Article
Biotechnology & Applied Microbiology
Olli Huhtinen, Rune Salbo, Urpo Lamminmaki, Stuart Prince
Summary: Mammalian display allows for the selection of favorable antibodies from a large antibody library displayed on mammalian cells. A new mammalian display platform was constructed and validated, using the Flp-In CHO cell line. This platform demonstrated the ability to select favorable antibodies and improve the biophysical properties of bococizumab.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ruiqi Luo, Baole Qu, Lili An, Yun Zhao, Yang Cao, Peng Ren, Haiying Hang
Summary: This study developed a thermo-resistant cell display system for improving both the stability and affinity of antibodies. By culturing and labeling cells at high temperatures, antibody mutants with the highest affinities and display levels were successfully enriched. The affinity and stability of three antibodies were simultaneously improved in the experiments.
BIOENGINEERING-BASEL
(2022)
Article
Medicine, General & Internal
Menglong Xu, Gaoxin Lei, Manman Chen, Ke Wang, Wenxiu Lv, Panpan Zhang, Tuo Hu, Jie Gao, Chenchen Lu, Ying Mei, Zhipan Xu, Zhengli Bai, Huajing Hu, Yiwei Jiang, Shuhua Tan
Summary: A fully human antibody FAP2M21 with potent inhibitory effect on PCSK9 was generated, showing promise as a therapeutic agent for treating hypercholesterolemia and associated cardiovascular diseases.
Article
Medicine, Research & Experimental
Yang Qin, Jiayi Jin, Jiani Zhang, Hui Wang, Li Liu, Yanwen Zhang, Sunwang Ling, Jinzhu Hu, Nuan Li, Jianguang Wang, Chen Lv, Xinyu Yang
Summary: Rheumatoid arthritis (RA) is a common chronic autoimmune disease for which traditional treatments often fall short. In this study, researchers developed a fully human SEMA5A-blocking antibody and demonstrated its effectiveness in inhibiting disease progression and improving disease characteristics in a mouse model of RA.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Ava E. Brent, Emily A. Buchholtz, Jennifer H. Mansfield
Summary: We examined the origins of the therian sternum using developmental, genetic, and paleontological data. Our findings showed that the therian sternum was assembled from pre-existing elements, namely the interclavicle and sternal bands. These elements articulates with the clavicle and ribs and were independent elements throughout most of synapsid history. The fusion of the interclavicle and anterior sternal bands to form a presternum was a unique innovation in therians.
Article
Allergy
Azadeh Hadadianpour, Jacob Daniel, Jian Zhang, Benjamin W. Spiller, Asta Makaraviciute, Asa M. DeWitt, Heather S. Walden, Robert G. Hamilton, R. Stokes Peebles, Thomas B. Nutman, Scott A. Smith
Summary: This study generated human hybridomas secreting naturally occurring helminth-specific IgE mAbs from individuals with filarial infections. The research provides valuable insight into the immune response and IgE antibody response induced by parasitic worms.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Immunology
Brian M. Petersen, Sophia A. Ulmer, Emily R. Rhodes, Matias F. Gutierrez-Gonzalez, Brandon J. Dekosky, Kayla G. Sprenger, Timothy A. Whitehead
Summary: Monoclonal antibodies are key therapeutics for cancer, inflammation, and infectious diseases. Natural human antibody repertoire-based scoring matrices can accurately predict mutations in therapeutic antibodies. High frequency mutations in natural human antibody repertoires have the potential to improve existing therapeutic antibodies and reduce immunogenicity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Brian L. L. Hie, Varun R. R. Shanker, Duo Xu, Theodora U. J. Bruun, Payton A. A. Weidenbacher, Shaogeng Tang, Wesley Wu, John E. E. Pak, Peter S. S. Kim
Summary: Natural evolution explores vast sequence space for rare mutations, and learning from it can guide artificial evolution. A study found that general protein language models can efficiently evolve human antibodies by suggesting evolutionarily plausible mutations, even without target antigen, binding specificity, or protein structure information. Language-model-guided affinity maturation improved the binding affinities of clinically relevant antibodies up to sevenfold and unmatured antibodies up to 160-fold, with favorable thermostability and viral neutralization activity. The models also guided efficient evolution across diverse protein families and selection pressures.
NATURE BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Damiano Buratto, Yue Wan, Xiaojie Shi, Guang Yang, Francesco Zonta
Summary: The steady increase in computational power has opened unprecedented opportunities in biology, as computer simulations can accurately reproduce experimental results and potentially replace expensive and technically tricky experiments. In this study, computer simulations were used to improve the biochemical characteristics of an antibody, and the results showed a good correlation between the simulated binding energies and the experimental binding affinities. The study also demonstrated the ability to design new antibody sequences with high affinity in silico, which could be obtained within a similar timeframe as traditional affinity maturation methods.
Article
Multidisciplinary Sciences
Nicole M. Wong, Elizabeth Frias, Frederic D. Sigoillot, Justin H. Letendre, Marc Hild, Wilson W. Wong
Summary: Cell-based transcriptional reporters are crucial in high-throughput screening, but many of them have weak or transient responses. This article introduces a digitizer circuit for amplifying reporter activity, increasing sensitivity, and retaining memory of pathway activation.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Nora Langreder, Dorina Schaeckermann, Doris Meier, Marlies Becker, Maren Schubert, Stefan Duebel, Thomas Reinard, Stefanie Figge-Wegener, Kristine Rossbach, Wolfgang Baeumer, Simone Ladel, Michael Hust
Summary: Insect bite hypersensitivity (IBH), the most common allergic skin disease in horses, is caused by bites from Culicoides spp. insects, resulting in a type I/IVb allergy involving eosinophil cells. A potential treatment option is the use of a therapeutic antibody targeting equine interleukin 5, the main activator and regulator of eosinophils. Through phage display and in vitro affinity maturation, an antibody named NOL226-2-D10 was developed, showing strong inhibition of interleukin 5 binding and satisfactory producibility. This antibody is a promising candidate for in vivo studies to treat equine IBH.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Roy A. Ehling, Cedric R. Weber, Derek M. Mason, Simon Friedensohn, Bastian Wagner, Florian Bieberich, Edo Kapetanovic, Rodrigo Vazquez-Lombardi, Raphael B. Di Roberto, Kai-Lin Hong, Camille Wagner, Michele Pataia, Max D. Overath, Daniel J. Sheward, Ben Murrell, Alexander Yermanos, Andreas P. Cuny, Miodrag Savic, Fabian Rudolf, Sai T. Reddy
Summary: This study presents a technology pipeline that combines single-cell antibody repertoire sequencing and mammalian display to investigate the specificity of plasma cells from convalescent COVID-19 patients. The results reveal specific antibodies targeting SARS-CoV-2 antigens, including those with high affinity and broad binding to the receptor binding domain (RBD) of SARS-CoV-2 variants.
Article
Biochemistry & Molecular Biology
Matthew G. Cyr, Henry D. Wilson, Anna-Lena Spierling, Jing Chang, Haiyong Peng, Peter Steinberger, Christoph Rader
Summary: This article introduces an unbiased approach to antibody discovery, which relies on generating monoclonal antibodies (mAbs) against native target cell surfaces via phage display. The authors demonstrated that this method efficiently identifies mAbs with the desired target cell reactivity and identified and validated three cell surface antigens in multiple myeloma. This study highlights the utility of optimized whole-cell phage display selection methods and their potential in target-unbiased antibody discovery workflows.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biology
Kiyomi Shitaoka, Akifumi Higashiura, Yohei Kawano, Akima Yamamoto, Yoko Mizoguchi, Takao Hashiguchi, Norihisa Nishimichi, Shiyu Huang, Ayano Ito, Shun Ohki, Miyuki Kanda, Tomohiro Taniguchi, Rin Yoshizato, Hitoshi Azuma, Yasuo Kitajima, Yasuyuki Yokosaki, Satoshi Okada, Takemasa Sakaguchi, Tomoharu Yasuda
Summary: Broadly neutralizing antibodies against SARS-CoV-2 variants, including Omicron, were found in long-term hospitalized convalescent patients infected with early SARS-CoV-2 strain B.1.1, and their mechanisms were identified through crystal structures. The decrease in antibody efficacy against mutated SARS-CoV-2 spike RBD explains breakthrough infections and reinfections by Omicron variants. Therefore, eliciting RBD-specific B cells in the longitudinal germinal center reaction provides potent immunity against emerging wide range of SARS-CoV-2 variants.
COMMUNICATIONS BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Opeyemi Ernest Oludada, Giulia Costa, Clare Burn Aschner, Anna S. Obraztsova, Katherine Prieto, Caterina Canetta, Stephen L. Hoffman, Peter G. Kremsner, Benjamin Mordmueller, Rajagopal Murugan, Jean-Philippe Julien, Elena A. Levashina, Hedda Wardemann
Summary: The central repeat and junction domain of Plasmodium falciparum circumsporozoite protein (PfCSP) were studied to guide malaria vaccine design using monoclonal antibodies (mAbs). The study describes the molecular characteristics and protective potential of 73 germline and mutated human mAbs against the highly immunogenic PfCSP C-terminal domain.
EMBO MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Shin-Ei Matsumoto, Yumiko Motoi, Koichi Ishiguro, Takeshi Tabira, Fuyuki Kametani, Masato Hasegawa, Nobutaka Hattori
HUMAN MOLECULAR GENETICS
(2015)
Article
Clinical Neurology
Yuko Hara, Yumiko Motoi, Keigo Hikishima, Hiroshi Mizuma, Hirotaka Onoe, Shin-Ei Matsumoto, Montasir Elahi, Hideyuki Okano, Shigeki Aoki, Nobutaka Hattori
CURRENT ALZHEIMER RESEARCH
(2017)
Article
Neurosciences
Hideo Hara, Fumiko Ono, Shinichiro Nakamura, Shin-ei Matsumoto, Haifeng Jin, Nobutaka Hattori, Takeshi Tabira
JOURNAL OF ALZHEIMERS DISEASE
(2016)
Article
Neurosciences
Montasir Elahi, Zafrul Hasan, Yumiko Motoi, Shin-Ei Matsumoto, Koichi Ishiguro, Nobutaka Hattori
JOURNAL OF ALZHEIMERS DISEASE
(2016)
Article
Neurosciences
Montasir Elahi, Yumiko Motoi, Shin-Ei Matsumoto, Zafrul Hasan, Koichi Ishiguro, Nobutaka Hattori
NEUROSCIENCE LETTERS
(2016)
Article
Biochemistry & Molecular Biology
Kosuke Tomimatsu, Shin-ei Matsumoto, Makiko Yamashita, Kiichiro Teruya, Yoshinori Katakura, Shigeru Kabayama, Sanetaka Shirahata
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2009)
Article
Biochemistry & Molecular Biology
Tsukasa Fujiki, Atsuji Tsuji, Shin-ei Matsumoto, Makiko Yamashita, Kiichiro Teruya, Sanetaka Shirahata, Yoshinori Katakura
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2010)
Article
Biotechnology & Applied Microbiology
Gakuro Harada, Shin-ei Matsumoto, Makiko Yamashita, Kaoru Fujii, Sanetaka Shirahata, Yoshinori Katakura
Article
Immunology
Makiko Yamashita, Gakuro Harada, Shin-ei Matsumoto, Yoshihiro Aiba, Akira Ichikawa, Tsukasa Fujiki, Miyako Udono, Shigeru Kabayama, Tadashi Yoshida, Pingbo Zhang, Hiroshi Fujii, Sanetaka Shirahata, Yoshinori Katakura
Article
Neurosciences
Tohru Hasegawa, Masayoshi Ichiba, Shin-ei Matsumoto, Koji Kasanuki, Taku Hatano, Hiroshige Fujishiro, Eizo Iseki, Nobutaka Hattori, Tatsuo Yamada, Takeshi Tabira
JOURNAL OF ALZHEIMERS DISEASE
(2012)
Article
Neurosciences
Satomi Shiota, Hidenori Takekawa, Shin-ei Matsumoto, Kazuya Takeda, Fariz Nurwidya, Yasuko Yoshioka, Fumiyuki Takahashi, Nobutaka Hattori, Takeshi Tabira, Hideki Mochizuki, Kazuhisa Takahashi
JOURNAL OF ALZHEIMERS DISEASE
(2013)
Article
Neurosciences
Kohei Shimada, Yumiko Motoi, Koichi Ishiguro, Taiki Kambe, Shin-ei Matsumoto, Masako Itaya, Miyuki Kunichika, Hideo Mori, Atsuko Shinohara, Momoko Chiba, Yoshikuni Mizuno, Takashi Ueno, Nobutaka Hattori
NEUROBIOLOGY OF DISEASE
(2012)
Article
Geriatrics & Gerontology
Sachiko Nakayama, Shotaro Shimonaka, Montasir Elahi, Kenya Nishioka, Yutaka Oji, Shin-Ei Matsumoto, Yuanzhe Li, Hiroyo Yoshino, Kaoru Mogushi, Taku Hatano, Takeshi Sato, Teikichi Ikura, Nobutoshi Ito, Yumiko Motoi, Nobutaka Hattori
NEUROBIOLOGY OF AGING
(2019)
Article
Biochemistry & Molecular Biology
Shotaro Shimonaka, Shin-Ei Matsumoto, Montasir Elahi, Koichi Ishiguro, Masato Hasegawa, Nobutaka Hattori, Yumiko Motoi
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)