Article
Microbiology
Christiane Ribeiro Janner Herrera, Vanessa Rodrigues Vieira, Tiago Benoliel, Clara Vida Galrao Correa Carneiro, Janice Lisboa De Marco, Lidia Maria Pepe de Moraes, Joao Ricardo Moreira de Almeida, Fernando Araripe Goncalves Torres
Summary: Sugarcane bagasse, a rich source of xylose, was utilized to produce xylonic acid with engineered Zymomonas mobilis. Expression of XDH gene from Paraburkholderia xenovorans led to the highest production, with deletion of xylose reductase gene further increasing yields. The findings suggest Z. mobilis could serve as a potential platform for organic acid production from cheap lignocellulosic biomass in biorefineries.
Article
Biology
Reinis Rutkis, Inese Strazdina, Zane Lasa, Per Bruheim, Uldis Kalnenieks
Summary: A mutant strain with partly inactivated Pdc showed increased pyruvate production compared to its parent strain, along with increased acetaldehyde production despite similar ADH activity. Simulation with the kinetic model of Z. mobilis E-D pathway suggested a higher ratio between respiratory NADH oxidase and ADH activities is needed for the observed acetaldehyde to ethanol production ratio in the mutant. This finding has implications for the directionality of ADH isoenzyme operation in vivo and interactions between ADH and Pdc.
BMC RESEARCH NOTES
(2021)
Article
Biochemistry & Molecular Biology
Kun Zhang, Wenwen Zhang, Mengxing Qin, Yi Li, Hailei Wang
Summary: This study investigates the role of ZMO0293 in Z. mobilis using gene deletion and heterologous expression methods. The results show that deletion of ZMO0293 gene leads to slowed growth, reduced ethanol production, and decreased activities of key enzymes involved in glucose metabolism in the presence of high concentrations of glucose. Furthermore, ZMO0293 deletion causes transcriptional changes in some genes of the Entner Doudoroff pathway in the ZM4-Delta ZM0293 strain. This study reveals the function of ZMO0293 gene and provides a new biological part for synthetic biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Food Science & Technology
Lu Liu, Jian-Ting Li, Sheng-Hao Li, Lin-Pei Liu, Bo Wu, Yan-Wei Wang, Shi-Hui Yang, Cheng-Han Chen, Fu-Rong Tan, Ming-Xiong He
Summary: This review summarizes the physiological characteristics and advantages of Z. mobilis in the food industry for the first time, and further elaborates its research status in the food industry in terms of food additive synthesis, fermentation applications, and prebiotic efficacy, providing a theoretical basis for its development and application in the food industry. The review also discusses the shortcomings of its practical applications in the current food industry and explores other ways to expand the applications of Z. mobilis in food processing.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Agricultural Engineering
Yuqi Li, Yao Xu, Yihan Xue, Shihui Yang, Yanfen Cheng, Weiyun Zhu
Summary: A novel integrated process for ethanol production from lignocellulosic biomass is reported. The process involves simultaneous pretreatment, saccharification, and fermentation using environmentally friendly technologies. The conversion from lignocellulosic biomass to ethanol was completed in four days with the potential for producing other metabolites.
BIOMASS & BIOENERGY
(2022)
Article
Biochemistry & Molecular Biology
Zhi-Min Li, Ziwei Hu, Xiaoqin Wang, Suhang Chen, Weiyan Yu, Jianping Liu, Zhimin Li
Summary: In this study, the biochemical and structural properties of two α-ketoglutarate decarboxylases from cyanobacteria were investigated. It was found that the enzyme encoded by the MAE_06010 gene exhibited higher catalytic efficiency compared to the one encoded by the MiAbw_01735 gene. Further investigation revealed that MaKGD showed optimum activity at pH 6.5-7.0 and was most activated by Mg2+. The structural modeling and mutagenesis study provided insights into the binding pocket of MaKGD and the importance of certain residues in its catalysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Leiye Yu, Licong He, Bing Gan, Rujuan Ti, Qingjie Xiao, Hongli Hu, Lizhe Zhu, Sheng Wang, Ruobing Ren
Summary: This study reveals the activation and regulation mechanism of human S1PR1 receptor through cryo-electron microscopy analysis. The S1PR1 receptor plays an essential role in the immune and vascular systems, and understanding its function in depth contributes to the development of related therapeutic approaches.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agriculture, Multidisciplinary
Katarzyna Szambelan, Artur Szwengiel, Jacek Nowak, Jakub Frankowski, Henryk Jelen
Summary: The present study demonstrates the superiority of bioethanol production from sorghum using the granular starch degrading enzyme Stargen (TM) 002 compared to simultaneous saccharification and fermentation and separate hydrolysis and fermentation. Bacteria produce ethanol at a higher yield than yeast in all fermentations. Using Z. mobilis for simultaneous saccharification and fermentation and Stargen (TM) 002 for fermentation resulted in the highest ethanol yield.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Genetics & Heredity
Jin-Cheng Rong, Bo-Wen Ji, Ning Zheng, Zhong-Zhi Sun, Yi-Song Li, Bin-Bin Xie
Summary: Pyruvatibacter mobilis is a marine bacterium that requires pyruvate, encoding antioxidant genes to relieve cellular oxidative stress. The addition of pyruvate to the medium may reduce extracellular ROS, providing insights into the antioxidant activities of bacteria surviving in oxygen-enriched habitats.
Article
Biotechnology & Applied Microbiology
Nosaibeh Nosrati-Ghods, Susan T. L. Harrison, Adeniyi J. Isafiade, Siew Leng Tai
Summary: This study investigated the relationship between wild type and engineered strains of the same microorganism in terms of kinetic parameters, and proposed a set of correction factors to estimate kinetic constant values for species or strains for which they are not available. The results showed a correlation between wild type and engineered strains, with specific substrate limitation constants being higher in the engineered strains. These correction factors were found to be conserved and could serve as a guide for estimating kinetic constants in other species or strains.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Food Science & Technology
S. H. Patel, J. P. Tan, R. A. Borner, S. J. Zhang, S. Priour, A. Lima, C. Ngom-Bru, P. D. Cotter, S. Duboux
Summary: Through metagenomics, chemical profiling, and sensory evaluation, the dynamic nature of water kefir fermentation and the contribution of different microbial members to flavor have been elucidated. This research not only enhances our understanding of water kefir microbiology, but also provides a foundation for the development of defined starter cultures that can reproduce the main attributes of traditionally brewed water kefir.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2022)
Article
Green & Sustainable Science & Technology
Katarzyna Szambelan, Artur Szwengiel, Jacek Nowak, Henryk Jelen, Jakub Frankowski
Summary: This study investigated the effects of using Zymomonas mobilis and Saccharomyces cerevisiae for simultaneous saccharification and fermentation of sorghum grain, and replacing half the water with a stillage liquid fraction on ethanol yield and volatile compound synthesis. The results showed that Z. mobilis is more efficient in ethanol production with a shorter fermentation time and lower fermentation temperature. Reusing the liquid fraction of the stillage did not increase the amount of volatile compounds, and there were significant differences in the volatile compounds profile of bacterial and yeast raw distillates.
JOURNAL OF CLEANER PRODUCTION
(2022)
Review
Biochemistry & Molecular Biology
Hong-Yan Lin, Jin Dong, Jiangqing Dong, Wen-Chao Yang, Guang-Fu Yang
Summary: 4-Hydroxyphenylpyruvate dioxygenase (HPPD) is a crucial enzyme in tyrosine metabolism and a potential target for herbicide and drug discovery. This study summarizes the structures of HPPD complexed with various molecules and analyzes the inhibitory mechanisms of different inhibitors. By identifying key structural factors, the study provides insights into the design of new inhibitors with enhanced potency and selectivity.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Anchittha Kaewchana, Atiya Techaparin, Nongluck Boonchot, Pornthap Thanonkeo, Preekamol Klanrit
Summary: This study developed a Z. mobilis R301 strain overexpressing groESL genes for high-temperature ethanol production from sweet sorghum juice, demonstrating improved ethanol yield and growth performance under various stresses compared to the wild-type strain. The overexpression of groESL led to an increased ethanol production at 40 degrees C, with a higher theoretical ethanol yield and productivity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Energy & Fuels
Ruplappara Sharath Kumar, Pritam Singh, Sanjoy Ghosh
Summary: A two-stage sequential fermentation was conducted to convert mixed sugars into bioethanol in a single bioreactor vessel, with glucose fermentation at stage-1 and xylose fermentation at stage-2. The removal of residual ethanol and Z. mobilis cells in stage-2 led to improved ethanol productivity and overall ethanol titer.
Article
Chemistry, Multidisciplinary
Andre A. Neves, Yelena A. Wainman, Alan Wright, Mikko I. Kettunen, Tiago B. Rodrigues, Sarah McGuire, De-En Hu, Flaviu Bulat, Simonetta Geninatti Crich, Henning Stoeckmann, Finian J. Leeper, Kevin M. Brindle
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2016)
Article
Microbiology
Miguel A. Matilla, Finian J. Leeper, George P. C. Salmond
ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Chemistry, Organic
T. Masini, B. Lacy, L. Monjas, D. Hawksley, A. R. de Voogd, B. Illarionov, A. Iqbal, F. J. Leeper, M. Fischer, M. Kontoyianni, A. K. H. Hirsch
ORGANIC & BIOMOLECULAR CHEMISTRY
(2015)
Article
Chemistry, Multidisciplinary
William J. K. Crone, Natalia M. Vior, Javier Santos-Aberturas, Lukas G. Schmitz, Finian J. Leeper, Andrew W. Truman
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2016)
Article
Chemistry, Physical
S. A. Goussous, M. T. L. Casford, A. C. Murphy, G. P. C. Salmond, F. J. Leeper, P. B. Davies
JOURNAL OF PHYSICAL CHEMISTRY B
(2017)
Article
Biotechnology & Applied Microbiology
Elena Kasparyan, Michael Richter, Carola Dresen, Lydia S. Walter, Georg Fuchs, Finian J. Leeper, Tobias Wacker, Susana L. A. Andrade, Geraldine Kolter, Martina Pohl, Michael Mueller
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2014)
Article
Chemistry, Organic
Amjid Iqbal, El-Habib Sahraoui, Finian J. Leeper
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2014)
Article
Biochemical Research Methods
Andre A. Neves, Henning Stoeckmann, Yelena A. Wainman, Joe C-H. Kuo, Sarah Fawcett, Finian J. Leeper, Kevin M. Brindle
BIOCONJUGATE CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Shaun Stairs, Andre A. Neves, Henning Stoeckmann, Yelena A. Wainman, Heather Ireland-Zecchini, Kevin M. Brindle, Finian J. Leeper
Article
Biochemistry & Molecular Biology
Junhong Guo, Fanglu Huang, Chuan Huang, Xiaobo Duan, Xinyun Jian, Finian Leeper, Zixin Deng, Peter F. Leadlay, Yuhui Sun
CHEMISTRY & BIOLOGY
(2014)
Article
Chemistry, Multidisciplinary
Stefan Luedtke, Piotr Neumann, Karl M. Erixon, Finian Leeper, Ronald Kluger, Ralf Ficner, Kai Tittmann
Article
Chemistry, Organic
Maryam Beigi, Sabrina Loschonsky, Patrizia Lehwald, Volker Brecht, Susana L. A. Andrade, Finian J. Leeper, Werner Hummel, Michael Mueller
ORGANIC & BIOMOLECULAR CHEMISTRY
(2013)
Article
Multidisciplinary Sciences
Cheryl A. Kreinbring, Stephen P. Remillard, Paul Hubbard, Heather R. Brodkin, Finian J. Leeper, Dan Hawksley, Elaine Y. Lai, Chandler Fulton, Gregory A. Petsko, Dagmar Ringe
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Chemistry, Multidisciplinary
Laia Josa-Cullere, Yelena A. Wainman, Kevin M. Brindle, Finian J. Leeper
Article
Oncology
Flaviu Bulat, Friederike Hesse, Bala Attili, Chandra Solanki, Iosif A. Mendichovszky, Franklin Aigbirhio, Finian J. Leeper, Kevin M. Brindle, Andre A. Neves
Summary: There is a clinical demand for imaging agents that can detect tumor cell death after treatment. We present a gallium-68-labeled derivative of Synaptotagmin-I for imaging tumor cell death using PET. Ga-68-C2Am could be a valuable tool for rapidly assessing tumor responses to treatment.