4.3 Article

Using polarization analysis to separate the coherent and incoherent scattering from protein samples

Journal

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2009.06.024

Keywords

Neutron scattering; Polarization analysis; Coherent/incoherent nuclear scattering; Protonated/deuterated protein; Structure; Dynamic

Funding

  1. Fundacao para Ciencia e Tecnologia [SFRH/BDP/17571/2004]
  2. Deutsche Forschungsgemeinschaft [SFB 533]

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Polarization analysis was used to separate experimentally the coherent and spin-incoherent nuclear static scattering functions, from a representative set of samples of interest for protein studies. This method had so far limited application in the study of amorphous materials, despite the relevance of the information that it provides. It allows, for instance, the experimental determination of the structure factor of materials containing a significant amount of hydrogen atoms, avoiding the contamination of measurements by a non-negligible incoherent background. Knowledge of the relative importance of the coherent and incoherent terms at different Q-values is also a pre-requisite for the interpretation of quasielastic neutron scattering experiments, performed at instruments in which the total dynamic scattering function is measured, such as conventional time-of-flight and backscattering spectrometers. Combining data from different instruments, it was possible to cover a wide Q-range, from the small-angle region (0.006

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