Article
Chemistry, Medicinal
Zhenhao Liu, Kuan Liang, Wenlang Liu, Tao Jiang, Rui Zhou, Mojie Duan, Zheng Zheng
Summary: The tyrosine-protein kinase Met (c-Met) is a crucial signaling molecule involved in cellular growth and division. Dysregulation of c-Met can lead to various fatal diseases. Understanding the mechanisms of ligand binding and regulation of c-Met is important for developing effective drugs against drug tolerance.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Oncology
Shuangqing Liu, Huilei Zhang, Yanan Li, Yana Zhang, Yangyang Bian, Yanqiong Zeng, Xiaohan Yao, Jiajia Wan, Xu Chen, Jianru Li, Zhaoqing Wang, Zhihai Qin
Summary: S100A4 plays a crucial role in promoting the protumor polarization of TAMs by upregulating fatty acid oxidation through the PPAR-gamma pathway. IL-4 induction leads to an increase in S100A4 expression, which in turn controls PPAR-gamma upregulation and enhances fatty acid absorption and oxidation in TAMs. Targeting macrophage-derived S100A4 may be a potential strategy to prevent TAMs from acquiring a protumor phenotype.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Chemistry, Multidisciplinary
Ming Zhang, Zhenhao Tian, Jiayue Wang, Xiangge Tian, Chao Wang, Jingnan Cui, Xiaokui Huo, Lei Feng, Zhenlong Yu, Xiaochi Ma
Summary: Leucine aminopeptidase (LAP) is a key hydrolytic enzyme for specific peptides and proteins, and is known as a crucial virulence factor for pathogenic infections. The development of a new fluorescent probe DDBL allows real-time detection of LAP expression in bacteria from feces, and is also used for high-throughput screening to identify inhibitors.
Article
Multidisciplinary Sciences
Kathryn M. Pflug, Dong W. Lee, Kassandra McFadden, Linda Herrera, Raquel Sitcheran
Summary: The prognosis of high-grade gliomas, such as glioblastoma multiforme (GBM), is very poor due to their highly invasive nature. Previous studies have shown that TWEAK can promote the invasiveness of GBM cells through the noncanonical NF-.B pathway, with NIK being a key player. Our study reveals that NIK is upregulated at the transcriptional level in invading cells, and high expression of NIK facilitates collective invasion of GBM cells. Additionally, we demonstrate that E2F4 and E2F5 transcription factors are involved in the regulation of NIK transcription and the promotion of GBM cell invasion in response to TWEAK.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Keizo Takenaga, Takahiro Ochiya, Hideya Endo
Summary: The NBD peptide inhibits invasiveness and metastasis of highly metastatic human mammary carcinoma cells by suppressing MMP-14 expression through Sp1 downregulation. The peptide also has potential as a promising cancer therapeutic drug for targeting both endothelial cells and tumor cells.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Megan L. Ken, Rohit Roy, Ainan Geng, Laura R. Ganser, Akanksha Manghrani, Bryan R. Cullen, Ursula Schulze-Gahmen, Daniel Herschlag, Hashim M. Al-Hashimi
Summary: Cellular processes are the result of interactions between biomolecules, which form biologically active complexes. These interactions are mediated by intermolecular contacts, and changes in biomolecule conformations are required for the formation of these contacts. Binding affinity and cellular activity depend on both the strength of the contacts and the propensities to form binding-competent conformational states. However, limitations have hindered the study of how conformational propensities affect cellular activity. This study focuses on the propensities of HIV-1 TAR RNA and how they predict binding affinities and cellular activity.
Article
Biophysics
Caroline G. Sanz, Anca Aldea, Daniela Oprea, Melania Onea, Adrian T. Enache, Madalina M. Barsan
Summary: A novel electrochemical biosensor was developed for the first time to monitor fibroblast cells stress levels in situ under external stimuli. The biosensor exhibited good detection performance in cell culture media, with high sensitivity and correlation. The results suggest the potential application of this biosensor in electrochemical screening of fibroblast cell stress.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Medicinal
Tao Jiang, Zhenhao Liu, Wenlang Liu, Jiawen Chen, Zheng Zheng, Mojie Duan
Summary: In this study, using metadynamics simulation, the conformational transition pathways of the DFG flipping for the c-Met kinase were analyzed. Two distinct pathways, DFG-up and DFG-down, were identified and their free energy profiles were calculated. The findings provide insights into the conformation-activity relationship for c-Met and may suggest a new drug target.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Biochemistry & Molecular Biology
Li Chengling, Zhang Yulin, Xie Xiaoyu, Lu Xingchen, Zhang Sen, Wang Ziming, Chen Xianming
Summary: miR-325-3p inhibits osteoclast formation by targeting S100A4 in osteoclast precursors, leading to decreased osteoclast number and attenuated bone resorption in colorectal cancer bone metastasis.
MOLECULAR MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Junxing Zhao, Zhichao Tang, Manikandan Selvaraju, Kristen A. Johnson, Justin T. Douglas, Philip F. Gao, H. Michael Petrassi, Michael Zhuo Wang, Jingxin Wang
Summary: In this study, a novel platform for target identification of activators of signaling pathways using a CRISPR knockout library was reported. This platform enables the uncovering of drug targets and essential genes required for the activity of small molecules through sequencing.
ACS CENTRAL SCIENCE
(2022)
Article
Biophysics
Kun Liu, Yunsen Zhang, Ke Liu, Yunqiu Zhao, Bei Gao, Xinyi Tao, Ming Zhao, Feng-Qing Wang, Dongzhi Wei
Summary: In this study, a de novo designed transcription factor (TF) named DLA was successfully developed for the construction of a biosensor for progesterone detection. The biosensor showed high sensitivity, a wide detection range, and rapid detection time. Furthermore, the strategy used in this study could be applied to develop various other biosensors.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Cell Biology
Christoph Treese, Kimberly Hartl, Michelle Potzsch, Matthias Dahlmann, Moritz von Winterfeld, Erika Berg, Michael Hummel, Lena Timm, Beate Rau, Wolfgang Walther, Severin Daum, Dennis Kobelt, Ulrike Stein
Summary: S100A4 plays a key role in the metastasis of adenocarcinoma of the gastroesophageal junction and stomach, and its high expression is significantly correlated with poor prognosis and metastasis development.
Article
Cell Biology
Jeongmin Ju, Hae Nim Lee, Lin Ning, Hyunjoo Ryu, Xin X. Zhou, Hyeyeon Chun, Yong Woo Lee, Austin I. Lee-Richerson, Cherlhyun Jeong, Michael Z. Lin, Jihye Seong
Summary: The study investigates how RhoA activity affects focal adhesion, revealing that submaximal RhoA activation leads to selective focal adhesion disassembly and identifies Src as an amplitude-dependent effector of RhoA. Pharmacological inhibition of Src reverses the direction of focal adhesion response to RhoA activation from disassembly to growth, demonstrating Src's role in suppressing focal adhesion growth upon RhoA activation.
Review
Mathematical & Computational Biology
Nikolay Dokholyan
Summary: The advancement in protein design has led to the potential development of a nanoscale programming language, utilizing molecules as operands and their conformational states as logic gates. By harnessing natural evolutionary processes, code can evolve during computation, leading to revolutionary algorithmic developments with wide-ranging impacts on biotechnological applications. NCAs based on single-chain proteins have the ability to revolutionize studies of biological systems and enable deeper understanding of human biology and disease.
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2021)
Article
Cell Biology
Amanda E. Balaban, Ly T. S. Nguyen, Eleana Parajon, Douglas N. Robinson
Summary: Nonmuscle myosin IIB (NMIIB) is a primary force generator during cell motility. Adding back NMIIB and strategic mutants have specific consequences for cell morphology, metabolism, cortical tension, mechanoresponsiveness, and gene expression. Major modes of ATP production and metabolic and growth pathways undergo significant changes in gene expression.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Cell Biology
Benjamin P. Bouchet, Ivar Noordstra, Miranda van Amersfoort, Eugene A. Katrukha, York-Christoph Ammon, Natalie D. ter Hoeve, Louis Hodgson, Marileen Dogterom, Patrick W. B. Derksen, Anna Akhmanova
DEVELOPMENTAL CELL
(2016)
Article
Immunology
Veronika Miskolci, Bin Wu, Yasmin Moshfegh, Dianne Cox, Louis Hodgson
JOURNAL OF IMMUNOLOGY
(2016)
Article
Cell Biology
Sara K. Donnelly, Ramon Cabrera, Serena P. H. Mao, John R. Christin, Bin Wu, Wenjun Guo, Jose Javier Bravo-Cordero, John S. Condeelis, Jeffrey E. Segall, Louis Hodgson
JOURNAL OF CELL BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Jessica Aijia Liu, Yanxia Rao, May Pui Lai Cheung, Man-Ning Hui, Ming-Hoi Wu, Lo-Kong Chan, Irene Oi-Lin Ng, Ben Niu, Kathryn S. E. Cheah, Rakesh Sharma, Louis Hodgson, Martin Cheung
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Samer J. Hanna, Kessler McCoy-Simandle, Veronika Miskolci, Peng Guo, Michael Cammer, Louis Hodgson, Dianne Cox
SCIENTIFIC REPORTS
(2017)
Correction
Biochemistry & Molecular Biology
Daria M. Shcherbakova, Natasha Cox Cammer, Tsipora M. Huisman, Vladislav V. Verkhusha, Louis Hodgson
NATURE CHEMICAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Daria M. Shcherbakova, Natasha Cox Cammer, Tsipora M. Huisman, Vladislav V. Verkhusha, Louis Hodgson
NATURE CHEMICAL BIOLOGY
(2018)
Article
Cell Biology
Nicholas D. Condon, John M. Heddleston, Teng-Leong Chew, Lin Luo, Peter S. McPherson, Maria S. Ioannou, Louis Hodgson, Jennifer L. Stow, Adam A. Wall
JOURNAL OF CELL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Jeanine Pignatelli, Jose Javier Bravo-Cordero, Minna Roh-Johnson, Saumil J. Gandhi, Yarong Wang, Xiaoming Chen, Robert J. Eddy, Alice Xue, Robert H. Singer, Louis Hodgson, Maja H. Oktay, John S. Condeelis
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Katrina M. Wisdom, Kolade Adebowale, Julie Chang, Joanna Y. Lee, Sungmin Nam, Rajiv Desai, Ninna Struck Rossen, Marjan Rafat, Robert B. West, Louis Hodgson, Ovijit Chaudhuri
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Taras A. Redchuk, Maksim M. Karasev, Polina V. Verkhusha, Sara K. Donnelly, Maren Huelsemann, Jori Virtanen, Henna M. Moore, Maria K. Vartiainen, Louis Hodgson, Vladislav V. Verkhusha
NATURE COMMUNICATIONS
(2020)
Article
Cell Biology
Ryan Baumert, Hong Ji, Adriana Paulucci-Holthauzen, Aaron Wolfe, Cari Sagum, Louis Hodgson, Jyothi Arikkath, Xiaojiang Chen, Mark T. Bedford, M. Neal Waxham, Pierre D. McCrea
JOURNAL OF CELL BIOLOGY
(2020)
Article
Biology
Maren Hulsemann, Colline Sanchez, Polina V. Verkhusha, Vera Des Marais, Serena P. H. Mao, Sara K. Donnelly, Jeffrey E. Segall, Louis Hodgson
Summary: The study reveals that TC10 GTPase regulates MT1-MMP at invadopodia during breast cancer metastasis, with its activity and function modulated by p190RhoGAP and Exo70.
COMMUNICATIONS BIOLOGY
(2021)
Article
Oncology
Brian C. H. Cheung, Louis Hodgson, Jeffrey E. Segall, Mingming Wu
Summary: One of the hallmarks of cancer cells is their exceptional ability to migrate within the extracellular matrix (ECM) for gaining access to the circulatory system. RhoA plays a key role in cell migration and its activity differs in 2D and 3D environments. Cell behavior and RhoA polarization are also moderately correlated with cell shape.
EXPERIMENTAL CELL RESEARCH
(2022)
Correction
Biology
Maren Hulsemann, Colline Sanchez, Polina V. Verkhusha, Vera Des Marais, Serena P. H. Mao, Sara K. Donnelly, Jeffrey E. Segall, Louis Hodgson
COMMUNICATIONS BIOLOGY
(2021)