Article
Biochemistry & Molecular Biology
Yasunori Fukumoto, Masayoshi Ikeuchi, Yuji Nakayama, Yasumitsu Ogra
Summary: This study reveals that Rad17 protein has nucleolar localization signal and its accumulation in nucleolus is promoted by UV irradiation, suggesting a possible role of Rad17 in nucleolus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tetsuaki Miyake, John C. McDermott
Summary: This study reveals that the proto-oncogene c-Jun can be targeted to the nucleolus, and its basic domain contains a unique motif that determines nucleolar localization. While partners such as Fra2 do not co-localize with c-Jun in the nucleolus. A point mutation can result in loss of nucleolar targeting for c-Jun, while its nuclear localization remains intact.
Article
Biochemistry & Molecular Biology
Prajakta Tathe, K. V. S. Rammohan Chowdary, Krushna Chandra Murmu, Punit Prasad, Subbareddy Maddika
Summary: In this study, the researchers discovered that the tyrosine phosphatase SHP-1 can dephosphorylate histone H2B and plays a critical role in transcription. SHP-1 dephosphorylates H2B at tyrosine 121, promoting H2B ubiquitination and maintaining basal autophagic flux. This study reveals the importance of SHP-1-regulated modification of H2B in eukaryotic transcription.
Article
Microbiology
Jianwei Zhou, Yuexia Wang, Yonghui Qiu, Yongxia Wang, Xiaoyu Yang, Changzhe Liu, Yongyan Shi, Xufei Feng, Lei Hou, Jue Liu
Summary: The study found that PCV4 Cap mediated the translocation of DDX21 from the nucleolus to the cytoplasm and confirmed that the NoLS of PCV4 Cap directly bound to DDX21, demonstrating for the first time that DDX21 binds directly to the NoLS of the PCV4 Cap and contributes to its nucleolar localization.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Margarita A. Kurnaeva, Arthur O. Zalevsky, Eugene A. Arifulin, Olga M. Lisitsyna, Anna Tvorogova, Maria Y. Shubina, Gleb P. Bourenkov, Maria A. Tikhomirova, Daria M. Potashnikova, Anastasia Kachalova, Yana R. Musinova, Andrey Golovin, Yegor S. Vassetzky, Eugene Sheval
Summary: This study investigated the integration of nuclear localization signal (NLS) and nucleolar localization signal (NoLS) into the basic domain of HIV-1 Tat and found that these two supplementary functions are embedded within the amino acid sequence. Integration of NLSs and NoLSs into functional domains of viral proteins enriched with positively charged amino acids allows the concentration of different functions within small protein regions and may have influenced viral evolution by preventing an increase in protein size.
JOURNAL OF VIROLOGY
(2022)
Article
Plant Sciences
Zuntao Xu, Enze Li, Gan Xue, Cheng Zhang, Yachun Yang, Yong Ding
Summary: This study reveals that OsHUB2 represses the function of OsTrx1 and H3K4me3 levels at Ehd1, and suggests that OsHUB2-mediated H2B ubiquitination plays critical roles together with H3K4me3 in rice heading date regulation.
Article
Cell Biology
Sheila Q. Xie, Bryony J. Leeke, Chad Whilding, Ryan T. Wagner, Ferran Garcia-Llagostera, YiXuan Low, Paul Chammas, Nathan T-F Cheung, Dirk Dormann, Michael T. McManus, Michelle Percharde
Summary: Remarkably, our study reveals a close link between rRNA synthesis, nucleolar maturation, and gene repression during early development.
GENES & DEVELOPMENT
(2022)
Article
Cell Biology
Liying Wang, Zhiliang Xu, Libin Wang, Chao Liu, Huafang Wei, Ruidan Zhang, Yinghong Chen, Lina Wang, Wenwen Liu, Sai Xiao, Wei Li, Wei Li
Summary: The study reveals that RNF20 is crucial for early stage somatic cell reprogramming, with Rnf20 knockout leading to failure in reprogramming at the initial stage. The absence of RNF20 affects the transcription of MET-related genes and early pluripotency genes, ultimately hindering the establishment of a pluripotent gene network.
CELL PROLIFERATION
(2021)
Article
Biology
Li Zhang, Pan Luo, Jie Bai, Lei Wu, Dong-Wei Di, Hai-Qing Liu, Jing-Jing Li, Ya-Li Liu, Allah Jurio Khaskheli, Chang-Ming Zhao, Guang-Qin Guo
Summary: The study identified CKRW2 as a mutant form of HUB1 in Arabidopsis, which encodes an E3 ligase required for histone H2B monoubiquitination. The research suggests the involvement of H2Bub1 in regulating auxin biosynthesis.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Min Zhang, Fan Zheng, Yujie Xiong, Chen Shao, Chengliang Wang, Minhao Wu, Xiaojia Niu, Fenfen Dong, Xuan Zhang, Chuanhai Fu, Jianye Zang
Summary: Our study demonstrates that the interaction of spMis18 with histone H2A-H2B and DNA is crucial for the recruitment of spMis18 and Cnp1 to the centromere in fission yeast. Mutations in the acidic region and deletion of the extreme C-terminus of spMis18 significantly affect its localization and delay chromosome segregation during mitosis, indicating the importance of these interactions in faithful chromosome segregation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yi Ching Esther Wan, Kui Ming Chan
Summary: Oncohistones have become a new focus in cancer epigenetics research, with recent studies uncovering thousands of histone mutations in cancer. Unlike previously identified oncohistones, the newly discovered mutations are located within the histone fold domain and impact gene expression through different mechanisms. Recent characterization of selected H2B has highlighted previously unappreciated roles of oncohistones in nucleosome stability, chromatin accessibility, and chromatin remodeling.
Review
Cell Biology
M. Carmen Lafita-Navarro, Maralice Conacci-Sorrell
Summary: The nucleolus is a large nuclear organelle responsible for ribosome biogenesis. Errors in ribosomal biogenesis can cause nucleolar stress, leading to changes in nucleolar function and morphology. This review discusses the association of nucleolar stress with degenerative diseases and developmental defects, as well as the importance of de novo nucleotide biosynthesis for enhanced nucleolar activity in cancer cells and the potential targeting of nucleotide biosynthesis as a strategy to activate nucleolar stress and specifically target cancer cells.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Tasniem Fetian, Brendan M. McShane, Nicole L. Horan, Donya N. Shodja, Jason D. True, Amber L. Mosley, Karen M. Arndt
Summary: Histone modifications coupled to transcription elongation play important roles in regulating gene expression. The Paf1 transcription elongation complex (Paf1C) interacts with the ubiquitin conjugase Rad6 through its histone modification domain (HMD), leading to H2BK123 ubiquitylation. The specific interface between a transcription elongation factor and a ubiquitin conjugase guides substrate selection during active gene expression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Soumyadip Sahu, Jacob Gordon, Chunfang Gu, Mack Sobhany, Dorothea Fiedler, Robin E. Stanley, Stephen B. Shears
Summary: 5-InsP(7) regulates the binding of nucleolar proteins, and altering its levels can affect the volume of the nucleolar granular region in cells.
Article
Cell Biology
Shinya Takahata, Saori Chida, Aoi Ohnuma, Motoyoshi Ando, Takahiro Asanuma, Yota Murakami
Summary: In heterochromatin, Spt16 recruitment is mediated by Pob3 and HP1/Swi6. Pob3 recruits Spt16 through its Spt16 dimerization and PH domains, while HP1/Swi6 recruits Spt16 through physical interaction of the Swi6 CSD and Spt16 peptidase-like domains.
Article
Biochemistry & Molecular Biology
E. V. Stelmashook, N. S. Chetverikov, S. A. Golyshev, E. E. Genrikhs, N. K. Isaev
BIOCHEMISTRY-MOSCOW
(2020)
Article
Cell Biology
Kseniia Galkina, Anna N. Zyrina, Sergey A. Golyshev, Nataliia D. Kashko, Olga Markova, Svyatoslav S. Sokolov, Fedor F. Severin, Dmitry A. Knorre
EUROPEAN JOURNAL OF CELL BIOLOGY
(2020)
Article
Microbiology
Olga V. Popova, Tatyana A. Belevich, Sergey A. Golyshev, Igor I. Kireev, Vladimir V. Aleoshin
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2020)
Article
Plant Sciences
Ekaterina A. Lazareva, Alexander A. Lezzhov, Denis A. Chergintsev, Sergei A. Golyshev, Valerian V. Dolja, Sergey Y. Morozov, Manfred Heinlein, Andrey G. Solovyev
Summary: The movement protein BMB2 from Hibiscus green spot virus induces constriction of ER tubules, increasing the permeability of PD and facilitating intercellular virus spread. These reticulon-like proteins are also found in other RNA viruses, suggesting a common mechanism for viral spread.
Article
Biochemistry & Molecular Biology
Burkitkan Akbay, Diego Germini, Amangeldy K. Bissenbaev, Yana R. Musinova, Evgeny V. Sheval, Yegor Vassetzky, Svetlana Dokudovskaya
Summary: HIV-1 Tat activates the Akt/mTORC1 signaling pathway in B cells, leading to aberrant activation of AICDA and inhibition of AICDA transcriptional repressors c-Myb and E2F8. These disruptions may ultimately lead to increased genomic instability and proliferation, potentially causing B cell malignancies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Anna A. Skuredina, Anastasia S. Tychinina, Irina M. Le-Deygen, Sergey A. Golyshev, Natalya G. Belogurova, Elena V. Kudryashova
Summary: The highly cross-linked sulfobutyl ether derivative of beta-cyclodextrin in combination with the antibacterial drug moxifloxacin forms polymeric nanoparticles with efficient drug encapsulation and extended antibacterial activity, showcasing potential for new pharmaceutical formulations.
REACTIVE & FUNCTIONAL POLYMERS
(2021)
Review
Developmental Biology
M. A. Tikhomirova, E. V. Sheval
Summary: The morphogenetic processes during embryogenesis are determined by changes in the structure and functions of embryonic cells. The greatest plasticity of cellular organization is provided by membrane-less organelles or biomolecular condensates formed by liquid-liquid phase separation. The flexibility of biogenesis of biomolecular condensates and the high dynamics of their components allow for rapid changes in cellular organization, impacting cell fate and the course of embryogenesis.
RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Eugene V. Sheval
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Margarita O. Shedenkova, Elena Stelmashook, Sergey A. Golyshev, Elizaveta E. Genrikhs, Nickolay K. Isaev
Summary: This study investigates the effect of extracellular alkalosis on the neurotoxicity of zinc ions in rat cerebellar granule neurons. The results suggest that zinc ions can cause neuronal death, while extracellular alkalosis can reduce the toxic effect of zinc ions and promote neuronal survival.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Review
Cell Biology
Anna A. Valyaeva, Anastasia A. Zharikova, Eugene Sheval
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an unprecedented global public health emergency, with COVID-19 ranging from mild symptoms to multiorgan failure. Understanding the cellular tropism of SARS-CoV-2 is crucial to comprehend its transmission and the severe outcomes of COVID-19. Single-cell RNA-seq data has been used to predict cellular tropism, although not all susceptible cells may be infected in COVID-19 patients.
CELL BIOLOGY INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Anna A. Valyaeva, Maria A. Tikhomirova, Daria M. Potashnikova, Alexandra N. Bogomazova, Galina P. Snigiryova, Aleksey A. Penin, Maria D. Logacheva, Eugene A. Arifulin, Anna A. Shmakova, Diego Germini, Anastasia I. Kachalova, Aleena A. Saidova, Anastasia A. Zharikova, Yana R. Musinova, Andrey A. Mironov, Yegor S. Vassetzky, Eugene V. Sheval
Summary: An increased frequency of B-cell lymphomas is observed in HIV-1-infected patients. Although HIV-1 does not infect B cells, the Tat protein released from infected cells may potentially contribute to the development of B-cell lymphomas. Tat can modify gene expression in B cells, leading to subtle changes in cellular growth and chromosome stability.
Article
Biochemistry & Molecular Biology
Anna A. Skuredina, Tatiana Yu Kopnova, Anastasia S. Tychinina, Sergey A. Golyshev, Irina M. Le-Deygen, Natalya G. Belogurova, Elena Kudryashova
Summary: This study proposes a novel method for investigating the in vitro antibacterial drug activity, focusing on the interaction between fluoroquinolone moxifloxacin and sulfobutyl ether derivative of beta-cyclodextrin (SCD) and its polymer (SCDpol). The results show that the inclusion of the drug in SCD significantly enhances its antibacterial activity and prolongs the action. The presence of proteinaceous fimbriae on the bacteria surface further increases the adsorption of the drug carrier, making fimbriae-bearing strains more suitable for the proposed treatment.
Article
Microscopy
Daria M. Potashnikova, Tatiana N. Sotnikova, Olesya M. Shirokova, Oleg V. Zayratyants, Elena Yu. Vasilieva, Eugene V. Sheval
Summary: Recent studies have shown that cilia impairment is a common pathological feature of SARS-CoV-2-infected bronchial epithelial cells, but this has mainly been observed in cultured cells and animal models. In this study, the researchers used autopsy material and transmission electron microscopy to observe cilia impairment in infected bronchial epithelial cells directly. They found that only a small number of infected cells showed cilia impairment, while the majority of bronchial cells in the autopsy material had undisturbed ciliary layers. This suggests that most bronchial cells in infected patients do not die directly from the virus.
ULTRASTRUCTURAL PATHOLOGY
(2023)
Article
Virology
Margarita A. Kurnaeva, Arthur O. Zalevsky, Eugene A. Arifulin, Olga M. Lisitsyna, Anna Tvorogova, Maria Y. Shubina, Gleb P. Bourenkov, Maria A. Tikhomirova, Daria M. Potashnikova, Anastasia Kachalova, Yana R. Musinova, Andrey Golovin, Yegor S. Vassetzky, Eugene Sheval
Summary: This study investigated the integration of nuclear localization signal (NLS) and nucleolar localization signal (NoLS) into the basic domain of HIV-1 Tat and found that these two supplementary functions are embedded within the amino acid sequence. Integration of NLSs and NoLSs into functional domains of viral proteins enriched with positively charged amino acids allows the concentration of different functions within small protein regions and may have influenced viral evolution by preventing an increase in protein size.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)