Article
Biochemistry & Molecular Biology
Jianzhong Huang, Xiaoqiu Wu, Zhiyong Gao
Summary: This study reveals a mechanism for the control of L5 protein stability in plant immunity, where a RING-type E3 ligase regulates the stability of L5 protein through the ubiquitin proteasome system, suppressing L5-triggered cell death.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jianzhong Huang, Xiaoqiu Wu, Kaiting Sun, Zhiyong Gao
Summary: This study investigated the activation mechanism of the AT1G12290 NLR protein by transiently expressing a series of NBS-LRR proteins in Nicotiana benthamiana leaves. The YFP-tagged AT1G12290 was found localized on the plasma membrane, with the myristoylation site Gly2 playing a critical role in protein localization and function. The CC domain was identified as sufficient to activate cell death, with the N-terminal 1-100 amino acid fragment being the minimal region for inducing cell death and self-association.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Wanlu Zhang, Qi Yuan, Yiduo Wu, Jing Zhang, Jingtao Nie
Summary: The NBS-LRR (NLR) gene family, particularly the CC-NBS-LRR(CNL) family, plays a critical role in regulating disease defense response in cucumber plants. This study systematically characterized the CNL genes in cucumber plants and investigated their expression patterns, regulation mechanisms, and responsiveness to different stimuli. The findings provide fundamental information for further understanding the functions of the CNL gene family in cucumber plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yan Liu, Dalong Li, Na Yang, Xiaolong Zhu, Kexin Han, Ran Gu, Junyu Bai, Aoxue Wang, Yaowei Zhang
Summary: The study investigated the characteristics, evolutionary relationships, and pathogen defense roles of the CC type NBS-LRR gene family in Chinese cabbage. By analyzing gene expression and differential levels in response to pathogens, insights into the functions and mechanisms of CC-NBS-LRR genes in Chinese cabbage were provided.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Muhammad Tahir ul Qamar, Muhammad Sadaqat, Xi-Tong Zhu, Huan Li, Xing Huang, Kinza Fatima, Mashal M. Almutairi, Ling-Ling Chen
Summary: This study successfully identified the members of the CNL gene family in different mango cultivars through a pangenome-wide analysis. It provides insights into the genetic diversity, evolutionary relationships, and functional characteristics of CNL genes in mango.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Gui Xiao, Wenjuan Wang, Muxing Liu, Ya Li, Jianbin Liu, Marina Franceschetti, Zhaofeng Yi, Xiaoyuan Zhu, Zhengguang Zhang, Guodong Lu, Mark J. Banfield, Jun Wu, Bo Zhou
Summary: The arms race between plants and pathogens has led to the development of sophisticated recognition mechanisms between host immune receptors and pathogen effectors. Different alleles of immune receptors in the host display distinct recognition against pathogen effectors. The Piks allele of the rice immune receptor Pik does not recognize any known AvrPik variants, unlike other Pik alleles. The amino acid residue Glu229 in Piks-1 is critical for breaking the canonical Pik/AvrPik recognition pattern.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xinru Wu, Junjie Zhu, Liming Zhu, Yao Tang, Zhaodong Hao, Jingbo Zhang, Jisen Shi, Tielong Cheng, Lu Lu
Summary: In this study, 66 candidate proteins were identified from the halophyte Nitraria sibirica, and their potential involvement in abiotic stress and hormone responses was predicted. Transcriptome analysis revealed upregulation of certain NsCMLs under salt treatment and similarity in gene expression patterns under salt and cold stress. Overexpression of specific NsCMLs mitigated H2O2 accumulation caused by salt stress.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Qian Liu, Chunhong Zhang, Huiyong Fang, Letai Yi, Minhui Li
Summary: In a complex natural environment, plants have developed complex defense response mechanisms, including the NBS-LRR protein and alkaloids, to protect themselves. The NBS-LRR protein recognizes pathogenic microorganisms and triggers the immune response, while alkaloids inhibit pathogens. This study reviews the activation, recognition, and signal transduction of NBS-LRR proteins, as well as the synthesis and regulation of alkaloids in plant protection and discusses their current and future applications in biotechnology.
Article
Agronomy
Mark J. Quinton-Tulloch, Katherine A. Steele
Summary: Plant resistance genes drive immune responses in crops. This study aimed to predict candidate NLR R-genes in rice genomes, detect variation in these genes, and identify potential selectable markers. The analysis revealed clusters of R-genes and pseudogenes, as well as a higher frequency of SNPs and InDels in NLR R-gene candidates. The findings provide valuable insights for developing disease-resistant varieties in the future.
Article
Biochemistry & Molecular Biology
Jianzhong Huang, Xiaoqiu Wu, Zhiyong Gao
Summary: Ubiquitination plays a crucial role in regulating the turnover of nucleotide-binding site and leucine-rich repeat receptors (NLRs). BOI, a RING-type E3 ligase, has been found to interact with and ubiquitinate L5, leading to its proteasomal degradation outside the nucleus. BOI and its homologs, BRG1 and BRG3, function redundantly to negatively regulate the protein level of L5, demonstrating their multiple targets and subcellular functions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Plant Sciences
Yuchen Chen, Wei Wu, Bohan Yang, Fei Xu, Shanshan Tian, Jiang Lu, Peining Fu
Summary: The expression of VaRPP13 gene enhances resistance to oomycete pathogens in Arabidopsis and tobacco, and this enhancement is closely related to the activation of salicylic acid signaling pathway.
PLANT CELL REPORTS
(2022)
Article
Plant Sciences
Minxiang Yu, Zhuangzhi Zhou, Xue Liu, Dedong Yin, Dayong Li, Xianfeng Zhao, Xiaobing Li, Shengping Li, Renjie Chen, Ling Lu, Dewei Yang, Dingzhong Tang, Lihuang Zhu
Summary: Research on rice NLR proteins in response to blast infection revealed a signaling pathway involving OsSPK1, OsRac1, and RAI1, which may be conserved across different NLR-mediated disease resistances. Additionally, changes in RAI1 levels during infection led to the identification of OsRPT2a, suggesting a 26S proteasome-dependent turnover mechanism for RAI1. These findings indicate a potential common defense signaling route for NLR proteins in response to blast infection.
Article
Biotechnology & Applied Microbiology
Dan Du, Changwei Zhang, Yadi Xing, Xin Lu, Linjun Cai, Han Yun, Qiuli Zhang, Yingying Zhang, Xinlong Chen, Mingming Liu, Xianchun Sang, Yinghua Ling, Zhenglin Yang, Yunfeng Li, Benoit Lefebvre, Guanghua He
Summary: A mutation in the OsRLR1 gene in rice leads to broad-spectrum resistance to fungal and bacterial pathogens, accompanied by growth reduction; OsRLR1 mediates defense response through direct interaction with the transcription factor OsWRKY19 in the nucleus; OsWRKY19 activates defense response by binding to the promoter of OsPR10.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Qiguo Sun, Risheng Huang, Haifeng Zhu, Yanmei Sun, Zhenfei Guo
Summary: MtCML42 positively regulates the expression of MtCBF1 and MtCBF4, leading to upregulation of some COR genes, MtGolS1 and MtGolS2, resulting in raffinose accumulation and increased cold tolerance. Additionally, MtCML42 regulates flowering time through sequentially downregulating MtABI5 and upregulating MtFTa1 expression.
Article
Biochemistry & Molecular Biology
Yongwei Liu, Wenye Chen, Linbin Liu, Yuhuan Su, Yuan Li, Weizhe Jia, Bo Jiao, Jiao Wang, Fan Yang, Fushuang Dong, Jianfang Chai, He Zhao, Mengyu Lv, Yanyi Li, Shuo Zhou
Summary: Calmodulin (CaM) and calmodulin-like (CML) genes play important roles in plant growth and stress response. In wheat, 18 TaCaM and 230 TaCML genes were identified, classified into 7 subfamilies. These genes are located on all 21 chromosomes, with most gene clusters containing 3 homoeologous genes. TaCaM/TaCML genes show tissue-specific expression patterns, with some genes being up-regulated by abiotic stresses in roots or shoots.
PLANT SIGNALING & BEHAVIOR
(2022)
Article
Plant Sciences
Jun Yang, Shuang Liu, Lingxiao Ji, Xianying Tang, Yongsheng Zhu, Guosheng Xie
JOURNAL OF PLANT PHYSIOLOGY
(2020)
Article
Engineering, Multidisciplinary
Xinjie Yuan, Yuanchang Liu, Richard Bucknall
Summary: This study focuses on a novel combined energy system designed for the U.K. market, aiming to determine key parameters using objective sizing techniques to achieve maximum efficiency and minimum emissions.
IEEE TRANSACTIONS ON INDUSTRY APPLICATIONS
(2021)
Article
Plant Sciences
Pei Jing, Dongyan Kong, Lingxiao Ji, Lin Kong, Yanting Wang, Liangcai Peng, Guosheng Xie
Summary: This study elucidates the mechanism by which caleosin affects salt stress tolerance in rice, involving its interaction with OsDi19-5, the role of OsDi19-5 as a transcriptional repressor, and the regulation of salt stress-related target genes. These findings contribute to a deeper understanding of the molecular pathways involved in the salt stress response and have implications for enhancing salt tolerance in rice.
Article
Plant Sciences
Fangxi Wu, Xi Luo, Lingqiang Wang, Yidong Wei, Jianguo Li, Huaan Xie, Jianfu Zhang, Guosheng Xie
Summary: This study identified nine new QTLs associated with rice seed storability, shedding light on why the seed storability of indica subspecies is superior to japonica subspecies. The potentially co-localized genes with these QTLs could be targets for pyramiding breeding to improve seed storability in rice varieties.
Article
Thermodynamics
Xinjie Yuan, Yuanchang Liu, Richard Bucknall
Summary: This paper introduces a new algorithm for optimizing the operation of a novel SOFC-solar hybrid CCHP residential system. The algorithm combines MOPSO and GRA to maximize energy utilization efficiency and minimize system costs and emissions.
ENERGY CONVERSION AND MANAGEMENT
(2021)
Article
Biochemistry & Molecular Biology
Xiaoshuang Wei, Hailian Zhou, Deying Xie, Jianguo Li, Mingchong Yang, Tianli Chang, Dongxin Wang, Lihua Hu, Guosheng Xie, Jihong Wang, Lingqiang Wang
Summary: A new quantitative trait nucleotide (QTN) for rice shoot length, C/T, was identified in the OsWRKY21 gene, affecting plant height and internode elongation. Overexpressing this gene resulted in a semi-dwarf phenotype, while CRISPR/Cas9 knockout had the opposite effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Jun Yang, Lingxiao Ji, Shuang Liu, Pei Jing, Jin Hu, Deming Jin, Lingqiang Wang, Guosheng Xie
Summary: The OsCaM1-associated OsCCaMK-OsMKK1/6 cascade plays a critical role in recruiting auxin signaling in rice roots, affecting salt stress-related auxin signaling and lateral root growth. These findings provide new insight into the regulatory mechanism of CaM-mediated phosphorylation relay cascade to auxin signaling in lateral root growth under salt stress in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Plant Sciences
Xiaoshuang Wei, Shuang Liu, Cheng Sun, Guosheng Xie, Lingqiang Wang
Summary: This review summarizes the recent progress in understanding the cold stress response mechanisms of Arabidopsis and rice, highlighting both convergent and divergent mechanisms between the two species. Genetic engineering approaches for developing freezing tolerant Arabidopsis and chilling tolerant rice were discussed, along with future perspectives on cold stress signaling and tolerance in rice.
Article
Plant Sciences
Xiaocui Wang, Pengxu Ren, Lingxiao Ji, Bohua Zhu, Guosheng Xie
Summary: This study found that OsVDE negatively regulates ABA biosynthesis and salt stress tolerance in rice. Overexpression and gene-editing transgenic lines of OsVDE showed different phenotypes, with gene-editing lines being more sensitive to exogenous ABA and exhibiting higher ABA levels, stomatal closure percentage, and survival rate under salt stress. These results provide new evidence for the essential role of VDE in ABA biosynthesis and salt stress tolerance in plants.
Article
Energy & Fuels
Zhichao Gong, Bowen Wang, Yifan Xu, Meng Ni, Qingchen Gao, Zhongjun Hou, Jun Cai, Xin Gu, Xinjie Yuan, Kui Jiao
Summary: An adaptive optimization matching method is developed to maintain the high-efficiency operation of automotive polymer electrolyte membrane fuel cell (PEMFC) system. The system efficiency is optimized using genetic algorithm, and matching strategies for the compressor under different stack conditions are developed. It is found that the optimized method improves system efficiency.
Article
Biochemistry & Molecular Biology
Jianguo Li, Ahmed Adel Khatab, Lihua Hu, Liyan Zhao, Jiangyi Yang, Lingqiang Wang, Guosheng Xie
Summary: This study identified genes involved in cold stress and chilling accumulation in rice through GWAS, detecting a large number of SNPs and QTLs associated with cold stress and chilling acclimation. Additionally, new candidate genes were found, and genetic characteristics related to chilling acclimation were determined.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Ahmed Adel Khatab, Jianguo Li, Lihua Hu, Jiangyi Yang, Chuchuan Fan, Lingqiang Wang, Guosheng Xie
Summary: Associated analysis of GWAS with RNA-seq identified candidate genes responsible for cold stress and chilling acclimation in rice. Haplotypes of two candidate genes and their geographic distribution were analyzed. These findings provide new insights into the molecular mechanism and candidate genes for cold stress and chilling acclimation in rice.
Article
Plant Sciences
Jiajia Li, Dongyan Kong, Ting Song, Zhenzhu Hu, Qiang Li, Benze Xiao, Felix Kessler, Zhengfeng Zhang, Guosheng Xie
Summary: This study revealed that OsFBN7 interacts with OsKAS Ia/Ib enzymes and promotes the clustering of Plastoglobules (PG) in chloroplasts, leading to increased levels of lipid precursors and membrane lipids. Furthermore, OsFBN7 enhances the abundance and stability of OsKAS Ia/Ib, and upregulates the expression of genes involved in PG formation. These findings propose a new model for the regulation of chloroplast and PG membrane lipids by OsFBN7.
Article
Plant Sciences
Lingxiao Ji, Zhengfeng Zhang, Shuang Liu, Liyan Zhao, Qiang Li, Benze Xiao, Nobuhiro Suzuki, David J. Burks, Rajeev K. Azad, Guosheng Xie
Summary: This study explores the role of OsTIL1 lipocalin in maintaining cell membrane integrity under cold stress. The findings show that OsTIL1 positively regulates cold stress tolerance by protecting the cell membrane from oxidative damage and enhancing the activities of peroxidase and ascorbate peroxidase. OsTIL1 overexpression induces higher levels of 18:3-mediated glycerolipids and increases the transcription and enzyme abundance of four x-3 FADs under cold stress.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
