Article
Nanoscience & Nanotechnology
Xing Xing, Yao Zhou, Yelu Wei, Yuchen Zhang, Zaiqin Man, Weihua Zhang, Zhenda Lu
Summary: We developed a robust method using reverse micelles to print molecules/ions into arbitrarily defined patterns with sub-20 nm precision. The reverse micelles serve as nano-sized vessels that can load molecules/ions and be patterned onto predefined positions by electrostatic attraction. This method allows for flexible adjustment of the number of molecules/ions, spot spacing, and pattern shapes, achieving 10 nm position accuracy, 30 nm spot size, and 100 nm spot spacing (>250,000 DPI). Water-soluble dye molecules, protein molecules, and chloroaurate ions were successfully patterned into nanoarrays, providing a platform for the convenient, flexible, and robust fabrication of functional molecule/ion-based nanodevices, such as biochips, for high-throughput and ultrasensitive analysis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Multidisciplinary Sciences
Kun Ma, Shuai Xu, Tongxiang Tao, Junchao Qian, Qiqi Cui, Sajid Ur Rehman, Xiaoguang Zhu, Ruiguo Chen, Hongxin Zhao, Changhao Wang, Ziping Qi, Han Dai, Xin Zhang, Can Xie, Yang Lu, Hongzhi Wang, Junfeng Wang
Summary: A magnetosome-like nanoreactor was constructed in this study, showing great potential for magnetic enrichment in a tumor mouse model with good monodispersion and smaller particle size.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Alyssa B. Sanders, Jacob T. Zangaro, Nakoa K. Webber, Ryan P. Calhoun, Elizabeth A. Richards, Samuel L. Ricci, Hannah M. Work, Daniel D. Yang, Kaitlyn R. Casey, Joseph C. Iovine, Gabriela Baker, Taylor V. Douglas, Sierra B. Dutko, Thomas J. Fasano, Sarah A. Lofland, Ashley A. Rajan, Mihaela A. Vasile, Benjamin R. Carone, Nathaniel V. Nucci
Summary: Despite advances in small molecule drug encapsulation, encapsulating proteins remains problematic. This study investigates a reverse micelle system as a platform for storage and delivery of biological drugs, and evaluates the effects of different solvents on encapsulation efficiency. The findings provide insights for the future development of long-term room-temperature storage of biologics or water-in-oil-in-water biologic delivery systems.
Review
Pharmacology & Pharmacy
Almudena Naharros-Molinero, Maria Angela Caballo-Gonzalez, Francisco Javier de la Mata, Sandra Garcia-Gallego
Summary: This review provides an overview of the main parameters affecting the micellization process of Pluronics and presents common techniques for characterizing the structure and properties of the micelles. It also discusses approaches to improving the stability and drug loading of Pluronic micelles, with a focus on reverse Pluronics and reverse micelles which have been underexplored in the literature.
Article
Chemistry, Applied
Baochen Fang, Zixuan Gu, Jae-Bom Ohm, Bingcan Chen, Jiajia Rao
Summary: This study investigated the influence of the defatting process on the extraction performance and properties of hemp protein isolate (HPI) obtained via reverse micelles (RMs) extraction. The results showed that defatting is not necessary for protein extraction and HPIs obtained from non-defatted hemp flour and defatted hemp flour exhibited similar composition, functionalities, and protein subunits. However, HPI from non-defatted hemp flour exhibited stronger gel properties and higher content of aromatic compounds.
FOOD HYDROCOLLOIDS
(2023)
Article
Nanoscience & Nanotechnology
Ankan Kumar Sarkar, Koushik Debnath, Himali Arora, Pankaj Seth, Nihar R. Jana, Nikhil R. Jana
Summary: The direct cytosolic delivery of large biomolecules bypassing endocytic pathways is a promising strategy for therapeutic applications. Recent research has shown that surface chemistry of carriers, nanoscale assembly, colloidal stability, and low surface charge are critical for non-endocytic uptake processes. The guanidinium-terminated polyaspartic acid micelle can deliver protein/DNA directly to the cytosol by forming a nano-assembly with <200 nm size and near-zero surface charge, highlighting the importance of size and colloidal properties for efficient cytosolic delivery of large biomolecules.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Inorganic & Nuclear
Lubing Qin, Feng Zhang, Xiaoshuang Ma, Yun Tang, Guanyu Ma, Zhenghua Tang
Summary: In order to advance the development of atomically precise Ag and Ag-alloyed nanoclusters, an effective synthetic method using the CTAB reverse micelle approach was successfully extended to quickly and efficiently synthesize high-purity Ag and Ag-alloyed nanoclusters. The study also elucidated the mechanism behind the reverse micelle method through characterization techniques.
DALTON TRANSACTIONS
(2021)
Article
Chemistry, Physical
Anagha Anilkumar, Sharmistha Dutta Choudhury
Summary: Reverse poloxamines are important building blocks that can be assembled into useful biocompatible microstructures by saccharide excipients. Glucose and sucrose have a positive effect on the micellization of reverse poloxamines, with sucrose being more favorable. Furthermore, pH conditions can convert the micelles from nonionic to cationic, opening new opportunities for controlled assembly and clustering of reverse poloxamines.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Engineering, Environmental
Jiajing Chen, Mengchen Xu, Yongxin Zhang, Linlin Xu, Shuyue Zhao, Yingli An, Rujiang Ma, Yang Liu, Feihe Ma, Linqi Shi
Summary: A novel allosteric synthetic antibody (Allo-SyAb) was constructed using mixed-shell polymeric micelle, which can spatiotemporally activate in response to tumor microenvironment for cancer immunotherapy.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Ziyao Chang, Wenhao Wang, Zhengwei Huang, Ying Huang, Chuanbin Wu, Xin Pan
Summary: Protein drugs in pressurized metered dose inhalers (pMDIs) have potential for research and applications, but the stability of carrier particles is a major obstacle. This study proposes using lecithin to construct a reverse micelle system, aiming to improve the stability of carrier particles in pMDIs and protect protein drugs from degradation.
ADVANCED THERAPEUTICS
(2023)
Article
Spectroscopy
Shiying Wang, Mian Wang, Li Han, Yan Sun, Wensheng Cai, Xueguang Shao
Summary: This study investigates the stability of proteins in a confined environment using near-infrared (NIR) spectra. It is found that proteins undergo denaturation in aqueous solutions but not in reverse micelles (RMs). Changes in the spectral bands of water provide evidence for protein denaturation and suggest that water can serve as a probe for structural changes in proteins. The presence of bridging water between the protein and inner surface of RMs may be the reason for stability.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Applied
Lifen Zhang, Mingzhu Zhang, Xiaoyang Sun, Fusheng Chen, Qian Wu
Summary: The study investigated the extraction efficiency, structure, and emulsifying properties of soybean proteins obtained through AOT reverse micelle, showing significant changes in structure and emulsifying properties. The results indicated great application potential of reverse micelle extraction in the food industry.
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2021)
Article
Energy & Fuels
Zechen Zhang, William A. Ducker
Summary: This paper presents an experimental study on the displacement of oil by water in a nano-scale glass channel. Three surfactants were examined and the results showed that only AOT could cause oil displacement within 12 hours. This study suggests that surfactants enhance the transport of water through oil, aiding in oil recovery.
Review
Materials Science, Multidisciplinary
Pradip Das, Nikhil R. Jana
Summary: Biocompatible and biodegradable polyaspartamide-derived formulations have shown great potential in diverse biomedical applications, with successful utilization as coating materials for inorganic nanoparticles, delivery carriers for therapeutic agents, and scaffolds in tissue engineering. Their simple synthesis methods, biodegradability, and successful preclinical validations contribute to their emerging role in the field of nanomedicine.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Kristen Hong, Minzhi Yu, Julia Crowther, Ling Mei, Karl Olsen, Yonghong Luo, Yuqing Eugene Chen, Yanhong Guo, Anna Schwendeman
Summary: The progression of atherosclerosis is driven by an imbalance of cholesterol and unresolved local inflammation in the arteries. Recent clinical studies have used recombinant apolipoprotein A-I (ApoA-I)-based high-density lipoprotein (HDL) nanoparticles to reduce atheroma size and resolve inflammatory response. This study focuses on the preparation of phospholipid-based, ApoA-I-free micelles that structurally and functionally resemble HDL nanoparticles, and investigates the impact of micelle composition on their anti-inflammatory activity and cholesterol regulation.
Article
Chemistry, Multidisciplinary
Kazumitsu Naoe, Tatsuya Ando, Kenta Kawasaki, Masanao Imai
Article
Biochemistry & Molecular Biology
Ayame Nitani, Hiroya Muta, Masayuki Adachi, Masatomo So, Kenji Sasahara, Kazumasa Sakurai, Eri Chatani, Kazumitsu Naoe, Hirotsugu Ogi, Damien Hall, Yuji Goto
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biotechnology & Applied Microbiology
Kazuhito Nagayama, Ryo Katakura, Takashi Hata, Kazurnitsu Naoe, Masanao Imai
BIOCHEMICAL ENGINEERING JOURNAL
(2008)
Article
Chemistry, Physical
K. Naoe, M. Kataoka, M. Kawagoe
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2010)
Article
Chemistry, Physical
Koichiro Yamanaka, Satoru Nishino, Kazumitsu Naoe, Masanao Imai
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2013)
Meeting Abstract
Biotechnology & Applied Microbiology
Nobuhiro Naito, Junji Takeuchi, Kazumitsu Naoe, Mikio Kawagoe, Masanao Imai, Miyuki Funato
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2009)
Article
Engineering, Chemical
Kazumitsu Naoe, Koichiro Yamanaka, Saori Takeuchi, Mikio Kawagoe, Masanao Imai
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
(2012)
Article
Chemistry, Multidisciplinary
K. Naoe, C. Petit, M. P. Pileni
Article
Biochemistry & Molecular Biology
Shoma Tanaka, Hiroaki Okano, Nobuyuki Matsuda, Jun Sawai, Kazumitsu Naoe, Masanao Imai
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2020)
Article
Chemistry, Physical
Kureha Yamakawa, Airi Hirobe, Satoshi Honjo, Koko Higashio, Jun Sawai, Kazumitsu Naoe, Masanao Imai
Summary: In this study, we used liquid marbles (LMs) for aerobic bacteria culture to produce bacterial cellulose (BC). LMs are droplets stabilized by hydrophobic particles that allow vapor and gas transfer. We successfully prepared LMs containing Komagataeibacter xylinus (K. xylinus) by dropping a culture medium solution onto a calcium stearate powder layer and rolling it with calcium stearate microparticles. After static culture, the LM core solution became a hydrogel due to BC production by the cells. The LM-BC gel had higher water holding capacity and a unique structure compared to conventional BC. The LM interface played a critical role in the properties of the BC produced.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Chemistry, Multidisciplinary
Nozomi Higashide, Nobuyuki Matsuda, Kazumitsu Naoe, Masanao Imai
Summary: This paper reports the application of food-grade magnesium stearate microparticles as a stabilizer in the preparation of biocompatible Pickering emulsions. The study found that stable emulsions could be formed by adjusting the concentration of microparticles and using mild agitation methods. The use of CaCl2 was shown to produce the most stable emulsion, and the size of the emulsion was proportional to agitation speed. Compared to traditional surfactant-based emulsion systems, the novel Pickering emulsion systems demonstrated consistent emulsion preparation without the need for artificial surfactants.
Article
Chemistry, Physical
K. Naoe, C. Petit, M. P. Pileni
JOURNAL OF PHYSICAL CHEMISTRY C
(2007)
Article
Biochemical Research Methods
Kazumitsu Naoe, Chihiro Takeuchi, Mikio Kawagoe, Kazuhito Nagayama, Masanao Imai
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2007)
Article
Biophysics
K Naoe, K Noda, M Kawagoe, M Imai
COLLOIDS AND SURFACES B-BIOINTERFACES
(2004)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)