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Cell Biology
Xian-liang Zhao, Zhuang-gui Chen, Tian-ci Yang, Ming Jiang, Jie Wang, Zhi-xue Cheng, Man-jun Yang, Jia-xin Zhu, Tian-tuo Zhang, Hui Li, Bo Peng, Xuan-xian Peng
Summary: Exogenous glutamine can enhance the killing effect of antibiotics against multidrug-resistant bacteria, promote the influx of antibiotics, increase intracellular antibiotic concentrations, effectively prevent the development of antibiotic resistance, and provide new insights for the prevention or management of chronic, multidrug-resistant bacterial infections, bacterial persistence, and difficult-to-treat bacterial biofilms.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Agriculture, Dairy & Animal Science
Dandan Fu, Jianmei Wu, Xiaoyan Wu, Ying Shao, Xiangjun Song, Jian Tu, Kezong Qi
Summary: In this study, the researchers found that deletion of the envZ gene in APEC significantly reduced biofilm formation, resistance to stress conditions, and pathogenicity. They also found that EnvZ influenced the adhesion ability of APEC to chicken embryonic fibroblast cells and the expression of inflammatory cytokines. Transcriptome analysis revealed the involvement of outer membrane proteins, stress response systems, and TCSs in the regulatory role of EnvZ. This study provides compelling evidence for the contribution of EnvZ to APEC pathogenicity.
Article
Chemistry, Analytical
Wen Liu, Linbo Wei, Dongmei Wang, Chengye Zhu, Yuting Huang, Zhengjun Gong, Changyu Tang, Meikun Fan
Summary: This study proposes a method to enhance the differentiation of bacterial SERS spectra by applying external environmental stress. The results show that appropriate stress can increase the differences in bacterial SERS spectra.
ANALYTICAL CHEMISTRY
(2022)
Article
Ecology
Estela Ynes Valencia, Jackeline Pinheiro Barros, Thomas Ferenci, Beny Spira
Summary: A trade-off between reproduction and survival is common in many organisms, with Escherichia coli featuring RpoS as a key factor in this balance. Studying strains in a natural environment, it was found that RpoS levels vary widely, leading to a diverse range of stress resistance responses in E. coli strains.
Article
Infectious Diseases
Ryota Ito, Masato Kawamura, Takumi Sato, Shigeru Fujimura
Summary: The acquisition of resistance to Cefmetazole in Escherichia coli can be suppressed by the concomitant use of Relebactam, and the resistance acquisition is associated with decreased expression levels of porin genes.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Multidisciplinary Sciences
Anubhav Tamrakar, Rahul Singh, Amit Kumar, Ravindra D. Makde, Ashish, Prashant Kodgire
Summary: Our study focused on exploring the structure of the HomA and HomB proteins from H. pylori using computational, biophysical, and SAXS techniques. Analysis revealed that HomA/B consist of 8 discontinuous N and C terminal beta-strands forming a small beta-barrel with a large surface-exposed globular domain. Biophysical experiments indicated that HomA and HomB are likely dimeric and that cysteine residues on surface-exposed loops may be involved in protein-protein interactions. This structural information provides insight into the pathogenesis of H. pylori.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Pauline Brepoels, Kenny Appermans, Camilo Andres Perez-Romero, Bram Lories, Kathleen Marchal, Hans P. Steenackers
Summary: This study systematically assessed the effects of the evolved genetic background on resistance evolution against Salmonella Typhimurium, finding that the evolved genetic background contributes significantly to resistance evolution beyond collateral sensitivity. The study also showed that the gradient strength with which cefotaxime was applied affects resistance evolution and adaptation to second antibiotics.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Microbiology
Clemence Cochard, Marine Caby, Peggy Gruau, Edwige Madec, Michael Marceau, Iulia Macavei, Jerome Lemoine, Chrystelle Le Danvic, Franck Bouchart, Brigitte Delrue, Sebastien Bontemps-Gallo, Jean-Marie Lacroix
Summary: This study investigates the importance of gene duplication in host-microorganism interaction, specifically in the emergence of the Dickeya genus. The duplication of the ompF gene has been shown to be deleterious to the virulence of Dickeya dadantii and its expression is not triggered by environmental conditions. These findings have significant implications for understanding the molecular mechanisms of bacterial-plant interactions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Guochao Xu, Lin Xiao, Anning Wu, Ruizhi Han, Ye Ni
Summary: Enhancing microbial tolerance to n-butanol was achieved by overexpressing genes encoding molecular chaperones in E. coli, with the gene rpoS showing the most significant improvement. Double and triple expression of molecular chaperones further optimized n-butanol tolerance of the bacteria, providing guidance for future research in this area.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Ross D. Jones, Yili Qian, Katherine Ilia, Benjamin Wang, Michael T. Laub, Domitilla Del Vecchio, Ron Weiss
Summary: Phosphorylation networks play a crucial role in complex cellular decision making. This study engineers synthetic phosphorylation devices with feedback regulation in mammalian cells and demonstrates their tunable and robust control over cell behaviors. By using bacterial two-component signaling proteins, the authors develop synthetic phosphoregulation devices that can precisely regulate target gene expression and withstand perturbations in mammalian cells. The work lays the foundation for establishing tunable, precise, and robust control over cell behavior with synthetic signaling networks.
NATURE COMMUNICATIONS
(2022)
Article
Veterinary Sciences
Jiangang Hu, Chuanyan Che, Wei Jiang, Zhaoguo Chen, Jian Tu, Xiangan Han, Kezong Qi
Summary: This study found that antibiotic resistance in avian pathogenic Escherichia coli (APEC) is associated with 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (Pfs) rather than with quorum sensing signal molecule AI-2. Pfs affects the sensitivity of APEC to beta-lactam antibiotics by influencing antibiotic-related genes.
VETERINARY SCIENCES
(2022)
Article
Multidisciplinary Sciences
Bram Van den Bergh, Hannah Schramke, Joran Elie Michiels, Tom E. P. Kimkes, Jakub Leszek Radzikowski, Johannes Schimpf, Silke R. Vedelaar, Sabrina Burschel, Liselot Dewachter, Nikola Loncar, Alexander Schmidt, Tim Meijer, Maarten Fauvart, Thorsten Friedrich, Jan Michiels, Matthias Heinemann
Summary: Antibiotic persisters are phenotypic variants within an isogenic bacterial population that are transiently tolerant to antibiotic treatment. This study provides evidence that cytoplasmic acidification, amplified by a compromised respiratory complex I, can act as a signaling hub for perturbed metabolic homeostasis in antibiotic persisters.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Yin Zhai, P. J. Minnick, John P. Pribis, Libertad Garcia-Villada, P. J. Hastings, Christophe Herman, Susan M. Rosenberg
Summary: Antibiotic resistance is a global health threat caused by new mutations. Antibiotics can induce mutations through stress responses, which reveal environmental cues of mutagenesis and are weak links in mutagenesis networks. Inhibiting these networks can slow the evolution of antibiotic resistance.
Article
Microbiology
Yanli Du, Xiangyu Wang, Zongli Han, Ying Hua, Kaina Yan, Bao Zhang, Wei Zhao, Chengsong Wan
Summary: The study demonstrates the essential role of PPK1 in both survival and virulence of EHEC O157:H7, with deletion of ppk1 gene leading to decreased survival rate under heat stress and acid tolerance, as well as reduced adhesion, formation of attaching and effacing lesions, invasion ability, and overall virulence in vivo. These findings suggest that PPK1 is crucial for the pathogenesis of EHEC O157:H7.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Duhyun Ko, Sang Ho Choi
Summary: This study demonstrates that EnvZ/OmpR regulates the expression of OMP genes in Salmonella, thereby enhancing its resistance to antibiotics.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Chemistry, Physical
Nurgun Buyukkidan, Cengiz Yenikaya, Haulil Ilkimen, Ceyda Karahan, Cihan Darcan, Tulin Korkmaz, Yasemin Suzen
JOURNAL OF MOLECULAR STRUCTURE
(2015)
Article
Biology
Onder Idil, Ikbal Macit, Ozge Kaygusuz, Cihan Darcan
ACTA BIOLOGICA HUNGARICA
(2016)
Article
Chemistry, Inorganic & Nuclear
Okan Zafer Yesilel, Bingul Karamahmut, Fatih Semerci, Cihan Darcan, Filiz Yilmaz
JOURNAL OF SOLID STATE CHEMISTRY
(2017)
Article
Biology
C. Darcan, Ebru Aydin
ACTA BIOLOGICA HUNGARICA
(2012)
Article
Chemistry, Multidisciplinary
Fatih Semerci, Okan Zafer Yesilel, Seda Keskin, Cihan Darcan, Murat Tas, Hakan Dal
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Chemistry, Inorganic & Nuclear
Gunes Gunay, Okan Zafer Yesilel, Cihan Darcan, Seda Keskin, Orhan Buyukgungor
INORGANICA CHIMICA ACTA
(2013)
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Chemistry, Physical
Vinod Kumar Gupta, Necip Atar, Mehmet Lutfi Yola, Cihan Darcan, Onder Idil, Zafer Ustundag, Suhas
JOURNAL OF MOLECULAR LIQUIDS
(2013)
Article
Chemistry, Inorganic & Nuclear
Hakan Erer, Okan Zafer Yesilel, Cihan Darcan, Orhan Buyukgungor
Article
Chemistry, Inorganic & Nuclear
Okan Zafer Yesilel, Inci Ilker, Mustafa Serkan Soylu, Cihan Darcan, Yasemin Suzen
Article
Chemistry, Inorganic & Nuclear
N. Buyukkidan, C. Yenikaya, H. Ilkimen, C. Karahan, C. Darcan, E. Sahin
RUSSIAN JOURNAL OF COORDINATION CHEMISTRY
(2013)
Article
Microbiology
Onder Idil, Cihan Darcan, Tevfik Ozen, Resit Ozkanca
JUNDISHAPUR JOURNAL OF MICROBIOLOGY
(2013)
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Biotechnology & Applied Microbiology
Cihan Darcan, Ozge Kaygusuz, Tulin Korkmaz
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
(2013)
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Environmental Sciences
Cihan Darcan, Mustafa Kahyaoglu
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
(2012)
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Biotechnology & Applied Microbiology
Onder Idil, Cihan Darcan, Resit Ozkanca
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
(2011)
