Article
Endocrinology & Metabolism
Patrick A. Fletcher, Ben Thompson, Chante Liu, Richard Bertram, Leslie S. Satin, Arthur S. Sherman
Summary: The standard model for Ca2+ oscillations in insulin-secreting pancreatic 13 cells relies on voltage-activated Ca2+ channels for Ca2+ entry. These channels work together with ATP-dependent K+ channels to regulate the secretion of insulin in response to the metabolic state of the cells. However, an alternative model proposes that calcium-induced calcium release from the endoplasmic reticulum is the key driver of islet oscillations. This study shows that the alternative model is incompatible with established experimental data and that the standard model provides a better explanation for the new observations.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2023)
Article
Biology
Xin Shen, Jonas van den Brink, Anna Bergan-Dahl, Terje R. Kolstad, Einar S. Norden, Yufeng Hou, Martin Laasmaa, Yuriana Aguilar-Sanchez, Ann P. Quick, Emil K. S. Espe, Ivar Sjaastad, Xander H. T. Wehrens, Andrew G. Edwards, Christian Soeller, William E. Louch
Summary: This study investigates the promotion of RyR cluster dispersion by prolonged beta-adrenergic stimulation in heart failure, and examines the underlying mechanisms. The findings demonstrate the intimate connection between RyR localization and function through channel phosphorylation by CaMKII and PKA, which plays a protective role in healthy cardiomyocytes but contributes to impaired cardiac function during heart failure.
Article
Biochemistry & Molecular Biology
Gyula Diszhazi, Zsuzsanna E. Magyar, Erika Lisztes, Edit Toth-Molnar, Peter P. Nanasi, Rudi Vennekens, Balazs Toth, Janos Almassy
Summary: In this study, the TRPM4 current in pancreatic acinar cells was identified and its role in regulating cell membrane depolarization and Ca2+ signaling was demonstrated using pharmacological tools and TRPM4-deficient mice. The TRPM4 current was found to depolarize PACs and reduce the inward driving force for Ca2+, serving as a negative feedback regulator of Ca2+ entry.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Sang Youn Hwang, Hongqun Liu, Samuel S. Lee
Summary: Cirrhotic cardiomyopathy is a syndrome characterized by weakened cardiac systolic and diastolic function in patients with cirrhosis. The calcium handling system, including calcium channels, intracellular calcium-regulatory apparatus, and pertinent proteins, plays a crucial role in the cardiac function in cirrhotic cardiomyopathy. Multiple defects in calcium handling contribute to the pathogenesis of cirrhotic cardiomyopathy, including the reduction of L-type calcium channels and calcium leakage in cirrhotic cardiomyocytes. The decreased calcium storage in the sarcoplasmic reticulum leads to decreased cardiac contractility.
Article
Pharmacology & Pharmacy
Guillaume Daziano, Nicolas Blondeau, Sophie Beraud-Dufour, Amar Abderrahmani, Carole Rovere, Catherine Heurteaux, Jean Mazella, Patricia Lebrun, Thierry Coppola
Summary: The study demonstrates that PE and its derivatives can protect beta-cells against death induced by inflammation by inducing a rise of intracellular calcium concentration. In addition, Mini-Spadin promotes beta-cell proliferation, suggesting a possible regenerative effect. The findings highlight the potential roles of PE and its derivatives as pharmacological tools against diabetes.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Alejandro Pizzoni, Xuefeng Zhang, Nyla Naim, Daniel L. Altschuler
Summary: cAMP, traditionally thought to originate solely from the plasma membrane, can also be generated from internalized GsPCRs. In this study, the endogenously expressed TSHR was found to trigger internalization-dependent sAC activation, leading to PKA activation and cell proliferation. Inhibition of nuclear sAC impaired nuclear cAMP accumulation, PKA activation, and cell proliferation, while increased expression of nuclear sAC enhanced cell proliferation. These findings suggest a novel three-wave model in which the third wave of cAMP, generated by nuclear sAC, is sufficient and rate-limiting for thyroid cell proliferation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Heidi Espadas-Alvarez, Jacqueline Martinez-Rendon, Isabel Larre, Arturo Matamoros-Volante, Tatiana Romero-Garcia, Tamara Rosenbaum, Angelica Rueda, Refugio Garcia-Villegas
Summary: TRPV4 channel functions in the nucleus, inhibiting the transcriptional activity of beta-catenin through a direct interaction, and may regulate differentiation in various cellular contexts.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Cell Biology
Giuseppe Chiantia, Enis Hidisoglu, Andrea Marcantoni
Summary: Research into the early impacts of Alzheimer's disease on synapse function is a promising approach to finding a treatment. Recent studies have shown that the accumulation of Abeta42 peptide, which arises from the processing of APP, targets RyRs in hippocampal neurons, leading to the release of calcium from the ER and subsequent dysregulation of calcium concentration. This dysregulation is a hallmark of Alzheimer's disease and other neurodegenerative diseases. This review aims to summarize the current understanding of the role of RyRs in neuronal function under physiological conditions and during the onset of Alzheimer's disease.
Review
Cell Biology
Petronel Tuluc, Tamara Theiner, Noelia Jacobo-Piqueras, Stefanie M. Geisler
Summary: Pancreatic islets secrete hormones critical for glucose homeostasis, with insulin from beta-cells being the main hormone for lowering blood glucose levels. Glucagon and somatostatin also play important roles in regulating hormone secretion. High voltage-gated Ca2+ channels are central in the release of pancreatic hormones and play a key role in diabetes.
Review
Biochemistry & Molecular Biology
Razik Bin Abdul Mu-u-min, Abdoulaye Diane, Asma Allouch, Heba H. Al-Siddiqi
Summary: This review emphasizes the importance of calcium and its signaling pathways in beta cell function and the implications of calcium dysfunction in the onset of diabetes. Human pluripotent stem cell (hPSC) technology holds promise in generating functional pancreatic beta cells to replace dysfunctional cells, reducing the burden of diabetes. However, current differentiation protocols result in beta-like cells with limited functionality compared to adult beta cells. Targeting calcium function in differentiation protocols is suggested to improve the generation of mature and functional beta-like cells.
Article
Multidisciplinary Sciences
Kyle E. Harvey, Shiqi Tang, Emily K. LaVigne, Evan P. S. Pratt, Gregory H. Hockerman
Summary: In this study, the researchers found that RyR2 is required for the regulation of insulin content and glucose-stimulated insulin secretion in INS-1 cells, partly through its interaction with IRBIT. The experiments showed that the absence of RyR2 led to a decrease in store-operated Ca2+ entry and PLC activity, as well as an increase in cellular PIP2 levels. Furthermore, RyR2 was found to regulate beta-cell electrical activity by controlling Ca-v current density and SK channel activation.
Article
Multidisciplinary Sciences
Yulong Gong, Bingyuan Yang, Dingdong Zhang, Yue Zhang, Zihan Tang, Liu Yang, Katie C. Coate, Linlin Yin, Brittney A. Covington, Ravi S. Patel, Walter A. Siv, Katelyn Sellick, Matthew Shou, Wenhan Chang, E. Danielle Dean, Alvin C. Powers, Wenbiao Chen
Summary: Insufficient glucagon signalling leads to hyperaminoacidemia and stimulates the proliferation of glucagon-producing alpha cells. The study reveals that the amino acid sensitive calcium sensing receptor (CaSR) is necessary for alpha cell proliferation through Gq signalling during hyperaminoacidemia.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Anna Weiser, Jerome N. Feige, Umberto De Marchi
Summary: Accumulation of calcium in energized mitochondria of pancreatic beta-cells plays a crucial role in beta-cell function. Mitochondrial calcium signaling is important for regulating insulin secretion and new pharmacological and genetic findings provide potential intervention strategies for modulating pancreatic beta-cell function in the context of diabetes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
H. M. York, A. Patil, U. K. Moorthi, A. Kaur, A. Bhowmik, G. J. Hyde, H. Gandhi, A. Fulcher, K. Gaus, S. Arumugam
Summary: The endosomal system plays a crucial role in processing responses to growth factor receptors, with EGF activating EGFR and causing redistribution and movement of related proteins. Research shows that endosomal trafficking forms an efficient and fast-acting network to coordinate the movement of activated EGF receptors.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Medicinal
Jesus M. Aizpurua, Jose Miranda, Aitziber Irastorza, Endika Torres, Maite Eceiza, Maialen Sagartzazu-Aizpurua, Pablo Ferron, Garazi Aldanondo, Haizpea Lasa-Fernandez, Pablo Marco-Moreno, Naroa Dadie, Adolfo Lopez de Munain, Ainara Vallejo-Illarramendi
Summary: The study investigated the hypothesis of rescuing FKBP12/RyR1 interaction and intracellular calcium homeostasis through molecular reshaping of FKBP12. Novel triazoles were designed and synthesized, showing potential as therapeutic candidates for muscle disorders with nitro-oxidative stress, FKBP12/RyR1 dysfunction, and calcium dysregulation.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)