Article
Multidisciplinary Sciences
Fred E. Fregoso, Trevor van Eeuwen, Gleb Simanov, Grzegorz Rebowski, Malgorzata Boczkowska, Austin Zimmet, Alexis M. Gautreau, Roberto Dominguez
Summary: The Arp2/3 complex inhibitor Arpin controls cell migration by interrupting a feedback loop involving Rac-WAVE-Arp2/3 complex. This study reveals the mechanism of Arpin's inhibition through structural, biochemical, and cellular studies. The findings show that Arpin binds to Arp3, a component of the complex, similarly to nucleation-promoting factors (NPFs) that activate the complex. However, Arpin only binds to one site on Arp3, unlike NPFs that bind to two sites. Mutagenesis studies further elucidate the molecular basis for Arpin's inhibition compared to NPFs.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Chiara Brullo, Bruno Tasso
Summary: Focal adhesion kinase (Fak) is a cytoplasmic protein tyrosine kinase that is overexpressed and activated in various solid cancers, playing a significant role in metastasis, cell migration, invasion, and angiogenesis. Research is focusing on developing new, more active and selective Fak inhibitors as potential anti-cancer drugs. Currently, only a few reversible ATP-competitive inhibitors are in pre-clinical studies and clinical trials.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Jason A. Estep, Lu O. Sun, Martin M. Riccomagno
Summary: Integrin Adhesion Complexes (IACs) act as links between the cytoskeleton and extracellular environment, participating in cellular motility, tissue morphogenesis, anchorage-dependent growth, and cell survival. Focal Adhesion Kinase (FAK) is a critical organizer of IAC signaling events and a genetic and therapeutic target. This study presents the design and characterization of reversible Bimolecular Complementation sensors to monitor FAK phosphorylation in living cells, providing novel means to quantify IAC signaling.
Article
Endocrinology & Metabolism
Abigail C. Lay, Lorna J. Hale, Holly Stowell-Connolly, Robert J. P. Pope, Viji Nair, Wenjun Ju, Eva Marquez, Ruth Rollason, Jenny A. Hurcombe, Bryony Hayes, Timothy Roberts, Lawrence Gillam, Jonathan Allington, Robert G. Nelson, Matthias Kretzler, Jeff M. P. Holly, Claire M. Perks, Craig A. McArdle, Gavin I. Welsh, Richard J. M. Coward
Summary: This study identified a crucial role of IGFBP-1 in the early development of type 2 diabetic kidney disease by regulating podocyte function, suggesting that maintaining glomerular IGFBP-1 levels could be beneficial in preserving podocyte function in the early stages of DKD.
Article
Biochemistry & Molecular Biology
Amita Arora, Annukka M. Kivela, Ling Wang, Rimante Minkeviciene, Juuso H. Taskinen, Birong Zhang, Annika Koponen, Jing Sun, Michiko Shirane, You Zhou, Pirta Hotulainen, Camilla Raiborg, Vesa M. Olkkonen
Summary: During angiogenesis, Protrudin plays a key role in regulating angiogenic tube formation and promoting endothelial cell migration through its effects on FAK activation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiang Chen, Margaret Clement, M. John Hicks, Poonam Sarkar, M. Waleed Gaber, Tsz-Kwong Man
Summary: This study found that lysyl oxidase (LOX) expression was associated with cytoplasmic mislocalization of p27 in osteosarcoma. Higher LOX expression promoted cell proliferation, migration, and invasiveness, and resulted in pulmonary metastases. The upregulation of phospho-FAK was identified as the mechanism by which LOX promotes osteosarcoma metastasis. FAK inhibition may be a promising therapeutic strategy to reduce LOX-mediated metastasis in osteosarcoma.
Review
Biochemistry & Molecular Biology
Hsiang-Hao Chuang, Yen-Yi Zhen, Yu-Chen Tsai, Cheng-Hao Chuang, Michael Hsiao, Ming-Shyan Huang, Chih-Jen Yang
Summary: In this review, the authors summarize the mechanisms of FAK-mediated signaling networks during tumor development and the recent progress of FAK-targeted small-molecule compounds for anticancer activity from preclinical and clinical evidence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Qian Zhang, Mengyun Shi, Ruiling Zheng, Haoyi Han, Xin Zhang, Feng Lin
Summary: This study demonstrates that C1632 can inhibit the proliferation and migration of ovarian cancer cells by concurrently inhibiting the LIN28B/let-7/FAK signaling pathway and FAK phosphorylation, and it also exhibits a significant inhibitory effect on ovarian cancer xenograft tumors in mice. Therefore, C1632 is a promising onco-therapeutic agent for the treatment of ovarian cancer.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Review
Cell Biology
Johanne Le Coq, Ivan Acebron, Barbara Rodrigo Martin, Pilar Lopez Navajas, Daniel Lietha
Summary: In this review, the authors discuss the role of focal adhesion kinase (FAK) in cell-matrix adhesion and migration. They highlight the known structural features of FAK and the challenges in understanding its integration in the focal adhesion complex and the structural changes during focal adhesion maturation.
JOURNAL OF CELL SCIENCE
(2022)
Article
Microbiology
David M. Hershey, Aretha Fiebig, Sean Crosson
Summary: In this study, genes required for Caulobacter crescentus to activate surface attachment in response to signals from flagellum were identified. The genes involved in transmitting information from the flagellum are grouped into two pathways, one controlling the bacterium's morphogenesis program and the other required for flagellar motility. The results support a model where a developmental and a mechanical signaling pathway operate in parallel downstream of the flagellum and converge to regulate adhesion.
Article
Medicine, Research & Experimental
Dezhong Zheng, Mingyu Zhang, Tingrong Liu, Tao Zhou, Anna Shen
Summary: The study reveals that OPG induces cardiomyocyte hypertrophy and inhibits autophagy through the FAK/Beclin1 signal pathway. The phosphorylation of FAK plays a key role in this process.
Review
Oncology
John C. Dawson, Alan Serrels, Dwayne G. Stupack, David D. Schlaepfer, Margaret C. Frame
Summary: Focal adhesion kinase (FAK) is commonly overexpressed in cancer and involved in various oncogenic processes and resistance mechanisms, making FAK inhibitors most effective as combination therapies in selected patient populations.
NATURE REVIEWS CANCER
(2021)
Review
Biochemistry & Molecular Biology
Andrea Spallarossa, Bruno Tasso, Eleonora Russo, Carla Villa, Chiara Brullo
Summary: This review focuses on compounds that can block Focal adhesion kinase (FAK) with different potencies and mechanisms of action, as well as the emergence of new PROTAC molecules. It provides important insights into new FAK inhibitors and offers information for future investigations, particularly from a medicinal chemistry perspective.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Xu Chen, Zheng-Qian Guo, Dan Cao, Yong Chen, Jian Chen
Summary: The study revealed that overexpression of PNO1 in glioma is associated with poor prognosis, and that PNO1 contributes to glioma progression by activating THBS1/FAK/Akt signaling pathway.
CELL DEATH & DISEASE
(2021)
Article
Physics, Fluids & Plasmas
C. Alex Safsten, Volodmyr Rybalko, Leonid Berlyand
Summary: This study focuses on the onset of motion of a living cell driven by myosin contraction. The results show that stable asymmetric moving states bifurcate from unstable radial stationary states, and these moving states have nonlinear asymptotic stability in modeling observable steady cell motion.
Article
Biochemistry & Molecular Biology
Stefan Koch, Sergio P. Acebron, Jessica Herbst, Gencay Hatiboglu, Christof Niehrs
Article
Developmental Biology
Leila R. Martins, Raffaela K. Bung, Stefan Koch, Karsten Richter, Laura Schwarzmueller, Dorothee Terhardt, Bahtiyar Kurtulmus, Christof Niehrs, Arefeh Rouhi, Ingrid Lohmann, Gislene Pereira, Stefan Froehling, Hanno Glimm, Claudia Scholl
DEVELOPMENTAL BIOLOGY
(2018)
Article
Pathology
Philipp-Alexander Neumann, Stefan Koch, Roland S. Hilgarth, Ernesto Perez-Chanona, Patricia Denning, Christian Jobin, Asma Nusrat
AMERICAN JOURNAL OF PATHOLOGY
(2014)
Article
Immunology
Manirath Khounlotham, Wooki Kim, Eric Peatman, Porfirio Nava, Oscar Medina-Contreras, Caroline Addis, Stefan Koch, Benedicte Fournier, Asma Nusrat, Timothy L. Denning, Charles A. Parkos
Meeting Abstract
Gastroenterology & Hepatology
Porfirio Nava, Ryuta Kamekura, Oscar Medina-Contreras, Ross Hamilton, Keli Kolegraff, Stefan Koch, Timothy Denning, Charkes Parkos, Asma Nusrat
INFLAMMATORY BOWEL DISEASES
(2012)
Review
Cell Biology
Lavanya Moparthi, Stefan Koch
Article
Multidisciplinary Sciences
Lavanya Moparthi, Giulia Pizzolato, Stefan Koch
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Gastroenterology & Hepatology
Celia Escudero-Hernandez, Andreas Munch, Ann-Elisabet Ostvik, Atle van Beelen Granlund, Stefan Koch
JOURNAL OF CROHNS & COLITIS
(2020)
Article
Cell Biology
Lavanya Moparthi, Stefan Koch
Review
Oncology
Stefan Koch
Summary: This article discusses the control of the Wnt pathway by FOX proteins, and the contribution of their interaction to cancer initiation and progression. Further research on FOX biology may lead to new targeted treatments for cancer.
Article
Cell Biology
Andrea Molinas, Stephanie Heil, Stefan Koch
Summary: While the CCNY gene has been implicated in the pathogenesis of inflammatory bowel disease, a study investigating the role of cyclin Y in intestinal epithelial cells found that its absence did not significantly affect cell proliferation or disease activity. The low expression of cyclin-Y-associated CDKs in IECs suggests that cyclin Y may not play a crucial role in intestinal epithelial homeostasis, casting doubt on CCNY mutations as causative factors in IBD.
Article
Cell Biology
Giulia Pizzolato, Lavanya Moparthi, Simon Soderholm, Claudio Cantu, Stefan Koch
Summary: The forkhead box transcription factor FOXQ1 plays a crucial role in the pathogenesis of colorectal cancers by promoting tumour metastasis and activating the Wnt/beta-catenin signalling pathway. FOXQ1 enhances Wnt-induced transcription and regulates the expression of beta-catenin target genes. It also controls the expression of various Wnt target genes independently of beta-catenin. Furthermore, FOXQ1 is involved in a transcriptional program linked to EMT and cell migration, and interacts with similar co-factors as the beta-catenin-associated transcription factor Tcf711.
JOURNAL OF CELL SCIENCE
(2022)
Review
Cell Biology
Stefan Koch
Article
Cell Biology
Nadine Kirsch, Ling-Shih Chang, Stefan Koch, Andrey Glinka, Christine Dolde, Gabriele Colozza, Maria D. J. Benitez, Edward M. De Robertis, Christof Niehrs
DEVELOPMENTAL CELL
(2017)
Article
Biochemistry & Molecular Biology
Birgit S. Berger, Sergio P. Acebron, Jessica Herbst, Stefan Koch, Christof Niehrs
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
