Article
Food Science & Technology
Zhebin Chen, Dasong Liu, Xue Tang, Ying Cui, Wei Hu, Joe M. Regenstein, Peng Zhou
Summary: Sea cucumber enzymatic hydrolysates (SCEH) can inhibit osteoclast generation via the NF-kappa B pathway, reduce inflammation related to osteoporosis, and improve bone metabolism, thereby increasing bone density and relieving osteoporosis.
Review
Endocrinology & Metabolism
Yan Zhang, Jingqi Liang, Peilong Liu, Qiong Wang, Liang Liu, Hongmou Zhao
Summary: With the increasing incidence of cancer, tumor bone metastasis has become more common among patients. The RANK/RANKL/OPG system and osteoclasts play important roles in bone homeostasis and tumor bone metastasis formation, and inhibiting this system can prevent metastasis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Dan Xu, Hong-Jiao Gao, Chun-Yan Lu, Hao-Ming Tian, Xi-Jie Yu
Summary: This study found that in mice with thyrotoxicosis, treatment with vitamin D can inhibit bone resorption and improve bone density and trabecular bone structure by increasing the ratio of OPG to RANKL and upregulation of Wnt/beta-catenin expression.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, General & Internal
Margarita Ivanova, Julia Dao, Lauren Noll, Jacqueline Fikry, Ozlem Goker-Alpan
Summary: This study found that TRAP5b levels were elevated in GD patients and correlated with GD biomarkers. Additionally, an increase in RANKL and RANKL/OPG ratio was associated with osteopenia in GD, suggesting that TRAP5b may be a potential biomarker for bone disorders in GD.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Polymer Science
Laura Ricciotti, Antonio Apicella, Valeria Perrotta, Raffaella Aversa
Summary: With advancements in bone tissue engineering, the development of innovative biomaterials is crucial for improving bone healing. This review thoroughly examines the current applications and future potential of geopolymers in bone tissue engineering. Traditional bioscaffold materials are also compared, analyzing their strengths and weaknesses. The concerns regarding the widespread use of alkali-activated materials as biomaterials and the potential of geopolymers as ceramic biomaterials are discussed.
Article
Dentistry, Oral Surgery & Medicine
Jianli Zhou, Lijia Guo, Yanji Yang, Yi Liu, Chen Zhang
Summary: This study evaluated the effects of orthodontic forces on dental pulp and periodontal tissues. It was found that heavy orthodontic forces activated osteoclasts and resulted in external root resorption by upregulating RANKL expression in rat periodontal tissues. In contrast, there was no significant change in RANKL expression in dental pulp tissue under heavy orthodontic forces, which prevented osteoclast activation and internal root resorption. Additionally, orthodontic force stimulation tended to promote tertiary dentin formation in dental pulp.
Article
Pharmacology & Pharmacy
Samaa M. El-Mahroky, Mahitab M. Nageeb, Dalia A. Hemead, Enas G. Abd Allah
Summary: This study aimed to investigate the defensive effect of the antidepressant agomelatine against steroid-induced osteoporosis. The results showed that agomelatine ameliorated osteoporosis by activating SIRT1 and restoring biochemical parameters to normal levels, improving histopathological changes.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2023)
Review
Endocrinology & Metabolism
Nobuyuki Udagawa, Masanori Koide, Midori Nakamura, Yuko Nakamichi, Teruhito Yamashita, Shunsuke Uehara, Yasuhiro Kobayashi, Yuriko Furuya, Hisataka Yasuda, Chie Fukuda, Eisuke Tsuda
Summary: Osteoclasts and osteoblasts play essential roles in bone remodeling, with the regulation of RANKL and OPG expression being a key mechanism. The balance between these factors is crucial for maintaining bone density and preventing bone loss.
JOURNAL OF BONE AND MINERAL METABOLISM
(2021)
Article
Endocrinology & Metabolism
Hyo Shik Shin, Hyun Jin Sun, Young Mi Whang, Young Joo Park, Do Joon Park, Sun Wook Cho
Summary: Metformin significantly reduced the viabilities of thyroid cancer cells and delayed tumor growth in bone metastasis. In the microenvironment of bone metastasis, metformin inhibited cancer cell growth, blocked cancer cell-induced osteoblastic RANKL production, which further activated osteoclastogenesis, and directly reduced osteoclast differentiation.
Article
Engineering, Multidisciplinary
K. Pluta, W. Florkiewicz, D. Malina, K. Rudnicka, S. Michlewska, J. B. Krolczyk, A. Sobczak-Kupiec
Summary: This paper focuses on the measurement methods of UV cross-linked hydroxyapatite-polymers composites and their effects on the structure, mechanical properties, and biocompatibility. Results show that these materials are non-cytotoxic and do not alter cell morphology. The addition of certain components leads to increased deformation and swelling capacity in the composites.
Article
Biotechnology & Applied Microbiology
Xiao-Liang Liu, Chuan-Jian Zhang, Jing-Jing Shi, Qin-Fei Ke, Yu-Wei Ge, Zhen-An Zhu, Ya-Ping Guo
Summary: Autogenous bone grafting is limited in treating critical bone defects due to its drawbacks, thus effective bone substitutes are needed. Inspired by the nacre architecture, a layered nano-hydroxyapatite/chitosan composite scaffold was designed and fabricated. The scaffold promoted osteogenesis and inhibited osteoclast activation, offering a promising platform for bone regeneration.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Metallurgy & Metallurgical Engineering
Hui Fang, Shicheng Zhou, Xiaoyun Qi, Chenxi Wang, Yanhong Tian
Summary: In this study, biomimetic bone-active coatings composed of inorganic nano-hydroxyapatite (nHA) and organic silk fibroin (SF) were deposited layer-by-layer on a magnesium-zinc-calcium (Mg-Zn-Ca) alloy surface using a controllable ultrasonic spray method. Plasma activation was used to pretreat the magnesium surfaces, enhancing the adhesion of the coatings. The nHA/SF composite coatings exhibited excellent mechanical properties and adhesion force. The use of hybrid plasma of oxygen and nitrogen (O2/N2 plasma) resulted in a uniformly covered and robust nHA/SF composite coating with superior corrosion resistance and biological performance. Additionally, secondary oxygen plasma treatment of the coatings promoted hydrophilicity and self-repair, leading to improved cell adhesion, proliferation, spreading, and differentiation.
JOURNAL OF MAGNESIUM AND ALLOYS
(2023)
Review
Materials Science, Multidisciplinary
Richard Drevet, Joel Faure, Hicham Benhayoune
Summary: This review discusses the design of bioactive calcium phosphate coatings on metallic substrates for bone implants. These coatings create a strong bond between the implants and surrounding bone tissue, enhancing the implant's lifespan and functionality. The article explores different calcium phosphate compounds, deposition processes, and their impact on the bioactivity and performance of bone implants.
Article
Agriculture, Dairy & Animal Science
Oriana M. Koehler, Walter Gruenberg, Nadine Schnepel, Alexandra S. Muscher-Banse, Abbas Rajaeerad, Juergen Hummel, Gerhard Breves, Mirja R. Wilkens
Summary: The study found that dietary phosphate restriction did not lead to hypophosphatemia, likely due to compensation by bone mobilization, as evidenced by increased serum concentrations of a resorption marker and altered gene expression in bone tissue. Additionally, the expression of fibroblast growth factor 23, a bone-derived factor involved in regulating vitamin D metabolism, was significantly reduced with dietary phosphate restriction. Several genes related to vitamin D metabolism and plasma concentrations of 1,25-dihydroxycholecalciferol were associated with serum phosphate concentrations.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2021)
Review
Oncology
Junjie Lu, Desheng Hu, Yan Zhang, Chen Ma, Lin Shen, Bo Shuai
Summary: Denosumab, a fully humanized monoclonal neutralizing antibody, inhibits bone resorption by competitively binding with RANKL and blocking the activation of the RANK/RANKL/OPG signaling pathway. It is used to treat metabolic bone diseases and has potential in the treatment of osteoarthritis, bone tumors, and other autoimmune diseases. However, more research is needed to understand its mechanism of action and optimize its clinical use for bone metastasis of malignant tumors.
FRONTIERS IN ONCOLOGY
(2023)
Review
Dentistry, Oral Surgery & Medicine
Vittorio Moraschini, Tiago Rocha de Queiroz, Suelen Cristina Sartoretto, Daniel Costa Ferreira de Almeida, Monica Diuana Calasans-Maia, Rafael Seabra Louro
Summary: This study aimed to compare the survival and complication rates of zygomatic implants (ZI) with conventional implants (CI) in the long term. The results showed that there was no significant difference in the survival rates between ZI and CI, and the most common biological complication was sinusitis, especially in the IZI technique.
CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Gabriela de Souza Castro, Wanderson de Souza, Thais Suelen Mello Lima, Danielle Cabral Bonfim, Jacques Werckmann, Braulio Soares Archanjo, Jose Mauro Granjeiro, Ana Rosa Ribeiro, Sara Gemini-Piperni
Summary: This study compares the effects of different concentrations of TiO2 NPs on the differentiation and mineralized matrix production of human osteoblasts in a monolayer or 3D model. The internalization of nanoparticles and their impact on cell morphology are evaluated. The results demonstrate a dose-dependent increase in mineralized matrix in both the monolayer and 3D models, with higher levels observed in the 3D model after high exposure to TiO2 NPs, suggesting its potential as a model for testing the effect on bone osteointegration.
Article
Chemistry, Multidisciplinary
Ana Carolina Batista Brochado, Daniela Costa Silva, Joice Correa da Silva, Adam Lowenstein, Vinicius Schott Gameiro, Elena Mavropoulos, Carlos F. Mourao, Gutemberg Gomes Alves
Summary: This study described the characterization of a 3D spheroid model of MC3T3-E1 murine preosteoblasts for testing bone-substitute materials and investigated the suitability of several cell viability tests. The spheroids showed structural stability, compact surface, and high potential for mineralization but had a reduction in size over time. Colorimetric tests did not correlate with cell density in the 3D model, while the LDH test achieved appropriate optical density and high correlation with cell density, demonstrating the importance of careful selection of test methods for material testing with 3D bone cell models.
APPLIED SCIENCES-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Carlos Fernando Mourao, Adam Lowenstein, Rafael Coutinho Mello-Machado, Shahram Ghanaati, Nelson Pinto, Tomoyuki Kawase, Gutemberg Gomes Alves, Michel Reis Messora
Summary: Experimental research plays a vital role in advancing medical knowledge and improving patient outcomes. Platelet-rich fibrin (PRF) has gained attention for its potential in tissue regeneration and wound healing. Animal models, such as rabbits and rats, have been used to study PRF and its applications. Standardizing animal models and techniques is crucial for bridging the gap between lab research and clinical applications.
BIOENGINEERING-BASEL
(2023)
Article
Chemistry, Analytical
Elisa Rosa dos Santos, Evaldo Cesar Cavalcante Rodrigues, Jose Mauro Granjeiro
Summary: The biotechnology policy encourages the establishment of institution networks for bioassays and preclinical studies to promote good laboratory practice (GLP) principles. Trained professionals are essential to understand GLP, develop procedures, and implement quality management requirements. The use of multicriteria decision aid (MCDA) can assist in managing and implementing the quality system. This research demonstrates the perception of GLP difficulty levels and identifies efforts to improve understanding through MCDA analysis.
ACCREDITATION AND QUALITY ASSURANCE
(2023)
Review
Dentistry, Oral Surgery & Medicine
Vittorio Moraschini, Richard J. Miron, Carlos Fernando de Almeida Barros Mourao, Rafael Seabra Louro, Anton Sculean, Luiz Afonso Morgenstern da Fonseca, Monica Diuana Calasans Maia, Jamil Awad Shibli
Summary: This systematic review evaluated the antimicrobial potential of different types of platelet-rich fibrin (PRF) used in regenerative treatments. The findings showed that all types of PRF had significant antimicrobial action, with greater efficacy against bacteria compared to fungi. Future studies analyzing the clinical effect of PRF are important.
PERIODONTOLOGY 2000
(2023)
Article
Engineering, Biomedical
Kayvon Javid, Carlos Fernando Mourao, Rafael Coutinho Mello-Machado, Suelen Cristina Sartoretto, Madelaine Torres, Emanuelle Stellet Lourenco, Paulo Emilio Correa Leite, Jose Mauro Granjeiro, Gutemberg Gomes Alves, Monica Diuana Calasans-Maia
Summary: This study evaluated the clinical and biochemical effects of heated serum albumin with liquid PRF (Alb-PRF) and L-PRF in socket healing after mandibular third molar extraction. The results showed that Alb-PRF significantly reduced swelling on day seven and had lower levels of pro-inflammatory cytokines compared to L-PRF. Alb-PRF promoted socket healing by reducing edema and the release of inflammatory cytokines.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Engineering, Biomedical
Renata de Lima Barbosa, Emanuelle Stellet Lourenco, Julya Vittoria de Azevedo dos Santos, Neilane Rodrigues Santiago Rocha, Carlos Fernando Mourao, Gutemberg Gomes Alves
Summary: Platelet-rich fibrin (PRF) is an autologous approach for tissue regeneration that promotes cell interaction and growth factor release. In vitro evidence suggests that PRF has a positive effect on bone cell behavior, including proliferation, differentiation, and mineralization. This reinforces PRF's therapeutic potential in wound healing and bone regeneration.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Medicine, General & Internal
Roberto Sacco, Oladapo Akintola, Nicola Sacco, Alessandro Acocella, Monica Diuana Calasans-Maia, Massimo Maranzano, Sergio Olate
Summary: The purpose of this study is to investigate the efficacy of human amniotic membranes (hAM) as a treatment modality for medication-induced osteonecrosis of the jaw (MRONJ). Based on a systematic search of multiple databases, the authors found that treatment with hAM significantly reduced the recurrence rate of MRONJ. Although the data is limited, this study suggests that using hAM for MRONJ treatment may be a viable option.
MEDICINA-LITHUANIA
(2023)
Article
Materials Science, Biomaterials
Wanderson de Souza, Sara G. Piperni, Liliana Grenho, Luis A. Rocha, Jose M. Granjeiro, Sonia A. Melo, Maria H. Fernandes, Ana R. Ribeiro
Summary: Titanium (Ti) and its alloys are widely used in total joint replacement, but evidence shows that their surface degradation due to corrosion and wear processes can contribute to osteolysis and implant loosening. This study investigates the impact of titanium dioxide nanoparticles (TiO2 NPs) on osteoblast-derived exosome (Exo) biogenesis and the communication between osteoblasts and human mesenchymal stem/stromal cells (HMSCs). We found that TiO2 NPs stimulate the secretion of Exos from immature and mature osteoblasts, which have different proteomic cargo. Functional tests showed that Exos derived from both types of osteoblasts decrease the osteogenic differentiation of HMSCs. These findings have clinical relevance as wear debris can disrupt extracellular communication and lead to joint failure.
BIOMATERIALS SCIENCE
(2023)
Article
Medicine, General & Internal
Roberto Sacco, Suelen Cristina Sartoretto, Rodrigo Figueiredo de Brito Resende, Jose de Albuquerque Calasans-Maia, Alexandre Malta Rossi, Victor Hugo de Souza Lima, Carlos Fernando de Almeida Barros Mourao, Jose Mauro Granjeiro, Julian Yates, Monica Diuana Calasans-Maia
Summary: Medication-related osteonecrosis of the jaw (MRONJ) is a severe adverse side effect of certain drugs and is associated with invasive dental procedures. Previous studies have investigated preventive strategies, but no investigation has evaluated the therapeutic effect of local drug-delivery technology. This study aims to evaluate the efficacy of hydroxyapatite-containing doxycycline (HADOX) in rats with high-risk MRONJ development.
MEDICINA-LITHUANIA
(2023)
