Article
Biochemistry & Molecular Biology
Agata Gluszek-Kustusz, Benjamin Craske, Thibault Legal, Toni McHugh, Julie P. Welburn
Summary: During mitosis, the microtubule crosslinker protein PRC1 recruits motor proteins CENP-E and Kif4A to the central spindle to coordinate chromosome segregation and cytokinesis. This recruitment is mediated by a conserved hydrophobic motif and is spatially and temporally regulated by CENP-E. The interaction between PRC1 and microtubule motor proteins is essential for controlling chromosome partitioning, maintaining central spindle integrity, and ensuring cytokinesis during anaphase.
Article
Cell Biology
Zhe Han, Xin Hao, Cheng-Jie Zhou, Jun Wang, Xin Wen, Xing-Yue Wang, De-Jian Zhang, Cheng-Guang Liang
Summary: The study investigated the expression profiles and functional roles of CLTC in sheep oocytes and found that CLTC plays crucial regulatory roles in sheep oocyte maturation and early embryo development, especially in spindle dynamics and early embryo development. The results indicate that CLTC is essential for spindle formation and chromosomal alignment, as well as for proper early embryo development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Kruno Vukusic, Iva M. Tolic
Summary: This review discusses the significance of chromosome congression and alignment in mitosis, as well as the factors that can lead to misalignment and missegregation. It also highlights the protective mechanisms employed by cells to prevent erroneous kinetochore-microtubule interactions and aneuploidy.
Article
Cell Biology
Eric M. Kramer, P. A. Tayjasanant, Bethan Cordone
Summary: During mitosis in higher eukaryotes, chromosome condensation is co-regulated by several gene families, with chromosome size and DNA content scaling with powers close to 1/4, 1/2, and 1 across vertebrate species. The increase in chromosome cross-sectional area with length is hypothesized to be necessary to prevent large chromosome elongations that could slow or stall mitosis. Unlike angiosperms, individual vertebrate karyotypes typically exhibit a wider range of chromosome lengths.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Yuqing Zhang, Chunlin Song, Lei Wang, Hongfei Jiang, Yujing Zhai, Ying Wang, Jing Fang, Gang Zhang
Summary: This review summarizes the research progress on the role of Bub1 in mitosis over the past three decades. The studies have revealed that Bub1 plays important roles in checkpoint regulation and chromosome alignment. The essentiality of Bub1 in higher eukaryotic cells is discussed, along with some unresolved questions raised for future study.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Saara Hamalisto, Jonathan Stahl-Meyer, Marja Jaaettela
Summary: Mitosis, a tightly regulated process in mammalian life, can still result in errors leading to chromosomal instabilities and malignancies. Recent discoveries have shown unexpected roles for autophagy and lysosomes in preserving genomic integrity during mitosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Pablo Lara-Gonzalez, Jonathon Pines, Arshad Desai
Summary: The mitotic spindle is a macromolecular machine responsible for faithful chromosome segregation during cell division. Assembly of the spindle is guided by the 'Search & Capture' principle in structured space. Nucleation of noncentrosomal microtubules near kinetochores and changes in kinetochore architecture promote rapid and accurate assembly of the spindle.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Review
Oncology
Lucia Paolini, Sajjad Hussain, Paul J. J. Galardy
Summary: Genomic instability is a central factor in the pathogenesis of human cancer. Most of the time, chromosome instability negatively affects cellular fitness, but in cancer it is usually associated with worse prognosis. Neuroblastoma, a common solid tumor in children, has two distinct patterns of genomic instability, with whole-chromosome aneuploidy associated with better prognosis.
FRONTIERS IN ONCOLOGY
(2022)
Review
Cell Biology
Alexandra P. Navarro, Iain M. Cheeseman
Summary: Faithful chromosome segregation in metazoans requires the establishment of a metaphase plate where all chromosomes are attached to mitotic spindle microtubules and aligned at the cell equator. This process relies on the precise coordination between multiple mechanisms that start upon nuclear envelope breakdown and culminate with chromosome congression to the spindle equator during prometaphase. Error correction mechanisms are in place to sense and correct erroneous kinetochore-microtubule attachments before anaphase.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Susana Eibes, Girish Rajendraprasad, Claudia Guasch-Boldu, Mirela Kubat, Yulia Steblyanko, Marin Barisic
Summary: It is unknown how the kinetochore fibrous corona is disassembled. Here, the authors reveal that Aurora A and B kinases-mediated phosphorylation activates CENP-E, which is essential to prevent the premature removal of corona proteins by dynein.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Ana C. Almeida, Joana Soares-de-Oliveira, Danica Drpic, Liam P. Cheeseman, Joana Damas, Harris A. Lewin, Denis M. Larkin, Paulo Aguiar, Antonio J. Pereira, Helder Maiato
Summary: Chromosome segregation in mammals relies on the maturation of K-fibers, and augmin plays a key role in this process. Our study reveals that augmin promotes turnover and poleward flux of kinetochore and interpolar microtubules and increases the frequency of microtubule growth through branched microtubule nucleation. These findings are important for understanding the self-organization and maturation of K-fibers in mammals.
Article
Cell Biology
Rui Lyu, Xuemei Wu, Nan Ma, Difei Wang, Shuang Sun, Youguang Luo, Jun Zhou, Xinyi Lu, Min Liu, Dengwen Li
Summary: Human embryonic stem cells exhibit specialized mitotic behavior characterized by unique spindle morphology, increased astral microtubules, stable spindle microtubules, and faster chromosome alignment compared to somatic cells. The spindle assembly checkpoint is functional in hESCs, highlighting potential implications for their proliferation and differentiation processes.
CELL AND TISSUE RESEARCH
(2022)
Article
Cell Biology
Aaron R. Tipton, Gary J. Gorbsky
Summary: This study disrupts kinetochores and reveals that in the absence of functional kinetochores, microtubule dynamics still exhibit slow and fast turnover populations. Additionally, it suggests that other subpopulations contribute to the slow turnover population.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biology
Yuting Liu, Kehui Wang, Li Huang, Jicheng Zhao, Xinpeng Chen, Qiang Wu, Zhouliang Yu, Guohong Li
Summary: The phosphorylation of CENP-A Ser68 is crucial for the cell-cycle-dependent deposition of CENP-A and cell viability.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Marin Barisic, Girish Rajendraprasad
Summary: Recent research has shown that in human cells, the continuous poleward motion of microtubules in mitotic spindles is driven by four mitotic kinesins, with specific contributions at different stages of mitosis. While the molecular mechanism behind this cellular phenomenon has been elucidated, the functional roles of microtubule poleward flux during cell division remain largely unknown.
Article
Biochemical Research Methods
Sunkyu Choi, Jonathan Kelber, Xinning Jiang, Jan Strnadel, Ken Fujimura, Martina Pasillas, Judith Coppinger, Richard Klemke
ANALYTICAL BIOCHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Chang-Young Jang, Judith A. Coppinger, John R. Yates, Guowei Fang
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2011)
Article
Biochemistry & Molecular Biology
Laurie S. Minamide, Sankar Maiti, Judith A. Boyle, Richard C. Davis, Judith A. Coppinger, Yunhe Bao, Timothy Y. Huang, John Yates, Gary M. Bokoch, James R. Bamburg
JOURNAL OF BIOLOGICAL CHEMISTRY
(2010)
Article
Cell Biology
Chang-Young Jang, Judith A. Coppinger, Akiko Seki, John R. Yates, Guowei Fang
JOURNAL OF CELL SCIENCE
(2009)
Article
Biochemical Research Methods
Martina P. Pasillas, Sarah Shields, Rebecca Reilly, Jan Strnadel, Christian Behl, Robin Park, John R. Yates, Richard Klemke, Steven L. Gonias, Judith A. Coppinger
MOLECULAR & CELLULAR PROTEOMICS
(2015)
Article
Biochemistry & Molecular Biology
Guang-Hui Liu, Tinglu Guan, Kaustuv Datta, Judith Coppinger, John Yates, Larry Gerace
MOLECULAR AND CELLULAR BIOLOGY
(2009)
Article
Cell Biology
Atanas V. Koulov, Paul LaPointe, Bingwen Lu, Abbas Razvi, Judith Coppinger, Meng-Qiu Dong, Jeanne Matteson, Rob Laister, Cheryl Arrowsmith, John R. Yates, William E. Balch
MOLECULAR BIOLOGY OF THE CELL
(2010)
Article
Neurosciences
Wei-Chieh Chiang, Heike Kroeger, Sanae Sakami, Carissa Messah, Douglas Yasumura, Michael T. Matthes, Judith A. Coppinger, Krzysztof Palczewski, Matthew M. LaVail, Jonathan H. Lin
MOLECULAR NEUROBIOLOGY
(2015)
Article
Multidisciplinary Sciences
Judith A. Coppinger, Darren M. Hutt, Abbas Razvi, Atanas V. Koulov, Sandra Pankow, John R. Yates, William E. Balch
Article
Multidisciplinary Sciences
Patricia M. Gaffney, Denise M. Imai, Deana L. Clifford, Majid Ghassemian, Roman Sasik, Aaron N. Chang, Timothy D. O'Brien, Judith Coppinger, Margarita Trejo, Eliezer Masliah, Linda Munson, Christina Sigurdson
Article
Multidisciplinary Sciences
Wei-meng Zhao, Judith A. Coppinger, Akiko Seki, Xiao-li Cheng, John R. Yates, Guowei Fang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2008)
Article
Multidisciplinary Sciences
Akiko Seki, Judith A. Coppinger, Chang-Young Jang, John R. Yates, Guowei Fang
Article
Multidisciplinary Sciences
R. Reilly, M. S. Mroz, E. Dempsey, K. Wynne, S. J. Keely, E. F. McKone, C. Hiebel, C. Behl, J. A. Coppinger
SCIENTIFIC REPORTS
(2017)
Article
Respiratory System
Zivile Useckaite, Mark P. Ward, Anne Trappe, Rebecca Reilly, Jenny Lennon, Holly Davage, David Matallanas, Hilary Cassidy, Eugene T. Dillon, Kiva Brennan, Sarah L. Doyle, Suzanne Carter, Seamas Donnelly, Barry Linnane, Edward F. McKone, Paul McNally, Judith A. Coppinger
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)