Article
Engineering, Environmental
Pramod Kumar Mehta, Jongyong Jeon, Ki Ryu, See-Hyoung Park, Keun-Hyeung Lee
Summary: Ratiometric fluorescent peptidyl probe was synthesized and used for quantifying Pb(II) in aquatic environment and live cells. The probe displayed high selectivity and sensitivity towards Pb(II), with cell-permeability and rapid response. The study revealed that the probe formed a complex with Pb(II) and formed red-emissive nanoparticles and fibrils.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Zhuohang Li, Liyan Xiao, Xiaoqian Sun, Chenyao Luo, Rencheng Li, Wenbo Zhang, Zicheng Wang, Haibin Xiao, Wei Shu
Summary: A novel ESIPT ratio fluorescent probe (probe 1) has been proposed for detecting H2O2 in water environment and biosystems. Probe 1 exhibits short response time, high sensitivity, and a large Stokes shift to H2O2. It has been successfully used to detect H2O2 in actual water samples as well as endogenous and exogenous H2O2 in living cells, with wide applications in environmental and biological systems.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Physical
Vitor Brasiliense, Jean-Frederic Audibert, Tengfei Wu, Gilles Tessier, Pascal Berto, Fabien Miomandre
Summary: Surface modification by photo grafting is a promising strategy for preparing functional surfaces, but quantitative monitoring and control of the modifications remain challenging. This paper presents a label-free, cost-effective optical method based on wavefront sensing to track the evolution of grafted layers in real-time. The method allows for direct evaluation of the thickness of grafted patterns with high sensitivity and resolution, enabling in-depth kinetic analysis and characterization of different growth regimes.
Article
Chemistry, Applied
Xiuying Liu, Yu Wang, Zexin Zhang, Lijie Zhu, Xue Gao, Keli Zhong, Xiaofei Sun, Xuepeng Li, Jianrong Li
Summary: This study successfully developed a fluorescent sensing label that can detect fish freshness in real-time and visually. By utilizing ratiometric response and color change, the freshness of fish can be easily identified and the volatile amines can be quantitatively measured.
Article
Environmental Sciences
Xin Wang, Siyu Cheng, Caiyun Liu, Yan Zhang, Meijun Su, Xiaodi Rong, Hanchuang Zhu, Miaohui Yu, Wenlong Sheng, Baocun Zhu
Summary: Nickel resources are abundant, but excessive entry into the environment can cause pollution and health hazards. The detection methods for nickel ions are limited, and the mechanisms of nickel influence in organisms are still unclear. However, a ratiometric fluorescent probe Ra-Ni has been developed, which can specifically identify nickel ions and has the potential for sensitive detection. The probe has also been successfully used for imaging nickel ions in living cells and zebrafish, providing an effective tool for the study of physiological and pathological processes. The detection effect of nickel ions in actual water sample is also satisfactory, confirming the practicality of Ra-Ni in environmental applications.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Inorganic & Nuclear
Yanyan Han, Luyao Tang, Guangzhen Xu, Guiyi Yang, Yan Zhang, Haotian Xin, Yuan Cao, Duxia Cao, Zhiqiang Liu
Summary: A colorimetric and ratiometric fluorescent probe CCA was developed to detect hydrazine in water/soils, gas environment, and living cells. The probe showed a significant fluorescence change from red to blue upon the addition of hydrazine, and had low detection limit, large fluorescence change, and wide pH range. CCA probe could also be used to monitor hydrazine in actual samples and living cells.
INORGANIC CHEMISTRY COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Lihong Shi, Qianliang Wang, Caihong Zhang, Guomei Zhang, Yan Zhang, Chuan Dong, Shaomin Shuang
Summary: The study introduces tricolor-emission carbon dots (T-CDs) for ratiometric recognition of Al3+ and pyrophosphate ion (PPi), which can detect the concentrations of these ions in aqueous solution through ratiometric fluorescent changes and monitor their fluctuations in living cells in real time, demonstrating great potential in biological systems.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Huihui Tao, Zhao Zhang, Qiao Cao, Lingfei Li, Shihao Xu, Changlong Jiang, Yucheng Li, Yingying Liu
Summary: A novel carbon dot/Rhodamine B-based fluorescent probe was developed for highly sensitive and selective detection of nitrite (NO2-). The probe exhibited color changes from blue to orange under ultraviolet light in response to NO2-, with a detection limit as low as 67 nM in the range of 0 to 40 μM. A ratiometric fluorescent test paper was successfully prepared using the probe solution, demonstrating its feasibility for rapid and semi-quantitative detection of NO2- in real samples.
Article
Chemistry, Analytical
Ting Liu, Lixing Fu, Chenhui Yin, Meng Wu, Ligang Chen, Na Niu
Summary: A smartphone platform utilizing ratiometric fluorescence signals was developed for fast, sensitive, and on-site visual detection of silver ions. By constructing a ratiometric fluorescent probe with FITC and CdTe QDs, the color changes of the system were captured on a portable smartphone platform, enabling the visual analysis of Ag+ in environmental water samples at low detection limits.
MICROCHEMICAL JOURNAL
(2022)
Article
Environmental Sciences
Kuiyu Yi, Xiaoting Zhang, Lei Zhang
Summary: The development of a new phosphate optical sensor is of great practical significance. A ratiometric fluorescent probe and a smartphone-integrated colorimetric test paper sensing platform were successfully fabricated for detecting and recognizing phosphate. This innovative sensing platform provides a new strategy for intelligent online identification of important targets in biological and environmental samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Applied
Tingting Zhao, Chen Zhu, Shuai Xu, Xinghan Wu, Xiaotong Zhang, Yurong Zheng, Mengjiao Wu, Zhiwei Tong, Weijun Fang, Kui Zhang
Summary: A novel ratiometric fluorescent test paper was developed for qualitative and quantitative determination of ascorbic acid (AA), achieving continuous color changes from red to blue. The paper consists of blue-emitting carbon dots and red-emitting silica-coated quantum dots with a certain ratio of fluorescence intensity, providing a distinguishable color transition with the increasing AA concentration. This method allows easy and accurate detection of AA concentration through calculating the ratiometric fluorescent intensity or observing the color change by naked eyes.
Article
Chemistry, Analytical
Shiyu Chen, Beyza Vurusaner, Stephanie Pena, Chu T. Thu, Lara K. Mahal, Edward A. Fisher, James W. Canary
Summary: A fluorescent probe that selectively reacts with peroxynitrite, yielding over 200-fold change, effectively visualized fluctuations in peroxynitrite generation by arginase 1 in vivo and in vitro. This provides evidence that arginase 1 is a critical regulator of peroxynitrite.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jun Liao, Yi Fan, Suqi Deng, Zhicheng Yu, Ruidong Meng, Li Jia
Summary: A dual-channel fluorescent sensor was developed for levofloxacin detection. The sensor utilized the light-activatable oxidase mimic property of levofloxacin to generate free radicals and oxidize colorless compounds, resulting in visible color and fluorescence signal changes. The sensor exhibited low detection limits for levofloxacin and was successfully applied in the detection of levofloxacin in food samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Jie Wang, Wen-Jing Zhu, Jia-Yuan Zhang, Bin Qi, Jun-Peng Wang, Guang-Gang Gao, Lin-Lin Fan, Hong Liu
Summary: In this paper, a novel three-dimensional silver(I)-polyoxometalate complex, Ag3CrMo6, was designed and synthesized for the quantitative detection of formaldehyde. When exposed to formaldehyde, Ag3CrMo6 functions as a label-free SERS active substrate, showing enhanced signals attributed to Mo-O vibrations. By utilizing the constant Raman signals for Cr-O bonds as internal standards, the IMo-O/ICr-O values can be used as ratiometric SERS signals for the quantitative analysis of formaldehyde with a detection limit of 0.05 ng/L.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Huaiyou Chen, Ali Emre Celik, Angela Mutschler, Antoine Combes, Anne Runser, Andrey S. Klymchenko, Sebastien Lecommandoux, Christophe A. Serra, Andreas Reisch
Summary: Nanoprecipitation is a facile and efficient method for assembling loaded polymer nanoparticles in bioimaging and targeted drug delivery applications. Microfluidics is an attractive technique for reproducible and scalable synthesis. The study compares different types of microfluidic mixers in terms of nanoparticle formation and properties, and provides guidance for choosing the optimal microfluidic setup for producing nanomaterials for biomedical applications.
Article
Biology
Maria Rita Rodriguez-Luna, Nariaki Okamoto, Mahdi Al-Taher, Deborah S. Keller, Lorenzo Cinelli, Anila Hoskere Ashoka, Andrey S. Klymchenko, Jacques Marescaux, Michele Diana
Summary: Fluorescence-guided surgery has mainly focused on the NIR-I window in the past decade. However, there are limitations in the NIR-I window, such as limited penetration and scattering. Therefore, evaluating the performance of NIR-I dyes in the longer wavelength NIR-II window is crucial for expanding its application. This study used two fluorophores on three pigs and evaluated the mean fluorescence intensity (MFI) using commercially available NIR-I and NIR-II cameras. The results confirmed the feasibility of using NIR-I dyes in the NIR-II domain.
Article
Chemistry, Multidisciplinary
Ilya O. Aparin, Rui Yan, Remi Pelletier, Alexander A. Choi, Dmytro I. Danylchuk, Ke Xu, Andrey S. Klymchenko
Summary: This study proposes a concept of ON/OFF switchable probes for super-resolution fluorescence microscopy based on bright cyanine dyes. The new probes provide higher brightness and slower diffusion at the cell surface, improving the localization precision. The study validated the concept and demonstrated the observation of nanoscale cellular structures with enhanced dynamics.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Chemistry, Multidisciplinary
Andrey S. Klymchenko
Summary: Biomembranes, as lipid structures, play a crucial role in cellular processes. The development of chemical tools, such as fluorescent probes, for sensing and imaging biomembranes is a rapidly growing field. This Account focuses on advances in smart molecules for membrane targeting and sensing, with a particular emphasis on plasma membranes and organelles.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Lazare Saladin, Ophelie Dal Pra, Andrey S. S. Klymchenko, Pascal Didier, Mayeul Collot
Summary: Dual-emissive photoconvertible fluorophores (DPCFs) are useful for tracking labeled cells in bioimaging. The directed photooxidation-induced conversion (DPIC) mechanism allows efficient DPCFs to be obtained by conjugating a coumarin to aromatic singlet-oxygen reactive moieties (ASORMs). Pyrrole is a suitable ASORM that provides a high hypsochromic shift and fast conversion. By modifying pyrrole, we can tune the photoconversion properties of the DPCFs, leading to an improved dual emissive converter, SCP-Boc, with high brightness and a 63% enhanced photoconversion yield. SCP-Boc was successfully used for sequential cell photoconversion using laser scanning confocal microscopy.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Physical
Germain A. Brou, Gildas K. Gbassi, Ievgen Shulov, Aidar Seralin, Andrey S. Klymchenko, Thierry F. Vandamme, Nicolas Anton
Summary: In this study, we investigated the characterization and tracking of chemical reactions of amphiphile compounds on model interfaces. By using a custom-made surfactant and interfacial crosslinking through click chemistry, we observed changes in monolayer properties and dilatational elasticity. Through bulk phase exchange and washing, we specifically measured the interfacial effects of the entities adsorbed and trapped at the interface.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Nanoscience & Nanotechnology
Fei Liu, Nicolas Anton, Yosuke Niko, Andrey S. Klymchenko
Summary: Current biomedical applications of nanocarriers focus on controlled drug delivery, but this study proposes a different approach where toxic molecules are extracted from biological tissues using lipid nanoemulsion (NE) droplets. By designing a highly lipophilic amine (LipoAmine), cargo-aldehydes can be reacted and encapsulated inside lipid NEs through dynamic covalent chemistry. The formation of imine conjugates was validated and the NEs successfully extracted fluorescent dye and toxic aldehydes from cells, demonstrating the potential for developing detoxification platforms.
ACS APPLIED BIO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Deep Sekhar Biswas, Paraskevi Gaki, Elisabete Cruz Da Silva, Antoine Combes, Andreas Reisch, Pascal Didier, Andrey S. Klymchenko
Summary: By studying the FRET phenomenon between fluorescent organic nanoparticles, it is found that the efficiency does not follow the Forster law and can achieve high efficiency at long distances. This opens up the possibility of using advanced optical nanomaterials for amplified FRET-based biosensing.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Reimi Pelletier, Dmytro I. Danylchuk, Hela Benaissa, Fanny Broch, Romain Vauchelles, Arnaud Gautier, Andrey S. Klymchenko
Summary: Genetically encoded protein labels combined with polarity-sensitive fluorescent probes provide information about the nanoscale environments of proteins within subcellular compartments. A solvatochromic HaloTag probe, NR12-Halo, specifically labels a variety of proteins localized in different cell compartments and distinguishes proteins within lipid membranes. This probe also reveals changes in protein environments during their life cycle, heterogeneity in membrane protein polarity, and the effects of mechanical stress and fatty acid diet on protein nanoenvironments. The solvatochromic HaloTag probe is a promising tool for studying protein interactions and nanoscale environments within subcellular structures.
ANALYTICAL CHEMISTRY
(2023)
Editorial Material
Chemistry, Multidisciplinary
L. Saladin, O. Dal Pra, A. S. S. Klymchenko, P. Didier, M. Collot
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Physical
Tesla Yudhistira, Elisabete Cruz Da Silva, Antoine Combes, Maxime Lehmann, Andreas Reisch, Andrey S. S. Klymchenko
Summary: This study reports a method of antibody labeling using biotinylated zwitterionic dye-loaded polymeric nanoparticles. The prepared biotinylated nanoparticles are small (14 nm) and bright, capable of carrying a large amount of cationic rhodamine dye. The method significantly improves fluorescence imaging of target epidermal growth factor receptors and becomes a useful tool in the high-sensitivity detection of disease biomarkers.
Article
Materials Science, Multidisciplinary
Deep Sekhar Biswas, Nina Melnychuk, Caterina Severi, Pascal Didier, Andrey S. Klymchenko
Summary: Light-harvesting is a fundamental process in nature that drives the development of artificial systems for various applications. Researchers have discovered that using blank hydrophobic salts can suppress dye aggregation within nanoparticles, improving their fluorescence properties and enabling efficient energy transfer and antenna effects.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Lucie Haye, Nour Fayad, Richard C. Knighton, Antoine Combes, Olivier Jeannin, Andrey Klymchenko, Thibault Gallavardin, Niko Hildebrandt, Loic J. Charbonniere, Andreas Reisch
Summary: This study investigates the physicochemical and photophysical properties of Re diimine complexes and Cy5 dyes coencapsulated in polymer nanoparticles (NPs), and their compatibility for bioimaging. The results show that encapsulation significantly enhances the photoluminescence brightness and lifetimes of Re complexes, while coencapsulation of Cy5 leads to bright near-infrared emission upon Re complex excitation. Furthermore, the Re-Cy5 NPs exhibit high-contrast photoluminescence imaging in mammalian cells.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Review
Chemistry, Multidisciplinary
Anila Hoskere Ashoka, Ilya O. Aparin, Andreas Reisch, Andrey S. Klymchenko
Summary: Brightness is crucial for (bio)molecular detection and optical bioimaging. This tutorial review provides definitions of brightness and describes the major approaches to its analysis. It also discusses the current chemical methods to combat Aggregation-Caused Quenching and compares the brightness and other properties of different fluorescent organic nanoparticles. The importance of brightness in biological applications is also analyzed.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Caterina Severi, Satu Lahtinen, Jaana Rosenberg, Andreas Reisch, Tero Soukka, Andrey S. Klymchenko
Summary: The study investigates controlling the aggregation behavior of dyes in polymeric nanoparticles by using bulky lanthanide cations to develop bright luminescent multifunctional nanomaterials.