Article
Microbiology
Jimena Rinaldi, Ignacio Fernandez, Heewhan Shin, Gabriela Sycz, Semini Gunawardana, Indika Kumarapperuma, Juan M. Paz, Lisandro H. Otero, Maria L. Cerutti, Angeles Zorreguieta, Zhong Ren, Sebastian Klinke, Xiaojing Yang, Fernando A. Goldbaum
Summary: This study presents the crystal structure of a light-sensitive histidine kinase in the pathogenic bacterium Brucella abortus, revealing the mechanism of protein structural changes in response to different signaling states. Direct comparisons between the full-length and truncated constructs show concerted protein motions in an asymmetric dimer framework in response to light signals. These findings provide mechanistic insights into modular sensory proteins with similar modular architectures.
Article
Biochemistry & Molecular Biology
Celine Bournonville, Kentaro Mori, Paul Deslous, Guillaume Decros, Tim Blomeier, Jean-Philippe Mauxion, Joana Jorly, Stephanie Gadin, Cedric Cassan, Mickael Maucourt, Daniel Just, Cecile Bres, Christophe Rothan, Carine Ferrand, Lucie Fernandez-Lochu, Laure Bataille, Kenji Miura, Laure Beven, Matias D. Zurbriggen, Pierre Petriacq, Yves Gibon, Pierre Baldet
Summary: Mutation in the PAS/LOV protein gene in tomato plants leads to ascorbate-enriched fruits, indicating the light-dependent regulation of ascorbate metabolism. Blue light modulates the interaction between PLP and GGP, resulting in increased synthesis of ascorbate.
Article
Biochemistry & Molecular Biology
Kosei Shibata, Yusuke Nakasone, Masahide Terazima
Summary: The blue light-regulated phosphodiesterase BlrP1 from Klebsiella pneumoniae functions as a nonlinear light intensity sensor, with its turnover number of the hydrolysis reaction being proportional to the fraction of the excited protein squared. This suggests that BlrP1 is capable of sensing a strong light environment.
Article
Horticulture
Hui Li, Yibo Bai, Yaxin Yang, Huan Zheng, Xianbin Xu, Haoran Li, Wu Wang, Jianmin Tao
Summary: Teinturier grapevine cultivars, characterized by red pulp berries, showed inhibited anthocyanin accumulation under bagging treatment, while rapid accumulation of malvidin, cyanidin, and delphinidin derivatives was observed after exposure to light. Transcriptomic analysis revealed that genes associated with light-responsive factors, transcription factors, and structural genes were significantly up-regulated under bag removal treatment. These findings provide insights into the molecular mechanism of light-induced anthocyanin biosynthesis in teinturier grapes.
SCIENTIA HORTICULTURAE
(2023)
Article
Biochemistry & Molecular Biology
Tiedong Liu, Xiwen Zhang
Summary: This study found that blue light significantly affects the formation of maize stomata, and identified key genes and signaling pathways involved in this process through transcriptome and metabolomics analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Amos Samkumar, Dan Jones, Katja Karppinen, Andrew P. Dare, Nina Sipari, Richard V. Espley, Inger Martinussen, Laura Jaakola
Summary: This study found that both red and blue light can enhance the biosynthesis of anthocyanins in bilberry fruit, with red light showing a more significant effect in increasing anthocyanin concentration. Transcriptomic analysis revealed up-regulation of major anthocyanin structural genes, key regulatory MYB transcription factors, and abscisic acid biosynthetic genes under both light treatments.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Optics
Li Song, Xiwei Guo, Yuan Liu, Yu Wen, Yingze Ren, Chang Tan, Meng Zhang, Hao Xu, Xiaoyang Guo, Lishuang Wang
Summary: A synergistic regulation strategy through interface inducing and polymer spatial confinement has been used to achieve blue emission in metal halide perovskite. The improved polymer blend can inhibit the coalescence and ripening of the crystals while passivating the defects. As a result, the dual-polymer regulated blue perovskite light-emitting devices (PeLEDs) achieved an external quantum efficiency (EQE) of 3.73% and a maximum brightness of 7051 cd/m2. This facile method may provide alternative approaches to construct efficient blue PeLEDs.
JOURNAL OF LUMINESCENCE
(2023)
Article
Microbiology
Yifan Li, Tingting Sun, Degang Guo, Jia Gao, Jian Zhang, Feng Cai, Reinhard Fischer, Qirong Shen, Zhenzhong Yu
Summary: In Trichoderma guizhouense, the blue light receptor BLR1 dominates light responses while ENV1 is responsible for photoadaptation. The core components of the light signaling network, BLR1 and HOG1, control the expression of a large portion of the differentially expressed genes (DEGs). This study reveals a regulatory network based on blue light receptors and the MAPK HOG pathway for various processes in T. guizhouense, including conidiation and hydrophobin production.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ioana-Alexandra Bala, Naomi Tritean, Alin Alexandru Enache, Bogdan Trica, Diana Constantinescu-Aruxandei, Florin Oancea
Summary: This study investigates the effects of blue light irradiation on Trichoderma atroviride, including cellulase and protease activities, conidia formation, dimension and behavior, and chlamydospore abundance. The results show varying effects on enzymatic activities, increased conidia number and dimensions, and a possible hydrophobic character induced by high dose irradiation.
APPLIED SCIENCES-BASEL
(2023)
Article
Genetics & Heredity
Zhenzhong Yu, Christian Streng, Ramon F. Seibeld, Olumuyiwa A. Igbalajobi, Kai Leister, Julian Ingelfinger, Reinhard Fischer
Summary: Fungi sense light of different wavelengths using blue-, green-, and red-light photoreceptors. While many genes in the model fungus Aspergillus nidulans are under light control, regulated through phytochrome and respond to specific wavelengths, there are also alternative red-light sensing systems and blue-light induced genes that may involve cryptochrome and phytochrome in the regulatory process. Phytochrome is essential for the response to blue light and plays a role in both blue- and red-light sensing pathways.
Article
Developmental Biology
Samantha J. England, Amber K. Rusnock, Amra Mujcic, Angelica Kowalchuk, Sarah de Jager, William C. Hilinski, Jose L. Juarez-Morales, Matthew E. Smith, Ginny Grieb, Santanu Banerjee, Katharine E. Lewis
Summary: This study reveals two molecularly distinct subsets of zebrafish V0v spinal interneurons, as well as multiple transcriptional regulators that may act downstream of Evx1/2 to specify the essential functional characteristics of these cells. The findings suggest that in the absence of Evx1 and Evx2, V0v spinal interneurons may change their neurotransmitter phenotypes and express markers of distinct inhibitory spinal interneurons or motoneurons.
NEURAL DEVELOPMENT
(2023)
Article
Immunology
Ming Liu, Keman Xu, Fatma Saaoud, Ying Shao, Ruijing Zhang, Yifan Lu, Yu Sun, Charles Drummer, Li Li, Sheng Wu, Satya P. Kunapuli, Gerard J. Criner, Jianxin Sun, Huimin Shan, Xiaohua Jiang, Hong Wang, Xiaofeng Yang
Summary: By mining a database of 102 transcriptomic datasets, we have identified significant variations in the expressions of m(6)A-RNA methylation regulators in various diseases and cancers, providing new insights into potential therapeutic targets for these conditions.
JOURNAL OF IMMUNOLOGY RESEARCH
(2022)
Article
Cell Biology
Shu Anami, Takafumi Yamashino, Ryo Suzuki, Kota Nakai, Kensuke Sato, Bowen Wu, Masashi Ryo, Mamoru Sugita, Setsuyuki Aoki
Summary: The study focused on the subcellular patterns of localization and interaction between PHKs and HPts in the moss Physcomitrella patens. It was found that PHKs are mainly localized and interact with HPts in the nucleus, which is a unique feature for plant HKs. Additionally, red light triggered interactions between PHKs and HPts in the cytoplasm, and light repressed the expression of certain genes.
Article
Plant Sciences
J. M. Xu, Y. Liu, M. X. Liu, Z. G. Xu
Summary: The application of red and blue light from LEDs can affect the growth, physiology, and proteome of potato plantlets in vitro culture. Blue light increases leaf thickness, while red and blue light treatments result in changes in photosynthetic rate, soluble sugar content, and starch content. The difference in growth performances of the plantlets under different light treatments is related to altered levels of plant hormones. Furthermore, the differentially expressed proteins identified play important roles in various metabolic pathways. Overall, these findings provide insights into the effects of different types of light on potato growth and offer potential applications for in vitro propagation.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Developmental Biology
Anna Reade, Laura B. Motta-Mena, Kevin H. Gardner, Didier Y. Stainier, Orion D. Weiner, Stephanie Woo
Review
Chemistry, Multidisciplinary
Aba Losi, Kevin H. Gardner, Andreas Moeglich
Article
Biochemistry & Molecular Biology
Lindsay Clark, Igor Dikiy, Daniel M. Rosenbaum, Kevin H. Gardner
JOURNAL OF BIOMOLECULAR NMR
(2018)
Article
Multidisciplinary Sciences
Spencer T. Glantz, Erin E. Berlew, Zaynab Jaber, Benjamin S. Schuster, Kevin H. Gardner, Brian Y. Chow
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Igor Dikiy, Uthama R. Edupuganti, Rinat R. Abzalimov, Peter P. Borbat, Madhur Srivastava, Jack H. Freed, Kevin H. Gardner
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Editorial Material
Biochemistry & Molecular Biology
Igor Dikiy, Kevin H. Gardner
MOLECULAR MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
James N. Iuliano, Jinnette Tolentino Collado, Agnieszka A. Gil, Pavithran T. Ravindran, Andras Lukacs, SeungYoun Shin, Helena A. Woroniecka, Katrin Adamczyk, James M. Aramini, Uthama R. Edupuganti, Christopher R. Hall, Gregory M. Greetham, Igor Sazanovich, Ian P. Clark, Taraneh Daryaee, Jared E. Toettcher, Jarrod B. French, Kevin H. Gardner, Carlos L. Simmerling, Stephen R. Meech, Peter J. Tonge
ACS CHEMICAL BIOLOGY
(2020)
Article
Biophysics
Xingjian Xu, Donald Gagne, James M. Aramini, Kevin H. Gardner
Summary: Proteins often undergo conformational interconversion critical to their function, with pressure being a potential route to control these transitions. Research on ARNT PAS-B Y456T demonstrates that the interconversion process supports two key hypotheses: the slipped conformation is smaller and less compressible, and the process proceeds through a chiefly unfolded intermediate state.
BIOPHYSICAL JOURNAL
(2021)
Article
Developmental Biology
Jesselynn LaBelle, Adela Ramos-Martinez, Kyle Shen, Laura B. Motta-Mena, Kevin H. Gardner, Stefan C. Materna, Stephanie Woo
Summary: The improved optogenetic gene expression system TAEL 2.0 allows for more efficient induction of gene expression and finer spatiotemporal control in zebrafish. By creating functional stable transgenic lines, it enables induction of expression at late embryonic and larval stages. This enhanced system will be a broadly useful resource for the zebrafish community.
Editorial Material
Biochemistry & Molecular Biology
James J. Siclari, Kevin H. Gardner
Summary: In this study, insights into the binding mechanism of artificial agonists to the PPARg nuclear receptor were presented, supporting a two-step model with induced fit and conformational selection aspects. This mechanism may have implications for drug development in related receptors.
Article
Biochemical Research Methods
Erin E. Berlew, Keisuke Yamada, Ivan A. Kuznetsov, Eleanor A. Rand, Chandler C. Ochs, Zaynab Jaber, Kevin H. Gardner, Brian Y. Chow
Summary: In this study, we efficiently created single-component optogenetic tools for membrane recruitment-based signaling perturbation using BcLOV4 technology. Screening a limited set of genetic constructs and observing their dynamic translocation in response to light enabled the identification of viable signaling control tools. We successfully created an optogenetic Cdc42 GTPase and Rac1-activating Tiam1 GEF protein, completing a toolbox for spatiotemporally precise induction of Rho-family GTPase signaling.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jaynee E. Hart, Kevin H. Gardner
Summary: Phototropins are light-activated kinases crucial for plant physiology and optogenetic tools. Recent studies have made progress in understanding the structure and mechanism of phototropins, providing models and insights into their regulation. This information is essential for advancing fundamental understanding of plant physiology and enabling engineering efforts.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Donald Gagne, Kevin H. Gardner, Donald Gagne, James M. Aramini, Bruce A. Johnson, Kevin H. Gardner
Meeting Abstract
Biochemistry & Molecular Biology
Zaynab Jaber, Spencer Glantz, Erin Berlew, Brian Y. Chow, Kevin H. Gardner
Meeting Abstract
Biochemistry & Molecular Biology
Igor Dikiy, Uthama Edupuganti, Rinat Abzalimov, Peter Borbat, Jack Freed, Kevin Gardner