lung Ma, Wanhe Wang, Chung-Hang Leung
Summary: In this study, a luminescent probe K293 was developed for screening BRD4 inhibitors. K293 selectively binds to BRD4 in living cancer cells and decreases its luminescence signal by competing with candidate compounds. This technique allows the identification of potential BRD4 inhibitors that cannot be detected by conventional fluorescence methods.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Inorganic & Nuclear
Changhua Su, Feng Guo
Summary: A novel luminescent MOF was successfully prepared through the coordination assembly of CdCl2 and BIM, forming a 3D skeleton based on a 1D Cd-based chain. The MOF exhibited good water stability and luminescence properties, showing luminescence turn-off towards Fe3+ and NFT, with high selectivity and reusability.
INORGANIC CHEMISTRY COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Chanyong Park, Abdurhaman Teyib Abafogi, Dinesh Veeran Ponnuvelu, Ilchan Song, Kisung Ko, Sungsu Park
Summary: This study focuses on improving the ATP luminescence assay for the detection of CTCs by using a 3D printed immunomagnetic concentrator (3DPIC) to separate and concentrate CTCs in the blood. The results show that 3DPIC can concentrate cancer cells in blood by 100 times within minutes, enabling the ATP luminescence assay to detect as low as 10 cells in blood, which is approximately 10 times more sensitive than using commercial kits for CTC concentration.
Article
Polymer Science
Ni Wu, Ruiqiang Jia, Haitao Hong, Hanyue Gao, Zhiyong Guo, Hongbing Zhan, Shaowu Du, Banglin Chen
Summary: After post-peroxide, the luminescent CTF displayed significant differences in sensing behaviors towards nitroaromatic compounds and metal ions compared to the pristine CTF. The underlying mechanism behind these differences in sensing ability was further investigated.
Article
Chemistry, Analytical
Chaolong Liu, Jianping Liu, Wenzhu Zhang, Yong-Lei Wang, Xiaona Gao, Bo Song, Jingli Yuan, Run Zhang
Summary: This study presents the development of a responsive Ru(II) complex probe Ru-PNBD for colorimetric and luminescent analysis of H2S in living cells and organisms. Ru-PNBD shows high sensitivity and selectivity for H2S detection, low cytotoxicity, and good permeability to cell membrane.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Alla Yegorova, Yuliia Skrypynets, Inna Leonenko, Axel Duerkop
Summary: This study presents a simple, sensitive, and validated method for determining the content of chlorogenic acid in green and roasted coffee, without the need for chromatographic separation. The method utilizes luminescence intensity quenching to measure the concentration of chlorogenic acid. The results showed that the method is effective and can be applied for quality control of chlorogenic acid in coffee.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ting Zhu, Ya Chen, Xiaoping Yang, Xilong Leng, Desmond Schipper
Summary: A novel 11-metal Zn(II)-Tb(III) nanocluster 1 was synthesized using a tetradentate Schiff base ligand, which exhibited enhanced Tb(III) luminescence upon addition of GMP and RNA. The nanocluster 1 showed highly sensitive detection of GMP and RNA with rapid and stable luminescence response.
Article
Chemistry, Physical
Zhi-Hui Nie, Lu Lu, Minyue Zheng, Zehao Liao, Gaomin Ye, Ayushi Singh, Abhinav Kumar
Summary: The newly designed Zn(II)-based coordination polymer shows sensitive and selective sensing against nitrofurazone (NZF) with a low limit of detection and a high Ksv value. The experimentally observed decline in luminescence intensity has been explained with theoretical calculations.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Agriculture, Multidisciplinary
Tianhao Jia, Chunmei Pu, Tianyi Qin, Bin Liu, Guangkai Yao, Zhiqing Xun, Bingfeng Wang, Yongqing Tian, Zhixiang Zhang, Hanhong Xu, Chen Zhao
Summary: In this study, a simple indicator displacement assay (IDA) based on an azo-modified calixarene host and a fluorophore guest was developed for the detection of paraquat in environmental water samples and plant surfaces. The probe exhibited the ability to recover the fluorescent signal of the fluorophore upon the addition of paraquat within 10 seconds. The detection range of the probe was calculated to be 0.35-8 μM in buffer solutions and showed excellent selectivity and anti-interference abilities.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Tanel Sormus, Darja Lavogina, Anu Teearu, Erki Enkvist, Asko Uri, Kaido Viht
Summary: This study reports the development of the first covalent bisubstrate PK inhibitor that reacts with a distant cysteine residue outside the ATP-binding site. The synthesized inhibitors demonstrated PKA-selectivity and targeting capability in cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Issiah B. Lozada, Jason D. Braun, J. A. Gareth Williams, David E. Herbert
Summary: This study presents a series of unique Zn(II) complexes with a pseudo-octahedral structure. By incorporating phenanthridine units into the ligands, these complexes exhibit extended luminescence lifetimes in the solid state. The research also indicates the role of aggregation in enhancing emission in solid state.
INORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Darja Lavogina, Naila Nasirova, Tanel Sormus, Taavo Tahtjarv, Erki Enkvist, Kaido Viht, Toiv Haljasorg, Koit Herodes, Jana Jaal, Asko Uri
Summary: In this research, we designed a series of ARCs and their fluorescent derivatives to investigate their interactions with members of the protein arginine methyltransferase (PRMT) family. Our results showed that ARCs and arginine-rich peptides could serve as high-affinity ligands for PRMT1, and the equilibrium dissociation constant values were dependent on the number of arginine residues within the compounds.
JOURNAL OF PEPTIDE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Tanel Sormus, Darja Lavogina, Erki Enkvist, Asko Uri, Kaido Viht
Summary: Bivalent ligands, such as bisubstrate inhibitors, can simultaneously target two binding sites of a biomolecule and their ability to bind to the biological target can be modulated by an external trigger. This study constructed deactivatable bisubstrate inhibitors of basophilic protein kinases by conjugating pharmacophores via linkers that can be cleaved in response to external stimuli. The inhibitors exhibited high affinity and inhibitory potency towards cAMP-dependent PK (PKAc alpha), which could be reduced by specific external stimuli. These deactivatable inhibitors are valuable tools for the inhibition or capture of pharmacologically important enzymes.
Article
Chemistry, Multidisciplinary
Etienne S. Gauthier, Laura Abella, Elsa Caytan, Thierry Roisnel, Nicolas Vanthuyne, Ludovic Favereau, Monika Srebro-Hooper, J. A. Gareth Williams, Jochen Autschbach, Jeanne Crassous
Summary: Describes the photophysical and chiroptical properties of a novel, chiral helicene-NHC-Re(I) complex with an N-(aza[6]helicenyl)-benzimidazolylidene ligand, showing its capability of emitting yellow circularly polarized luminescence. A comparative analysis with other helicene-Re(I) complexes reported to date highlights the impact of structural modifications on the emissive and absorptive properties.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Inorganic & Nuclear
Yana M. Dikova, Dmitry S. Yufit, J. A. Gareth Williams
Summary: Platinum(II) complexes of NNC-cyclometalating ligands based on 6-phenyl-2,2 '-bipyridine (HL1) have been extensively studied for their luminescence properties. Oxidation of PtL1Cl and its analogues with differently substituted aryl rings, PtL2-6Cl, by chlorine and/or iodobenzene dichloride generates Pt(IV) compounds of the form Pt(NNC-Ln)Cl3 (n = 1-6). These PtLnCl3 compounds react with 2-arylpyridines to form a new class of Pt(IV) complexes [Pt(NNC)(NC)Cl]+. The UV-vis absorption spectra and phosphorescence properties of these complexes are affected by the electron richness of the aryl ring.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Rebecca J. Salthouse, Amit Sil, Louise F. Gildea, Dmitry S. Yufit, J. A. Gareth Williams
Summary: 2,6-Dipyridylbenzene derivatives are known ligands for brightly luminescent Pt(II) complexes. In this study, nonsymmetrical systems of this type, where the two heterocycles are different, were investigated. The emission generally originates from the lower-energy π* orbitals of the heterocycle, but an isoquinoline complex showed dual emission at room temperature. Isoquinoline also favored the formation of bright excimers at higher concentrations. New platinum(II) complexes with seven different ligands were synthesized and their absorption and emission properties were compared with symmetrical complexes. In addition, the unusual emission behavior of (PtLCl)Cl_1, which involves both pyridine and isoquinoline rings, was attributed to thermally activated repopulation of the excited state.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Debsouri Kundu, Natalia del Rio, Marie Cordier, Nicolas Vanthuyne, Emma V. Puttock, Stefan C. J. Meskers, J. A. Gareth Williams, Monika Srebro-Hooper, Jeanne Crassous
Summary: The preparation, structural and spectroscopic characterization of the first enantiopure cycloplatinated complexes with a bidentate, helicenic N-heterocyclic carbene and a diketonate ancillary ligand were investigated through experimental and computational studies. These complexes exhibit long-lived circularly polarized phosphorescence, with dissymmetry factor g(lum) values >= 10(-3) in solution, doped films at room temperature, and around 10(-2) in a frozen glass at 77 K.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Etienne S. Gauthier, Dominika Kaczmarczyk, Samuel Del Fre, Ludovic Favereau, Elsa Caytan, Marie Cordier, Nicolas Vanthuyne, J. A. Gareth Williams, Monika Srebro-Hooper, Jeanne Crassous
Summary: This article presents the synthesis and properties of enantiopure copper(I) chloride complexes bearing a monodentate N-(carbo[6]helicenyl)-NHC ligand, focusing on their photophysical and chiroptical properties. Experimental results show that the complex emits circularly polarized blue fluorescence in solution with high stability.
DALTON TRANSACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Trang Van Tran, Hoa Nguyen, Luyen Vu, ChangWoo Lee
Summary: Glutaredoxin 3 (Grx3) is a redox protein that maintains structural integrity and glutathione (GSH) binding capabilities across different temperatures. This study investigates the roles of specific bonds in Grx3's structure and function, and how psychrophilic Grx3 variants adapt to cold environments. The highly conserved Arg51-Asp69 salt bridge and Gln56-His63 hydrogen bond are crucial for stabilizing the structure and catalytic activity of Grx3. Psychrophilic variants of Grx3 have adapted to cold environments by reducing GSH binding and increasing structural flexibility.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Amanda Lais de Souza Coto, Arthur Alexandre Pereira, Sabrina Dorta Oliveira, Milene Nobrega de Oliveira Moritz, Arthur Moraes Franco da Rocha, Paulo Roberto Dores-Silva, Noeli Soares Melo da Silva, Ana Rita de Araujo Nogueira, Lisandra Marques Gava, Thiago Vagas Seraphim, Julio Cesar Borges
Summary: J-domain proteins form a large molecular chaperone family involved in proteostasis processes, with hDjC20 playing a vital role in mitochondria and being heavily influenced by the presence of Zn+2.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Meiling Zhang, Jiaxiang Zhang, Yan Liang, Shicheng Tian, Shuyang Xie, Tong Zhou, Qin Wang
Summary: This study determined the crystal structures of RGLG2 VWA domain in Arabidopsis thaliana, revealing that Ca2+ ions act as regulators and affect the conformational change of RGLG2-VWA domain.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Alexandra Bork, Sander H. J. Smits, Lutz Schmitt
Summary: This study reveals the structure and calcium ion binding properties of CBL1 protein, and proposes a binding model of CBL1 for Ca2+. Additionally, it provides preliminary insights into the formation of the dimer interface of CBL1.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Evgeniia V. Leisi, Andrey V. Moiseenko, Sofia S. Kudryavtseva, Denis V. Pozdyshev, Vladimir I. Muronetz, Lidia P. Kurochkina
Summary: The pathogenesis of prion diseases involves the transformation of prion protein into an insoluble form. This study found that two phage chaperonins can promote the fibrillation of prion protein in an ATP-dependent manner, resulting in the formation of less toxic large clusters. These fibrils differ in morphology and properties from those formed spontaneously in acidic pH with denaturants.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Gaurab Chowdhury, Saroj Biswas, Yuthika Dholey, Puja Panja, Sumit Das, Subrata Adak
Summary: Magnesium is an important divalent cation for regulating enzyme activity. The binding of Mg2+ through the PAS domain inhibits phosphoglycerate kinase (PGK) activity in LmPAS-PGK at neutral pH, but PGK activity is derepressed at acidic pH. Mutation studies revealed that the Asp-4 residue is crucial for Mg2+ binding at neutral pH.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Shima Ghaedizadeh, Majid Zeinali, Bahareh Dabirmanesh, Behnam Rasekh, Khosrow Khajeh, Ali Mohammad Banaei-Moghaddam
Summary: Implementing hyperthermostable carbonic anhydrases into CO2 capture and storage technologies can increase the rate of CO2 absorption from industrial flue gases. This study successfully improved the thermostability of a known hyperthermostable carbonic anhydrase through rational engineering of a single-point mutation.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Khaled A. Elnahriry, Dorothy C. C. Wai, Lauren M. Ashwood, Muhammad Umair Naseem, Tibor G. Szanto, Shaodong Guo, Gyorgy Panyi, Peter J. Prentis, Raymond S. Norton
Summary: Sea anemone venom contains a peptide called Tst2, which shows sequence similarity to peptides that interact with various ion channels. Recombinant Tst2 was successfully produced and its structure and function were studied. The results showed that Tst2 is an inhibitor of the TRPV1 channel.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
