Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 99, Issue 7, Pages 3191-3200Publisher
SPRINGER
DOI: 10.1007/s00253-014-6294-2
Keywords
Lactobacillus buchneri; Gamma-aminobutyric acid; Xylose; Corncob hydrolysate
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Funding
- National Key Basic Research Program of China (973 Program) [2012CB725202]
- National Natural Science Foundation of China [NSFC31370131]
- Six Talent Peaks Project of Jiangsu Province [2012-SWYY-008]
- Jiangnan University [JUSRP51303A]
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Lactobacillus buchneri strain WPZ001 that could efficiently produce gamma-aminobutyric acid was isolated from Chinese fermented sausages. Optimal cultivation conditions for gamma-aminobutyric acid production in L. buchneri WPZ001 were determined, and xylose was found to be the best carbon source. Using xylose as the sole carbon source, 70 g/L gamma-aminobutyric acid was produced by flask fermentation of L. buchneri WPZ001 for 48 h, and the harvested cells could continue to convert monosodium glutamate to gamma-aminobutyric acid in buffer and produce 59 g gamma-aminobutyric acid after eight runs of biotransformation; the total yield of gamma-aminobutyric acid reached 129 g/L. This combination strategy also worked well when the low-cost corncob hydrolysate was used as the sole carbon source, and the yield of gamma-aminobutyric acid reached 117 g/L. The results indicate that L. buchneri WPZ001 has great potential for industrial production of gamma-aminobutyric acid.
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