Article
Biochemistry & Molecular Biology
Elisabeth Darrouzet, Clara Rinaldi, Barbara Zambelli, Stefano Ciurli, Christine Cavazza
Summary: Nickel insertion into nickel-dependent CODH is a key step in enzyme activation, with CooJ playing a significant role in this process by binding Ni(II) ions through HAS motif. CooJ homologues exhibit distinct motifs for Ni(II) binding, suggesting potential significance in CODH maturation and nickel homeostasis.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Genetics & Heredity
Yan Zhao, Wei Xu, Lijing Wang, Shuai Han, Yongzhong Zhang, Qingzhi Liu, Baoshen Liu, Xiangyu Zhao
Summary: In this study, we identified a light and temperature-dependent mutant (nec-t) in maize with necrotic spots and yellow-green leaves. Through map-based cloning and allelic tests, we found that nec-t is a novel allelic mutant of the Necrotic4 gene. The mutation in Necrotic4 resulted in enhanced chlorophyll degradation and reactive oxygen species response. Our research provides insights into the molecular mechanism of necrotic initiation in maize.
Article
Multidisciplinary Sciences
Yuan Tian, Ishara Datta, Rui Yang, Chun Wan, Bing Wang, Lauren Crisman, Huan He, Chad A. Brautigam, Suzhao Li, Jingshi Shen, Qian Yin
Summary: We found that AAGAB exists as a dimer before binding to AP1/2, and its dimerization is mediated by the C-terminal domain. AAGAB uses the same C-terminal domain to recognize and stabilize the subunits in the AP1 and AP2 complexes, forming binary complexes with only one copy of AAGAB.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Pharmacology & Pharmacy
Meredith R. McGuire, Peter J. Espenshade
Summary: Progesterone Receptor Membrane Component 1 (PGRMC1) is a heme-binding protein involved in various cellular and tissue functions. However, the underlying mechanisms of PGRMC1 in these biological aspects remain largely unknown. This review provides an overview of the literature on PGRMC1, highlighting supported information and areas for further investigation. The central role of PGRMC1 in cytochrome P450 activity is extensively discussed, and a speculative model is proposed regarding its function as a heme chaperone. By identifying knowledge gaps, this review aims to inspire a better understanding of this enigmatic protein.
PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Biology
Alonso Javier Pardal, Andrew James Bowman
Summary: This study identified importin-5 as the major importin associated with cytoplasmic H3, which transfers its monomeric cargo to nuclear sNASP. Additionally, monomeric H4 interacts specifically with HAT1 and RBBP7. It was found that Imp5 and sNASP compete for binding H3, suggesting a direct interaction site competition requiring GTP-bound Ran for histone transfer.
Article
Chemistry, Medicinal
Alexandra Glenister, Catherine K. J. Chen, David J. Paterson, Anna K. Renfrew, Michela Simone, Trevor W. Hambley
Summary: A glucose-based vector conjugated to a tris(methylpyridyl)amine ligand is described for generating targeted chaperone and caging complexes for active anticancer agents. The ligand inhibits hexokinase in cells, showing potential for phosphorylation and targeting cancer cells. The Co(III) complex incorporating this ligand demonstrates glucose-dependent cellular accumulation and potential for targeted delivery of highly toxic anticancer agents.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Microbiology
Sirui Han, Kailun Guo, Wei Wang, Yizhi J. Tao, Haichun Gao
Summary: In this study, HtpA was identified as a heme-trafficking protein in the bacterium Shewanella oneidensis. The absence of HtpA significantly reduced cytochrome c content and catalase activity. This research sheds light on the mechanism of heme transport across membranes in bacteria and extends the understanding of TANGO2 proteins.
Article
Biochemistry & Molecular Biology
Narayanan Srividya, Iris Lange, Jana K. Richter, Matthias Wuest, B. Markus Lange
Summary: In this study, the enantioselectivity of multiple enzymes involved in monoterpenoid biosynthesis in peppermint and Japanese catnip was investigated. The experimental results showed that these enzymes exhibited different enantioselectivity towards intermediates of the pathway, and molecular modeling studies supported the experimental data. This study lays the groundwork for further structure-function studies on the origin of enantioselectivity in closely related species of the Lamiaceae.
Article
Biochemistry & Molecular Biology
Zak Vilen, Eugene Joeh, Elizabeth Lee, Mia L. Huang
Summary: The surface proteome plays a crucial role in cellular biology, enabling cell-to-cell interactions and communication with extracellular biomolecules. Understanding the composition of the surfaceome can facilitate the identification of biomarkers and pharmacological targets. This study utilized a surfaceome proximity labeling method combined with quantitative mass spectrometry-based proteomics to investigate the changes in the surfaceome of hepatic stellate cells in response to the loss of Basigin. The results demonstrated that Basigin loss leads to reductions in the cell surface expression of monocarboxylate transporters MCT1 and MCT4, highlighting the utility of the surfaceome proximity labeling method in identifying cell surface chaperone protein clients.
Article
Biochemistry & Molecular Biology
Yue Dai, Angela S. Fleischhacker, Liu Liu, Sara Fayad, Amanda L. Gunawan, Dennis J. Stuehr, Stephen W. Ragsdale
Summary: GAPDH is involved in heme delivery to HO2, maintaining cellular heme homeostasis.
BIOLOGICAL CHEMISTRY
(2022)
Article
Microbiology
Keke Fei, Huan Yan, Xiaoyan Zeng, Shaojia Huang, Wei Tang, Matthew S. Francis, Shiyun Chen, Yangbo Hu
Summary: This study reveals a negative regulatory loop in which LcrQ cooperates with the YopD-LcrH complex to inhibit krF expression at the posttranscriptional level. This loop involves the mRNA decay factor RNase E and its associated RhIB protein, recruited to the regulatory complex by LcrQ and LcrH. The findings shed new light on a mechanism inhibiting T3SS production and biogenesis.
Article
Biochemistry & Molecular Biology
Anastasya Boyarski, Noam Shlush, Shiraz Paz, Jerry Eichler, Lital Alfonta
Summary: In this study, four different enzymatic constructs of L-lactate dehydrogenase were compared and the fusion enzyme containing two cytochromes showed superior catalytic and electrochemical properties. This fusion enzyme exhibited higher activity, lower redox potential, wider dynamic range, and higher product inhibition concentration than the native enzyme. Additionally, it displayed measurable lactate-dependent oxygen reduction.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Roberta Sgammato, Niels Van Brempt, Roy Aerts, Sabine Van Doorslaer, Sylvia Dewilde, Wouter Herrebout, Christian Johannessen
Summary: Protoglobin from Methanosarcina acetivorans (MaPgb) is a dimeric globin belonging to the same lineage as globin-coupled sensors. It has a putative role in scavenging reactive nitrogen and oxygen species. Through various spectroscopic techniques, the unusual reaction of ferric MaPgb with nitrite was revealed. Unlike other globins, nitrite did not induce the formation of a nitriglobin form in MaPgb. Instead, it led to the formation of a stable nitric-oxide ligated ferric form of the protein (MaPgb-NO) in mildly acidic pH. The changes in the optical and vibrational properties of MaPgb upon nitric-oxide ligation provide insights into its gas-binding mechanism.
DALTON TRANSACTIONS
(2023)
Article
Multidisciplinary Sciences
Agnes Moe, Justin Di Trani, John L. Rubinstein, Peter Brzezinski
Summary: Energy conversion in aerobic organisms involves electron transfer from low-potential donors to dioxygen through the membrane-bound respiratory chain. The formation of supercomplex CIII2CIV1/2 facilitates electron transfer by two-dimensional diffusion of cyt. c, increasing QH(2):O-2 oxidoreductase activity and suggesting a regulatory role for supercomplex formation in the respiratory chain.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Jiao Li, Wan Zheng, Ming Gu, Long Han, Yanmei Luo, Koukou Yu, Mengxin Sun, Yuliang Zong, Xiuxiu Ma, Bing Liu, Ethan P. Lowder, Deanna L. Mendez, Robert G. Kranz, Kai Zhang, Jiapeng Zhu
Summary: This study presents high resolution cryo-EM structures of CcmABCD in different states and proposes a hypothetical model of heme trafficking, transfer, and release of holoCcmE.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Elisabeth Fischermeier, Petr Pospisil, Ahmed Sayed, Martin Hof, Marc Solioz, Karim Fahmy
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)
Article
Biochemistry & Molecular Biology
Vera Haskamp, Simone Karrie, Toni Mingers, Stefan Barthels, Francois Alberge, Axel Magalon, Katrin Mueller, Eckhard Bill, Wolfgang Lubitz, Kirstin Kleeberg, Peter Schweyen, Martin Broering, Martina Jahn, Dieter Jahn
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Microbiology
Stefano Mancini, Ranjeet Kumar, Veena Mishra, Marc Solioz
Article
Biochemistry & Molecular Biology
Denitsa Eckweiler, Christian-Alexander Dudek, Juliane Hartlich, David Broetje, Dieter Jahn
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Maike K. Groenewold, Marco Massmig, Stefanie Hebecker, Linna Danne, Zofia Magnowska, Manfred Nimtz, Franz Narberhaus, Dieter Jahn, Dirk W. Heinz, Lothar Jaensch, Juergen Moser
BIOCHEMICAL JOURNAL
(2018)
Article
Multidisciplinary Sciences
Evgeny Petrov, Gopalakrishnan Menon, Paul R. Rohde, Andrew R. Battle, Boris Martinac, Marc Solioz
Article
Microbiology
Christian Kirchhoff, Matthias Ebert, Dieter Jahn, Heribert Cypionka
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Elisabeth Haertig, Claudia Fraedrich, Maren Behringer, Anja Hartmann, Meina Neumann-Schaal, Dieter Jahn
MOLECULAR MICROBIOLOGY
(2018)
Article
Microbiology
Julia D. Hofmann, Andreas Otto, Mareike Berges, Rebekka Biedendieck, Annika-Marisa Michel, Doerte Becher, Dieter Jahn, Meina Neumann-Schaal
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Maike K. Groenewold, Stefanie Hebecker, Christiane Fritz, Simon Czolkoss, Milan Wiesselmann, Dirk W. Heinz, Dieter Jahn, Franz Narberhaus, Meriyem Aktas, Juergen Moser
MOLECULAR MICROBIOLOGY
(2019)
Article
Microbiology
Isabel Bernal, Julia Danielle Hofmann, Bjoern Bulitta, Frank Klawonn, Annika-Marisa Michel, Dieter Jahn, Meina Neumann-Schaal, Dunja Bruder, Lothar Jaensch
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Jan Jasper, Jose Ramos, Christian Trncik, Dieter Jahn, Oliver Einsle, Gunhild Layer, Juergen Moser
Editorial Material
Medicine, Research & Experimental
Marc Solioz
EXPERIMENTAL BIOLOGY AND MEDICINE
(2020)
Article
Microbiology
Ilse M. Boekhoud, Annika-Marisa Michel, Jeroen Carver, Dieter Jahn, Wiep Klaas Smits
Review
Biochemical Research Methods
Wei He, Alexander Heinz, Dieter Jahn, Karsten Hiller
Summary: Macrophages play a crucial role in combating infection and supporting tissue recovery post pathogen clearance, requiring metabolic reprogramming to provide sufficient energy and metabolites. Recent studies have shown that certain metabolic intermediates can directly control macrophage activation and effector functions, with citrate, succinate, and itaconate playing key roles in modulating immune responses.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)