Article
Biochemistry & Molecular Biology
Thomas Eekhout, Jose Antonio Pedroza-Garcia, Pooneh Kalhorzadeh, Geert De Jaeger, Lieven De Veylder
Summary: During DNA replication, the WEE1 kinase inhibits cyclin-dependent kinases (CDKs) to safeguard genomic integrity. Wee1 mutant plants are hypersensitive to replication stress due to their inability to respond properly to DNA replication problems. The pol alpha-2 mutant, with a mutated catalytic subunit of DNA polymerase alpha, acts as a suppressor mutant of wee1 by causing instability and interaction loss, leading to a prolonged S-phase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Luke A. Yates, Elias A. Tannous, R. Marc Morgan, Peter M. Burgers, Xiaodong Zhang
Summary: This study reveals the phosphorylation circuit that regulates checkpoint recruitment and function in response to DNA damage. The interaction between Ddc2 and RPA modulates the association between RPA and ssDNA, and phosphorylated Ddc2 enhances its recruitment to RPA-ssDNA, promoting DNA damage checkpoint signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Yaqun Liu, Xia Wu, Yves d'Aubenton-Carafa, Claude Thermes, Chun-Long Chen
Summary: During cell division, the activation of thousands of DNA replication origins ensures the complete duplication of the human genome. However, replication fork progression can be hindered by transcription-replication conflicts, with head-on conflicts being more detrimental. In this study, a bioinformatics toolkit called OKseqHMM was developed to analyze Okazaki fragment sequencing data and accurately predict replication initiation and termination, as well as replication fork directionality, in various organisms. This toolkit can greatly contribute to studying genome instability and its impact on human health.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Oncology
Nicola Lockwood, Silvia Martini, Ainara Lopez-Pardo, Katharina Deiss, Hendrika A. Segeren, Robert K. Semple, Ian Collins, Dimitra Repana, Mathias Cobbaut, Tanya Soliman, Francesca Ciccarelli, Peter J. Parker
Summary: G2 arrest is crucial for the faithful segregation of sister chromatids, and the p53-p21 signaling pathway plays an essential role in cell lines, patient-derived cells, and colorectal cancer organoids. In arrest-defective hTERT-positive cells, the PKC epsilon failsafe mechanism is engaged. In ALT-dependent cancer cells, a distinct form of p53-independent G2 arrest is mediated by BLM and Chk1.
Article
Multidisciplinary Sciences
Lorenza Garribba, Giuseppina De Feudis, Valentino Martis, Martina Galli, Marie Dumont, Yonatan Eliezer, Rene Wardenaar, Marica Rosaria Ippolito, Divya Ramalingam Iyer, Andrea E. Tijhuis, Diana C. J. Spierings, Michael Schubert, Silvia Taglietti, Chiara Soriani, Simon Gemble, Renata Basto, Nick Rhind, Floris Foijer, Uri Ben-David, Daniele Fachinetti, Ylli Doksani, Stefano Santaguida
Summary: Chromosomal instability (CIN) is a common form of genome instability and is closely associated with cancer. Aneuploidy, a state of karyotype imbalance, can trigger CIN and result in genetically diverse cells with chromosomal abnormalities. Cycling aneuploid cells have lower karyotype complexity compared to arrested ones, but both show increased expression of DNA repair signatures. Interestingly, highly-proliferative cancer cells also exhibit upregulation of the same signatures, which allows them to proliferate despite aneuploidy-induced CIN.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Lilas Courtot, Elodie Bournique, Chrystelle Maric, Laure Guitton-Sert, Miguel Madrid-Mencia, Vera Pancaldi, Jean-Charles Cadoret, Jean-Sebastien Hoffmann, Valerie Bergoglio
Summary: This study reveals that low replication stress can lead to advanced DNA replication timing, which is cell-type specific and involves large heterochromatin domains. These advanced events can be inherited by the next generation of cells, leading to changes in chromatin accessibility, replication origin landscape, and gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shuangyan Yao, Yuting Feng, Yan Zhang, Jinrong Feng
Summary: Cells face constant genetic assaults that cause DNA damage, leading to genome instability. Eukaryotic cells have evolved a DNA damage response system to monitor and repair these lesions. Studies show that DDR genes in pathogenic fungus Candida albicans are functionally similar to those in Saccharomyces cerevisiae, but may act through distinct mechanisms, particularly in resisting DNA damage stress induced by reactive oxygen species.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Genetics & Heredity
Luke A. Yates, Xiaodong Zhang
Summary: Yeast Mec1 and its mammalian ortholog Ataxia-Telangiectasia and Rad3-related are large protein kinases that play important roles in replication stress and double strand DNA break repair. Mec1ATR acts as a sensor of single stranded DNA in complex with Ddc2ATRIP, phosphorylating various cell cycle and DNA repair factors to induce cell cycle arrest and facilitate repair processes. Recent advancements in structural biology have shed light on the molecular mechanisms and regulation of Mec1ATR function in the DNA damage checkpoint.
Editorial Material
Cell Biology
Jean-Sebastien Hoffmann
Summary: The major challenge in DNA replication is to ensure the transmission of intact genetic material to daughter cells. Despite extensive research on the cellular responses to replication problems and their source, the transmission of genome modifications and their heritability in mitosis has been less documented. Recent studies have shown that low replication stress can impact the next generation of cells, transmitting DNA damage and altering the replication timing program of chromosomal domains in daughter cells. This progression of replication issues into mitosis and daughter cells may provide advantages by alerting cells to risky loci and offering an adaptive mechanism to anticipate future problems, especially in the context of cancer cell resistance to therapy.
Article
Virology
Tareq Abualfaraj, Nafiseh Chalabi Hagkarim, Robert Hollingworth, Laura Grange, Satpal Jhujh, Grant S. Stewart, Roger J. Grand
Summary: The Ad12E1B55K protein affects cellular DNA damage response pathways and DNA replication by interacting with multiple proteins, leading to genomic instability.
Article
Biochemistry & Molecular Biology
Sabrina X. Van Ravenstein, Kavi P. Mehta, Tamar Kavlashvili, Jo Ann W. Byl, Runxiang Zhao, Neil Osheroff, David Cortez, James M. Dewar
Summary: Using Xenopus egg extracts, this study demonstrates that the TOP2 poisons etoposide and doxorubicin inhibit DNA replication through different mechanisms. Etoposide induces TOP2-dependent DNA breaks and TOP2-dependent fork stalling, while doxorubicin stalls replication forks independently of TOP2 by intercalating into parental DNA.
Review
Pharmacology & Pharmacy
Nitasha Gupta, Tzu-Ting Huang, Sachi Horibata, Jung-Min Lee
Summary: Poly (ADP-ribose) polymerase (PARP) inhibitors have become a mainstay of therapy in ovarian cancer and other malignancies. However, an increasing number of patients develop resistance to PARP inhibitors, necessitating a deeper understanding of the mechanisms of resistance and the development of effective treatment strategies.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Biology
Iraia Garcia-Santisteban, Alba Llopis, Lenno Krenning, Jon Vallejo-Rodriguez, Bram van den Broek, Ana M. Zubiaga, Rene H. Medema
Summary: ATM becomes dispensable for G1 checkpoint maintenance as early as 1 hour after DSB induction, while CHK2 kinase activity is necessary to maintain G1 arrest independently of other proteins, suggesting that the G1 arrest is sustained in a lesion-independent manner.
Article
Biochemistry & Molecular Biology
Saman Khan, Nafees Ahamad, Sankhadip Bhadra, Zheng Xu, Yong-jie Xu
Summary: The Smc5/6 complex is essential for maintaining chromosome structure and genome stability. Two previously uncharacterized mutants of Smc5/6 were identified through screening for new DNA replication checkpoint mutants in fission yeast. The study found that SUMOylation of the RecQ helicase Rqh1 by Nse2 facilitates checkpoint signaling at the replication fork.
MOLECULAR AND CELLULAR BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Elias A. Tannous, Peter M. Burgers
Summary: DNA replication is a precise process, but cells face obstacles such as replication blocks and lack of precursors that can lead to chromosome instability and mutations. The PIKK family plays a crucial role in the cell's response mechanism, with recent studies showing significant progress in understanding the structure and activity of human ATR and ATM.
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Lu Gong, Hongjian Gong, Xiao Pan, Changqing Chang, Zhao Ou, Shengfan Ye, Le Yin, Lina Yang, Ting Tao, Zhenhai Zhang, Cong Liu, David P. Lane, Jinrong Peng, Jun Chen
Article
Multidisciplinary Sciences
Ming Zeng, Laifeng Ren, Ken'Ichi Mizuno, Konstantinos Nestoras, Haibin Wang, Zizhi Tang, Liandi Guo, Daochun Kong, Qiwen Hu, Qun He, Lilin Du, Antony M. Carr, Cong Liu
NATURE COMMUNICATIONS
(2016)
Article
Oncology
Zizhi Tang, Juan Yang, Xin Wang, Ming Zeng, Jing Wang, Ao Wang, Mingcai Zhao, Liandi Guo, Cong Liu, Dehua Li, Jie Chen
Article
Biochemistry & Molecular Biology
Liandi Guo, Xiaobo Wang, Laifeng Ren, Ming Zeng, Si Wang, Yuding Weng, Zizhi Tang, Xin Wang, Yaxiong Tang, Huaidong Hu, Mingyuan Li, Chongjie Zhang, Cong Liu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2014)
Article
Biochemical Research Methods
Xiaoqing Ren, Xue Bai, Xuefei Zhang, Zheyi Li, Lingfang Tang, Xuyang Zhao, Zeyang Li, Yanfei Ren, Shicheng Wei, Qingsong Wang, Cong Liu, Jianguo Ji
MOLECULAR & CELLULAR PROTEOMICS
(2015)
Article
Gastroenterology & Hepatology
Laifeng Ren, Ming Zeng, Zizhi Tang, Mingyuan Li, Xiaojun Wang, Yang Xu, Yuding Weng, Xiaobo Wang, Huan Wang, Liandi Guo, Bing Zuo, Xin Wang, Si Wang, Jiangyan Lou, Yaxiong Tang, Dezhi Mu, Ning Zheng, Xianhui Wu, Junhong Han, Antony M. Carr, Penelope Jeggo, Cong Liu
Article
Cell Biology
Xiaoyan Hu, Yang Meng, Lian Xu, Lei Qiu, Mingtian Wei, Dan Su, Xu Qi, Ziqiang Wang, Shengyong Yang, Cong Liu, Junhong Han
CELL DEATH & DISEASE
(2019)
Article
Biochemistry & Molecular Biology
Xuan Zheng, Yinghui Jia, Lei Qiu, Xinyi Zeng, Liangliang Xu, Mingtian Wei, Canhua Huang, Cong Liu, Liangyi Chen, Junhong Han
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Ming Zeng, Zizhi Tang, Liandi Guo, Xiaojun Wang, Cong Liu
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2020)
Article
Oncology
Liang Song, Zizhi Tang, Changsheng Peng, Yueming Yang, Chang Guo, Danqing Wang, Liandi Guo, Jie Chen, Cong Liu
Article
Oncology
Tianyu Miao, Changsheng Peng, Zizhi Tang, Ming Zeng, Shi Wang, Xiaojun Wang, Liandi Guo, Xiaobo Wang, Jichun Zhao, Mingcai Zhao, Jie Chen, Cong Liu
Summary: This study reveals that cancer cells subjected to cytotoxic treatments undergo significant changes in gene expression that promote cell mobility. This enhancement of metastatic potential is mainly dependent on the action of the ATM kinase, with ATM-activated cancer cells showing increased metastatic potential in ovarian cancer tissues.
Article
Multidisciplinary Sciences
Zizhi Tang, Ming Zeng, Xiaojun Wang, Chang Guo, Peng Yue, Xiaohu Zhang, Huiqiang Lou, Jun Chen, Dezhi Mu, Daochun Kong, Antony M. Carr, Cong Liu
Summary: The atypical nuclease ENDOD1 plays a role in innate immunity and has been found to have functions in DNA repair. Loss of ENDOD1 function is synthetic lethal with homologous recombination defects and also shows synthetic lethality with p53 mutation, making it a potential target for cancer treatment.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Chang Guo, Liandi Guo, Changsheng Peng, Yuegai Jia, Yueming Yang, Xiaojun Wang, Ming Zeng, Danqing Wang, Cong Liu, Mingcai Zhao, Jie Chen, Zizhi Tang
Summary: This study reveals the association between endogenous nucleolar stress and perturbed function of p53 in ovarian cancer.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jiaxin Zhang, Lili Li, Yu Miao, Xiaojing Liu, Haitao Sun, Meiqian Jiang, Xiaoli Li, Zhen Li, Cong Liu, Baohua Liu, Xingzhi Xu, Qinhong Cao, Wenya Hou, Chunlai Chen, Huiqiang Lou
Summary: In addition to entrapping sister chromatids, cohesin also drives other high-order chromosomal structural dynamics such as looping, compartmentalization, and condensation. ESCO2 acetylates a specific subset of cohesin to establish cohesion between nascent sister chromatids. The precise mechanism of this process is still unknown. This study reveals that GSK3 family kinases provide higher hierarchical control through an ESCO2 regulator, CRL4(MMS22L), by phosphorylating Thr105 in MMS22L and promoting the dimerization of CRL4(MMS22L) and ESCO2 during S phase. This research helps understand the symmetric control of cohesion establishment in eukaryotes.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jingjing Zhang, Di Shi, Xiaoli Li, Lin Ding, Jun Tang, Cong Liu, Katsuhiko Shirahige, Qinhong Cao, Huiqiang Lou
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
