Journal
ARCHIVES OF MICROBIOLOGY
Volume 195, Issue 3, Pages 173-179Publisher
SPRINGER
DOI: 10.1007/s00203-012-0863-5
Keywords
Antibacterial therapy; Cyto-insectotoxin; Chlamydial infection; Chlamydia life cycle; Lachesana tarabaevi
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Funding
- Russian Foundation for Basic Research [08-04-00454, 11-04-00706]
- Ministry of Education and Science of Russian Federation [P1388, 8794, 8282]
- National Association for Innovations and Information Technologies Development [65348]
- Program of Cell and Molecular Biology of the Russian Academy of Sciences
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Antichlamydial activity of cyto-insectotoxin 1a (CIT 1a), representative of a unique class of antimicrobial peptides from the venom of the Central Asian spider Lachesana tarabaevi, was studied. A plasmid vector expressing the cit 1a gene controlled by a human cytomegalovirus tetracycline-dependent promoter was constructed. Impressive inhibition of Chlamydia trachomatis infection in HEK 293 cells transfected by the cit 1a-harboring vector was achieved. With the use of various schemes of cell infection and gene expression induction, it was shown for the first time that an antimicrobial peptide exerts its potent antichlamydial action at an early stage of the pathogen life cycle.
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