Article
Immunology
Shrey A. Shah, Robert S. Oakes, Senta M. Kapnick, Christopher M. Jewell
Summary: Microneedles, a new biomaterials technology, have the potential to deliver vaccines and immunotherapies efficiently and painlessly to the skin's specialized immune niche. Understanding the immunological and mechanical properties of microneedles is crucial for developing new microneedle-based vaccines and immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Nitzan Letko Khait, Eric Ho, Molly S. Shoichet
Summary: Stroke is a common cause of disability, with no current effective treatment available. Innovative clinical trials to improve stroke recovery often face challenges due to the complexity of stroke pathophysiology. While immunomodulatory strategies have shown success in experimental studies, translating them into clinical practice remains difficult.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Engineering, Biomedical
Shreya S. Soni, Christopher B. Rodell
Summary: Biomaterials serve as complex engineered tools for interacting with biological systems and aiding in the treatment of diseases. The immune response to polymeric materials is crucial in governing how the body reacts to biomaterial implants and drug delivery systems. Understanding molecular interactions and bulk biophysical properties is key in designing biomaterials that drive immune responses.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Hunter Newman, Yuru Vernon Shih, Shyni Varghese
Summary: Impaired bone healing affects 5-10% of cases after injury, with timely resolution of inflammation being crucial for normal healing. Modulating the initial inflammatory phase and promoting its resolution can create a pro-regenerative environment for bone regeneration.
Review
Chemistry, Multidisciplinary
Xi Wang, Natalie K. Brown, Bo Wang, Kaavian Shariati, Kai Wang, Stephanie Fuchs, Juan M. Melero-Martin, Minglin Ma
Summary: Islet transplantation holds promise as a curative therapy for T1D, but faces challenges like systemic immunosuppression and limited islet viability. Inducing local immunotolerance shows potential to enhance graft function, broaden therapy applicability, and reduce adverse effects of systemic immunosuppression. Interdisciplinary research involving cellular engineering, immunotherapy, and novel biomaterials is driving the development of new combinatorial strategies for the benefit of the entire T1D population.
Review
Engineering, Biomedical
Mojtaba Farahani, Abbas Shafiee
Summary: The universal increase in the number of patients with nonhealing skin wounds imposes a huge social and economic burden. The recent advances in materials and fabrication technologies have led to the development of smart wound dressings, such as 3D-printed dressings and biomolecule-loaded dressings, which accelerate wound healing and provide effective wound care management strategies.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Engineering, Biomedical
Gerry L. Koons, Panayiotis D. Kontoyiannis, Mani Diba, Letitia K. Chim, David W. Scott, Antonios G. Mikos
Summary: This study investigated the effect of different printing temperatures on the bioactivity of BMP-2 in 3D printed constructs. The results showed that constructs printed at physiological and intermediate temperatures exhibited similar bioactive BMP-2 release as non-printed particulate delivery vehicles, while elevated printing temperature led to decreased bioactive BMP-2 release. These findings provide insights into the impact of elevated printing temperatures on BMP-2 bioactivity during extrusion-based 3D printing and guide material selection for 3D printing with growth factors.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Aisha Rauf, Tanveer A. Tabish, Ibrahim M. Ibrahim, Muhammad Rauf ul Hassan, Sabira Tahseen, Mansoor Abdullah Sandhu, Gul Shahnaz, Abbas Rahdar, Magali Cucchiarini, Sadanand Pandey
Summary: The preactivated thiolated chitosan nanoparticles show enhanced in vivo oral efficacy and hepatoprotective effects for tuberculosis treatment, with significantly improved permeation and oral bioavailability compared to conventional formulations. Additionally, the nanoparticles provide immunomodulation and improve liver function through modulation of gene expression levels and markers of oxidative stress.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Lin Liu, Haifeng Tang, Yanjun Wang
Summary: Polymeric biomaterials have great potential for drug delivery systems (DDS) in the field of osteoarthritis (OA) therapy. This review summarizes recent advances in polymeric DDS for OA therapy, including an overview of OA pathophysiology, different categories of polymeric DDS, and the delivery of bioactive agents. Prospective directions for advancing polymeric DDS are also highlighted.
Review
Pharmacology & Pharmacy
Paula de Freitas Rosa Remiro, Mariana Harue Taniguchi Nagahara, Rafael Abboud Azoubel, Michelle Franz-Montan, Marcos Akira d'avila, Angela Maria Moraes
Summary: There are various routes of drug administration, each with its own advantages and limitations. Topical application in the oral cavity offers benefits such as painlessness, non-invasiveness, easy application, and the ability to bypass drug swallowing. The oral mucosa has high permeability, allowing rapid delivery of medication to the bloodstream without harming the stomach. This drug delivery route is gaining significant interest in the pharmaceutical industry. This review provides a condensed overview of biomaterials, polymers, production methods, and market perspectives for topical drug delivery in the oral cavity.
Article
Engineering, Biomedical
Grace G. Bushnell, Sophia M. Orbach, Jeffrey A. Ma, Howard C. Crawford, X. S. Wicha, Jacqueline S. Jeruss, Lonnie D. Shea
Summary: Pancreatic cancer has the worst prognosis among all cancers due to its aggressiveness and lack of early detection methods. A biomaterial scaffold developed has shown potential to recruit tumor cells and reflect immune dysregulation in spontaneous tumor models, particularly in identifying early stages of aggressive pancreatic cancer.
Review
Engineering, Biomedical
Ni Su, Cassandra Villicana, Fan Yang
Summary: This review discusses the progress in immunomodulation research for bone applications using biomaterials and cell-based strategies, with a focus on different disease types. The role of the immune system in orthopedic applications, including prolonging orthopaedic implant lifetime and enhancing fracture healing, is reviewed. Recent research progress in harnessing immunomodulatory strategies for regenerating critical-sized, long bone or cranial bone defects, and treating osteolytic bone diseases is also discussed.
Review
Engineering, Biomedical
Parisa Heydari, Mahshid Kharaziha, Jaleh Varshosaz, Shaghayegh Haghjooy Javanmard
Summary: The immune system plays a critical role in orchestrating the wound healing process, and can be optimized using biomaterials, bioactive molecules, and cell delivery. Strategies for immunomodulation in chronic wound healing include investigating the interaction between the extracellular mechanism and immune cells, biomaterial-based strategies, and the release of bioactive agents and stem cell therapy.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Fu Wei, Shuyun Liu, Mingxue Chen, Guangzhao Tian, Kangkang Zha, Zhen Yang, Shuangpeng Jiang, Muzhe Li, Xiang Sui, Zhiwei Chen, Quanyi Guo
Summary: Understanding the immunogenicity of biomaterials and the host response is crucial for guiding the constructive remolding process of damaged tissue. Researchers hope to promote cartilage repair and regeneration by controlling immune components of biomaterials and modulating the immune system.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Amir Erfani, Jeremy M. Schieferstein, Paul Reichert, Chakravarthy N. Narasimhan, Cinthia Pastuskovas, Vaishali Parab, Denarra Simmons, Xiaoyu Yang, Apoorv Shanker, Paula Hammond, Patrick S. Doyle
Summary: This work presents a platform technology that combines the stability of crystalline antibodies with injectability and tunability of soft hydrogel particles for subcutaneous administration of monoclonal antibodies (mAbs). The composite alginate hydrogel particles, generated via a gentle centrifugal encapsulation process, achieve stable, high concentration, and injectable formulations of the mAbs. The study also evaluates the biocompatibility and pharmacokinetics of the crystalline antibody-laden alginate hydrogel particles in animal models.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Polymer Science
Xiaolei Zhang, Xia Zhao, Jittima Amie Luckanagul, Jing Yan, Yuzhe Nie, L. Andrew Lee, Qian Wang
Article
Biotechnology & Applied Microbiology
Xiaolei Zhang, Pongkwan Sitasuwan, Gary Horvath, Jia Yang, Yuzhe Nie, Margarita Marinova, L. Andrew Lee, Qian Wang
ENZYME AND MICROBIAL TECHNOLOGY
(2018)
Article
Chemistry, Analytical
Pongkwan Sitasuwan, Cathleen Melendez, Margarita Marinova, Michelle Spruill, L. Andrew Lee
JOURNAL OF ANALYTICAL TOXICOLOGY
(2019)
Article
Biochemical Research Methods
B. Todd Mullis, Sunil Hwang, L. Andrew Lee, Anton Iliuk, Rebekah Woolsey, David Quilici, Qian Wang
Article
Biochemical Research Methods
Patrick A. Kates, John J. Tomashek, David A. Miles, L. Andrew Lee
Article
Biotechnology & Applied Microbiology
Caleb R. Schlachter, Amanda C. McGee, Pongkwan N. Sitasuwan, Gary C. Horvath, Nanda G. Karri, L. Andrew Lee, John J. Tomashek
Summary: Researchers improved the activity of β-GUS on drug metabolites by designing enzyme chimeras and site-saturation variants, and discovered a chimera from enzymes of different species that exhibited a significant increase in activity on morphine-3-glucuronide.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Chemistry, Analytical
L. Andrew Lee, Amanda C. McGee, Pongkwan Sitasuwan, John J. Tomashek, Chris Riley, Ana Celia Munoz-Munoz, Lawrence Andrade
Summary: Different enzymes exhibit varying performances in clinical urine specimens, showing distinct substrate preferences and pH optima. Urine-specific properties affect the hydrolytic effectiveness of beta-glucuronidases, with some enzymes being more significantly impacted. Diluting urine with buffer greatly improves achieving target pH and reduces the impact of endogenous compounds on enzyme performance.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2022)
Review
Microbiology
Shalini Iyer, Rajesh Yadav, Smriti Agarwal, Shashank Tripathi, Rachit Agarwal
Summary: Researchers have used bioengineering techniques to synthesize vaccines against respiratory viral pathogens. These vaccines enhance immunogenicity by controlling their physicochemical properties and have shown promising results in treating diseases such as COVID-19. The feasibility of using materials such as polymeric particles, liposomes, and virus-like particles for vaccine development has been explored.
CLINICAL MICROBIOLOGY REVIEWS
(2022)
Article
Materials Science, Biomaterials
Pallavi Raj Sharma, Ameya Atul Dravid, Yeswanth Chakravarthy Kalapala, Vishal K. Gupta, Sharumathi Jeyasankar, Avijit Goswami, Rachit Agarwal
Summary: Inhalable microparticle-based drug delivery platforms are being extensively studied for Tuberculosis treatment. The physical parameters of microcarriers and their interaction with Mycobacterium tuberculosis infected cells have been explored in this study. The results show that cationic particles improve the uptake of microparticles by infected cells and enhance the anti-bacterial function of the drug.
BIOMATERIALS ADVANCES
(2022)
Article
Biochemical Research Methods
Yuko Ogata, Pamela M. Quizon, Nancy S. Nightlinger, Pongkwan Sitasuwan, Casey Snodgrass, L. Andrew Lee, Jeffrey D. Meyer, Richard S. Rogers
Summary: The study presented a fully automated MAM sample preparation protocol, achieving rapid desalting using miniaturized size-exclusion chromatography columns on an automated liquid handler. Compared to manual preparation, this automated method showed no significant change in product attributes and their quantities, while enhancing sample recovery and reproducibility.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Biotechnology & Applied Microbiology
Kaamini M. Dhanabalan, Ameya A. Dravid, Smriti Agarwal, Ramanath K. Sharath, Ashok Kumar Padmanabhan, Rachit Agarwal
Summary: Trauma to the knee joint can lead to cartilage degeneration and bone erosion, causing osteoarthritis. Current treatments focus on symptom relief. Modulating autophagy and targeting senescence pathways show potential as treatment strategies. A study found that rapamycin-loaded microparticles can effectively mitigate cartilage damage and inflammation, potentially preventing the progression of post-traumatic osteoarthritis.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Ameya A. Dravid, Kaamini Dhanabalan, Smriti Agarwal, Rachit Agarwal
Summary: A nanoliposomal formulation loaded with RvD1 has been developed to target and resolve osteoarthritis-associated inflammation. This controlled-release formulation effectively treats OA and relieves pain by inhibiting inflammatory activity and recruiting M2 macrophages, preventing cartilage damage and osteophyte formation.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Caleb R. Schlachter, Andrea O'Malley, Linda L. Grimes, John J. Tomashek, Maksymilian Chruszcz, L. Andrew Lee
Summary: Sulfatases are enzymes that hydrolyze sulfate from sulfated organic substrates. They can be used in biotechnology to analyze sulfated metabolites in humans. This study focused on the characterization of a sulfatase from Pedobacter yulinensis and found new activities that are advantageous for biotechnological applications.
Article
Medicine, Research & Experimental
Pongkwan Sitasuwan, Thomas W. Powers, Tiffany Medwid, Yuting Huang, Bradley Bare, L. Andrew Lee
Summary: The multi-attribute method (MAM) is a recent advancement in liquid chromatography-mass spectrometry for simultaneous monitoring of multiple product quality attributes in the pharmaceutical industry. While MAM offers automated data processing and reporting, its sample preparation remains cumbersome and low throughput.
Article
Materials Science, Biomaterials
Kaamini M. Dhanabalan, Vishal K. Gupta, Rachit Agarwal
BIOMATERIALS SCIENCE
(2020)