Minimalist Approaches to Protein Labelling: Getting the Most Fluorescent Bang for Your Steric Buck

Significant Expansion of the Fluorescent Protein Chromophore through the Genetic Incorporation of a Metal-Chelating Unnatural Amino Acid

(2013) Xiaohong Liu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein conjugation with genetically encoded unnatural amino acids

(2013) Chan Hyuk Kim et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and Förster Resonance Energy Transfer/Luminescence Resonance Energy Transfer Studies

(2013) Lee C. Speight et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Thioamide Quenching of Fluorescent Probes through Photoinduced Electron Transfer: Mechanistic Studies and Applications

(2013) Jacob M. Goldberg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Labeling Proteins with Fluorophore/Thioamide Förster Resonant Energy Transfer Pairs by Combining Unnatural Amino Acid Mutagenesis and Native Chemical Ligation

(2013) Rebecca F. Wissner et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Genetically Encoded Fluorescent Probe in Mammalian Cells

(2013) Abhishek Chatterjee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Dynamics of internal pore opening in KV channels probed by a fluorescent unnatural amino acid

(2013) T. Kalstrup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expanding the Genetic Code of Caenorhabditis elegans Using Bacterial Aminoacyl-tRNA Synthetase/tRNA Pairs

(2012) Angela R. Parrish et al.   ACS Chemical Biology

Multiple-Site Labeling of Proteins with Unnatural Amino Acids

(2012) Karin V. Loscha et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites

(2012) Kiyoshi Ozawa et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The Extent of Pyrene Excimer Fluorescence Emission Is a Reflector of Distance and Flexibility: Analysis of the Segment Linking the LDL Receptor-Binding and Tetramerization Domains of Apolipoprotein E3

(2012) Gursharan K. Bains et al.   BIOCHEMISTRY

Two-Color Fluorescent l-Amino Acid Mimic of Tryptophan for Probing Peptide–Nucleic Acid Complexes

(2012) Aleksandr V. Strizhak et al.   BIOCONJUGATE CHEMISTRY

Painting proteins blue: β-(1-azulenyl)-l-alanine as a probe for studying protein–protein interactions

(2012) Yurii S. Moroz et al.   CHEMICAL COMMUNICATIONS

Interrogating Signaling Nodes Involved in Cellular Transformations Using Kinase Activity Probes

(2012) Cliff I. Stains et al.   CHEMISTRY & BIOLOGY

Quantification of Local Hydration at the Surface of Biomolecules Using Dual-Fluorescence Labels

(2012) Vasyl G. Pivovarenko et al.   JOURNAL OF PHYSICAL CHEMISTRY A

Minimalist Probes for Studying Protein Dynamics: Thioamide Quenching of Selectively Excitable Fluorescent Amino Acids

(2012) Jacob M. Goldberg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Native Chemical Ligation of Thioamide-Containing Peptides: Development and Application to the Synthesis of Labeled α-Synuclein for Misfolding Studies

(2012) Solongo Batjargal et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Photophysical Properties of 7-(diethylamino)Coumarin-3-carboxylic Acid in the Nanocage of Cyclodextrins and in Different Solvents and Solvent Mixtures

(2012) Aninda Chatterjee et al.   PHOTOCHEMISTRY AND PHOTOBIOLOGY

A Fluorescent Reporter of the Phosphorylation Status of the Substrate Protein STAT3

(2011) Vanessa K. Lacey et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Subcellular Protein Localization by Using a Genetically Encoded Fluorescent Amino Acid

(2011) Godefroid Charbon et al.   CHEMBIOCHEM

Thioamide quenching of intrinsic protein fluorescence

(2011) Jacob M. Goldberg et al.   CHEMICAL COMMUNICATIONS

Imaging Protein–Protein Interactions inside Living Cells via Interaction-Dependent Fluorophore Ligation

(2011) Sarah A. Slavoff et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-Specific Fluorescent Labeling of Nascent Proteins on the Translating Ribosome

(2011) Ishu Saraogi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Amyloid-β forms fibrils by nucleated conformational conversion of oligomers

(2011) Jiyong Lee et al.   Nature Chemical Biology

Genetically Encoding Unnatural Amino Acids in Neural Stem Cells and Optically Reporting Voltage-Sensitive Domain Changes in Differentiated Neurons

(2011) Bin Shen et al.   STEM CELLS

A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein inEscherichia coli

(2010) Wei Wan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Modulation ofp-Cyanophenylalanine Fluorescence by Amino Acid Side Chains and Rational Design of Fluorescence Probes of α-Helix Formation

(2010) Humeyra Taskent-Sezgin et al.   BIOCHEMISTRY

Blue Fluorescent Amino Acids as In Vivo Building Blocks for Proteins

(2010) Lars Merkel et al.   CHEMBIOCHEM

Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot–peptide complex

(2010) Youngseon Choi et al.   CHEMICAL COMMUNICATIONS

Photophysics of a fluorescent non-natural amino acid: p -Cyanophenylalanine

(2010) Arnaldo L. Serrano et al.   CHEMICAL PHYSICS LETTERS

Use of EF-Tu mutants for determining and improving aminoacylation efficiency and for purifying aminoacyl tRNAs with non-natural amino acids

(2010) T. Ohtsuki et al.   JOURNAL OF BIOCHEMISTRY

Thioamides as Fluorescence Quenching Probes: Minimalist Chromophores To Monitor Protein Dynamics

(2010) Jacob M. Goldberg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

A fluorophore ligase for site-specific protein labeling inside living cells

(2010) C. Uttamapinant et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

AnIn VivoCovalent TMP-Tag Based on Proximity-Induced Reactivity

(2009) Sarah S. Gallagher et al.   ACS Chemical Biology

Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

(2009) Ellen M. Sletten et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Probing Protein Folding Using Site-Specifically Encoded Unnatural Amino Acids as FRET Donors with Tryptophan

(2009) Shigeki J. Miyake-Stoner et al.   BIOCHEMISTRY

Interpretation ofp-Cyanophenylalanine Fluorescence in Proteins in Terms of Solvent Exposure and Contribution of Side-Chain Quenchers: A Combined Fluorescence, IR and Molecular Dynamics Study

(2009) Humeyra Taskent-Sezgin et al.   BIOCHEMISTRY

Single-Molecule Imaging of a Fluorescent Unnatural Amino Acid Incorporated Into Nicotinic Receptors

(2009) Rigo Pantoja et al.   BIOPHYSICAL JOURNAL

A Genetically Encoded Direct Sensor of Antibody-Antigen Interactions

(2009) Jeremy H. Mills et al.   CHEMBIOCHEM

Genetically encoded FRET-based biosensors for multiparameter fluorescence imaging

(2009) Haley J Carlson et al.   CURRENT OPINION IN BIOTECHNOLOGY

Genetic Incorporation of a Metal-Ion Chelating Amino Acid into Proteins as a Biophysical Probe

(2009) Hyun Soo Lee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Using Two Fluorescent Probes to Dissect the Binding, Insertion, and Dimerization Kinetics of a Model Membrane Peptide

(2009) Jia Tang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetic Incorporation of a Small, Environmentally Sensitive, Fluorescent Probe into Proteins inSaccharomyces cerevisiae

(2009) Hyun Soo Lee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Yeast Display Evolution of a Kinetically Efficient 13-Amino Acid Substrate for Lipoic Acid Ligase

(2009) Sujiet Puthenveetil et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The Use of Coumarins as Environmentally-Sensitive Fluorescent Probes of Heterogeneous Inclusion Systems

(2009) Brian Wagner   MOLECULES

Local environment perturbations in alpha1-antitrypsin monitored by a ratiometric fluorescent label

(2009) Christian Boudier et al.   PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

High-level cell-free synthesis yields of proteins containing site-specific non-natural amino acids

(2008) Aaron R. Goerke et al.   BIOTECHNOLOGY AND BIOENGINEERING

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY

Protein−Protein Interaction Detection in Vitro and in Cells by Proximity Biotinylation

(2008) Marta Fernández-Suárez et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanding the Substrate Tolerance of Biotin Ligase through Exploration of Enzymes from Diverse Species

(2008) Sarah A. Slavoff et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Azatryptophans endow proteins with intrinsic blue fluorescence

(2008) S. Lepthien et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA