Review
Biochemistry & Molecular Biology
Alessandra Ferramosca, Vincenzo Zara
Summary: Yeast mitochondrial carriers play a crucial role in cellular metabolism by connecting mitochondrial and cytosolic reactions, contributing to various important metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Bin Zhang, Mesfin Geberekidan, Zhao Yan, Xia Yi, Jie Bao
Summary: A highly thermotolerant strain of Saccharomyces cerevisiae, S. cerevisiae Z100, was obtained through laboratory adaptive evolution. The cell survival ratio of S. cerevisiae Z100 at 50 degrees C increased by 1.2 times compared to the parental strain, resulting in higher ethanol production during cellulosic ethanol fermentation. Transcriptome analysis suggested that enhanced trehalose and glycogen synthesis, as well as carbon metabolism, contributed to the improved thermotolerance and ethanol fermentability of S. cerevisiae Z100.
FERMENTATION-BASEL
(2023)
Article
Biology
Tianyao Xiao, Viplendra P. S. Shakya, Adam L. Hughes
Summary: Deficiencies in mitochondrial import result in the toxic accumulation of non-imported precursor proteins, with some being redirected to the endoplasmic reticulum (ER). The ER delivery of endogenous mitochondrial trans-membrane proteins, particularly from the SLC25A family, is dependent on the GET complex. Loss of the GET pathway poses detrimental effects on yeast cells experiencing mitochondrial import failure.
LIFE SCIENCE ALLIANCE
(2021)
Article
Agriculture, Multidisciplinary
Jie Qiu, Kangxin Hou, Qiang Li, Jialin Chen, Xiwen Li, Hongping Hou, Liqiang Wang, Jia Liu, Qiang Xue, Caixia Wang
Summary: This study successfully achieved significant improvement in CBD production through de novo biosynthesis in Saccharomyces cerevisiae, with BPT1 identified as the most effective transporter for transferring CBGA to the vacuole. The CBD yield was increased by 100-fold, laying the foundation for CBD supply.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Cell Biology
Jonathan Tai, Rachel M. Guerra, Sean W. Rogers, Zixiang Fang, Laura K. Muehlbauer, Evgenia Shishkova, Katherine A. Overmyer, Joshua J. Coon, David J. Pagliarini
Summary: The study reveals that Hem25p, a mitochondrial glycine transporter required for haem biosynthesis, also acts as an isopentenyl pyrophosphate (IPP) transporter in Saccharomyces cerevisiae, playing a crucial role in coenzyme Q synthesis.
NATURE CELL BIOLOGY
(2023)
Article
Food Science & Technology
Juan Antonio Porras-Aguera, Jaime Moreno-Garcia, Teresa Garcia-Martinez, Juan Moreno, Juan Carlos Mauricio
Summary: The prise de mousse stage during the elaboration of sparkling wine through the traditional method involves a second fermentation in a sealed bottle, which significantly contributes to the unique organoleptic properties of these wines. This study characterizes the mitochondrial response of Saccharomyces cerevisiae strains used in sparkling wine production and found that CO2 overpressure negatively affects mitochondrion-related proteins. However, proteins involved in wine aromas and respiratory chain are found to be over-represented in sealed bottles, with differences in concentrations observed depending on time and CO2 overpressure parameters. Ethanol content during fermentation may be a key factor in these proteomic changes, highlighting the importance of further research in this area.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lin Zhang, Wenyan Song, Tingting Li, Yajuan Mu, Pan Zhang, Jingyan Hu, Houwen Lin, Jian Zhang, Hai Gao, Liang Zhang
Summary: APSK2 enhances excess PAPS production and promotes cancer development through its dynamic substrate recognition mechanism.
Article
Plant Sciences
Aizat Bekturova, Dinara Oshanova, Poonam Tiwari, Zhadyrassyn Nurbekova, Assylay Kurmanbayeva, Aigerim Soltabayeva, Dmitry Yarmolinsky, Sudhakar Srivastava, Veronika Tureckova, Miroslav Strnad, Moshe Sagi
Summary: The study revealed the important roles of adenosine-5'-phosphosulphate reductase (APR) and sulfite oxidase (SO) in sulfite homeostasis, affecting water consumption and stomatal aperture in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Talis Uelisson da Silva, Karina de Carvalho Pougy, Magaly Girao Albuquerque, Camilo Henrique da Silva Lima, Sergio de Paula Machado
Summary: This study used molecular dynamics (MD) simulations to evaluate chelating agents as potential inhibitors of dissimilatory adenosine-5'-phosphosulfate reductase (APSrAB). Molecular docking and MD simulations were used to study the binding modes and energies of these agents. The results showed that the selected ligands had favorable binding at both the substrate and active sites of the enzyme, indicating their potential as inhibitors for further experimental studies.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Sha Xu, Guangjian Li, Jingwen Zhou, Guicai Chen, Jianzhong Shao
Summary: This study successfully engineered Saccharomyces cerevisiae for efficient anthocyanin production through metabolic engineering, providing new methods and approaches for anthocyanin production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yinkun Jiang, Lu Xia, Song Gao, Ning Li, Shiqin Yu, Jingwen Zhou
Summary: This study developed a sustainable strategy to enhance the biosynthesis of (-)-alpha-bisabolol by metabolic engineering. By introducing the heterologous gene MrBBS and weakening the competitive pathway gene ERG9, a strain capable of producing 221.96 mg/L (-)-alpha-bisabolol was constructed. Further optimization of the pathway and overexpression of PDR15 led to a production of 7.02 g/L in a 5 L bioreactor, the highest reported titer in yeast to date. This work provides a reference for efficient production of (-)-alpha-bisabolol in yeast.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Microbiology
Yoshihiro Ojima, Naoki Yokota, Yuki Tanibata, Shinsuke Nerome, Masayuki Azuma
Summary: This study investigated the mechanism of toxicity of Toyocamycin (TM) and its analogs to Candida albicans. It was found that the absence of purine nucleoside transport activity from Saccharomyces cerevisiae (S. cerevisiae) made it less susceptible to TM and its analogs. However, when the concentrative nucleoside transporter (CNT) of Candida albicans (C. albicans) was expressed in S. cerevisiae, the sensitivity to TM and its analogs was restored. In addition, introducing human CNT3 into S. cerevisiae also made it sensitive to TM and its analogs. These findings enhance our understanding of the mechanisms of action of adenosine analogs toward Candida pathogens and human cells.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Takuya Matsumoto, Tomoki Osawa, Hikaru Taniguchi, Akira Saito, Ryosuke Yamada, Hiroyasu Ogino
Summary: This study focused on utilizing accumulated intermediates, including acetyl-CoA, in mitochondria to enhance carotenoid production in Saccharomyces cerevisiae. By localizing carotenoid-producing enzymes in the mitochondria, the strain showed improved carotenoid production compared to the strain with enzymes in the cytosol. Furthermore, increasing glucose concentration improved cell growth and carotenoid production in mitochondria, but not in the cytosol.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Till Klecker, Benedikt Westermann
Summary: Mitochondria are complex organelles with two membranes, characterized by characteristic folds of the inner membrane called cristae. Recent studies have identified four major pathways that cooperate to shape cristae membranes, including ATP synthase dimer formation, assembly of MICOS, inner membrane remodelling by GTPase, and modulation of mitochondrial lipid composition. These pathways play a crucial role in establishing mitochondrial membrane architecture.
Article
Biochemistry & Molecular Biology
Justyna Zitek, Martin S. King, Priscila Pen, Eva Pyrihov, Alannah C. King, Edmund R. S. Kunji, Vladimir Hampl
Summary: Paratrimastix pyriformis is a free-living flagellate belonging to the group Metamonada and it has a mitochondrion-related organelle involved in one-carbon folate metabolism. The organelle contains solute carrier family 25 (SLC25) members responsible for metabolite exchange across the mitochondrial inner membrane. PpMC1, the adenine nucleotide carrier, was shown to transport ATP, ADP, and to a lesser extent, AMP, but not phosphate. It represents a distinct class of adenine nucleotide carriers different from ADP/ATP carriers and ATP-Mg/phosphate carriers.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Cell Biology
Anna Santarsiero, Paolo Convertini, Antonio Vassallo, Valentina Santoro, Simona Todisco, Dominga Iacobazzi, Yvonne Fondufe-Mittendorf, Giuseppe Martelli, Marcos R. de Oliveira, Rosangela Montanaro, Vincenzo Brancaleone, Johannes Stockl, Vittoria Infantino
Summary: Phenolic compounds in red wine powder show potential immunomodulatory and anti-inflammatory effects by reducing proinflammatory cytokines secretion, restoring Annexin A1 levels, lowering NF-kappa B activity, and regulating metabolic genes involved in the citrate pathway, ultimately leading to suppression of inflammatory pathways and activation of proresolutive processes in macrophages.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Endocrinology & Metabolism
Maher A. Shahrour, Francesco M. Lasorsa, Vito Porcelli, Imad Dweikat, Maria Antonietta Di Noia, Michal Gur, Giulia Agostino, Avraham Shaag, Teresa Rinaldi, Giuseppe Gasparre, Flora Guerra, Alessandra Castegna, Simona Todisco, Bassam Abu-Libdeh, Orly Elpeleg, Luigi Palmieri
Summary: This study reports a novel genetic mutation associated with hyperinsulinism/hyperammonemia syndrome, which leads to loss of protein function. The mutation results in a significant reduction in mitochondrial GTP content, leading to hyperactivation of glutamate dehydrogenase. These findings not only reveal the underlying genetic etiology of hyperinsulinism/hyperammonemia syndrome but also expand the role of mitochondrial transporters in insulin secretion.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Simona Feno, Fabio Munari, Denis Vecellio Reane, Rosanna Gissi, Dieu-Huong Hoang, Alessandra Castegna, Benedicte Chazaud, Antonella Viola, Rosario Rizzuto, Anna Raffaello
Summary: Research shows that the negative regulatory subunit MCUb of the mitochondrial calcium uniporter (MCU) complex promotes muscle regeneration by controlling macrophage responses. Macrophages lacking MCUb are unable to efficiently acquire the anti-inflammatory profile, leading to delayed regeneration and exhaustion of the satellite cell pool. The study highlights the importance of tightly controlling MCUb abundance in macrophages to enable functional differentiation of satellite cells and restoration of tissue homeostasis after damage.
Article
Medicine, General & Internal
Vincenzo Tragni, Francesca Preziusi, Luna Laera, Angelo Onofrio, Ivan Mercurio, Simona Todisco, Mariateresa Volpicella, Anna De Grassi, Ciro Leonardo Pierri
Summary: This study investigates the interaction energy between newly emerged SARS-CoV-2 variants and the human ACE2 receptor through computational analysis. The results suggest that certain variants may have higher transmissibility, which aids in predicting the spread of new variants sequenced from future patients and implementing appropriate preventive measures.
Review
Biochemistry & Molecular Biology
Federica Marra, Paola Lunetti, Rosita Curcio, Francesco Massimo Lasorsa, Loredana Capobianco, Vito Porcelli, Vincenza Dolce, Giuseppe Fiermonte, Pasquale Scarcia
Summary: Neuromuscular diseases (NMDs) involve skeletal muscle dysfunction due to genetic mutations, leading to muscle weakness and fatigue. Mitochondrial dysfunction plays a key role in the development of NMDs, with alterations in genes and pathways related to mitochondrial function contributing to the disease progression. Diagnosis and treatment strategies for NMDs focus on identifying genetic mutations and mitochondrial pathways involved in the development of the disease.
Article
Biochemistry & Molecular Biology
Michela Marsico, Anna Santarsiero, Ilaria Pappalardo, Paolo Convertini, Lucia Chiummiento, Alessandra Sardone, Maria Antonietta Di Noia, Vittoria Infantino, Simona Todisco
Summary: Metabolic reprogramming is crucial for cancer cells to meet high energy needs and maintain redox balance, with glutamine metabolism playing a key role. In the study of hepatocellular carcinoma, up-regulation of GDH1 was observed, and inhibition of this enzyme showed promising results in reducing cell proliferation and identifying potential inhibitors. These findings suggest that targeting hGDH1 could be a viable strategy to disrupt the metabolic reprogramming of HCC cells.
Review
Biology
Simona Todisco, Anna Santarsiero, Paolo Convertini, Giulio De Stefano, Michele Gilio, Vito Iacobazzi, Vittoria Infantino
Summary: Non-alcoholic steatohepatitis (NASH) is a serious transformation from simple steatosis to inflammation and hepatic injury. Profound metabolic dysregulations are associated with NASH, and the peroxisomal-proliferator-activated receptor alpha (PPAR alpha) plays a key role in its pathogenesis. However, many aspects of NASH have not yet been fully clarified.
Article
Nutrition & Dietetics
Bigna K. Bolsterli, Eugen Boltshauser, Luigi Palmieri, Johannes Spenger, Michaela Brunner-Krainz, Felix Distelmaier, Peter Freisinger, Tobias Geis, Andrea L. Gropman, Johannes Haberle, Julia Hentschel, Bruno Jeandidier, Daniela Karall, Boris Keren, Annick Klabunde-Cherwon, Vassiliki Konstantopoulou, Raimund Kottke, Francesco M. Lasorsa, Christine Makowski, Cyril Mignot, Ruth O'Gorman Tuura, Vito Porcelli, Rene Santer, Kuntal Sen, Katja Steinbruecker, Steffen Syrbe, Matias Wagner, Andreas Ziegler, Thomas Zoeggeler, Johannes A. Mayr, Holger Prokisch, Saskia B. Wortmann
Summary: Defects in the mitochondrial malate aspartate shuttle system and mitochondrial pyruvate carrier 1 are associated with neurological phenotypes and hepatopathic-neuropsychiatric phenotypes. Ketogenic diets and carbohydrate-restricted/fat-enriched diets are effective interventions. Early (genetic) diagnosis is crucial for initiating proper treatment.
Article
Pharmacology & Pharmacy
Simona Todisco, Biagia Musio, Vito Pesce, Maria Maddalena Cavalluzzi, Giuseppe Petrosillo, Gianluigi La Piana, Maria Noemi Sgobba, Nikola Schlosserova, Lucas Cafferati Beltrame, Rosa Di Lorenzo, Vincenzo Tragni, Domenico Marzulli, Lorenzo Guerra, Anna De Grassi, Vito Gallo, Mariateresa Volpicella, Luigi Leonardo Palese, Giovanni Lentini, Ciro Leonardo Pierri
Summary: Mitochondria and mitochondrial proteins are promising pharmacological target candidates for the prevention of hypertension and cardiovascular diseases. Targeting mitochondrial dysfunction in cardiomyocytes can prevent heart failure. Advances in omics approaches and computational methods allow for detailed investigation of impaired mitochondrial pathways in CVDs and the design of new selective drugs.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anna Santarsiero, Ilaria Pappalardo, Gabriella Margherita Rosa, Isabella Pisano, Stefano Superchi, Paolo Convertini, Simona Todisco, Patrizia Scafato, Vittoria Infantino
Summary: Hepatocellular carcinoma (HCC) is the most common type of liver cancer and a significant cause of cancer-related deaths. In this study, a newly synthesized chalcone ETTC showed cytotoxic activity and induced apoptosis in HCC cells through the activation of the intrinsic pathway of apoptosis and mitochondrial targeting. These findings suggest the potential antitumor effect of ETTC in HCC.
Meeting Abstract
Medicine, General & Internal
L. Palmieri, M. A. Shahroor, F. M. Lasorsa, I. Dweikat, V. Porcelli, M. Gur, M. A. Di Noia, A. Shaag, B. Abu-Libdeh, O. Elpeleg
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2022)
Meeting Abstract
Medicine, General & Internal
S. N. Barile, M. C. Magnifico, L. Palmieri, F. Distelmaier, L. Viggiano, A. Pignataro, S. Petralla, J. Hentschel, V. Porcelli, E. Poeta, I. Pisano, G. Fiermonte, S. A. Anderson, B. Monti, F. M. Lasorsa
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Neurosciences
Eleonora Poeta, Sabrina Petralla, Giorgia Babini, Brunaldo Renzi, Luigi Celauro, Maria Chiara Magnifico, Simona Nicole Barile, Martina Masotti, Francesca De Chirico, Francesca Massenzio, Luigi Viggiano, Luigi Palmieri, Marco Virgili, Francesco Massimo Lasorsa, Barbara Monti
Summary: This study investigates the molecular and epigenetic mechanisms of histone acetylation dysfunctions in AGC1 deficiency. The results suggest that curcumin inhibits OPC proliferation and promotes differentiation, while suberanilohydroxamic acid has limited effects on proliferation but stimulates OPC differentiation. These findings contribute to understanding OPC and NPC proliferation/differentiation and identifying potential therapeutic targets for AGC1 deficiency and other white matter neuropathologies.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Maria De Luca, Ilaria Pappalardo, Antonina Rita Limongi, Emanuele Viviano, Rosa Paola Radice, Simona Todisco, Giuseppe Martelli, Vittoria Infantino, Antonio Vassallo
Summary: In recent years, there has been a growing interest in using microalgal lipids in various industries such as food, chemical, pharmaceutical, and cosmetic. Microalgae are considered oleaginous species and are valued for their moisturizing, emollient, and softening properties in cosmetics. Despite the high costs associated with commercialization, the potential of using microalgae for different industrial purposes has attracted increasing attention from scientists.
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)