Article
Biotechnology & Applied Microbiology
Jiajia Zhao, Caihong Ma, Yaojie Mei, Jingjing Han, Chen Zhao
Summary: A system for itaconic acid synthesis from cellulose by Neurospora crassa was established, resulting in the highest yield of itaconic acid was 354.08 + 35.99 mg/L. Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis. Enhancing LPMOs-cellobionic acid/gluconic acid system has the potential to reduce energy consumption of the consolidated bioprocessing.
MICROBIAL CELL FACTORIES
(2023)
Article
Microbiology
Ai-Ping Pang, Haiyan Wang, Yongsheng Luo, Zihuayuan Yang, Zhiyu Liu, Zhao Wang, Bingzhi Li, Song Yang, Zhihua Zhou, Xiaolin Lu, Fu-Gen Wu, Zuhong Lu, Fengming Lin
Summary: This study revealed that overexpression of cel3b or cel3g significantly increased extracellular beta-glucosidase production, while overexpression of cel1b inhibited cellulase production. Four cellular distribution patterns of beta-glucosidases in T. reesei were identified, providing insight into their role in cellulase production and cell function. Furthermore, the study elucidated the secretion pathways of different beta-glucosidases, advancing understanding of their properties in filamentous fungi.
Article
Biochemistry & Molecular Biology
Nan-Nan Yu, Wirinthip Ketya, Eun-Ha Choi, Gyungsoon Park
Summary: This study evaluated the potential of a non-thermal atmospheric pressure plasma jet to improve cellulolytic enzyme production efficiency. The results showed that plasma treatment significantly increased the activity and protein concentration of cellulolytic enzymes. Furthermore, plasma treatment promoted the transcription and secretion of cellulolytic enzymes by enhancing the intracellular levels of NO and Ca2+.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ruijie Wang, Manabu Arioka
Summary: Neurospora crassa has multiple xylanases and xylosidases, whose expression is induced by xylan. Deletion of certain enzymes leads to impaired growth on xylan and biomass media, while disruption of specific enzymes results in enhanced xylanase activity in biomass medium. This suggests that xylooligosaccharides released by these enzymes not only serve as carbon sources for N. crassa growth, but also act as inducers for enzyme expression in vivo.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Soil Science
Rachel C. Daughtridge, Yuhei Nakayama, Andrew J. Margenot
Summary: This study evaluated the abiotic hydrolysis of different substrates under various conditions, finding that alkalinization method and substrate type had significant impacts on the extent of abiotic hydrolysis. The study also highlighted the occurrence of appreciable abiotic hydrolysis during the incubation period.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Food Science & Technology
Haiyan Yao, Jianglan Yuan, Rui Chen, Xu Kang, Yuchen Duan, Congxin Lei
Summary: UHPLC-QqQ-MS widely-targeted metabolomics was used to analyze the okara-based metabolites of Neurospora crassa (the strain NC-a and NC-b) at fermented for 24 and 40 h. NC-b exhibited higher cellulase and protease activity than NC-a, especially in the later stage of fermenting okara, and their metabolic profiles between 24 and 40 h were significantly different. A total of 783 metabolites were identified, including 77 key bioactive pharmaceutical ingredients against six major diseases, with flavonoids being the most abundant and diverse. The study demonstrated the chemical basis of fermented okara by NC as functional food ingredients, highlighting the potential for utilizing it as high value-added low-cost materials in food and medicine.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Lingaonan He, Zeyu Duan, Muqun Yu, Shaohua Qi, Ying Wang, Huiqiang Lou, Qun He
Summary: Clearing reactive oxygen species is crucial for the survival of aerobic organisms. This study reveals that the HDA-2-containing complex activates the transcription of the cat-3 gene in the model filamentous fungus Neurospora crassa by facilitating preinitiation complex assembly.
Article
Food Science & Technology
Bradley M. Bartholomai, Katherine M. Ruwe, Jonathan Thurston, Prachi Jha, Kevin Scaife, Ryan Simon, Mohamed Abdelmoteleb, Richard E. Goodman, Moran Farhi
Summary: Neurospora crassa mycoprotein, a whole mycelium food ingredient produced by fermentation, has been proven to be safe and nutritionally valuable. Studies have shown that it has no adverse effects on animal health and no confirmed reports of allergenicity or toxicity in humans. Furthermore, it is rich in complete protein, fiber, potassium, and iron. Overall, foods containing N. crassa can generally be regarded as safe.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Microbiology
Anke Chen, Dan Wang, Rui Ji, Jixi Li, Shaohua Gu, Rong Tang, Chaoneng Ji
Summary: In this study, a thermostable beta-glucosidase (TsBGL) was isolated and characterized from the Thermoprotei Thermofilum sp. ex4484_79, exhibiting high activity and specific activity at elevated temperatures and pH levels. The crystal structure of TsBGL reveals a classical (alpha/beta)(8)-barrel catalytic domain, with catalytic sites including Glu210 and Glu414. TsBGL shows promise for potential industrial applications with its high thermostability and catalytic activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mariana Villalba de la Pena, Pauliina A. M. Summanen, Neda N. Moghadam, Ilkka Kronholm
Summary: In nature, parental effects, where parents pass on information or resources to their offspring, are well documented in plants and animals. However, little is known about parental effects in fungi. In this study, we investigated the presence, duration, adaptive nature, and mechanism of parental effects in the filamentous fungus Neurospora crassa. We found a strong silver-spoon effect in initial mycelium growth, which lasted for one generation. The mechanism of this effect was determined to be spore glycogen content, glucose availability, and a radical transcription shift in spores.
Article
Biotechnology & Applied Microbiology
Tingting Long, Peng Zhang, Jingze Yu, Yushan Gao, Xiaoqin Ran, Yonghao Li
Summary: By adding miglitol to the fermentation medium of T. reesei, the cellulase titer increased more than two-fold. This method has potential in stimulating cellulase production and provides a promising system for T. reesei protein expression.
FERMENTATION-BASEL
(2022)
Article
Plant Sciences
Marino Pedro Reyes-Martin, Emilia Fernandez-Ondono, Irene Ortiz-Bernad, Maria Manuela Abreu
Summary: Agricultural soil quality has been extensively discussed in recent decades. This study focused on three olive grove areas in Portugal with different management techniques, aiming to assess the influence of these techniques on soil quality. The results showed that the concentrations of organic carbon and total nitrogen were highest in the intensive olive grove in Lisbon, while Ca, Mg, Na, and K were the main differences between Lisbon and the other two groves in Santarem. Enzymatic activities were found to be related to various soil concentrations. Soil management and agricultural practices were identified as determining factors for enzymatic activities in the olive groves.
Article
Biochemistry & Molecular Biology
Nan-Nan Yu, Wirinthip Ketya, Gyungsoon Park
Summary: This study investigated the molecular signaling mechanism regulating cellulase production in Neurospora crassa. It was found that intracellular nitric oxide (NO) and cyclic AMP (cAMP) played a role in promoting transcription of cellulolytic enzymes and enhancing extracellular cellulolytic enzyme activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Krisztina Kollath-Leiss, Qin Yang, Hannes Winter, Frank Kempken
Summary: In this study, we confirmed that NcLSP1 is a eisosomal core protein homologous to yeast PIL1 and can be used as an eisosomal marker. We systematically investigated the characteristics of eisosome formation and distribution in different developmental stages by cloning and expressing the nclsp1::trfp reporter gene construct in Neurospora crassa. Additionally, we demonstrated the structural differences on the cellular level between the hyphae germinating from sexual and asexual spores.
Article
Biotechnology & Applied Microbiology
Fanglei Xue, Zhen Zhao, Shuying Gu, Meixin Chen, Jing Xu, Xuegang Luo, Jingen Li, Chaoguang Tian
Summary: This study identified a zinc cysteine transcription factor, Clr-5, which regulates cellulase gene expression in Neurospora crassa by influencing amino acid metabolism. Clr-5 deletion resulted in decreased cellulase activity and downregulation of related genes under Avicel condition. Clr-5 played a crucial role in the utilization of leucine and histidine.