Review
Cell Biology
MelissaR Pitman, Martin K. Oehler, Stuart M. Pitson
Summary: Ovarian cancer is a deadly gynaecological malignancy commonly diagnosed at an advanced stage, making treatment challenging. Novel strategies for early detection and therapeutics are urgently needed to improve patient survival, with the sphingolipid pathway showing promise as a potential therapeutic target against chemotherapy-resistant disease.
CELLULAR SIGNALLING
(2021)
Article
Multidisciplinary Sciences
Quist Kanyomse, Xin Le, Jun Tang, Fengsheng Dai, Youchaou Mobet, Chang Chen, Zhaobo Cheng, Chaoqun Deng, Yijiao Ning, Renjie Yu, Xiaohua Zeng, Tingxiu Xiang
Summary: KLF15 acts as a tumor suppressor in TNBC by downregulating CCL2 and CCL7, which inhibits cell growth and metastasis. KLF15 may serve as a prognostic biomarker in TNBC.
SCIENTIFIC REPORTS
(2022)
Article
Endocrinology & Metabolism
Sardar Sindhu, Yat Hei Leung, Hossein Arefanian, S. R. Murthy Madiraju, Fahd Al-Mulla, Rasheed Ahmad, Marc Prentki
Summary: Sphingolipids, especially ceramides, are crucial in pathophysiological processes related to metabolic syndrome, influencing insulin resistance, type 2 diabetes, and other diseases. Among sphingomyelinases, neutral sphingomyelinase 2 (nSMase2) appears to be particularly important in inflammation-related pathologies, with a feed-forward interaction mechanism involving proinflammatory cytokines. Inhibiting nSMase2 may have therapeutic benefits against inflammation-driven cardiometabolic diseases.
Review
Nutrition & Dietetics
Eunkyeong Kim, Sookyoung Jeon
Summary: The prevalence of obesity and related metabolic diseases has increased dramatically worldwide. Dysregulated ceramide metabolism appears to be a key feature in the pathogenesis of obesity-related metabolic diseases. This review discusses how numerous phytochemicals are able to alleviate ceramide-induced metabolic defects and reduce the risk of obesity-related metabolic diseases via diverse mechanisms.
Article
Cell Biology
Jiyoon L. Kim, Beatriz Mestre, Sun-Hye Shin, Anthony H. Futerman
Summary: Sphingolipids are a crucial family of lipids in cell function and survival, and recent research in this field has been led by a closely-knit group of researchers, including the late Dr. Lina Obeid. Dr. Obeid's valuable contributions to sphingolipid research centered on the "many-worlds" view of ceramides and the role of ceramide synthases (CerS) in the sphingolipid metabolic pathway.
CELLULAR SIGNALLING
(2021)
Article
Food Science & Technology
Philippe Guerre, Caroline Gilleron, Maria Matard-Mann, Pi Nyvall Collen
Summary: Fumonisins have significant effects on sphingolipids in the heart and gizzard of chickens, but no significant effects were observed in breast muscle. There are significant differences in sphingolipid composition between the heart and gizzard.
Article
Biochemistry & Molecular Biology
Aiping Bai, Jacek Bielawski, Alicja Bielawska, Yusuf A. Hannun
Summary: This study describes the synthesis and evaluation of erythro-enantiomers in comparison with threo-enantiomers of B13, revealing that erythro-enantiomers are more effective in inhibiting the growth of MCF7 cells. The results also indicate that erythro-enantiomers are more cell permeable and capable of hydrolysis and phosphorylation in cells. Moreover, the study shows the de-acylation and re-acylation of the amino group of the aromatic alcohol from the full set of B13 isomers within the cells.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Food Science & Technology
Didier Tardieu, Maria Matard-Mann, Pi Nyvall Collen, Philippe Guerre
Summary: The study analyzed the effects of FB on hepatic and plasma sphingolipidome in chickens, showing that alterations in sphingolipids can lead to toxic manifestations and can potentially serve as a biomarker of exposure to FB.
Article
Biochemistry & Molecular Biology
Xuewei Zhang, Wataru Sakamoto, Daniel Canals, Masumi Ishibashi, Masaya Matsuda, Kentaro Nishida, Masafumi Toyoshima, Shogo Shigeta, Makoto Taniguchi, Can E. Senkal, Toshiro Okazaki, Nobuo Yaegashi, Yusuf A. Hannun, Takeshi Nabe, Kazuyuki Kitatani
Summary: Regulation of sphingolipid metabolism has a significant impact on cellular homeostasis and cancer progression. Dysregulation of ceramide-centered metabolism is associated with metastatic potential in ovarian cancer cells. Specifically, the CerS2-C-24:1-ceramide axis, possibly counteracted by neutral ceramidase, plays a key role in limiting cell motility and metastatic potential.
Article
Biochemistry & Molecular Biology
Linda Sasset, Kamrul H. Chowdhury, Onorina L. Manzo, Luisa Rubinelli, Csaba Konrad, J. Alan Maschek, Giovanni Manfredi, William L. Holland, Annarita Di Lorenzo
Summary: Disruption of sphingolipid homeostasis and signaling can cause various diseases. However, the mechanisms of cellular sensing and regulation of sphingolipid homeostasis are still not well understood. This study identifies S1P as a key sphingolipid sensed by cells to maintain homeostasis, and S1P-S1PR signaling stabilizes ORMDLs to restrain SPT activity. Disruption of the S1PR/ORMDL axis leads to ceramide accrual, mitochondrial dysfunction, and endothelial dysfunction, which are early events in cardio- and cerebrovascular diseases.
Article
Immunology
Michihito Kyo, Zhaozhong Zhu, Ryohei Shibata, Michimasa Fujiogi, Jonathan M. Mansbach, Carlos A. Camargo, Kohei Hasegawa
Summary: This study identified specific nasopharyngeal lipids that can differentiate between respiratory syncytial virus (RSV) and rhinovirus (RV) bronchiolitis in infants. These lipids, including phosphatidylcholine (18:2/18:2), dihydroceramide (16:0), and eicosapentaenoic acid, are also associated with the severity of bronchiolitis.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Raquel Roman Faedo, Gabriel da Silva, Rodrigo Moreira da Silva, Tatiane Resende Ushida, Ricardo Roberto da Silva, Riccardo Lacchini, Leandro Luongo Matos, Luiz Paulo Kowalski, Noberto Peporine Lopes, Andreia Machado Leopoldino
Summary: Enzymes related to sphingolipids metabolism have been found to be altered in oral squamous cell carcinoma (OSCC). This study identified specific sphingolipid molecules with potential diagnostic and prognostic value in OSCC patients, both in plasma and tumor tissues. These findings suggest that sphingolipid signatures could serve as biomarkers for diagnosis and prognosis in OSCC.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Immunology
Samar M. M. Hammad, Olivia C. Harden, Dulaney A. Wilson, Waleed O. Twal, Paul J. Nietert, Jim C. Oates
Summary: SLE is a chronic autoimmune disease with African American SLE patients showing higher levels of certain sphingolipids, which are correlated with the severity of atherosclerosis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Neurosciences
Jonathan D. Teo, Oana C. Marian, Alanna G. Spiteri, Madeline Nicholson, Huitong Song, Jasmine X. Y. Khor, Holly P. McEwen, Anjie Ge, Monokesh K. Sen, Laura Piccio, Jessica L. Fletcher, Nicholas J. C. King, Simon S. Murray, Jens C. Bruening, Anthony S. Don
Summary: The sphingolipids GalCer, ST, and SM are crucial for myelin stability and function. By deleting the CerS2 gene and inhibiting the synthesis of C22-C24 sphingolipids, researchers found that the absence of these sphingolipids affects myelin biosynthesis and stability, leading to neurodegeneration. This study highlights the importance of lipid chain length in maintaining the structural integrity of myelin and provides insights into the molecular mechanism of microglial activation.
Article
Biochemistry & Molecular Biology
Maftuna Shamshiddinova, Shokhid Gulyamov, Hee-Jung Kim, Seo-Hyeon Jung, Dong-Jae Baek, Yong-Moon Lee
Summary: Sphingosine-1-phosphate (S1P) is a critical signaling molecule involved in various diseases, and the regulation of SPHK/S1P axis plays a key role in anticancer strategies. DPF-543 induces cytotoxic ceramides by enhancing SPT activity and inhibiting SPHKs, leading to Cers accumulation via de novo pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Johnny Stiban, Meenu Perera
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2015)
Article
Biology
Majdouline Abou-Ghali, Johnny Stiban
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2015)
Review
Biochemistry & Molecular Biology
Johnny Stiban, Minyoung So, Laurie S. Kaguni
BIOCHEMISTRY-MOSCOW
(2016)
Article
Endocrinology & Metabolism
Dureen Samandar Eweis, Fida Abed, Johnny Stiban
OBESITY RESEARCH & CLINICAL PRACTICE
(2017)
Article
Biochemistry & Molecular Biology
Yael Pewzner-Jung, Hyejung Park, Elad L. Laviad, Liana C. Silva, Sujoy Lahiri, Johnny Stiban, Racheli Erez-Roman, Britta Bruegger, Timo Sachsenheimer, Felix Wieland, Manuel Prieto, Alfred H. Merrill, Anthony H. Futerman
JOURNAL OF BIOLOGICAL CHEMISTRY
(2010)
Article
Biochemistry & Molecular Biology
Johnny Stiban, Gregory A. Farnum, Stacy L. Hovde, Laurie S. Kaguni
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Liana C. Silva, Oshrit Ben David, Yael Pewzner-Jung, Elad L. Laviad, Johnny Stiban, Sibali Bandyopadhyay, Alfred H. Merrill, Manuel Prieto, Anthony H. Futerman
JOURNAL OF LIPID RESEARCH
(2012)
Article
Biochemistry & Molecular Biology
Sandra N. Pinto, Elad L. Laviad, Johnny Stiban, Samuel L. Kelly, Alfred H. Merrill, Manuel Prieto, Anthony H. Futerman, Liana C. Silva
JOURNAL OF LIPID RESEARCH
(2014)
Review
Biochemistry & Molecular Biology
Muna Abed Rabbo, Yara Khodour, Laurie S. Kaguni, Johnny Stiban
Summary: Sphingolipids and their metabolism have become a hotly debated topic in various biochemical fields, impacting health and causing genetic sphingolipid storage diseases. These diseases mainly affect the neuronal and immune systems, with potential therapeutic strategies being discussed in this review.
LIPIDS IN HEALTH AND DISEASE
(2021)
Article
Genetics & Heredity
Minyoung So, Johnny Stiban, Grzegorz L. Ciesielski, Stacy L. Hovde, Laurie S. Kaguni
Summary: Recent evidence indicates that iron-sulfur clusters in DNA replicative proteins sense DNA-mediated charge transfer to regulate nuclear DNA replication. The presence of an ISC and a metal cofactor in the Dm mtDNA helicase was confirmed, with the NTD also playing a role in membrane binding. The NTD binds to liposomes mimicking phospholipid membranes through electrostatic interactions, with a higher specificity as cardiolipin content increases.
FRONTIERS IN GENETICS
(2021)
Article
Critical Care Medicine
Anna Herminghaus, Henrike Papenbrock, Rebecca Eberhardt, Christian Vollmer, Richard Truse, Jan Schulz, Inge Bauer, Adelheid Weidinger, Andrey V. Kozlov, Johnny Stiban, Olaf Picker
INTENSIVE CARE MEDICINE EXPERIMENTAL
(2019)
Meeting Abstract
Medicine, General & Internal
J. Stiban, D. Eweis
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2017)
Meeting Abstract
Medicine, General & Internal
J. Stiban, M. Perera
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2016)
Meeting Abstract
Biochemistry & Molecular Biology
Laurie Kaguni, Johnny Stiban, Gregory Farnum, Stacy Hovde, Minyoung So
Article
Biochemistry & Molecular Biology
Philipp Grad, Katarina Edwards, Lars Gedda, Victor Agmo Hernandez
Summary: This study investigates the effects of polyethylene glycol-(PEG) modified lipids and gangliosides on the Ca2+ induced interaction between liposomes composed of palmitoyl-oleoyl phosphatidylethanolamine (POPE) and palmitoyl-oleoyl phosphatidylserine (POPS) at physiological ionic strength. The results show that naked liposomes tend to adhere, rupture, and collapse on each other's surfaces upon addition of Ca2+, eventually resulting in the formation of large multilamellar aggregates and bilayer sheets. However, the presence of gangliosides or PEGylated lipids leads to the formation of small, long-lived bilayer fragments/disks. PEGylated lipids seem to be more effective than gangliosides at stabilizing these structures. The study suggests that direct liposome-liposome fusion is not the dominating process triggered by Ca2+ in the systems studied.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Saurabh Saraswat, Archana Chugh
Summary: Cell penetrating peptides (CPP) are important tools for intracellular delivery of molecules. In this study, a novel marine-derived CPP from Engraulis japonicus, called Engraulisin, was reported. Engraulisin demonstrated successful cellular uptake and selective antimicrobial activity against Methicillin-resistant Staphylococcus aureus (MRSA), making it a potential candidate for drug delivery.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Emanuela Efodili, Ashlynn Knight, Maryem Mirza, Cedric Briones, Il-Hyung Lee
Summary: This study demonstrates the spontaneous transfer of small membrane-bound peptides between a supported lipid bilayer and giant unilamellar vesicles in vitro. The transfer occurs through the formation of hemi-fusion stalks and is limited to small peptides due to the highly curved structure of the stalk. This system provides a synthetic platform for studying peptide trafficking between synthetic membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Yueqi Niu, Si Jia Chen, Jeffery B. Klauda
Summary: Macrophage membranes in the activated state are more tightly packed, exhibit increased chain order across lipid species, and form specific lipid clusters. These findings provide physiologically accurate models for future computational studies of macrophage membranes and their proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Niki Baccile, Vincent Chaleix, Ingo Hoffmann
Summary: This study measured the bending rigidity of self-assembled structures formed by a new biobased glucolipid bioamphiphile using neutron spin-echo (NSE) technology. The results showed that the bending rigidity of these structures is lower or higher compared to phospholipid membranes, providing a new theoretical basis for the application of these new molecular systems.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Alain Bolano Alvares, Pablo E. A. Rodriguez, Gerardo D. Fidelio
Summary: The behavior of amphiphilic molecules at the air/water interface was studied to evaluate the arrangement formed in a confined area. The surface properties of zwitterionic DPPC lipid and A beta(1-40) amyloid peptide in mixed films were investigated at different temperatures. DPPC undergoes a phase transition depending on temperature and lateral pressure, which allows for the study of its influence on amyloid structure formation.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Lukasz Plachta, Marzena Mach, Magdalena Kowalska, Pawel Wydro
Summary: Resveratrol, a popular phytoalexin found in grapes and red wine, has beneficial effects on the cardiovascular system, exhibits antiviral, antibacterial, and antifungal properties, and may have therapeutic effects against cancer. This study investigates the interaction of resveratrol with model cell membranes, demonstrating changes in their physicochemical parameters and highlighting the role of cholesterol content in resveratrol incorporation. The findings suggest that the molecular mechanism of action of resveratrol may involve interactions with lipid rafts.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Edurne Rujas, Beatriz Apellaniz, Johana Torralba, David Andreu, Jose M. M. Caaveiro, Shixia Wang, Shan Lu, Jose L. Nieva
Summary: This study investigates the role of fusion peptide and Trp-rich membrane proximal external region in HIV-1 fusion and demonstrates that liposome-based formulations containing FP-MPER hybrid peptides can induce the production of specific neutralizing antibodies in rabbits. The results support the use of liposomes as vaccine carriers and the inclusion of lipid membranes in immunogens to elicit specific humoral responses.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Dailin Li, Dingyuan Shi, Lei Wang
Summary: G protein-gated inwardly rectifying potassium (GIRK) channels play a significant role in regulating cell excitability. This study investigated the ion permeation mechanism in GIRK2 mutants using molecular dynamic simulations and QM/MM methods. The results revealed the importance of a multi-ion distribution for ion conduction.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Max J. den Uijl, Arnold J. M. Driessen
Summary: Membrane protein insertion into and translocation across the bacterial cytoplasmic membrane are essential processes facilitated by the Sec translocon. The phospholipid dependence of membrane protein insertion has remained mostly unknown. This study reveals that the insertion of the mannitol permease MtlA into the membrane depends on the presence of phosphatidylglycerol and is stimulated by phosphatidylethanolamine.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick Allen, Adam C. Smith, Vernon Benedicto, Abbas Abdulhasan, Vasanthy Narayanaswami, Enrico Tapavicza
Summary: We explore the stability, size, and structure of nanodiscs formed by the N-terminal domain of apolipoprotein E3 and a variable number of 1,2-dimyristoyl-sn-glycero-3-phosphocholine molecules. Our simulations suggest that nanodiscs containing 240 to 420 DMPC molecules are stable. The antiparallel configuration shows more protein-protein interactions and ionic contacts, as well as greater stability and rigidity compared to the parallel configuration.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick M. Keating, Nicholas P. Schifano, Xinrui Wei, Matthew Y. Kong, Jinwoo Lee
Summary: Lassa virus (LASV) infection is dependent on the fusion of its viral membrane with the host cell membrane, and our study reveals that this fusion process involves pH-dependent conformational changes in the transmembrane domain of LASV. These findings provide valuable insights into the fusion mechanism of LASV and can be utilized in the design of therapeutics to combat Lassa virus infections and prevent its potential spread.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Ashley L. Bennett, Kristen N. Cranford, Austin L. Bates, Christopher R. Sabatini, Hee-Seung Lee
Summary: This study employed molecular dynamics simulations to elucidate the binding, folding, and insertion details of TP10W in the cell membrane. The simulations revealed that TP10W can translocate across the lipid membrane as a monomer, and the charged amino acid side chains can move between lipid leaflets. Comparison with point mutated variants demonstrated significant conformational changes due to charge distribution.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Jessica H. van Wonderen, Jason C. Crack, Marcus J. Edwards, Thomas A. Clarke, Gerhard Saalbach, Carlo Martins, Julea N. Butt
Summary: Electrogenic bacteria transfer excess respiratory electrons to metal oxide particles and electrodes through the MtrCAB complex. The crystal structure of MtrCAB from S. baltica OS185 was resolved, and liquid chromatography mass spectrometry was used to determine the mass values of the three proteins in purified MtrCAB complexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Review
Biochemistry & Molecular Biology
Yi-Ming Chen, Ching-Tai Lu, Chia-Wen Wang, Wolfgang B. Fischer
Summary: A series of dye ligands were ranked based on docking experiments, selecting the most suitable ligands for binding with different proteins. Additionally, a comparison study was conducted on a series of repurposing drugs and known antivirals.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
