Article
Biochemistry & Molecular Biology
Sarah M. Smith, Gabrielle Larocque, Katherine M. Wood, Kyle L. Morris, Alan M. Roseman, Richard B. Sessions, Stephen J. Royle, Corinne J. Smith
Summary: The interaction between Clathrin and the AP2 complex plays a crucial role in coated-pit assembly, with a beta 2 appendage able to bind in at least two positions in the clathrin cage. This multi-modal binding is shown to be a fundamental property of clathrin-AP2 interactions.
Review
Cell Biology
Petia Adarska, Luis Wong-Dilworth, Francesca Bottanelli
Summary: Molecular switches of the ADP-ribosylation factor (ARF) GTPase family play crucial roles in coordinating intracellular trafficking, including membrane deformation, cargo sorting, and recruitment of downstream coat proteins and effectors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Yumiko Saito-Nakano, Takashi Makiuchi, Mami Tochikura, Carol A. Gilchrist, William A. Petri, Tomoyoshi Nozaki
Summary: The protist EhArfX2, involved in vesicular traffic regulation, is highly upregulated in a virulent strain of Entamoeba histolytica. Its function is essential for intracellular transportation and defense against reactive oxygen and nitrogen species, contributing to virulence in the host organism.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hsiang-Pu Feng, Hsiao-Yun Cheng, Ting-Feng Hsiao, Tai-Wei Lin, Jia-Wei Hsu, Lien-Hung Huang, Chia-Jung Yu
Summary: ADP-ribosylation factors (Arfs) and Arf-like (Arl) GTPases are crucial regulators of intracellular vesicle trafficking and Golgi structure. In this study, it was found that ArfGAP1 can promote GTP hydrolysis of Arl1, affecting its function at the trans-Golgi network (TGN).
Article
Cell Biology
Dilip Menon, Apoorva Bhapkar, Bhoomika Manchandia, Gitanjali Charak, Surabhi Rathore, Rakesh Mohan Jha, Arpita Nahak, Moumita Mondal, Mohyeddine Omrane, Akash Kumar Bhaskar, Lipi Thukral, Abdou Rachid Thiam, Sheetal Gandotra
Summary: Lipid droplets are important organelles that maintain cellular lipid balance by storing and delivering lipids. The small GTPase ARL8B has been found to mediate lipid droplet turnover in lysosomes, facilitating efficient lipid transfer between these organelles and potentially offering insights into inflammatory disorders.
Article
Cell Biology
Kana Fujibayashi, Joji Mima
Summary: Arf-family small GTPases play crucial roles in membrane trafficking and tethering processes. Arf6 can act as a membrane tether by physically linking distinct lipid bilayers, while Arf1 exhibits weaker membrane tethering capability under current experimental conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Lisa Redlingshofer, Frances M. Brodsky
Summary: The AP2 adaptor complex plays a central role in regulating clathrin-mediated endocytosis, serving as a key hub for protein interactions beyond cargo recognition and clathrin recruitment. It mediates clathrin coated pit maturation and couples lattice formation to membrane deformation. AP2 complements the attenuating role of clathrin light chain subunits in driving clathrin assembly and dynamic lattice rearrangement for budding.
CELLS & DEVELOPMENT
(2021)
Article
Multidisciplinary Sciences
Yang Chen, Shaoqin Hu, Xuanang Wu, Zhenli Xie, Yuan Wang, Bianbian Wang, Xiaopeng Li, Yingmei Pei, Yuhao Gu, Kai Huang, Jingxiao Huo, Anqi Wei, Cheng Bi, Zhe Lu, Qian Song, Huadong Xu, Xinjiang Kang, Shuli Shao, Jiangang Long, Jiankang Liu, Zhuan Zhou, Rong Huang, Zuying Chai, Changhe Wang
Summary: Exocytosis and endocytosis are tightly coupled processes. The primary Ca2+ sensor Syt1 plays bidirectional roles in this coupling by promoting small-sized endocytosis but inhibiting large-sized bulk endocytosis. Ca2+ binding ability is crucial for Syt1 to regulate both types of endocytic pathways.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Alexander Johnson, Dana A. Dahhan, Nataliia Gnyliukh, Walter A. Kaufmann, Vanessa Zheden, Tommaso Costanzo, Pierre Mahou, Monika Hrtyan, Jie Wang, Juan Aguilera-Servin, Daniel Van Damme, Emmanuel Beaurepaire, Martin Loose, Sebastian Y. Bednarek, Jiri Friml
Summary: This study identified the TPLATE complex as a key component in the unique plant endocytosis mechanism, playing a crucial role in mediating membrane bending against high-turgor pressure in plant cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Yongming Luo, Junpei Takagi, Lucas Alves Neubus Claus, Chao Zhang, Shigetaka Yasuda, Yoko Hasegawa, Junji Yamaguchi, Libo Shan, Eugenia Russinova, Takeo Sato
Summary: Protein ubiquitination is a crucial post-translational modification that regulates diverse cellular processes. In this study, two deubiquitinating enzymes, UBP12 and UBP13, were discovered to directly target the PM-localized brassinosteroid receptor BRI1 in Arabidopsis. They play essential roles in governing BRI1 abundance and degradation, and thereby regulate plant growth.
Review
Biochemistry & Molecular Biology
David Perrais
Summary: Dynamin is a major protein involved in endocytosis and plays a central role in cellular processes such as actin remodeling. It has been extensively studied through various methods and has been shown to be a remarkable mechano-GTPase. It is part of a complex protein and lipid network in cells and cannot function in isolation.
BIOSCIENCE REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Alazne Arrazola Sastre, Miriam Luque Montoro, Hadriano M. Lacerda, Francisco Llavero, Jose L. Zugaza
Summary: Rab and Arf GTPases play crucial roles in the pathogenesis and progression of neurodegenerative diseases, making them potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Jyoti Das, Mahak Tiwari, Deepa Subramanyam
Summary: Clathrin is a crucial cytosolic protein involved in intracellular trafficking, mitosis, and cell signaling. Comprising of heavy and light chains, it forms triskelions that polymerize into clathrin coated vesicles for membrane transport. Despite the well-documented role of heavy chains, the regulation of membrane traffic and cell signaling by clathrin light chains remains incompletely understood.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Madhumitha Narasimhan, Michelle Gallei, Shutang Tan, Alexander Johnson, Inge Verstraeten, Lanxin Li, Lesia Rodriguez, Huibin Han, Ellie Himschoot, Ren Wang, Steffen Vanneste, Judit Sanchez-Simarro, Fernando Aniento, Maciek Adamowski, Jiri Friml
Summary: This study reexamined the effects of auxin on PIN endocytic trafficking and found that high concentrations of NAA and IAA have nonspecific effects on the endomembrane system, while low concentrations specifically promoted endocytosis of PIN2. This contributes to polarity maintenance and highlights the nonspecific effects of high auxin levels on trafficking and endomembrane compartments.
Article
Cell Biology
Manuela Ecker, Gregory M. Redpath, Philip R. Nicovich, Jeremie Rossy
Summary: Endocytic trafficking plays a crucial role in controlling the density of molecules at the plasma membrane, influencing cell interaction with the environment. Understanding how proteins are endocytosed, sorted, and recycled is important for studying cellular processes. The use of photoactivation of fluorescent proteins enables quantitative analysis of endocytosis and intracellular transport.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Biochemistry & Molecular Biology
Akane Yoshida, Mikiko Shigekuni, Kenji Tanabe, Akikazu Fujita
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2016)
Article
Oncology
Yuji Henmi, Natsuko Oe, Nozomu Kono, Tomohiko Taguchi, Kohji Takei, Kenji Tanabe
EXPERIMENTAL CELL RESEARCH
(2016)
Article
Cell Biology
Yuji Henmi, Yoshiaki Morikawa, Natsuko Oe, Narumi Ikeda, Akikazu Fujita, Kohji Takei, Shane Minogue, Kenji Tanabe
MOLECULAR BIOLOGY OF THE CELL
(2016)
Article
Multidisciplinary Sciences
Kenji Tanabe
SCIENTIFIC REPORTS
(2016)
Review
Biochemistry & Molecular Biology
Kenji Tanabe
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Cell Biology
Kan-na Tomioku, Mikiko Shigekuni, Hiroki Hayashi, Akane Yoshida, Taiki Futagami, Hisanori Tamaki, Kenji Tanabe, Akikazu Fujita
EUROPEAN JOURNAL OF CELL BIOLOGY
(2018)
Article
Biochemical Research Methods
Kenji Tanabe, Ayane Inagaki, Yuji Henmi, Masanobu Satake
Article
Multidisciplinary Sciences
Shinya Rai, Hirokazu Tanaka, Mai Suzuki, Honami Ogoh, Yasuhiro Taniguchi, Yasuyoshi Morita, Takahiro Shimada, Akira Tanimura, Keiko Matsui, Takafumi Yokota, Kenji Oritani, Kenji Tanabe, Toshio Watanabe, Yuzuru Kanakura, Itaru Matsumura
Article
Cell Biology
Yuna Kurokawa, Akane Yoshida, Emi Fujii, Kanna Tomioku, Hiroki Hayashi, Kenji Tanabe, Akikazu Fujita
Article
Immunology
Shunsuke Kimura, Nobuhide Kobayashi, Yutaka Nakamura, Takashi Kanaya, Daisuke Takahashi, Ryoji Fujiki, Mami Mutoh, Yuuki Obata, Toshihiko Iwanaga, Tomoo Nakagawa, Naoya Kato, Shintaro Sato, Tsuneyasu Kaisho, Hiroshi Ohno, Koji Hase
JOURNAL OF EXPERIMENTAL MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Motoyoshi Nagai, Ryotaro Noguchi, Daisuke Takahashi, Takayuki Morikawa, Kouhei Koshida, Seiga Komiyama, Narumi Ishihara, Takahiro Yamada, Yuki Kawamura, Kisara Muroi, Kouya Hattori, Nobuhide Kobayashi, Yumiko Fujimura, Masato Hirota, Ryohtaroh Matsumoto, Ryo Aoki, Miwa Tamura-Nakano, Machiko Sugiyama, Tomoya Katakai, Shintaro Sato, Keiyo Takubo, Taeko Dohi, Koji Hase
Review
Immunology
Nobuhide Kobayashi, Daisuke Takahashi, Shunsuke Takano, Shunsuke Kimura, Koji Hase
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Shunsuke Kimura, Yutaka Nakamura, Nobuhide Kobayashi, Katsuyuki Shiroguchi, Eiryo Kawakami, Mami Mutoh, Hiromi Takahashi-Iwanaga, Takahiro Yamada, Meri Hisamoto, Midori Nakamura, Nobuyuki Udagawa, Shintaro Sato, Tsuneyasu Kaisho, Toshihiko Iwanaga, Koji Hase
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Akane Yoshida, Hiroki Hayashi, Kenji Tanabe, Akikazu Fujita
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2017)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)