Review
Biotechnology & Applied Microbiology
Robert Ohlendorf, Andreas Moeglich
Summary: Optogenetic regulation of gene expression in bacteria has been achieved by using light-sensitive photoreceptors and genetic circuits. This approach allows precise and reversible control of gene expression in space and time. It has been successfully applied in microbial production, animal stimulation, and biomaterial generation. These applications demonstrate the maturity and potential of optogenetic approaches.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
D. Wu, Y. Weng, Y. Feng, B. Liang, H. Wang, L. Li, Z. Wang
Summary: In periodontitis, Trem1 regulates polarization of M1 macrophages through the STAT3/HIF-1α signaling pathway. Studies suggest that targeting Trem1 could be a potential strategy for the treatment of periodontitis by modulating macrophage polarization.
JOURNAL OF DENTAL RESEARCH
(2022)
Editorial Material
Biology
Xuejing Liu, Zhen Bouman Chen
Summary: Gene expression changes in cultured endothelial cells can be partially reversed by simulating in vivo conditions.
Article
Horticulture
Changxia Li, Xuemei Hou, Kaiping Mou, Huwei Liu, Zongxi Zhao, Weibiao Liao
Summary: This study revealed that glucose and abscisic acid play important roles in adventitious root development in cucumber. Glucose promotes adventitious rooting by enhancing abscisic acid accumulation, while abscisic acid degradation is inhibited by glucose.
SCIENTIA HORTICULTURAE
(2022)
Article
Genetics & Heredity
Tao Jiang, Ruobing Qu, Xuan Liu, Yanjun Hou, Li Wang, Yimin Hua
Summary: This study reveals a novel mechanism contributing to mis-splicing of SMN2 exon 7. The researchers found that hnRNPR interacts with SMN1/2 pre-mRNAs and inhibits exon 7 inclusion.
JOURNAL OF MEDICAL GENETICS
(2023)
Article
Multidisciplinary Sciences
Geoff P. O'Donoghue, Lukasz J. Bugaj, Warren Anderson, Kyle G. Daniels, David J. Rawlings, Wendell A. Lim
Summary: T cells are able to pick out antigenic signals and establish self-tolerance from complex temporal patterns of stimulus. By engineering T cells to respond to light as a stimulus, researchers found that T cells can filter out minute-scale oscillations of activation signal. CD69 expression reached a local minimum at a period of around 25 minutes, suggesting a potential frequency filtering mechanism downstream of the Erk signaling branch. This study highlights the ability of T cell signaling machinery to temporally filter and interpret time-variant input signals in discriminatory ways.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Geoff P. O'Donoghue, Lukasz J. Bugaj, Warren Anderson, Kyle G. Daniels, David J. Rawlings, Wendell A. Lim
Summary: T cells experience complex temporal patterns of stimulation through receptor-ligand-binding interactions with surrounding cells, enabling them to pick out antigenic signals and establish self-tolerance. This study demonstrates that T cells are able to respond to minute-scale oscillations of activation signal and interpret time-variant input signals through a frequency filtering mechanism downstream of the Erk signaling branch. Temporal filtering may play a functional role in vivo, as indicated by observed T cell encounters with self-peptide-presenting antigen-presenting cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Oncology
Cristina Quereda, Angels Pastor, Jose Martin-Nieto
Summary: Dystroglycan (DG) is a glycoprotein that plays a crucial role in maintaining tissue integrity, regulating cellular proliferation, and transmitting extracellular signals to the nucleus. Abnormal glycosylation levels of DG have been found in various human tumors and cancer-derived cell lines, suggesting its potential as a biomarker and therapeutic target for certain types of cancer.
CANCER CELL INTERNATIONAL
(2022)
Article
Immunology
Kogulan Yoganathan, Anqi Yan, Juliana Rocha, Ashton Trotman-Grant, Mahmood Mohtashami, Lisa Wells, Juan Carlos Zuniga-Pfluecker, Michele K. Anderson
Summary: The Alt domain in HEBAlt plays a critical role in integrating cytokine signaling and E protein activity. Phosphorylation of a unique YYY motif in the Alt domain can significantly increase HEBAlt activity, and this increase is dependent on the activity of Janus kinases. HEBAlt is sensitive to changes in cell type and signaling environment during T cell development.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Mechanics
Rati Sharma
Summary: This study investigates the effect of extrinsic noise on mRNA translation and reveals its impact on ribosome residence time, ribosomal traffic, and protein production through stochastic simulations and statistical analysis. The results show that noise in the translation initiation rate significantly affects the distribution of ribosome residence times on mRNA, while the presence of slow codons increases the average residence time.
JOURNAL OF STATISTICAL MECHANICS-THEORY AND EXPERIMENT
(2022)
Article
Multidisciplinary Sciences
Amanda N. Johnson, Guoliang Li, Hossein Jashnsaz, Alexander Thiemicke, Benjamin K. Kesler, Dustin C. Rogers, Gregor Neuert
Summary: Our study shows that signaling is dependent on a rate threshold combined with stimulus concentration to determine the timing of MAPK signaling during rate-varying stimulus treatments. Additionally, we found that the stimulation rate threshold and stimulation rate-dependent cell survival are sensitive to changes in the expression levels of the Ptp2 phosphatase. This suggests that stimulation rate is a regulated determinant of cell behavior and can guide the dissection of major stimulation rate dependent mechanisms in other systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cardiac & Cardiovascular Systems
Islam Osman, Kunzhe Dong, Xiuhua Kang, Luyi Yu, Fei Xu, Abu Shufian Ishtiaq Ahmed, Xiangqin He, Jian Shen, Guoqing Hu, Wei Zhang, Jiliang Zhou
Summary: This study reveals a novel mechanism by which YAP1/TEAD1 promote VSMC proliferation through transcriptional induction of PDGFR beta, thereby enhancing PDGF-BB downstream signaling and promoting neointima formation.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2021)
Article
Cell Biology
Linus Remling, Anke Gregus, Oliver Wirths, Thomas Meyer, Julia Staab
Summary: In this study, we have identified a previously unknown interdimeric interaction site involved in the termination of STAT1 signaling. The introduction of a glutamic acid-to-alanine mutation at position 169 in the coiled-coil domain (CCD) resulted in increased tyrosine phosphorylation, accelerated nuclear import, enhanced DNA-binding affinity, and transcriptional activity compared to the wild-type protein. Furthermore, we demonstrated that the E169 residue in the CCD mediates the release of the dimer from DNA in an autoinhibitory manner.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Multidisciplinary Sciences
Ryan J. Butcher, Jeffrey J. Tabor
Summary: This article mainly introduces a method to monitor the dynamics of RR dimerization in live bacteria in real time through the depolarization of excitation light. Mathematical modeling reveals that TCS phosphosignaling accelerates with SHK expression but decelerates with RR expression and SHK phosphatase activity. Pulsatile activation of the SHK NarX is observed in response to addition and depletion of the extracellular electron acceptor nitrate. The combination of this method with polarized light microscopy enables single-cell measurements of RR dimerization under changing stimulus conditions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Plant Sciences
Y. Khan, Z. Xiong, H. Zhang, S. Liu, T. Yaseen, T. Hui
Summary: The GRAS gene family, derived from GAI, RGA, and SCR, is crucial in plant growth and development regulation and response to environmental stresses. By improving physiological processes, such as hormone regulation and signaling pathways, GRAS enhances plant growth and development, ultimately enabling plants to function more efficiently. Moreover, GRAS plays a significant role in enhancing crop productivity through genetic modification and response to abiotic stresses.
Article
Nutrition & Dietetics
David W. Russ, Cara Acksel, Iva M. Boyd, John Maynard, Katherine W. McCorkle, Neile K. Edens, Sean M. Garvey
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM
(2015)
Article
Geriatrics & Gerontology
D. W. Russ, C. Acksel, K. W. McCorkle, N. K. Edens, S. M. Garvey
JOURNAL OF NUTRITION HEALTH & AGING
(2017)
Article
Geriatrics & Gerontology
Sean M. Garvey, Janis E. Dugle, Adam D. Kennedy, Jonathan E. McDunn, William Kline, Lining Guo, Denis C. Guttridge, Suzette L. Pereira, Neile K. Edens
Article
Geriatrics & Gerontology
David W. Russ, Sean M. Garvey, Clay Densmore, Trevor Hawks, Stephanie Herman, Katherine Pardi
EXPERIMENTAL GERONTOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Jane T. Seto, Monkol Lek, Kate G. R. Quinlan, Peter J. Houweling, Xi F. Zheng, Fleur Garton, Daniel G. MacArthur, Joanna M. Raftery, Sean M. Garvey, Michael A. Hauser, Nan Yang, Stewart I. Head, Kathryn N. North
HUMAN MOLECULAR GENETICS
(2011)
Article
Physiology
Christopher M. Rembold, Sean M. Garvey, Ankit D. Tejani
JOURNAL OF VASCULAR RESEARCH
(2013)
Article
Multidisciplinary Sciences
Julian Vallejo, Madoka Spence, An-Lin Cheng, Leticia Brotto, Neile K. Edens, Sean M. Garvey, Marco Brotto
Article
Biochemistry & Molecular Biology
David W. Russ, Kalina Dimova, Emily Morris, Marguerite Pacheco, Sean M. Garvey, Stylianos P. Scordilis
LIPIDS IN HEALTH AND DISEASE
(2020)
Article
Microbiology
Laura M. Brutscher, Claudia Borgmeier, Sean M. Garvey, Jessica L. Spears
Summary: In this study, the safety of Bacillus subtilis BS50 was assessed through preclinical analyses. The results showed that BS50 genome does not encode any known toxins, is susceptible to commonly prescribed antibiotics, and does not have any negative effects on the viability and permeability of human intestinal epithelial cells. Therefore, BS50 has a preclinical safety profile similar to commercial probiotic strains and poses little risk to human health.
Article
Chemistry, Applied
Sean M. Garvey, Justin L. Guice, Morgan D. Hollins, Caroline H. Best, Kelly M. Tinker
Summary: The objective of this study was to test the hydrolytic efficacy of 6 fungal enzymes in the INFOGEST static in vitro simulation of gastrointestinal (GI) digestion. The study found that these enzymes improved the hydrolysis of dietary protein, lipid, and carbohydrate under simulated gastric or GI conditions, and the combination of these enzymes enhanced macronutrient digestion. The study also validated the efficacy of these enzymes in compromised digestive conditions.
Article
Gastroenterology & Hepatology
Sean M. Garvey, Eunice Mah, Traci M. Blonquist, Valerie N. Kaden, Jessica L. Spears
Summary: This study found that daily supplementation of 2 x 10(9) colony-forming units (CFU) of Bacillus subtilis BS50 is a well-tolerated and safe strategy to alleviate gas-related gastrointestinal symptoms, particularly bloating and burping, in healthy adults. The supplementation did not have any significant negative effects on clinical chemistry, hematology, and intestinal permeability.
Article
Nutrition & Dietetics
Justin L. Guice, Morgan D. Hollins, James G. Farmar, Kelly M. Tinker, Sean M. Garvey
Summary: Fermentable oligo-, di-, monosaccharides and polyols (FODMAPs) have been identified as major contributors to digestive discomfort and intolerances to certain vegetables, fruits, and plant-based foods. This study investigates the use of a food-grade, non-genetically engineered microbial inulinase preparation to hydrolyze fructan-type FODMAPs during gastrointestinal digestion. The results suggest that inulinase supplementation can effectively promote fructan hydrolysis and reduce dietary fructan-type FODMAP exposure.
FRONTIERS IN NUTRITION
(2023)
Article
Microbiology
Sean M. Garvey, Nima K. Emami, Justin L. Guice, Nammalwar Sriranganathan, Christopher Penet, Robert P. Rhoads, Jessica L. Spears, Rami A. Dalloul, Samer W. El-Kadi
Summary: This study aimed to investigate the effects of dietary supplementation of Bacillus subtilis MB40 on the immune system of piglets challenged with the foodborne pathogen Listeria monocytogenes. The results showed that MB40 supplementation reduced the bacterial counts of Listeria in the liver and spleen and decreased the concentration of monocytes in the blood.
Article
Physiology
Sean M. Garvey, David W. Russ, Mary B. Skelding, Janis E. Dugle, Neile K. Edens
PHYSIOLOGICAL REPORTS
(2015)