Article
Biochemistry & Molecular Biology
Yuan Hu, Chenyang Qi, Jiaoyu Shi, Weiqiang Tan, Zhonghua Zhao, Yanyong Xu, Huijuan Wu, Zhigang Zhang
Summary: UCHL1 deficiency leads to severe kidney damage and proteinuria in podocytes, which is associated with ER stress, protein accumulation, and cell apoptosis. UCHL1 may be a potential target for preventing non-immune complex-mediated glomerulopathy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Neurosciences
Katarina Ziakova, Maria Kovalska, Ivana Pilchova, Katarina Dibdiakova, Maria Brodnanova, Michal Pokusa, Dagmar Kalenska, Peter Racay
Summary: A transient global brain ischemia leads to selective ischemic neurodegeneration, and the mechanism of such selective and delayed neurodegeneration is still uncertain. This study focused on the involvement of proteasomal and endoplasmic reticulum (ER) stress in ischemic neurodegeneration. Laser scanning confocal microscopy analysis was performed on brain slices from animals underwent global brain ischemia. The results suggest dysfunction of the ubiquitin proteasome system and consequent p53-induced expression of PUMA as the main mechanisms responsible for selective and delayed degeneration of hippocampal pyramidal neurons.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Cell Biology
Zhiping Mi, Steven H. Graham
Summary: This review focuses on the potential role of UCHL1 in the pathogenesis of neurodegenerative diseases and brain injury and recovery. It discusses the normal physiological functions of UCHL1, the effects of posttranslational modification sites and splice variants on UCHL1 function, mouse models with UCHL1 mutations and deletions, and the hypothesized role and pathogenic mechanisms of UCHL1 in neurodegenerative diseases and brain injury.
AGEING RESEARCH REVIEWS
(2023)
Article
Pharmacology & Pharmacy
Narathip Naradun, Krajang Talabnin, Kanyavee Issarangkul Na Ayuttha, Chutima Talabnin
Summary: Cholangiocarcinoma is a lethal malignancy with limited treatment options. Piperlongumine, a biologically active alkaloid, has shown potential as a therapeutic option for cholangiocarcinoma by inducing cancer cell death. Combination treatment with piperlongumine and a proteasome inhibitor further enhances the anti-cancer activity. This research provides valuable insights into the potential use of piperlongumine as an alternative therapy for cholangiocarcinoma.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Environmental Sciences
Yuting Wang, Lei Xu, Lu Peng, Changyu Fang, Qi Qin, Xuying Lv, Zixuan Liu, Bingwei Yang, Erqun Song, Yang Song
Summary: This study demonstrated for the first time that PBDEQ-induced oxidative protein damage in LO2 cells, providing a better understanding of the cytotoxicity of PBDEs and their metabolites.
Article
Cell Biology
Yanan Li, Shujing Li, Huijian Wu
Summary: Cells adapt to environmental stimuli by making changes to combat injury and increase stress tolerance. If the damage is too severe to repair, cells undergo apoptosis to protect the overall population. Protein aggregation can cause serious damage to the cells, but ubiquitin plays a crucial role in clearing these abnormal proteins. Dysregulation of ubiquitin-proteasome system and autophagy can lead to the development of diseases.
Article
Biochemistry & Molecular Biology
Mahmoud A. Desouky, Mina Y. George, Haidy E. Michel, Doaa A. Elsherbiny
Summary: This study found that roflumilast, a phosphodiesterase-4 inhibitor, has a neuroprotective effect in a rat model of Parkinson's disease. It reduces motor impairment, brain lesions, and loss of dopaminergic neurons, and decreases the accumulation of misfolded α-synuclein. Roflumilast enhances proteasome activity, inhibits endoplasmic reticulum stress and oxidative stress, and shows significant anti-apoptotic activity. This study suggests that roflumilast can be repurposed as a novel neuroprotective drug to restore protein homeostasis.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Review
Biochemistry & Molecular Biology
Yaping Liu, Runrong Ding, Ze Xu, Yuan Xue, Dongdong Zhang, Yujing Zhang, Wenjie Li, Xing Li
Summary: Alzheimer's disease is characterized by the formation of senile plaques and neurofibrillary tangles in the brain, but these pathologic biomarkers can only be detected after a sustained disease progression. The challenge in AD treatment is the lack of effective biomarkers for early diagnosis. The protein quality control system plays a crucial role in AD, as the accumulation of abnormal proteins is closely related to its dysfunction. Therefore, focusing on the protein quality control system can provide novel therapeutic strategies for preventing AD development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Jenny Lozada Ortiz, Marina Betancor, Sonia Perez Lazaro, Rosa Bolea, Juan J. J. Badiola, Alicia Otero
Summary: Chronic accumulation of misfolded proteins can cause endoplasmic reticulum stress, triggering the unfolded protein response. In this study, the expression of three endoplasmic reticulum stress markers, PERK, BiP, and PDI, were investigated in sheep affected by scrapie. The results showed significantly higher levels of these markers in scrapie-infected sheep compared to healthy controls. In addition, increased accumulation of ubiquitin aggregates was observed in the brains of scrapie-affected animals, suggesting impairment of the ubiquitin-proteasome system in natural scrapie. These findings suggest that these proteins may serve as biomarkers and therapeutic targets for prion diseases.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Respiratory System
Yue Zhu, Huimin Ju, Hongyan Lu, Wei Tang, Junying Lu, Qiuxia Wang
Summary: The study revealed a significant increase in apoptosis and total ubiquitinated proteins expression in BPD rats and AECII cultures, and the change of UPP was associated with ERS. Further experiments showed that MG132 treatment resulted in increased AECII apoptosis and ERS sensors expression, indicating UPP may play a role in ERS-induced AECII apoptosis under hyperoxia condition.
BMC PULMONARY MEDICINE
(2021)
Article
Neurosciences
Zhiping Mi, Hao Liu, Marie E. Rose, Xiecheng Ma, Daniel P. Reay, Jie Ma, Jeremy Henchir, C. Edward Dixon, Steven H. Graham
Summary: UCHL1 is a multifunctional protein expressed at high levels in neurons throughout the brain, with its hydrolase activity playing a crucial role in the recovery after neuronal injury and the pathogenesis of neurodegenerative diseases.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Biophysics
Jinhee Lee, Bryant J. Kane, Mukund Khanwalker, Koji Sode
Summary: The incidence of traumatic brain injury (TBI) is increasing yearly, highlighting the importance of timely diagnosis for improved patient outcomes. In this study, a novel system for rapid and one-step electrochemical detection of ubiquitin C-terminal hydrolase L1 (UCHL1) was developed. The system demonstrated fast detection, no need for washing steps, and wide concentration range in artificial serum samples, making it a promising candidate for on-site TBI detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Obstetrics & Gynecology
Meghan C. H. Ozcan, Lisa Cruz, Morgan F. F. Woodman, Fusun Gundogan, Kathryn J. J. Grive
Summary: The unique deubiquitinating enzyme UCHL1 regulates protein turnover only in oocytes, spermatogonia, and neurons. This study investigates the variation in UCHL1 expression across fetal maturation of oocytes to assess the lifelong ovarian reserve. The study found that UCHL1 expression in oocytes increases during ovarian development and reaches a plateau at 27 weeks gestational age, maintaining elevated levels until 36 weeks. This increase in expression is correlated with the increase in oocyte area, particularly during the transition of oocytes into primordial follicles.
REPRODUCTIVE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Radek Jorda, Veronika Molitorova, Eliska Pilarova, Veronika Vojackova, Eva Reznickova, Klara Svobodova, Karel Pauk, Ales Imramovsky, Vladimir Krystof
Summary: The comparative study examined new proteasome inhibitors based on salicylic acid-modified pseudo-tripeptides with aldehyde or vinylsulfone terminations. The study found that aldehydes were more effective than vinylsulfones in inhibiting proteasomes in cells, inducing the accumulation of polyubiquitinated proteins, GFP accumulation, and apoptotic cell death.
BIOORGANIC CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Li Ji, Huan Gu
Summary: The study showed that rhein has inhibitory effects on weight, inflammatory factors, and oxidative stress, and improves insulin resistance, blood lipid levels, and pathological injury. Moreover, rhein also affects ERs in peripheral blood and adipose tissue, significantly reducing cell apoptosis levels. Additionally, rhein treatment decreases adipogenic differentiation and increases p62 expression, while impacting PPAR gamma and INSR levels.
Article
Cell Biology
Jin A. Choi, Rupalatha Maddala, Shruthi Karnam, Nikolai P. Skiba, Robin Vann, Pratap Challa, Ponugoti Vasantha Rao
Summary: In this study, we found that vasorin expression levels were lower in the aqueous humour of glaucoma patients. The research also indicated the important role of vasorin in maintaining cell survival and inhibiting cellular responses induced by TGF-beta. These findings reveal the potential role of vasorin in the pathobiology of glaucoma.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Letter
Nutrition & Dietetics
Harshpal S. Sachdev, G. Bhanuprakash Reddy, Raghu Pullakhandam, Santu Ghosh, Hemalatha Rajkumar, Anura V. Kurpad
EUROPEAN JOURNAL OF CLINICAL NUTRITION
(2023)
Article
Multidisciplinary Sciences
Shalem Raj Padakandla, Taraprasad Das, Gumpili Sai Prashanthi, Kiran Kumar Angadi, S. Sreenivasa Reddy, G. Bhanuprakash Reddy, Sisinthy Shivaji
Summary: This study compared the gut mycobiomes of diabetic rats and diabetic rats with retinal changes with normal control rats, indicating dysbiosis at the genus level in both diabetic groups. The researchers speculated that increased pathogenic fungi may contribute to inflammatory status in both diabetic rats and rats showing retinal changes.
Letter
Nutrition & Dietetics
Raghu Pullakhandam, Santu Ghosh, Bharati Kulkarni, G. Bhanuprakash Reddy, Hemalatha Rajkumar, Umesh Kapil, Anura V. Kurpad, Harshpal S. Sachdev
EUROPEAN JOURNAL OF CLINICAL NUTRITION
(2022)
Article
Food Science & Technology
M. Nagaraju, Krishna K. Kalahasti, K. Prathap Reddy, Utkarsh R. Addi, M. Satyavani, G. Bhanuprakash Reddy, S. Sreenivasa Reddy
Summary: The functional food mix comprising turmeric, amla, and black pepper (TAB) showed a prophylactic effect against lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats. TAB partially ameliorated the increase in inflammatory cytokines and prevented lung histopathological changes. Furthermore, TAB suppressed ER stress and apoptotic markers in the lung.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Biochemistry & Molecular Biology
V. Sudhakar Reddy, G. Bhanuprakash Reddy
Summary: The small heat shock protein (sHsp) family includes proteins that are induced in response to external stimuli and those that are constitutively expressed. These proteins function as molecular chaperones, protecting cells and maintaining their structure. Short sequences derived from sHsps, known as mini-peptides/mini-chaperones, have shown therapeutic potential in various diseases due to their small size and easy uptake into cells. However, their limited stability and enzymatic degradation in circulation have hindered their therapeutic efficacy. Carrier molecules such as nanoparticles and cell penetration peptides have been used to enhance the effectiveness of sHsp mini-chaperones. This review highlights recent advances in the therapeutic potential of sHsp-derived mini-chaperones and strategies for improving their efficacy.
Review
Biochemistry & Molecular Biology
Digambar Kavitake, Palanisamy Bruntha Devi, Cedric Delattre, G. Bhanuprakash Reddy, Prathapkumar Halady Shetty
Summary: Exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) have unique characteristics that make them of great interest. Enterococcus spp. and other LAB genera have been extensively studied for their EPS production. Enterococcus EPS have various functional and technological properties with potential commercial applications. The review focuses on the characterization, bioactive potentials, and potential applications of Enterococcus EPS from different sources.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Public, Environmental & Occupational Health
Paromita Banerjee, Bhanuprakash G. Reddy, SubbaRao M. Gavaravarapu
Summary: This formative research study aims to explore the opportunities and barriers of developing and implementing a Workplace Health Programme (WHP) in India's IT and BPO sectors. Through this study, areas for nutrition, physical activity, and health education were identified, as well as the potential for utilizing existing facilities for physical activity and modifying the food environment. Junior employees were found to have an optimistic bias regarding their risk of developing NCDs.
WORK-A JOURNAL OF PREVENTION ASSESSMENT & REHABILITATION
(2022)
Article
Biochemistry & Molecular Biology
Pandarinath Savitikadi, Ramesh Gogulothu, Ayesha Ismail, Geereddy Bhanuprakash Reddy, Vadde Sudhakar Reddy
Summary: This study investigated the effects of a maternal low-protein diet and postnatal rehabilitation on muscle protein degradation in adult offspring. The results showed that chronic protein restriction led to muscle atrophy and increased protein degradation, while postnatal rehabilitation had little effect on these processes.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Nutrition & Dietetics
Pandarinath Savitikadi, Ravindranadh Palika, Raghu Pullakhandam, G. Bhanuprakash Reddy, S. Sreenivasa Reddy
Summary: Zinc deficiency has various adverse effects on the body, including growth retardation, loss of appetite, vascular diseases, cognitive and memory impairment, and neurodegenerative diseases. This study found that dietary zinc inadequacy affects neurotrophic factors and proteostasis in the brain. Zinc repletion could partially restore the changes in the ubiquitin-proteasome system, autophagy, gliosis, endoplasmic reticulum stress, and apoptosis markers induced by zinc deficiency.
NUTRITION RESEARCH
(2023)
Article
Food Science & Technology
Digambar Kavitake, Swati Tiwari, Irshad Ahmad Shah, Palanisamy Bruntha Devi, Cedric Delattre, G. Bhanuprakash Reddy, Prathapkumar Halady Shetty
Summary: In recent years, there has been increasing interest in exopolysaccharides (EPS) of microbial origin, particularly those produced by lactic acid bacteria (LAB). These EPS exhibit diverse structures and physico-chemical properties with many potential applications, including anti-microbial and immune-modulatory effects. This review focuses on the anti-bacterial, anti-fungal, anti-viral, and anti-biofilm properties of LAB EPS, discussing their modes of action against pathogens. Additionally, the potential roles of LAB EPS in food preservation, food safety, and human health are explored.
Article
Biochemistry & Molecular Biology
Prathap Reddy Kallamadi, Deepshika Esari, Utkarsh Reddy Addi, Rushendhiran Kesavan, Uday Kumar Putcha, Siddavaram Nagini, Geereddy Bhanuprakash Reddy
Summary: Patients with comorbidities of obesity and diabetes are at high risk of breast cancer development and worse outcomes. This study investigated the molecular mechanisms underlying the increased risks using a rat model. The results showed that obese rats had higher tumor incidence and shorter latency periods, as well as elevated levels of markers for cell proliferation and angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Paromita Banerjee, G. Bhanuprakash Reddy, Hrusikesh Panda, Kiran Kumar Angadi, Thirupathi Reddy, SubbaRao M. Gavaravarapu
Summary: This study aimed to assess the metabolic and lifestyle risk factors among IT employees in India. The results indicate a high risk of developing non-communicable diseases among employees in the IT industry, highlighting the need for lifestyle and workplace interventions.
Article
Nutrition & Dietetics
Tattari Shalini, Raghu Pullakhandam, Santu Ghosh, Bharati Kulkarni, Hemalatha Rajkumar, Harshpal S. Sachdev, Anura V. Kurpad, Geereddy Bhanuprakash Reddy
Summary: There is a high prevalence of vitamin B-12 and folate deficiency among Indian children and adolescents, especially in adolescents. Although there are regional variations, there are no rural-urban differences. These findings suggest the need for further investigation.
Article
Biochemistry & Molecular Biology
A. Prabakaran, Amit Alexander
Summary: The molecular interactions and mucoadhesive nature of chitosan-coated liposomes with mucin are crucial for the development of an effective drug delivery system. The positively charged SA-CH-LPs showed stronger interaction and better mucoadhesive properties compared to negatively charged SALPs, thereby improving drug retention in the nasal cavity and enhancing therapeutic efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
Summary: This study discovered an effective strategy for cargo sorting within exosomes by incorporating an appropriate cleavage site, providing further insight into the potential of exosomes as nano-shuttles bearing therapeutic biomolecules.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Pei -Gee Yap, Chee-Yuen Gan
Summary: This study collected the sequences of 128 tyrosinase inhibitory peptides and analyzed their hydrophobicity/hydrophilicity properties and amino acid profiles. Molecular docking analysis was used to investigate the binding interactions between peptides and tyrosinase. The study found that hydrophobic and/or polar neutral properties facilitate or stabilize peptide binding with tyrosinase, and short peptides with cysteine and tyrosine tend to bind to the active site of tyrosinase. These findings provide detailed explanations for the relationship between peptide/amino acid structures and tyrosinase inhibition, as well as potential anti-melanogenesis mechanisms for peptide-based treatments against skin hyperpigmentation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
Summary: The study found that CHMP4B and VPS4A play an important role in reversing GSDMD-mediated pyroptosis by facilitating cell membrane remodeling in endometrial carcinoma.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
Summary: Arc protein is found in glutamatergic neurons of vertebrates and can be transferred between neurons in extracellular vesicles (EVs). In glioma cells, Arc protein is also present in EVs and can facilitate the transfer of mRNA, potentially contributing to tumor progression and affecting synaptic plasticity in cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Summary: In this study, it was demonstrated for the first time that FAT1 inhibited AML proliferation by reducing autophagy level. FAT1 achieved this by decreasing the expression of ATG4B, which is related to autophagy. Mechanistically, FAT1 decreased the levels of phosphorylated and intranuclear smad2/3, thus decreasing the activity of the ATG4B gene promoter. It was also found that FAT1 competitively bound to TGF-beta R II, leading to reduced phosphorylation of TGF-beta R I and smad2/3. Knockdown of FAT1 promoted AML autophagy and proliferation in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Summary: This study investigated the effects of a high-fat high-fructose diet (HFFD) on the behavior, energy metabolism, and oxidative stress markers in the cerebral cortex of mice. The results showed that HFFD stimulated locomotion and defecation, while an AKG-supplemented diet had a tendency to promote anxiety-like behavior. Additionally, there were significant differences in glutathione-dependent detoxification and processes related to autophagy between the two diets.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Summary: The study found that mitochondrial calpain-5 plays an important role in the occurrence and development of ischemia-reperfusion injury and is expressed in the human and mouse brains. Targeting the expression or activity of mitochondrial calpain-5 may have significant implications for suppressing inflammation during I/R injuries such as cerebrovascular diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aline Dias da Purificaca, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Veronica de Mello Gabriel, Joao Wosniak Junior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
Summary: The ER transmembrane chaperones DNAJB12 and DNAJB14 play important roles in protein folding and ER stress response. They are regulated by thiol redox processes and are involved in ER protein reflux.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Summary: Zinc deficiency can worsen obesity-related lung damage, and Nrf2 activation is one of the important mechanisms of this protective effect. Regulating zinc homeostasis can contribute to the prevention and treatment of obesity-related lung injury.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)