Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1808, Issue 4, Pages 1196-1201Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2011.01.007
Keywords
Channel; Negative stain; Pore; Ceramide; Sphingolipid; Apoptosis
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Funding
- National Science Foundation [MCB-0641208]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1023008] Funding Source: National Science Foundation
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Functional studies have shown that the sphingolipid ceramide, self-assembles in phospholipid membranes to form large channels capable of allowing proteins to cross the membrane. Here these channels are visualized by negative stain transmission electron microscopy. The images contain features consistent with stain-filled pores having a roughly circular profile. There is no indication of tilt, and the results are consistent with the formation of right cylinders. The sizes of the pores range from 5 to 40 nm in diameter with an asymmetric distribution indicating no apparent upper size limit. The size distribution matches well with the distribution of sizes calculated from electrophysiological measurements. (C) 2011 Elsevier B.V. All rights reserved.
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