Article
Ophthalmology
Manpreet Kaur, Sridevi Nair, Saima A. Mazumdar, Jeewan S. Titiyal
Summary: This modified technique of iOCT-guided removal of post-LASIK epithelial ingrowth with interface ethyl alcohol and mitomycin C application effectively prevented recurrence, with real-time visualization helping in decision making and ensuring complete removal of epithelial cell nests for all patients.
INDIAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Developmental Biology
Johanna Englund, Alexandra Ritchie, Leander Blaas, Hanne Cojoc, Nalle Pentinmikko, Julia Dohla, Sharif Iqbal, Manuel Patarroyo, Pekka Katajisto
Summary: This study demonstrates the crucial role of Lama5 in mammary gland development and remodeling, potentially through affecting the Wnt4-mediated signaling pathway between HR+ luminal cells and basal epithelial cells, which in turn impacts gland growth and differentiation.
Article
Engineering, Biomedical
Elrade Rofaani, Yong He, Juan Peng, Yong Chen
Summary: This study developed a method to overcome the lack of in vivo-like basement membrane (BM) for in vitro modeling by culturing primordial lung progenitors (PLPs) on an artificial basement membrane (ABM) composed of collagen IV and laminin. The forces generated by the growing epithelial monolayer on the ABM led to geometry-dependent folding and enhanced expression of surfactant protein C and aquaporin 5 compared with a nanofiber-covered substrate. This work emphasizes the importance of recapitulating natural BM for advanced epithelial modeling.
ACTA BIOMATERIALIA
(2023)
Article
Biotechnology & Applied Microbiology
Cristina Blanco-Elices, Carmen Morales-Alvarez, Jesus Chato-Astrain, Carmen Gonzalez-Gallardo, Paula Avila-Fernandez, Fernando Campos, Ramon Carmona, Miguel Angel Martin-Piedra, Ingrid Garzon, Miguel Alaminos
Summary: The purpose of this study was to determine the potential usefulness of heterotypic cornea substitutes generated with extra-corneal cells in tissue engineering. The stromal layer of these bioartificial corneas showed low levels of components of the tissue extracellular matrix ex vivo, but exhibited higher levels of maturation and spatial organization when grafted in vivo. The results suggest that these bioartificial corneas have the potential for future clinical use.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Ophthalmology
Pranita Sahay, Rahul Kumar Bafna, Jagadesh C. Reddy, Rasik B. Vajpayee, Namrata Sharma
Summary: LASIK surgery is one of the most commonly performed kerato-refractive surgeries globally, known for its safety, efficacy, and predictability. However, rare complications such as flap tear and corneal ectasia may occur. Early identification and appropriate intervention are crucial in achieving optimal visual outcomes even in the presence of complications.
INDIAN JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Andrew Higham, Josiah Dungwa, Natalie Jackson, Dave Singh
Summary: This study investigated the histopathological features of airway remodeling in bronchial biopsies of COPD patients and found relationships between these features and various clinical characteristics.
Article
Ophthalmology
Rodrigo Carlos de Oliveira, George Tye, Lycia Pedral Sampaio, Thomas Michael Shiju, JodiRae DeDreu, A. Sue Menko, Marcony R. Santhiago, Steven E. Wilson
Summary: The study aimed to investigate the expression and localization of TGF beta 1 and TGF beta 2 in rabbit corneas with and without stromal fibrosis, as well as defective perlecan incorporation in the epithelial basement membrane. The findings revealed delayed regeneration of epithelial and EBM barriers in high injury corneas, leading to increased myofibroblast development and stromal fibrosis. Corneas with more severe injuries showed prolonged myofibroblast viability and triggered stromal scarring fibrosis due to delayed regeneration of epithelial and EBM barriers.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Medicine, General & Internal
Aileen Hsieh, Chen Xi Yang, May Al-Fouadi, Kingsley Okechukwu Nwozor, Emmanuel Twumasi Osei, Tillie-Louise Hackett
Summary: In healthy lungs, the connection between the pseudostratified conducting airway epithelium and the reticular basement membrane relies on hemidesmosome junction complexes formed by basal cells and extracellular matrix proteins. However, in patients with asthma, the composition of the basement membrane changes, leading to remodeling characterized by the deposition of different types of collagen. This study determined that fibronectin and collagen-I promote faster attachment and spreading of airway epithelial cells, suggesting a potential protective role of these extracellular matrix proteins in preserving the integrity of the epithelial barrier during airway remodeling in asthma.
FRONTIERS IN MEDICINE
(2023)
Article
Cell Biology
Michael R. Williamson, Cathleen Joy A. Fuertes, Andrew K. Dunn, Michael R. Drew, Theresa A. Jones
Summary: This study demonstrates that reactive astrocytes play a crucial role in vascular repair and remodeling after ischemic stroke in mice, by activating transcriptional programs related to vascular remodeling. Ablation of reactive astrocytes significantly impairs vascular and extracellular matrix remodeling, leading to prolonged blood flow deficits, exacerbated vascular permeability, ongoing cell death, and worsened motor recovery. These findings highlight the critical role of reactive astrocytes as cellular mediators of vascular remodeling during neural repair.
Article
Oncology
Matthia A. Karreman, Alexander T. Bauer, Gergely Solecki, Anna S. Berghoff, Chante D. Mayer, Katharina Frey, Nils Hebach, Manuel J. Feinauer, Nicole L. Schieber, Cedric Tehranian, Luc Mercier, Mahak Singhal, Varun Venkataramani, Marc C. Schubert, Daniel Hinze, Michael Hoelzel, Iris Helfrich, Dirk Schadendorf, Stefan W. Schneider, Dana Westphal, Hellmut G. Augustin, Jacky G. Goetz, Yannick Schwab, Wolfang Wick, Frank Winkler
Summary: Crossing the blood-brain barrier is a crucial step in brain metastasis, and the mechanisms of cancer cell extravasation from brain microcapillaries are not well understood. This study shows that dynamic changes in activated brain microcapillaries promote the initial steps of brain colonization. The inhibition of MMP2/9 and genetic perturbation of MMP9 in cancer cells reduce extravasation and brain metastasis outgrowth. This suggests that the interaction between cancer cells and endothelial cells plays a role in the process of cancer cell extravasation and brain metastasis.
Article
Oncology
Yanze Li, Kai Xu, Ye Zhang, Hu Mao, Qiangmin Qiu, Zhiwei Yan, Xiuheng Liu, Yang Du, Zhiyuan Chen
Summary: In this study, a seven-gene signature related to basement membrane was constructed and validated for accurately predicting the prognosis of bladder urothelial carcinoma (BLCA) patients. The risk score of the signature showed strong correlations with prognosis, immune checkpoint genes, drug sensitivity, and immune cell infiltration landscape. Moreover, experimental results revealed that overexpression of LAMA2, one of the signature genes, inhibited the migration and invasion ability of BLCA cell lines.
Article
Medicine, General & Internal
Majid Moshirfar, Noor F. Basharat, Nour Bundogji, Emilie L. Ungricht, Ines M. Darquea, Matthew E. Conley, Yasmyne C. Ronquillo, Phillip C. Hoopes
Summary: This study evaluated the safety, efficacy, and predictability of laser-assisted in situ keratomileusis (LASIK) enhancement after primary LASIK and compared the outcomes to FDA criteria. The results showed that LASIK enhancements produced favorable visual outcomes and met FDA benchmarks for safety, efficacy, and predictability. Older age, female sex, right eye, and larger sphere, cylinder, and spherical equivalent were identified as risk factors for enhancement. Additionally, the risk of epithelial ingrowth significantly increased when the duration between primary and enhancement procedures exceeded five years.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Ophthalmology
Bhupesh Singh, Sourabh Sharma, Neha Bharti, Dharitri Samantaray, Sudhank Bharti
Summary: This study presents a case of epithelial ingrowth post-LASIK treated with anterior lamellar keratoplasty, which resulted in improved visual acuity and no recurrence after 12 months. Anterior lamellar keratoplasty with femtosecond laser appears to be an effective treatment modality for recurrent epithelial ingrowth after LASIK.
CASE REPORTS IN OPHTHALMOLOGY
(2021)
Article
Biology
D. Pramanik, M. K. Jolly, R. Bhat
Summary: This study used a computational model to investigate the invasion behavior of malignant epithelial tumor cells in different microenvironments, revealing at least four distinct invasive states. These states were found to be specified by different input signatures of proliferation, adhesion, and extracellular matrix remodeling, and specific input perturbations allowed transitions between the clusters, highlighting variations in the robustness between invasive states. This systems-level approach provides quantitative insights into how the diversity in extracellular matrix microenvironments may influence invasion into diverse phenotypic modes during early dissemination of breast cancer, contributing to tumor heterogeneity.
JOURNAL OF THEORETICAL BIOLOGY
(2021)
Article
Ophthalmology
Jose Diaz-Bernal, Ignacio Garcia-Basterra, Jose Mora-Castilla, Annie Nguyen, Yolanda Fernandez-Barrientos, Antonio M. Guerrero
Summary: This study investigated patterns of CET changes after LASIK surgery using AS-OCT and Placido disc-based topography. The results indicated that CET became thicker after surgery independent of preoperative myopia or final refraction, with central and inner ring sectors thickening more than peripheral areas. Clinical variables, including diopters corrected, were not correlated with final CET.
INDIAN JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Ophthalmology
A. Renouvin-Benat, P. Sabatier, F. Varenne, V Pagot-Mathis, P. Fournie, F. Malecaze, A. Gallini, V Soler
JOURNAL FRANCAIS D OPHTALMOLOGIE
(2018)
Article
Ophthalmology
Safa El Hout, Myriam Cassagne, Thomas Sales de Gauzy, Stephane Galiacy, Francois Malecaze, Pierre Fournie
JOURNAL OF CATARACT AND REFRACTIVE SURGERY
(2019)
Review
Ophthalmology
Felix Fremont, Cyrielle Pelissier-Suarez, Pierre Fournie, Marie Porterie, Anais Thevenin, Leonardo Astudillo, Kim Paricaud, Vincent Gualino, Vincent Soler, Gregory Pugnet
Article
Dermatology
A. T. Touhouche, M. Cassagne, E. Berard, F. Giordano-Labadie, A. Didier, P. Fournie, C. Paul, M. Tauber
Summary: This study investigated the incidence and nature of ocular adverse events induced by dupilumab in AD patients. Most patients developed or worsened dry eye disease, with predisposing factors including eyelid eczema and high IgE serum levels. Further research is needed to better understand the pathophysiology of these ocular adverse events.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2021)
Article
Ophthalmology
Gabrielle Lapeyre, Pierre Fournie, Raphael Vernet, Stephanie Roseng, Francois Malecaze, Emmanuelle Bouzigon, David Touboul
Article
Ophthalmology
Alex Matthys, Myriam Cassagne, Stephane D. Galiacy, Safa El Hout, Pierre Fournie, Francois Malecaze
Summary: Transepithelial CXL performed in an oxygen-rich atmosphere results in improved Kmax and CDVA with good safety. The most frequent adverse event was corneal haze.
JOURNAL OF REFRACTIVE SURGERY
(2021)
Article
Multidisciplinary Sciences
Pierre Kantor, Frederic Matonti, Fanny Varenne, Vanessa Sentis, Veronique Pagot-Mathis, Pierre Fournie, Vincent Soler
Summary: The study compared the effectiveness and safety of conventional microscopy and the NGENUITY 3D Visualization System in vitreoretinal surgeries, finding no significant differences in postoperative rates of RD recurrence, MH closure, or decrease in ERM central macular thickness between the two groups. Surgery durations and visual prognosis were also similar. The NGENUITY System shows promise for integration into future robotized interventions.
SCIENTIFIC REPORTS
(2021)
Article
Ophthalmology
Edouard Klein, Francois Malecaze, Victor Bart, Anouk Barnoud, Pierre Levy, Laurent Gauthier, Pierre Fournie
Summary: This study aimed to improve the calculation of intraocular lens power for the second eye in cataract surgery. A new polynomial regression formula (WO2nd) was developed and compared with other formulas. The results showed that WO2nd performed well in predicting refractive errors and was particularly effective for eyes with shorter axial length.
JOURNAL OF CATARACT AND REFRACTIVE SURGERY
(2022)
Review
Allergy
M. Cassagne, S. Galiacy, M. Tauber, C. Paul, P. Fournie, M. Simon
Summary: Atopic dermatitis is a common chronic inflammatory skin disease with multifactorial etiology. Dupilumab, the first approved biomolecule for moderate to severe AD, has shown effectiveness but may cause adverse eye events that require careful management. Understanding molecular mechanisms is key for identifying at-risk patients and optimizing treatment in collaboration between dermatologists and ophthalmologists.
REVUE FRANCAISE D ALLERGOLOGIE
(2021)
Editorial Material
Ophthalmology
F. Varenne, J. Pereira Lopes, C. Escudier, P. Kantor, M. Cassagne, V Soler, P. Fournie
JOURNAL FRANCAIS D OPHTALMOLOGIE
(2022)
Article
Ophthalmology
S. Doan, L. Arnould, C. Febvay, P. Fournie, J. Gueudry, P. Labalette, C. Ouilhon, T-H-C Tran, B. Vabres, S. Barbarot, J-D Bouaziz, A. Du-Thanh, M. Jachiet, J. Seneschal, A. Soria, D. Staumont-Salle, C. Baudouin, B. Mortemousque
Summary: Dupilumab is a monoclonal antibody that inhibits IL4 and IL13 signaling and is used to treat moderate to severe atopic dermatitis. It has been associated with conjunctivitis and blepharoconjunctivitis as side effects, with a higher incidence observed in patients with atopic dermatitis compared to placebo. However, there are currently no official joint recommendations for the prevention and management of conjunctivitis in patients using dupilumab. Further research and guidance are needed.
JOURNAL FRANCAIS D OPHTALMOLOGIE
(2022)
Article
Medicine, General & Internal
Florence Chaudot, Pascal Seve, Antoine Rousseau, Alexandre Thibault Jacques Maria, Pierre Fournie, Pierre Lozach, Jeremy Keraen, Marion Servant, Romain Muller, Baptiste Gramont, Sara Touhami, Habeeb Mahmoud, Pierre-Antoine Quintart, Stephane Dalle, Olivier Lambotte, Laurent Kodjikian, Yvan Jamilloux
Summary: This study analyzed ocular immunotherapy-related adverse events (IRAEs) diagnosed in France after the marketing of immune checkpoint inhibitors (ICPIs). The results showed that these events were more common in patients undergoing treatment for metastatic melanoma, and local or systemic corticosteroids were effective in controlling them.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Ophthalmology
Emmanuelle Loubsens, Raphael Adam, Alexa Debard, Lisa Barioulet, Fanny Varenne, Pierre Fournie, Thomas Sales de Gauzy, Priscille Olle, Guillaume Martin-Blondel, Vincent Soler
Summary: This study reviewed the management, treatment, and outcomes of patients with necrotizing herpetic retinitis (NHR) and proposed an algorithm for first-line management of NHR. The visual prognosis of NHR patients was poor and prompt investigation of immune status and presence of factors justifying intravitreal antiviral injections is necessary.
INTERNATIONAL OPHTHALMOLOGY
(2023)
Article
Multidisciplinary Sciences
Tatiana Lupasco, Zhiguo He, Myriam Cassagne, Tomy Sagnial, Lise Brion, Pierre Fournie, Philippe Gain, Gilles Thuret, Michele Allouche, Francois Malecaze, Michel Simon, Stephane D. Galiacy
Summary: The study found that there is a downregulation of gene expression related to mechanical resistance and oxidative stress pathways in early keratoconus samples. Expression of SPRR2A and HMOX1 is also reduced in advanced keratoconus samples. Additionally, there is a decreased expression of KRT16 and KRT14 proteins, as well as differential localization of involucrin protein. Furthermore, immunofluorescence staining for the active form of NRF2 is reduced in keratoconus epithelia compared to controls.
Article
Allergy
Anna Kychygina, Myriam Cassagne, Marie Tauber, Stephane Galiacy, Carle Paul, Pierre Fournie, Michel Simon
Summary: Dupilumab is a new biological therapy for atopic diseases, which has shown effectiveness in treating atopic dermatitis, asthma, and chronic rhinosinusitis. However, it is associated with various adverse events including injection-site reactions, ophthalmic complications, dermatitis, psoriatic lesions, lymphoma exacerbation, alopecia areata, hypereosinophilia, and arthritis. The molecular origin of these adverse events is unclear and further research is needed.
CLINICAL REVIEWS IN ALLERGY & IMMUNOLOGY
(2022)
