Article
Biochemistry & Molecular Biology
Yi-Jen Peng, Jeng-Wei Lu, Chian-Her Lee, Herng-Sheng Lee, You-Hsiang Chu, Yi-Jung Ho, Feng-Cheng Liu, Chun-Jung Huang, Chia-Chun Wu, Chih-Chien Wang
Summary: Cardamonin has been shown to significantly reduce inflammation in chondrocyte cells, inhibiting the release of inflammatory mediators and pro-inflammatory proteins. It also activates the Nrf2 signaling pathway and increases levels of antioxidant proteins, showing potential as an Nrf2 activator for treating and preventing OA related to inflammation and oxidative stress.
Article
Education, Scientific Disciplines
Takashi Ishijima, Kazuyuki Nakajima
Summary: Through utilizing an animal model with LPS-induced rat brain, the study revealed that microglia induce different inflammatory cytokines through specific combinations of MAPKs and by the presence or absence of nitric oxide.
Article
Multidisciplinary Sciences
Xiaobo Guo, Zhenyuan Wu
Summary: The study revealed that treatment with GABARAP protein promoted cell proliferation and osteogenic differentiation of BMSCs, while reducing apoptosis and inflammatory factor expression. However, the protective effects of GABARAP were attenuated by the autophagy inhibitor 3-MA.
SCIENTIFIC REPORTS
(2021)
Article
Nanoscience & Nanotechnology
Meimei Zhang, Runlin Zhang, Hui Chen, Xiaofeng Zhang, Yilei Zhang, Haining Liu, Chen Li, Yunhua Chen, Qing Zeng, Guozhi Huang
Summary: In this study, a thermosensitive injectable supra-molecular hybrid hydrogel was developed to deliver interleukin-1β-stimulated exosomes derived from bone marrow stromal cells (BMSCs) for neuroinflammation inhibition and neurological recovery after cerebral ischemia-reperfusion injury (CIRI). The hydrogel showed self-healing and injectable properties, as well as high biocompatibility and softness similar to tissue. The released exosomes effectively reduced inflammatory responses in microglia cells and improved retention in the ischemic core area, leading to reduced glial scar formation and neuronal loss in vivo.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Orthopedics
Nan Yao, Sha Peng, Huai Wu, Wengang Liu, Dake Cai, Dane Huang
Summary: This study demonstrates that PVT1, acting as a competing endogenous RNA (ceRNA) of miR-140, promotes the expressions of ADAMTS-5 and MMP-13 in osteoarthritis, leading to the aggravation of ECM degradation.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yang Yue, Sun Zhibo, Liu Feng, Yuanzhang Bai, Wu Fei
Summary: The study demonstrates that SNHG5 can protect chondrocytes from inflammatory response and reduce extracellular matrix degradation by modulating the miR-181a-5p/TGFBR3 axis.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Laura Darie-Ion, Madhuri Jayathirtha, Gabriela Elena Hitruc, Marius-Mihai Zaharia, Robert Vasile Gradinaru, Costel C. Darie, Aurel Pui, Brindusa Alina Petre
Summary: Through combining different analytical methods, zein protein was extracted including mass spectrometry, SDS-PAGE, AFM, and FTIR-ATR. The use of ultrasonic extraction method with 65% ethanol extraction buffer and 710 mu m maize granulation yielded the highest protein extraction. The extracted zein was mainly alpha-zein monomer according to SDS-PAGE analysis and the main component in maize flour extract was alpha-zein PMS2 protein based on MS analysis. A homogeneous content of extracted zein could potentially be used for future medical applications according to AFM studies.
Article
Immunology
Sandy ElSayed, Gregory D. Jay, Ralph Cabezas, Marwa Qadri, Tannin A. Schmidt, Khaled A. Elsaid
Summary: The study revealed that monocytes from acute gout patients exhibited higher phagocytic activation and ROS generation under basal conditions, as well as increased IL-1 beta secretion upon MSU stimulation. rhPRG4 showed better inhibition of phagocytic activation in monocytes from both normal and gout patients compared to IL-1RA, and both treatments effectively reduced IL-1 beta secretion.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jie Yang, Lutian Yao, Yuxuan Li, Lin Yuan, Ruoxi Gao, Ran Huo, Hui Zhang, Liping Xia, Hui Shen, Jing Lu
Summary: IL-35 regulates differentiation and expression of pro-angiogenic molecules in IL-1 beta stimulated SW1353 cells via Th17/IL-17 related signaling pathways.
MOLECULAR IMMUNOLOGY
(2022)
Article
Immunology
Miao Zhang, Leming Mou, Shiwei Liu, Fengyi Sun, Mingzhi Gong
Summary: The study revealed that circ_0001103 alleviated IL-1 beta-induced chondrocyte injuries by regulating the miR-375/SIRT1 axis, suggesting circ_0001103 as a potential promising biomarker in the pathogenesis of OA.
CLINICAL IMMUNOLOGY
(2021)
Article
Hematology
Wuwei Rong, Chenchen Liu, Xiaoming Li, Naifu Wan, Lijiang Wei, Wentong Zhu, Peiyuan Bai, Ming Li, Yangjing Ou, Fang Li, Lingxia Wang, Xuanhui Wu, Jianling Liu, Mingyan Xing, Xiaoming Zhao, Han Liu, Haibing Zhang, Ankang Lyu
Summary: This study reveals the role of caspase-8 in regulating IL-1 beta activation in macrophages and its involvement in pulmonary arterial hypertension (PAH). Caspase-8 promotes inflammatory cell infiltration and pulmonary arterial smooth muscle cell proliferation via NLRP3/IL-1 beta activation, contributing to the pathogenesis of PAH.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kseniia Markova, Valentina Mikhailova, Yulia Milyutina, Andrey Korenevsky, Anastasia Sirotskaya, Veronika Rodygina, Elizaveta Tyshchuk, Polina Grebenkina, Andrey Simbirtsev, Sergey Selkov, Dmitry Sokolov
Summary: Microvesicles (MVs) are plasma extracellular vesicles produced by different cells, which can mediate intercellular communication and modulate cell functions. Studies show that MVs derived from NK cells have the potential to decrease the proliferative activity of endothelial cells, induce cell death, and reduce the expression of specific surface molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Orthopedics
Hyun Sook Hwang, Mi Hyun Lee, Dong Jin Go, Hyun Ah Kim
Summary: The study suggests that the sympathetic nervous system plays a significant role in the modulation of cartilage metabolism induced by interleukin-1beta (IL-1β). Beta-adrenergic receptors (β-ARs) exhibit a regulatory effect on cartilage catabolism induced by IL-1β, with beta-AR antagonism enhancing catabolic signals and norepinephrine antagonizing the catabolic response. Additionally, beta-AR activity affects IL-1β-stimulated signaling pathways in chondrocytes, suggesting a connection between beta-AR signaling and cartilage metabolism.
BMC MUSCULOSKELETAL DISORDERS
(2021)
Article
Psychiatry
Rajesh Das, Md. Prova Zaman Emon, Mohammad Shahriar, Zabun Nahar, Sardar Mohammad Ashraful Islam, Mohiuddin Ahmed Bhuiyan, Sheikh Nazrul Islam, Md. Rabiul Islam
Summary: The study found that serum IL-1 beta and TNF-alpha levels are increased in MDD patients and are positively correlated with the severity of depression. Elevated levels of serum IL-1 beta and TNF-alpha could potentially be used as risk assessment indicators for depression.
PSYCHIATRY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Hae-Ri Lee, Young-Jin Jeong, Joong-Woon Lee, JooYeon Jhun, Hyun Sik Na, Keun-Hyung Cho, Seok Jung Kim, Mi-La Cho, Tae-Hwe Heo
Summary: The study revealed that tannic acid (TA) inhibits IL-1 beta-induced inflammation and matrix degradation in human OA chondrocytes by blocking IL-1 beta-IL-1R1 interaction. In addition, TA showed protective effects in a rat OA model by reducing pain, cartilage degradation, and IL-1 beta-mediated inflammation.
Letter
Rheumatology
Francis Berenbaum, Francisco J. Blanco, Ali Guermazi, Kenji Miki, Takaharu Yamabe, Lars Viktrup, Rod Junor, William Carey, Mark T. Brown, Christine R. West, Kenneth M. Verburg
ANNALS OF THE RHEUMATIC DISEASES
(2022)
Article
Biochemical Research Methods
Nuria Colome, Joaquin Abian, Kerman Aloria, Jesus M. Arizmendi, Silvia Barcelo-Batllori, Sophie Braga-Lagache, Odile Burlet-Schiltz, Montse Carrascal, J. Ignacio Casal, Eduard Chicano-Galvez, Cristina Chiva, Luis Felipe Clemente, Felix Elortza, Josep M. Estanyol, Joaquin Fernandez-Irigoyen, Patricia Fernandez-Puente, Maria Jose Fidalgo, Carine Froment, Manuel Fuentes, Carlos Fuentes-Almagro, Marina Gay, Alexandre Hainard, Manfred Heller, Maria Luisa Hernandez, Nieves Ibarrola, Ibon Iloro, Thomas Kieselbach, Antonio Lario, Marie Locard-Paulet, Anabel Marina-Ramirez, Luna Martin, Esperanza Morato-Lopez, Javier Munoz, Rosana Navajas, M. Antonia Odena, Leticia Odriozola, Eliandre de Oliveira, Alberto Paradela, Carla Pasquarello, Vivian de los Rios, Cristina Ruiz-Romero, Eduard Sabido, Manuel Sanchez del Pino, Jaime Sancho, Enrique Santamaria, Christine Schaeffer-Reiss, Justine Schneider, Carolina de la Torre, M. Luz Valero, Marta Vilaseca, Shuai Wu, Linfeng Wu, Pilar Ximenez de Embun, Francesc Canals, Fernando J. Corrales
Summary: Global analysis of protein phosphorylation using mass spectrometry has become a powerful tool in biological and biomedical research, but it presents challenges such as low stoichiometry and the need for residue-specific information. The Proteomics Multicentric Experiment 11 (PME11) was launched to investigate the performance of phosphoprotein analysis protocols, with a reference sample distributed to 31 laboratories for evaluation. The results indicate the suitability of the PME11 reference sample for benchmarking and optimizing phosphoproteomics strategies while evaluating intra and inter laboratory performance.
JOURNAL OF PROTEOMICS
(2022)
Review
Biochemistry & Molecular Biology
Maria Jose Lopez-Armada, Jennifer Adriana Fernandez-Rodriguez, Francisco Javier Blanco
Summary: Controlling excessive mitochondrial oxidative stress can provide new targets for the treatment of chronic inflammation or diseases such as rheumatoid arthritis (RA). Mitochondrial alterations play a role in autoimmune diseases through the interaction between metabolism and innate immunity. Mitochondrial dysfunction can activate inflammatory pathways and impact cell death and epigenetic regulation. Additionally, there may be a relationship between dietary components and the onset and progression of RA.
Article
Orthopedics
W. Wirth, S. Maschek, A. C. A. Marijnissen, A. Lalande, F. J. Blanco, F. Berenbaum, L. A. van de Stadt, M. Kloppenburg, I. K. Haugen, C. H. Ladel, J. Bacardit, A. Wisser, F. Eckstein, F. W. Roemer, F. P. J. G. Lafeber, H. H. Weinans, M. Jansen
Summary: The study aims to investigate the test-retest precision of cartilage thickness and report longitudinal changes in the IMI-APPROACH cohort. The machine-learning-estimated structural progression score (s-score) was not predictive of cartilage thickness loss.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Medicine, General & Internal
Cristina Ruiz-Romero, Patricia Fernandez-Puente, Lucia Gonzalez, Anna Illiano, Lucia Lourido, Rocio Paz, Patricia Quaranta, Eva Perez-Pampin, Antonio Gonzalez, Francisco J. Blanco, Valentina Calamia
Summary: This study utilizes a proteomic biomarker pipeline to analyze the molecular changes associated with the RF and ACPA status of rheumatoid arthritis patients. The results identify specific proteins that show altered abundance in relation to RF and ACPA status, and two putative biomarkers, A1AG1 and HPT, are validated as increased in the double seropositive group. These findings provide valuable information for the stratification and personalized medicine of rheumatoid arthritis patients.
FRONTIERS IN MEDICINE
(2022)
Article
Orthopedics
Frank W. Roemer, Mylene Jansen, Anne C. A. Marijnissen, Ali Guermazi, Rafael Heiss, Susanne Maschek, Agnes Lalande, Francisco J. Blanco, Francis Berenbaum, Lotte A. van de Stadt, Margreet Kloppenburg, Ida K. Haugen, Christoph H. Ladel, Jaume Bacardit, Anna Wisser, Felix Eckstein, Floris P. J. G. Lafeber, Harrie H. Weinans, Wolfgang Wirth
Summary: The IMI-APPROACH cohort used MRI evaluation to study the characteristics of knee osteoarthritis, and found that the baseline prevalence and change of features were more common in knees with radiographic OA.
BMC MUSCULOSKELETAL DISORDERS
(2022)
Article
Biochemistry & Molecular Biology
Carlos Vaamonde-Garcia, Emma Capelo-Mera, Noelia Florez-Fernandez, Maria Dolores Torres, Beatriz Rivas-Murias, Rosa Mejide-Failde, Francisco J. Blanco, Herminia Dominguez
Summary: This study analyzed the therapeutic potential of crude fucoidans in chondrocytes and found that they have partial effectiveness in alleviating inflammation and oxidative stress associated with osteoarthritis, but do not attenuate chondrocyte senescence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Manuel Fuentes, Cristina Ruiz-Romero, Sara Misiego, Pablo Juanes-Velasco, Alicia Landeira-Vinuela, Adrian Torres-Roda, Hector Lorenzo-Gil, Maria Gonzalez-Gonzalez, Angela P. Hernandez, Lucia Lourido, Ronald Sjoberg, Elisa Pin, Javier de Las Rivas, Jose Manuel Sanchez-Santos, Peter Nilsson, Francisco J. Blanco
Summary: Rheumatic diseases are common and complex conditions with low sensitivity to clinical diagnosis. This study developed an early diagnosis method for personalized treatment based on the identification of relevant proteins associated with the specific pathologies. By utilizing functional proteomics screening, a panel of 30 serum proteins has been identified as potential biomarkers for rheumatic diseases such as osteoarthritis, rheumatoid arthritis, and psoriatic arthritis.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Rheumatology
Filip Van den Bosch, James Cheng-Chung Wei, Peter Nash, Francisco J. Blanco, Daniela Graham, Chuanbo Zang, Edmund Arthur, Cecilia Borlenghi, Vassilis Tsekouras, Bonnie Vlahos, Atul Deodhar
Summary: This study investigated the withdrawal and retreatment of etanercept in patients with nr-axSpA achieving inactive disease. The results showed that approximately 25% of patients maintained inactive disease for 40 weeks after discontinuing etanercept. Baseline absence of MRI sacroiliitis and high hs-CRP predicted response maintenance after etanercept withdrawal.
JOURNAL OF RHEUMATOLOGY
(2023)
Review
Chemistry, Medicinal
Carmen Nunez-Carro, Margarita Blanco-Blanco, Karla Mariuxi Villagran-Andrade, Francisco J. Blanco, Maria C. de Andres
Summary: In this study, we reviewed the current understanding of epigenetic mechanisms in relation to the pathogenesis of OA, particularly in articular cartilage. Evidence suggests that dysregulation of crucial cartilage molecules is a result of aberrant epigenetic regulatory mechanisms, which contribute to the development and progression of OA. This provides an opportunity to explore new therapeutic targets and novel biomarkers for the disease.
Article
Rheumatology
Alejandro Duran-Sotuela, Mercedes Fernandez-Moreno, Victoria Suarez-Ulloa, Jorge Vazquez-Garcia, Sara Relano, Tamara Hermida-Gomez, Vanesa Balboa-Barreiro, Lucia Lourido-Salas, Valentina Calamia, Patricia Fernandez-Puente, Cristina Ruiz-Romero, Juan Fernandez-Tajes, Carlos Vaamonde-Garcia, Maria C. de Andres, Natividad Oreiro, Francisco J. Blanco, Ignacio Rego-Perez
Summary: This study aimed to investigate the genetic variants in mitochondrial DNA (mtDNA) associated with the risk of rapid progression of knee osteoarthritis (OA) and evaluate their functional significance using a cellular model. The results showed that the mtDNA variant m.16519C was associated with an increased risk of rapid progression of knee OA. The cellular model study further revealed mitochondrial dysfunction and impaired autophagy in cells harboring this variant, which were related to inflammation in OA.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Orthopedics
I. Lorenzo-Gomez, U. Nogueira-Recalde, C. Garcia-Dominguez, N. Oreiro-Villar, M. Lotz, J. A. Pinto-Tasende, F. J. Blanco, B. Carames
Summary: Defects in autophagy contribute to joint aging and Osteoarthritis (OA). Identifying specific autophagy types could be useful for developing novel treatments for OA.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Cell & Tissue Engineering
M. Pineiro-Ramil, C. Sanjurjo-Rodriguez, S. Rodriguez-Fernandez, T. Hermida-Gomez, F. J. Blanco-Garcia, I. Fuentes-Boquete, C. Vaamonde-Garcia, S. Diaz-Prado
Summary: The aim of this study was to develop immortalized chondrocyte cell lines from articular cartilage of patients with and without osteoarthritis (OA). By transducing primary articular chondrocytes with telomerase reverse transcriptase (hTERT) and SV40 large T antigen (SV40LT), the researchers successfully immortalized the chondrocytes. Although these cell lines were unable to synthesize cartilage-like tissue under the experimental conditions, they retained the ability to respond to IL-113 cytokine, making them advantageous for OA investigation.
BONE & JOINT RESEARCH
(2023)
Article
Cell Biology
Morena Scotece, Carlos Vaamonde-Garcia, Ana Victoria Lechuga-Vieco, Alberto Centeno Cortes, Maria Concepcion Jimenez Gomez, Purificacion Filgueira-Fernandez, Ignacio Rego-Perez, Jose Antonio Enriquez, Francisco J. Blanco
Summary: This study reveals that mtDNA variations have an impact on joint damage during the aging process. By using a conplastic mouse model, it is shown that genetic manipulation of mtDNA can ameliorate joint aging damage, indicating that mtDNA variability is a prognostic factor for aging-related osteoarthritis (OA).