Article
Biochemistry & Molecular Biology
Angel Oliveras, Luis Moll, Gerard Riesco-Llach, Arnau Tolosa-Canudas, Sergio Gil-Caballero, Esther Badosa, Anna Bonaterra, Emilio Montesinos, Marta Planas, Lidia Feliu
Summary: In this study, lipopeptides derived from BP100 were modified to improve their biological profile, with analogues containing D-amino acids at position 4 showing promising results against plant pathogenic bacteria and fungi. Among these modified lipopeptides, BP475 and BP485, incorporating D-Phe at position 4 and a butanoyl group, demonstrated the best antimicrobial activity with low hemolysis and phytotoxicity. NMR characterization revealed that the secondary structure of BP475 plays a key role in its antimicrobial activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Sara Khosrow Shahi, Zohreh Didar, Mohammad Ali Hesarinejad, Mohsen Vazifedoost
Summary: The optimization of pulsed electric field parameters for extracting natural saponins from Chubak root had significant effects on the properties of the samples. The Chubak root extract obtained under the optimal conditions showed good performance in ice cream formulation.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Chemistry, Physical
M. Dabestani, S. Yeganehzad, R. Miller
Summary: Chubak, a shrubby plant with saponin-rich roots, has potential as a natural surfactant source. Research on the surface activity of Chubak root extract and purified saponin showed a consistent trend, but the purification method was found to be inefficient in separating the complete saponin content.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Food Science & Technology
Sina Aryan, Amir M. Mortazavian, Fatemeh Mohammadi, Vahideh Mahdavi, Narges Moazami, Sahar Jazaeri
Summary: The Aqueous Extract of Acanthophyllum laxiusculum root exhibits excellent foaming and antioxidant properties in the food industry, with spray drying as a recommended method for convenient application. The addition of the root extract to malt extract improves foaming and antioxidant capabilities, suitable for inhibiting yeast growth.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Biochemistry & Molecular Biology
Boyana Paarvanova, Bilyana Tacheva, Gergana Savova, Miroslav Karabaliev, Radostina Georgieva
Summary: Saponins are organic amphiphilic substances mainly extracted from herbs, with various biological activities. This study focused on the mechanism of saponins' effects on erythrocytes, revealing that saponins induce hemolysis through colloid-osmotic pore formation in the membrane.
Article
Immunology
Hyunjung Kim, Jigui Yu, Di Bai, Moon H. H. Nahm, Pengfei Wang
Summary: Both VSA-1 and QS-21 can enhance immune responses, particularly beneficial for breakthroughs related to PCV13. Due to its accessibility and lower toxicity, VSA-1 can be included in a new vaccine formulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biophysics
Da Wang, Luping Sha, Chen Xu, Ying Huang, Chengcheng Tang, Tingting Xu, Xianzhe Li, Donghua Di, Jie Liu, Li Yang
Summary: Through bottom-up technique, two saponin-cholesterol nano-complexes were designed and prepared, showing potent antitumor activity in vitro and in vivo. Compared to the free drug, these nano complexes have lower toxicity and reduced hemolysis.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Lisa G. M. Huis In 't Veld, Nataschja Ho, Melisssa Wassink, Martijn H. den Brok, Gosse J. Adema
Summary: Saponin-based adjuvants (SBAs) are a promising new type of adjuvant that can enhance dendritic cell-mediated antigen cross-presentation and activate CD8 + T cells by inducing lipid bodies. The study reveals that SBAs specifically induce the PERK pathway and depend on its activation for dendritic cell cross-presentation. Additionally, the MHCII(lo)CD11b(hi) subset of dendritic cells shows the highest responsiveness to SBAs.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Immunology
Sabrina de Oliveira Emerick, Thais Vieira de Carvalho, Bianca Meirelles Miranda, Adriana Carneiro da Silva, Thais Viana Fialho Martins, Leandro Licursi de Oliveira, Eduardo de Almeida Marques-da-Silva
Summary: The study evaluated the immunization using different vaccines and adjuvants against L. chagasi infection, and found that the LPG3-SAP vaccine significantly reduced parasite load in both spleen and liver in mice, inducing Th1/Th17 response and IL-10 production.
Article
Immunology
Teerasit Techawiwattanaboon, Thomas Courant, Livia Brunner, Suwitra Sathean-anan-kun, Pratomporn Krangvichian, Nutta Iadsee, Yaowarin Nakornpakdee, Noppadon Sangjun, Pat Komanee, Nicolas Collin, Kiat Ruxrungtham, Kanitha Patarakul
Summary: This study evaluated the potential of four adjuvants in leptospirosis vaccines and found that LQ, LQuil, and SQuil adjuvants performed similarly to the LMQ adjuvant in enhancing immune protection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Yuxuan Huang, Jinzhe Li, Yue Liu, Munkh-Amgalan Gantumur, Narantuya Sukhbaatar, Panpan Zhao, Kwang Chol Oh, Zhanmei Jiang, Juncai Hou
Summary: The binding interactions, gas-water interface properties, and bioactivities between alpha-lactalbumin (alpha La) and tea saponin (Ts), gynostemma saponin (Gyp), and tribulus saponin (Tr) were characterized. The results showed that alpha La interacted with Ts and Gyp through hydrogen bond and van der Waals force, while it combined with Tr via hydrophobic interaction force. Ts, Gyp, and Tr increased the foaming ability of alpha La. The DPPH scavenging ability, ferrous reducing power, and alpha-glucosidase inhibition of alpha La-saponins were higher than those of alpha La, with alpha La-Ts showing the highest activity. The study provides valuable insights into the interaction mechanism between natural saponins and proteins for developing protein-saponin foaming agents in the food industry.
FOOD HYDROCOLLOIDS
(2023)
Article
Immunology
Xing Luo, Zhen Song, Xiaogqiang Zeng, Yan Ye, Hailin Zheng, Dingyi Cai, Qingpeng Yuan, Haibo Li, Yanan Tong, Dongshui Lu, Yuheng Liu, Hao Zeng, Yun Yang, Hongwu Sun, Quanming Zou
Summary: A novel anti-tumor vaccine composed of a plant-derived immunostimulant molecular nanoadjuvant (SND) and the antigen OVA was designed to reinvigorate the immune response and inhibit tumor progression. The results showed that this novel nanoadjuvant had good stability and low toxicity, and could significantly improve the humoral and cellular immune response, leading to preventive and therapeutic effects against tumors.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemical Research Methods
Ana M. Simonet, Alexandra G. Duran, Andy J. Perez, Francisco A. Macias
Summary: This study reviewed the C-13 and H-1 NMR spectroscopic data of aglycones from Agave saponins reported in the literature and developed a convenient identification method for these natural products.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Parasitology
Marcelo M. Jesus, Daniela P. Lage, Danniele L. Vale, Camila S. Freitas, Breno L. Pimenta, Gabriel J. L. Moreira, Fernanda F. Ramos, Isabela A. G. Pereira, Raquel S. Bandeira, Fernanda Ludolf, Grasiele S. V. Tavares, Alexsandro S. Galdino, Mariana C. Duarte, Daniel Menezes-Souza, Miguel A. Chavez-Fumagalli, Antonio L. Teixeira, Denise U. Goncalves, Bruno M. Roatt, Myron Christodoulides, Vivian T. Martins, Eduardo A. F. Coelho
Summary: Tegumentary leishmaniasis is the main clinical manifestation of leishmaniasis. The development of prophylactic vaccines could be considered as a strategy to control the disease. The study showed that the LiHyp1 protein plus adjuvant was protective against tegumentary leishmaniasis and could be considered as a vaccine candidate against human disease.
PARASITOLOGY RESEARCH
(2023)
Article
Plant Sciences
Yixuan Sui, Xiaoyue Huang, Mallique Qader, Pengtao You, Guoping Gan, Xueling Xi, Jianchao Zhang, Shugeng Cao
Summary: Two new oleanane-type triterpene glycosides and two known compounds were isolated from the rhizome of Impatiens pritzellii var. hupehensis. The structures were determined through NMR and HRESIMS analysis, and the T-lymphocyte proliferation activity of the isolated terpenoid saponins was evaluated using the CCK-8 colorimetric method.
PHYTOCHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Egor S. Zhilin, Nadezhda E. Ustyuzhanina, Leonid L. Fershtat, Nikolay E. Nifantiev, Nina N. Makhova
Summary: The newly synthesized (1,2,5-oxadiazolyl)azasydnones showed excellent antiplatelet activity and selective inhibition of platelet aggregation mediated by ADP and adrenaline, while being completely non-toxic to the hybrid endothelial cells EaHy 926. This makes them promising drug candidates with improved antiplatelet profile and reduced toxicity for potential use in medicinal chemistry and drug design.
CHEMICAL BIOLOGY & DRUG DESIGN
(2022)
Article
Biochemistry & Molecular Biology
Olga N. Yudina, Marina L. Gening, Pinaki Talukdar, Alexey G. Gerbst, Yury E. Tsvetkov, Nikolay E. Nifantiev
Summary: This paper describes the synthesis of a linear tetramer and subsequent formation of a cyclic tetrasaccharide with axial amino groups starting from 3-azido-3-deoxy-1,2:5,6-di-O-isopropylidene-alpha-D-allofuranose. This cyclic tetramer serves as a potential scaffold for the synthesis of tetravalent functional clusters.
CARBOHYDRATE RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Mikhail Kiselevskiy, Natalia Yu Anisimova, Nadezhda E. Ustyuzhanina, Dmitry Z. Vinnitskiy, Alexandra Tokatly, Vera V. Reshetnikova, Irina O. Chikileva, Irina Zh Shubina, Kirill Kirgizov, Nikolay E. Nifantiev
Summary: Fucoidans, natural sulfated polysaccharides, have potential antitumor and anti-inflammatory properties. Although current experiments have shown low antitumor activity, there is potential for their application in supportive care and symptomatic therapy for cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Danila V. Reshetnikov, Igor D. Ivanov, Dmitry S. Baev, Tatyana V. Rybalova, Evgenii S. Mozhaitsev, Sergey S. Patrushev, Valentin A. Vavilin, Tatyana G. Tolstikova, Elvira E. Shults
Summary: Xanthine derivatives are widely used for the development of effective bioactive agents. In this study, 38 methylxanthine derivatives were designed and synthesized as acetylcholinesterase inhibitors. Different reaction methods were employed to synthesize substituted methylxanthine derivatives. The bioactivity of the compounds was evaluated, and the structure-activity relationships were discussed. Compounds 53, 59, 65, 66, and 69 exhibited the strongest inhibitory activity against AChE.
Article
Biochemistry & Molecular Biology
Dmitry S. Baev, Mikhail E. Blokhin, Varvara Yu. Chirkova, Svetlana V. Belenkaya, Olga A. Luzina, Olga I. Yarovaya, Nariman F. Salakhutdinov, Dmitry N. Shcherbakov
Summary: Although the incidence and mortality of SARS-CoV-2 infection has been declining during the pandemic, the search for novel antiviral drugs remains relevant. In this study, triterpenic acid amides were found to inhibit the SARS-CoV-2 main protease through non-covalent interactions. Compounds 1e and 1b demonstrated inhibitory effects at micromolar concentrations.
Correction
Biotechnology & Applied Microbiology
Vasilina Farofonova, Nadeshda Andreeva, Ekaterina Kulakovskaya, Azamat Karginov, Michael Agaphonov, Tatiana Kulakovskaya
FOLIA MICROBIOLOGICA
(2023)
Article
Biotechnology & Applied Microbiology
Vasilina Farofonova, Nadeshda Andreeva, Ekaterina Kulakovskaya, Azamat Karaginov, Michael Agaphonov, Tatiana Kulakovskaya
Summary: In this study, the pleiotropic effects of PHO91 gene knockout were demonstrated in methylotrophic yeast. The knockout led to higher levels of acid-soluble and acid-insoluble inorganic polyphosphate, decreased resistance to cadmium, and increased resistance to manganese and peroxide stresses. The mutant strain was also unable to consume methanol.
FOLIA MICROBIOLOGICA
(2023)
Article
Chemistry, Multidisciplinary
Sergey A. Popov, Zhiwen Qi, Chengzhang Wang, Elvira E. Shults
Summary: Three compounds containing isothiocyanate groups at different positions from the C-17 of ursolic acid have been synthesized through the modification of ursolic acid-derived primary amines. By reacting amines and ammonia with novel isothiocyanates, terpene hybrids with thioureas and N,S-containing heterocycles were obtained. The reactions with propargylamine yielded triterpenoid hybrids with 4,5-dihydrothiazol-2-yl-amine, while the reactions with (1-aryl-1H-1,2,3-triazole-4-yl)methanamines and ammonia resulted in thiourea derivatives.
JOURNAL OF SULFUR CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Maria I. Bilan, Natalia Yu. Anisimova, Alexandra I. Tokatly, Sofya P. Nikogosova, Dmitriy Z. Vinnitskiy, Nadezhda E. Ustyuzhanina, Andrey S. Dmitrenok, Evgenia A. Tsvetkova, Mikhail V. Kiselevskiy, Nikolay E. Nifantiev, Anatolii I. Usov
Summary: Crude anionic polysaccharides from Lethasterias fusca were purified and analyzed. The main fraction LF was identified as having a dermatan core structure and the major component as dermatan sulfate LF-Derm. Unusual sulfation patterns were observed in the polysaccharides. LF-Derm and LF-Hep were found to stimulate hematopoiesis in vitro, suggesting that high sulfation may not be necessary for hematopoiesis stimulation in this case.
Article
Biochemistry & Molecular Biology
Igor A. Schepetkin, Zhangeldy S. Nurmaganbetov, Serik D. Fazylov, Oralgazy A. Nurkenov, Andrei I. Khlebnikov, Tulegen M. Seilkhanov, Anarkul S. Kishkentaeva, Elvira E. Shults, Mark T. Quinn
Summary: Alzheimer's disease is a common neurodegenerative disease characterized by memory loss and cognitive impairment. The loss of cholinergic neurons is one of the main causes of this disease. Current acetylcholinesterase inhibitors have limitations, so there is ongoing research to find more effective inhibitors. In this study, new lupinine triazole derivatives were synthesized and evaluated for their inhibitory activity. The most promising compound showed mixed-type inhibition and molecular docking studies revealed the interaction between the compound and the target enzyme. A structure-activity relationship model was also developed to guide the design of more potent inhibitors.
Article
Biochemistry & Molecular Biology
Kirill P. P. Cheremnykh, Arkadiy O. O. Bryzgalov, Dmitry S. S. Baev, Sergey A. A. Borisov, Yulia S. S. Sotnikova, Victor A. A. Savelyev, Tatyana G. G. Tolstikova, Shamansur S. S. Sagdullaev, Elvira E. E. Shults
Summary: Diterpenoid alkaloids derived from natural tetracyclic diterpenes have attracted scientists' interest due to their medicinal uses and toxic side effects. A study focused on the alkaloid lappaconitine extracted from Aconitum and Delphinium species and proposed a method to construct hybrid molecules containing lappaconitine and benzodiazepine fragments. The newly synthesized compounds showed low toxicity and excellent analgesic activity, as well as antiarrhythmic effects, providing potential for future modifications of lappaconitine to reduce side effects while maintaining therapeutic efficacy.
Article
Biochemistry & Molecular Biology
Anastasia A. A. Kamneva, Dmitry V. V. Yashunsky, Elena A. A. Khatuntseva, Nikolay E. E. Nifantiev
Summary: Spacer-armed pseudodi-, pseudotetra-, and pseudohexasaccharides related to the capsular phosphoglycan of Haemophilus influenzae type a were synthesized using iterative chain elongation. These compounds were used for designing neoglycoconjugates, such as a biotinylated glycoconjugate suitable for immunological studies.
Article
Biochemistry & Molecular Biology
Irina O. Chikileva, Alexandra V. Bruter, Nadezhda A. Persiyantseva, Maria A. Zamkova, Raimonda Ya. Vlasenko, Yuliya I. Dolzhikova, Irina Zh. Shubina, Fedor V. Donenko, Olga V. Lebedinskaya, Darina V. Sokolova, Vadim S. Pokrovsky, Polina O. Fedorova, Nadezhda E. Ustyuzhanina, Natalia Yu. Anisimova, Nikolay E. Nifantiev, Mikhail V. Kiselevskiy
Summary: This study aimed to develop a safer HER2-targeting CAR therapy by delivering CAR constructs into T and NK cells using a non-viral transfection method. The generated CAR-T-NK cells showed potent anti-tumor activity, suggesting transient expression of anti-HER2 CARs as a safer treatment for HER2-positive human cancers.
Article
Chemistry, Organic
Arsenii S. Solovev, Evgeniya M. Denisova, Ekaterina A. Kurbatova, Olga Y. Kutsevalova, Liubov G. Boronina, Vladimir A. Ageevets, Sergey V. Sidorenko, Vadim B. Krylov, Nikolay E. Nifantiev
Summary: Methylphosphorylated oligosaccharides structurally related to the O-antigens of Klebsiella pneumoniae were synthesized and used for antibody detection in ELISA. The antibodies specifically recognized the synthesized oligosaccharides, suggesting their potential use for serological diagnostics and strain serotyping of Klebsiella pneumoniae.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
