Article
Chemistry, Physical
Megumi Ueda, Seishu Tojo, Tadashi Chosa, Manzo Uchigasaki
Summary: This study investigates the decomposition characteristics of acetate and propionate in a series-type microbial fuel cell (MFC) under different electrode materials, external resistances, and temperatures. The results show that the decomposition rate and maximum power vary with these factors, and the microbial community is significantly affected by temperature.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Energy & Fuels
Kha Lil Dinh, Chin-Tsan Wang, Hue Ngan Dai, Van Man Tran, My Loan Phung Le, Imee A. Saladaga, Yu-An Lin
Summary: The study investigated the impact of different substrate types and concentrations on microbial fuel cells (MFCs), finding that a mixed substrate at a concentration of 30 mM showed the best performance in enhancing power generation and waste removal efficiency.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2021)
Article
Environmental Sciences
Shentan Liu, Zuo Wang, Xiaojuan Feng, Sang-Hyun Pyo
Summary: An innovative design of MER coupled with IAF-PMFC was developed for azo dye wastewater treatment and electricity generation. The sequential degradation of azo dye and the feasibility of energy self-sufficiency were assessed. The combined system achieved high decolorization efficiency and COD removal, even at high dye concentration. The MER/IAF-PMFC technology can simultaneously realize refractory wastewater treatment and balance of electricity production and consumption.
ENVIRONMENTAL RESEARCH
(2023)
Review
Chemistry, Physical
Xiangru Song, ChungHyok Jo, Lujie Han, Minghua Zhou
Summary: This review summarizes recent advances in reactor configuration and coupling technology of MFC in treating antibiotics, analyzing their characteristics in pollutant removal and power output. Comparing removal quantities, the double chamber MFC and membrane-MFC exhibit better performance in removing antibiotics.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Green & Sustainable Science & Technology
Xiayuan Wu, Zixuan Chen, Zuopeng Lv, Lina Zhang, Fengxue Xin, Yan Li, Guannan Liu, Weiliang Dong, Ping Wei, Honghua Jia
Summary: A novel synchronous acclimation strategy involving continuous addition of sludge with chloramphenicol was established to significantly enhance the formation of a highly efficient CAP-degrading anode biofilm in microbial fuel cells. The unique loose and porous biofilm with high permeability and cell viability supported by interwoven cobweb-shaped proteins facilitated mass and electron transfer, primarily leading to the improvements in power density and CAP-tolerant concentration. This study provides a possibility to improve the long-term operation efficiency of antibiotic-degrading electrode biofilms for bioelectrochemical technology through a simple and efficient acclimation strategy.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Green & Sustainable Science & Technology
Sing-Mei Tan, Soon-An Ong, Li-Ngee Ho, Yee-Shian Wong, Che Zulzikrami Azner Abidin, Wei-Eng Thung, Tean -Peng Teoh
Summary: The study developed an integrated configuration for a single chamber microbial fuel cell, enhancing power generation and wastewater treatment performance through specific anodic packing materials and biocathode design. The system achieved high removal rates of COD and NH4+, while also showing improved voltage and power output.
SUSTAINABLE ENERGY TECHNOLOGIES AND ASSESSMENTS
(2021)
Article
Chemistry, Physical
Dingding Ye, Pengqing Zhang, Jun Li, Xun Zhu, Rong Chen, Qiang Liao
Summary: An innovative in situ approach was proposed to visualize biofilm formation in the microchannel for the microfluidic microbial fuel cell (MMFC) anode, which showed that biofilm formed under moderate flow rate exhibited superior bioelectrochemical performance.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Biochemistry & Molecular Biology
Agnieszka A. Pilarska, Anna Marzec-Grzadziel, Emil Paluch, Krzysztof Pilarski, Agnieszka Wolna-Maruwka, Adrianna Kubiak, Tomasz Kaluza, Tomasz Kulupa
Summary: This study conducted anaerobic digestion on confectionery waste using granular polylactide (PLA) as a cell carrier. The results showed that PLA significantly enhanced bacterial proliferation without affecting microbiome biodiversity. The addition of PLA also promoted the growth of biofilm surface area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Xiangyu Han, Jiaman Wang, Jingjing Zhang, Baohong Han, Ning Mei, Runchuan Fan, Jing Zhao, Hong Yao, Xiaohua Yu, Weiwei Cai
Summary: In this study, the role of exDNA in anodic biofilm formation was investigated by digesting exDNA with DNase I enzyme. The results showed that exDNA digestion promotes biofilm formation and enhances anodic coulombic efficiency. Furthermore, the addition of DNase I enzyme improves the abundance of various species but has minimal impact on the relative abundance of exoelectrogens.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Obinna Markraphael Ajunwa, Olubusola Ayoola Odeniyi, Emmanuel Oluwaseun Garuba, Enrico Marsili, Abiodun Anthony Onilude
Summary: Biofilms, flavins, and pyocyanin have been identified as essential metabolites for specific electrogenicity in different species. This study focuses on optimizing biofilm growth and metabolic enhancements to improve bioelectricity production, resulting in enhanced electrogenicity in a consortium of all electrogens exposed to these enhancements.
PROCESS BIOCHEMISTRY
(2021)
Article
Engineering, Environmental
Yuanyuan Zhang, Minmin Jiang, Jinxing Ma, Yuhang Wang, Xingran Zhang, Qiaoyan Wei, Xueye Wang, Xuehong Zhang, Junjian Zheng
Summary: To overcome the limited delivery of active gas to the biofilm on the close-end hollow fiber membranes, a denitrifying H-2-based MBfR was coupled with an automatic venting unit to intermittently update intramembrane gas. The results showed that the periodic venting strategy improved the denitrification performance and increased the biofilm thickness and the presence of denitrifying bacteria and functional genes. The proposed method opens a new avenue for the management of anaerobic MBfR.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Physical
Yan Wang, Kengqiang Zhong, Han Li, Yi Dai, Hongguo Zhang, Jianliang Zuo, Jia Yan, Tangfu Xiao, Xianjie Liu, Yi Lu, Minhua Su, Jinfeng Tang
Summary: CuCo@NCNTs synthesized from copper-based MOFs exhibit excellent ORR performance and antibacterial activity, with a higher power density in MFC compared to Pt/C catalyst. The in-situ grown carbon nanotubes and bimetallic active sites contribute to the enhanced electrocatalytic properties of CuCo@NCNTs.
JOURNAL OF POWER SOURCES
(2021)
Review
Biotechnology & Applied Microbiology
Yujia Jiang, Yansong Liu, Xiaoyu Zhang, Hao Gao, Lu Mou, Mengdi Wu, Wenming Zhang, Fengxue Xin, Min Jiang
Summary: Biofilms can naturally form through microorganisms attaching to supporting materials, providing self-immobilization and high resistance properties. Their application in microbial fermentation process shows promising outlook for future biorefinery platforms, with strategies available for stable biofilm formation through synthetic biology techniques.
BIOTECHNOLOGY ADVANCES
(2021)
Review
Chemistry, Physical
Jianchun Ma, Jun Zhang, Yezhen Zhang, Qilin Guo, Tianjun Hu, He Xiao, Wenbo Lu, Jianfeng Jia
Summary: Microbial fuel cells (MFCs) have gained significant attention worldwide due to their potential for simultaneous wastewater recycling and treatment, as well as bioelectricity generation. Anodic modification materials play a crucial role in improving the efficiency of extracellular electron transfer (EET) between bacteria and the anode, as well as enhancing power density, energy conversion efficiency, and operational durability in MFCs. This paper provides a summary and review of the current application of various anodic modification materials in MFCs, and also discusses future directions for MFC development.
JOURNAL OF POWER SOURCES
(2023)
Article
Chemistry, Physical
Alexiane Godain, Naoufel Haddour, Pascal Fongarland, Timothy M. Vogel
Summary: This study found that under different external resistance conditions, the bacterial community development in the anodic biofilms went through two stages, with the relative abundance of specific electroactive bacteria decreasing with increasing R-ext, and the richness and diversity of the microbial community in the anodic biofilms decreasing with decreasing R-ext.
Article
Biochemistry & Molecular Biology
Stefan Jarl Christensen, Michael Schmidt Madsen, Signe Schram Zinck, Christinne Hedberg, Ole Bandsholm Sorensen, Birte Svensson, Anne S. Meyer
Summary: 4-alpha-glucanotransferase mediated glucan transfer allows for the production of clean label starch ingredients with unique gelling properties. Researchers have studied six different GH77 members and found that four of them exhibit starch modifying activity, leading to a decrease in amylose content, elongation of amylopectin chains, and the formation of firm starch gels. The enzyme Tt4 alpha GT from Thermus thermophilus showed the highest temperature optimum (73 degrees C) and the best potato starch modifying efficacy compared to the other enzymes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Horticulture
I Oliveira, A. Meyer, S. Afonso, B. Goncalves
Summary: This study aimed to compare leaf traits of Portuguese traditional almond cultivars and foreign cultivars under different weather conditions. The results showed the complex behavior of almond trees under drought conditions and highlighted the potential drought resistance in Portuguese cultivars.
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Thore Bach Thomsen, Sune W. Schubert, Cameron J. Hunt, Peter Westh, Anne S. Meyer
Summary: Enzymatic degradation of PET has shown promise as an ecofriendly approach to biodegrading plastic waste. This study presents a novel UV absorbance assay for continuous detection of soluble hydrolysis products released during PET degradation. The assay allows for fast exploration and robust kinetic characterization of PET degrading enzymes.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Lichuang Cao, Cameron J. Hunt, Shang Lin, Anne S. Meyer, Qian Li, Rene Lametsch
Summary: Gamma-Glu-Val-Gly (gamma-EVG) is a potent kokumi peptide that can be synthesized from bovine milk (BoGGT) through the transpeptidase reaction catalyzed by gamma-glutamyl transferase. The molecular mechanism between BoGGT and L-glutamine was explored by generating gamma-glutamyl peptides under various reaction conditions. The suitable conditions for gamma-EVG production using BoGGT were determined, and it was discovered that NaCl could inhibit the transpeptidase activity by disrupting hydrogen bonds between BoGGT and L-glutamine.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Horticulture
Silvia Afonso, Ivo Oliveira, Carlos Ribeiro, Alice Vilela, Anne S. Meyer, Berta Goncalves
Summary: This study aimed to investigate the postharvest effect of plant-based edible coatings on sweet cherries. Results showed that coatings made from extracts of mint, savory and thyme could reduce weight and firmness losses of sweet cherries, and delay their ripening process. These coatings improved the antioxidant activity of sweet cherries without negative effects on their content of bioactive compounds and sensory profile. Therefore, these coatings could be a safe, natural, effective, beneficial and innovative approach to preserve sweet cherry quality in postharvest storage.
SCIENTIA HORTICULTURAE
(2023)
Review
Biotechnology & Applied Microbiology
Jane Wittrup Agger, Michael Schmidt Madsen, Line Korte Martinsen, Pedro Alves Martins, Kristian Barrett, Anne S. Meyer
Summary: This review summarizes the latest research on glucuronoyl esterases (GEs), with a focus on fungal variants. The study reveals that fungal enzymes largely fall into one major branch, with only a minor subset of bacterial enzymes in the same branch. Additionally, some fungal proteins carry a CBM1 binding domain. Molecular docking experiments suggest that there are no direct interactions between the enzyme and lignin polymer, and an elongated carbon chain between the ester linkage and the first phenyl of lignin is preferred. The next step for these enzymes is to explore their application in utilizing lignocellulose as a renewable resource.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Thore B. Thomsen, Sune Schubert, Cameron J. Hunt, Kim Borch, Kenneth Jensen, Jesper Brask, Peter Westh, Anne S. Meyer
Summary: The response of PET-hydrolases to increased substrate crystallinity (X-C) of PET leads to a decrease in reaction rate, but this effect varies for different enzymes. This study examines the influence of X-C on product release rate for six thermostable PET-hydrolases. The results show that the duration of the lag phase before product formation increases with X-C. PHL7 enzyme is efficient on amorphous PET but sensitive to increased X-C, while LCCICCG, LCC, and DuraPETase enzymes tolerate higher X-C and have activity on PET with X-C of 24.4%.
Article
Chemistry, Multidisciplinary
Caio de Oliveira Gorgulho Silva, Marlene Vuillemin, Mirjam A. A. Kabel, Willem J. H. van Berkel, Anne S. S. Meyer, Jane W. W. Agger
Summary: Polyphenol oxidases (PPOs) catalyze the conversion of monophenols to diphenols, which act as reducing agents for lytic polysaccharide monooxygenases (LPMOs). The role of the catalytic products of the PPO MtPPO7 in priming and fueling the activity of the LPMO NcAA9C is investigated. It is found that the catalytic products of MtPPO7 provide the initial electron for the reduction of Cu(II) to Cu(I), but cannot provide sufficient reducing power for continuous fueling of the LPMO activity. This priming reaction occurs with low amounts of MtPPO7 products and does not generate significant levels of H2O2 to fuel the LPMO peroxygenase activity.
Article
Biotechnology & Applied Microbiology
Valentina N. Perna, Sebastian Meier, Anne S. Meyer
Summary: Human milk oligosaccharides (HMOs) are specific glycans in human breast milk that have various beneficial functions for breastfed infants. This study demonstrates the enzymatic production of different types of HMOs in cow's milk through simultaneous enzymatic transglycosylation reactions. The results show that these reactions can be accomplished at low temperature and the products are validated by analytical techniques. This method provides opportunities for enriching milk and infant formulas with HMOs.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Ophthalmology
Philipp Doerschmann, Hubeydullah Akkurt, Georg Kopplin, Maria Dalgaard Mikkelsen, Anne S. Meyer, Johann Roider, Alexa Klettner
Summary: Age-related macular degeneration (AMD) is a major cause of severe vision loss. This study established gene expression panels for inflammation, oxidative stress, and angiogenesis in porcine retinal pigment epithelium (RPE), and investigated the effects of fucoidans on gene expression. The results showed that fucoidans can reduce the impact of inflammation, oxidative stress, and angiogenesis in AMD.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Shang Lin, Cameron J. Hunt, Jesper Holck, Jesper Brask, Kristian B. R. M. Krogh, Anne S. Meyer, Casper Wilkens, Jane W. Agger
Summary: The study found that esterases from various bacteria and fungi can catalyze the release of diferulic acids from complex arabinoxylans. By comparing the activity of the enzymes on synthetic substrates and diFA release, a detailed structure-function analysis is needed to explain the diFA releasing ability of the enzymes. The findings contribute to a better understanding of the role of esterases in the degradation of complex arabinoxylans and provide new opportunities for enzyme-assisted upgrading of complex bran arabinoxylans.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Environmental Sciences
Karolina Bachosz, Jakub Zdarta, Muhammad Bilal, Anne S. Meyer, Teofil Jesionowski
Summary: Enzymatic processes are becoming increasingly important, and the role of enzymatic cofactors is crucial for efficient biocatalytic activities. Therefore, the development of cofactor regeneration systems is essential. This review summarizes current knowledge on enzymatic regeneration methods and highlights potential directions for further development.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Thore Bach Thomsen, Tobias S. Radmer, Anne S. Meyer
Summary: Plastic pollution, particularly from PET, is a significant environmental challenge. This study investigates the enzyme kinetics of PET degradation and finds that the degree of crystallinity (X-C) has a substantial impact on the enzymatic reaction rate. The mobile amorphous fraction (X-MAF) is a better descriptor for the rate of enzymatic degradation. The longer lag phase observed with increased X-C is proposed to be due to increased substrate entanglement density and unproductive enzyme binding to the crystalline regions of PET.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Sonia Mohamadnia, Anders Thygesen, Parisa Ghofrani-Isfahani, Anna Pia Monachese, Borja Valverde-Perez, Irini Angelidaki
Summary: Wastewater from the potato processing industry, protamylasse, can be valorized through the cultivation of microalgae by mixotrophic metabolism. A new two-stage system was developed, where volatile fatty acids (VFA) were produced through anaerobic acidification (AA), and Chlorella sorokiniana was cultivated for valorization of nutrients and organics. This process resulted in high removal efficiencies of phosphorus, nitrate, and ammonia, and the production of microalgal biomass with high protein content.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biochemical Research Methods
Maria Dalgaard Mikkelsen, Vy Ha Nguyen Tran, Sebastian Meier, Thuan Thi Nguyen, Jesper Holck, Hang Thi Thuy Cao, Tran Thi Thanh Van, Pham Duc Thinh, Anne S. Meyer, Jens Preben Morth
Summary: This study presents the structural characterization and activity of a novel endo-alpha(1,4)-fucoidanase, Mef1, from the marine bacterium Muricauda eckloniae. Mef1 specifically catalyzes the cleavage of alpha(1,4)-linkages between fucose residues in fucoidan from Fucus evanescens. The crystal structure of Mef1 reveals a single-domain organization with an extended, positively charged groove as the active site.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2023)
