Article
Biochemistry & Molecular Biology
Euitaek Jung, Sukjin Ou, Sung Shin Ahn, Hyunjin Yeo, Young Han Lee, Soon Young Shin
Summary: In the tumor microenvironment, tumor necrosis factor alpha (TNF-alpha) promotes the endothelial differentiation of mesenchymal stem cells (MSCs) through the activation of the JNK-EGR1 signaling axis. This finding is important for identifying potential therapeutic targets for solid tumor vasculatures.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Moon-Young Song, Da-Young Lee, Sun-Mi Yun, Eun-Hee Kim
Summary: Glucose transporter 3 (GLUT3) is highly expressed in colorectal cancer (CRC) and promotes epithelial-mesenchymal transition (EMT) through the TGF-beta/JNK/ATF2 signaling pathway, enhancing invasive ability.
Article
Oncology
Milad Soleimani, Alexander Somma, Tamer Kaoud, Ria Goyal, Jorge Bustamante, Dennis C. Wylie, Nisha Holay, Agnieszka Looney, Uma Giri, Todd Triplett, Kevin Dalby, Jeanne Kowalski, S. Gail Eckhardt, Carla Van Den Berg
Summary: JNK-IN-8, a covalent JNK inhibitor, suppresses the growth of triple-negative breast cancer (TNBC) by activating TFEB/TFE3 and inducing lysosome biogenesis and autophagy via mTOR inhibition independently of JNK.
MOLECULAR CANCER THERAPEUTICS
(2022)
Article
Multidisciplinary Sciences
Glenn J. Markov, Thach Mai, Surag Nair, Anna Shcherbina, Yu Xin Wang, David M. Burns, Anshul Kundaje, Helen M. Blau
Summary: The AP-1 transcription factor c-Jun plays a critical role in human somatic cell reprogramming by enhancing enhancer accessibility in fibroblasts and repressing OCT4 expression. Through epigenome remodeling, successful reprogramming of human fibroblasts to induced pluripotent stem cells was achieved.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Ethan L. Morgan, James A. Scarth, Molly R. Patterson, Christopher W. Wasson, Georgia C. Hemingway, Diego Barba-Moreno, Andrew Macdonald
Summary: The study identified activation of the JNK signaling pathway in cervical disease and cancer, with the HPV E6 oncogene inducing JNK1/2 phosphorylation. Blocking JNK signaling reduced cell proliferation and induced apoptosis, while promoting EGFR signaling. This positive feedback loop between EGFR signaling and HPV E6/E7 expression may offer a novel therapeutic target for cervical cancer treatment.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Pharmacology & Pharmacy
Rajvir Singh, Ravinder K. Kaundal, Baoyin Zhao, Rihab Bouchareb, Djamel Lebeche
Summary: This study reveals the role of resistin in cardiac fibrosis, promoting fibroblast-to-myofibroblast differentiation through JAK/STAT3 and JNK/c-Jun pathways, independent of TGF beta 1. Resistin-null mice showed improvements in cardiac function and reduction in tissue fibrosis, indicating the potential stimulation of cardiac fibrosis by resistin.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Pei Xie, Li-Jun Yan, Hong-Ling Zhou, Hui-Hui Cao, Yuan-Ru Zheng, Zi-Bin Lu, Hua-Yi Yang, Jia-Mei Ma, Yu-Yao Chen, Chuying Huo, Chunyang Tian, Jun-Shan Liu, Lin-Zhong Yu
Summary: This study found that emodin protects against LPS-induced acute lung injury (ALI) by regulating the JNK/Nur77/c-Jun signaling pathway. The results of this study indicate the potential of emodin in the treatment of ALI.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Fang Liu, Yan Liu, Qifeng Peng, Guodong Wang, Qing Tan, Zhongyue Ou, Qishan Xu, Chixiang Liu, Daming Zuo, Jianbo Zhao
Summary: This study revealed that creatinine is closely related to drug-induced liver injury and exacerbates APAP-induced hepatocyte death by promoting the production and activation of reactive oxygen species and c-Jun N-terminal kinase.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Physiology
Yizhen Sang, Kenji Tsuji, Kazuhiko Fukushima, Kensaku Takahashi, Shinji Kitamura, Jun Wada
Summary: Renal fibrosis, a common pathological process in renal diseases, was studied using a UUO mouse model, revealing increased SEMA3A signaling and its inhibition ameliorating fibrosis through JNK regulation. This study suggests SEMA3A inhibitor as a potential therapeutic option for renal fibrosis.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jun Yang, Juan Wang, Yu Zhang, Wenjing Huang, Shaoqing Zhang, Peihao Yin, Wenfu Tan
Summary: In colorectal cancer, PGE2-JNK can activate the Hh signaling pathway non-canonically. This study demonstrates that c-Jun prevents Gli2 protein from degradation by protease through phosphorylation by PGE2. Suppression of c-Jun decreases Gli2 expression and ubiquitination, and restricts PGE2-induced Hh activity and colorectal cancer cell proliferation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Lesley Jia Wei Pua, Chun-Wai Mai, Felicia Fei-Lei Chung, Alan Soo-Beng Khoo, Chee-Onn Leong, Wei-Meng Lim, Ling-Wei Hii
Summary: JNK and p38 MAPK signaling pathways play important roles in nasopharyngeal carcinoma (NPC), promoting tumor processes such as cell proliferation, apoptosis, inflammation, metastasis, and angiogenesis. Although the underlying mechanisms are not fully understood, further research on JNK and p38 MAPK could provide important insights for diagnosis, prognosis, and treatment decision-making in NPC patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jolanta Chmielowiec, Wojciech J. Szlachcic, Diane Yang, Marissa A. Scavuzzo, Katrina Wamble, Alejandro Sarrion-Perdigones, Omaima M. Sabek, Koen J. T. Venken, Malgorzata Borowiak
Summary: In this study, researchers demonstrate that human pluripotent stem cell derived pancreatic progenitors can differentiate into insulin-secreting cells through the crosstalk of WNT5A and BMP signaling. They also identify a specific microenvironment during fetal pancreas development that allows beta-cell differentiation. They further reveal the important roles of WNT5A and endothelial-derived Endocan in promoting the in vitro development of beta-cells.
NATURE COMMUNICATIONS
(2022)
Article
Environmental Sciences
Yang Yang, Yajing Feng, Hui Huang, Liuxin Cui, Fuqin Li
Summary: In this study, PM2.5 exposure was found to have negative effects on sperm quality and serum testosterone levels in male rats, while the IRE1 inhibitor STF083010 showed significant improvement in these effects. These findings suggest that the reproductive injury induced by PM2.5 may be mediated through the IRE1/JNK/autophagy signaling pathway.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Pharmacology & Pharmacy
Yuwei Jiang, Jiaoya Xu, Ping Huang, Lili Yang, Yang Liu, Yiping Li, Jue Wang, Haiyan Song, Peiyong Zheng
Summary: This study confirms that mitochondrial dysfunction induced by the JNK/Sab signaling pathway plays a crucial role in the development of NASH. Scoparone, by suppressing this signaling pathway, can partially improve the lipotoxic liver injury, making it a potential therapeutic compound for NASH.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Yijing Ren, Cheng Lv, Jing Zhang, Beibei Zhang, Bei Yue, Xiaoping Luo, Zhilun Yu, Hao Wang, Junyu Ren, Zhengtao Wang, Wei Dou
Summary: Alantolactone (ATL) exhibits inhibitory effects on cell activities, induces apoptosis, and inhibits colony formation in colorectal cancer. It also reduces cell migration and invasion capabilities. Furthermore, ATL demonstrates significant anti-tumor effects in vivo models.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Cristian Prieto-Garcia, Oliver Hartmann, Michaela Reissland, Thomas Fischer, Carina R. Maier, Mathias Rosenfeldt, Christina Schuelein-Voelk, Kevin Klann, Reinhard Kalb, Ivan Dikic, Christian Munch, Markus E. Diefenbacher
Summary: Squamous cell carcinomas (SCC) exhibit high mutational burden and platinum-based chemotherapy resistance, with increased Np63 expression associated with resistance; USP28 is recruited to sites of DNA damage and plays a regulatory role in DNA damage repair in SCC; Targeting the USP28- increment Np63 axis can overcome chemotherapy resistance in SCC.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Chemistry, Multidisciplinary
Iris Hansjosten, Masanari Takamiya, Juliane Rapp, Luisa Reiner, Susanne Fritsch-Decker, Dorit Mattern, Silvia Andraschko, Chantal Anders, Giuseppina Pace, Thomas Dickmeis, Ravindra Peravali, Sepand Rastegar, Uwe Straehle, I. -Lun Hsiao, Douglas Gilliland, Isaac Ojea-Jimenez, Selina V. Y. Ambrose, Marie-France A. Belinga-Desaunay-Nault, Abdullah O. Khan, Iseult Lynch, Eugenia Valsami-Jones, Silvia Diabate, Carsten Weiss
Summary: The increasing use of manufactured nanomaterials in consumer products and industrial applications has raised concerns about potential impacts on human and ecosystem health. There is an urgent need for high throughput testing in nanotoxicology to predict hazards. Zebrafish embryos have emerged as a valuable model organism for studying nanotoxicity due to their characteristics for microscopy-based screening.
ENVIRONMENTAL SCIENCE-NANO
(2022)
Article
Cell Biology
Laura Mainz, Mohamed A. F. E. Sarhan, Sabine Roth, Ursula Sauer, Charis Kalogirou, Markus Eckstein, Elena Gerhard-Hartmann, Helen-Desiree Seibert, Hans-Ulrich Voelker, Carol Geppert, Andreas Rosenwald, Martin Eilers, Almut Schulze, Markus Diefenbacher, Mathias T. Rosenfeldt
Summary: The potential therapeutic target of macroautophagy/autophagy for various diseases, including cancer, is mainly based on preclinical mouse studies. This study introduces a shRNA transgenic mouse model that allows simultaneous knockdown of Atg7 in most organs, providing a valuable tool for further understanding of the role of autophagy impairment at different disease stages.
Article
Biochemistry & Molecular Biology
Stefan Weinschenk, Carsten Weiss, Justus Benrath, Volker von Baehr, Thomas Strowitzki, Manuel Feisst
Summary: This study investigated the effects of four local anesthetics on TNF-alpha secretion in human leucocytes. The results showed that all four local anesthetics reduced TNF-alpha secretion and can be potential candidates for treating overactive immune reactions and chronic inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Cristian Prieto-Garcia, Oliver Hartmann, Michaela Reissland, Fabian Braun, Suleyman Bozkurt, Nikolett Pahor, Carmina Fuss, Andreas Schirbel, Christina Schuelein-Voelk, Alexander Buchberger, Marco A. Calzado Canale, Mathias Rosenfeldt, Ivan Dikic, Christian Muench, Markus E. Diefenbacher
Summary: The oncogenic transformation of lung epithelial cells is a complex process involving the regulation of protein abundance of proto-oncogenes, such as c-JUN and c-MYC, by USP28. Inhibition of USP28 activity shows potential for the treatment of early-stage lung tumors and has synergistic effects with existing inhibitors targeting specific mutations.
MOLECULAR ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Thomas Fischer, Oliver Hartmann, Michaela Reissland, Cristian Prieto-Garcia, Kevin Klann, Nikolett Pahor, Christina Schuelein-Voelk, Apoorva Baluapuri, Bulent Polat, Arya Abazari, Elena Gerhard-Hartmann, Hans-Georg Kopp, Frank Essmann, Mathias Rosenfeldt, Christian Muench, Michael Flentje, Markus E. Diefenbacher
Summary: Loss of PTEN leads to increased resistance of lung cancer cells to radiation therapy, but with significant dependence on ATM kinase. Inhibition of ATM can restore sensitivity to radiation therapy and synergize with it.
CELL AND BIOSCIENCE
(2022)
Article
Cell Biology
Laura Mainz, Mohamed A. F. E. Sarhan, Sabine Roth, Ursula Sauer, Katja Maurus, Elena M. Hartmann, Helen-Desiree Seibert, Andreas Rosenwald, Markus E. Diefenbacher, Mathias T. Rosenfeldt
Summary: Autophagy is a homeostatic process that regulates the development of pancreatic ductal adenocarcinoma (PDAC). In a genetically engineered mouse model, we found that blocking autophagy reduced the incidence of PDAC, but did not affect the survival time of animals with tumors. Additionally, the absence of autophagy led to changes in the structure of the pancreas and a decrease in the number of insulin-expressing cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Nane C. Kuznik, Valeria Solozobova, Irene I. Lee, Nicole Jung, Linxiao Yang, Karin Nienhaus, Emmanuel A. Ntim, Jaice T. Rottenberg, Claudia Muhle-Goll, Amrish Rajendra Kumar, Ravindra Peravali, Simone Graessle, Victor Gourain, Celia Deville, Laura Cato, Antje Neeb, Marco Dilger, Christina A. Cramer von Clausbruch, Carsten Weiss, Bruno Kieffer, G. Ulrich Nienhaus, Myles Brown, Stefan Braese, Andrew C. B. Cato
Summary: BAG1 proteins, particularly the isoform BAG1L, play crucial roles in prostate cancer by regulating the androgen receptor (AR) and affecting gene expression related to oxidative stress and metabolism. A small molecule, A4B17, targeting the BAG domain, shows promising results in downregulating AR target genes and inhibiting prostate tumor development, outperforming the clinically approved antagonist enzalutamide. BAG1 inhibitors offer unique opportunities for antagonizing AR action and prostate cancer growth.
Article
Biochemistry & Molecular Biology
Alexandra Friesen, Susanne Fritsch-Decker, Matthias Hufnagel, Sonja Muelhopt, Dieter Stapf, Carsten Weiss, Andrea Hartwig
Summary: In vitro lung cell models have become valuable tools for toxicological risk assessment, particularly in testing advanced materials such as nanomaterials. This study investigated the cellular responses of lung cells after exposure to alpha-quartz particles using different cell culture models. The results showed dose- and time-dependent induction of genes related to inflammation and oxidative stress, with differences observed between mono-cultures and co-cultures. The findings highlight the importance of considering the cell culture model and exposure scenario in toxicological risk assessment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alexandra Friesen, Susanne Fritsch-Decker, Matthias Hufnagel, Sonja Mulhopt, Dieter Stapf, Andrea Hartwig, Carsten Weiss
Summary: Occupational exposure to particles such as crystalline quartz and its impact on the respiratory tract have been extensively studied. The development of physiologically relevant in-vitro models, specifically air-liquid interface (ALI) cell cultures, has greatly advanced the hazard assessment in recent years. This study investigated pulmonary culture models using A549 and differentiated THP-1 cells as mono- and co-cultures. The results showed the importance of considering different culture and exposure models and the relevance of communication between different cell types in toxicological investigations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Christoph Otto, Carolin Kastner, Stefanie Schmidt, Konstantin Uttinger, Apoorva Baluapuri, Sarah Denk, Mathias T. Rosenfeldt, Andreas Rosenwald, Florian Roehrig, Carsten P. Ade, Christina Schuelein-Voelk, Markus E. Diefenbacher, Christoph-Thomas Germer, Elmar Wolf, Martin Eilers, Armin Wiegering
Summary: Loss of the tumor suppressor adenomatous polyposis coli (APC) in colorectal cancer induces the upregulation of RNA polymerase I (RNAPOL1) transcription machinery, leading to increased ribosomal DNA (rDNA) transcription. Targeting RNAPOL1 with inhibitor CX5461 disrupts nucleolar integrity, induces imbalance in ribosomal proteins, and results in irreversible growth arrest with features of senescence and differentiation.
MOLECULAR ONCOLOGY
(2022)
Article
Cell Biology
Rostislav Novak, Yamen Abu Ahmad, Michael Timaner, Eliya Bitman-Lotan, Avital Oknin-Vaisman, Roi Horwitz, Oliver Hartmann, Michaela Reissland, Viktoria Buck, Mathias Rosenfeldt, David Nikomarov, Markus Elmar Diefenbacher, Yuval Shaked, Amir Orian
Summary: This study reveals that the RNF4-BMP6-RGMb axis plays a crucial role in the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) and is also involved in tumorigenesis.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Alexandra Friesen, Susanne Fritsch-Decker, Sonja Muelhopt, Caroline Quarz, Jonathan Mahl, Werner Baumann, Manuela Hauser, Manuela Wexler, Christoph Schlager, Bastian Gutmann, Tobias Krebs, Ann-Kathrin Gossmann, Frederik Weis, Matthias Hufnagel, Dieter Stapf, Andrea Hartwig, Carsten Weiss
Summary: The use of carbon fibers (CFs) has been increasing in various industries. However, a detailed toxicological evaluation of CF has not been performed yet. This study exposed human lung cell cultures to CFs and found that mechanically treated CFs induced a moderate inflammatory response, while thermo-mechanically treated CFs elicited stronger inflammatory and apoptotic effects. Thermal treatment also changed the surface properties of CFs, causing cell adhesion and subsequent loss. Triple-cultures of epithelial, macrophage, and fibroblast cells showed an exceptionally high inflammatory response. This work highlights the potentially harmful nature of CF fragments and emphasizes the need for further toxicological assessment of CF-containing materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Regina Leibe, Susanne Fritsch-Decker, Florian Gussmann, Ane Marit Wagbo, Parvesh Wadhwani, Silvia Diabate, Wolfgang Wenzel, Anne S. S. Ulrich, Carsten Weiss
Summary: For highly abundant silica nanomaterials, their detrimental effects on proteins and phospholipids are caused by hydrogen bonding, hydrophobic, and/or hydrophilic interactions. This study used biomimetic lipid vesicles with well-defined phospholipid compositions to simulate the membranolysis process induced by silica nanoparticles in human cells. The analysis demonstrated that the quaternary ammonium head groups of phosphatidylcholine and sphingomyelin play a critical and dose-dependent role in the binding and rupture of vesicles by amorphous colloidal silica nanoparticles. Surface modification with protein adsorption or covalent coupling of carboxyl groups can suppress lipid vesicle disintegration and membranolysis in human lung epithelial cells by silica nanoparticles. Molecular modeling suggests that silanol groups have a preferential affinity for choline head groups, which is influenced by the pH value. Biomimetic lipid vesicles can aid in understanding phospholipid-nanoparticle interactions at the molecular level and support the safe design of advanced materials.
Article
Cell Biology
Alexander Strubel, Philipp Muenick, Oliver Hartmann, Apirat Chaikuad, Birgit Dreier, Jonas V. Schaefer, Jakob Gebel, Christian Osterburg, Marcel Tuppi, Birgit Schaefer, Viktoria Buck, Mathias Rosenfeldt, Stefan Knapp, Andreas Pluckthun, Markus E. Diefenbacher, Volker Doetsch
Summary: p63 and p73, members of the p53 family, regulate transcriptional programs in epithelial tissues and several cell types. In cells expressing both proteins, a p63(2)/p73(2) hetero-tetramer is formed. The level of this hetero-tetramer increases in squamous cell carcinoma.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)