Article
Biotechnology & Applied Microbiology
Ana Karen Malan, Thalita Tuleski, Ana Ines Catalan, Emanuel Maltempi de Souza, Silvia Batista
Summary: Herbaspirillum seropedicae is a beta-proteobacterium capable of utilizing d-xylose as a carbon source. Through RNA-seq analysis and targeted mutant studies, it was found that H. seropedicae Z69 utilizes non-phosphorylative pathways to catabolize d-xylose, involving co-expression of two routes: the Weimberg pathway and a novel pathway that synthesizes pyruvate and glycolate. This novel pathway appears to play a significant role in d-xylose metabolism.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Karen R. Jonscher, Winyoo Chowanadisai, Robert B. Rucker
Summary: Pyrroloquinoline quinone (PQQ) is associated with biological processes such as mitochondriogenesis, reproduction, growth, and aging, and can attenuate clinically relevant dysfunctions related to ischemia, inflammation, and lipotoxicity. As an enzymatic cofactor or accessory factor, PQQ modulates lactate acid and other dehydrogenases to enhance NAD+-dependent sirtuin activity and mediate mitochondrial functions.
Article
Biochemistry & Molecular Biology
Thuy Minh Nguyen, Kotone Naoki, Naoya Kataoka, Minenosuke Matsutani, Yoshitaka Ano, Osao Adachi, Kazunobu Matsushita, Toshiharu Yakushi
Summary: This study characterized the PQQ-DH9 of Gluconobacter sp. strain CHM43, showing its high oxidation activity towards secondary alcohols but lower activity on linear substrates compared to GLDH. The experimental results of gene expression suggest the important role of PQQ-DH9 in the cell membrane.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Engineering, Chemical
Chongrong Ke, Yang Ren, Ping Gao, Jian Han, Yong Tao, Jianzhong Huang, Xinwei Yang
Summary: This study developed a two-step process for the large-scale separation and purification of PQQ from crude fermentation broth, achieving high purity PQQ crystals. The process involved pretreatment, resin purification, and standardized operational parameters for scaled-up PQQ purification.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Kui Wang, Xiao-Yan Wang, Guo-Jie Gao, Mi-Ni Wang, Ying-Ying Yu, Siyang Xing, Bolin Zhu
Summary: A pH-triggered transition from micellar aggregation to a host-guest complex was achieved by supramolecular interactions between calixpyridinium and pyrroloquinoline quinone disodium salt (PQQ-2Na), accompanied by a color change. This transition was further studied as a controllable-release model due to the low toxicity of the involved compounds.
Review
Biochemistry & Molecular Biology
Nur Syafiqah Mohamad Ishak, Kazuto Ikemoto
Summary: Obesity is a global health concern with increasing prevalence. Pyrroloquinoline quinone (PQQ), found naturally in some foods and available as a dietary supplement, has potential health benefits due to its antioxidant and anti-inflammatory properties. PQQ influences mitochondrial function, which is associated with obesity complications.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
S. A. Masudul Hoque, Takashi Umehara, Tomoko Kawai, Masayuki Shimada
Summary: This study elucidated the negative effects of ROS on mitochondrial functions in FSH-stimulated granulosa cells during follicular development, and investigated the potential of PQQ treatment to ameliorate these effects. PQQ treatment reduced ROS levels and oxidative stress, improved cell viability and function, and increased the number of ovulated oocytes and pups per delivery.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Microbiology
Ying Xiao, Kaijuan Wu, Syeda Sundas Batool, Qingqun Wang, Hao Chen, Xingyu Zhai, Zheng Yu, Jing Huang
Summary: This study reports a calcium (Ca2+)-dependent alcohol dehydrogenase PedE_M.s. derived from a methylotroph Methylopila sp. M107, which was heterologously expressed in the host Methylorubrum extorquens AM1. The enzyme exhibited high catalytic activity and affinity for ethanol as a substrate, and its activity could be enhanced by potassium (K+) and calcium (Ca2+). Furthermore, the optimal temperature and pH of PedE_M.s. were found to be 30°C and pH 9.0, respectively. The enzymatic properties of PedE_M.s. contribute to our understanding of the methanol/ethanol family PQQ-dependent dehydrogenase.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
ShaoPei Li, Aruna Raja, Meissam Noroozifar, Kagan Kerman
Summary: Parkinson's disease (PD) is associated with the aggregation and misfolding of α-synuclein (α-syn) protein. A proof-of-concept study found that pyrroloquinoline quinone (PQQ) can effectively prevent ROS formation and fibrillation of α-syn-119 in the presence of Cu(II), suggesting PQQ as a potential multitarget therapeutic agent for PD.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Oncology
Shumei Gao, Qiao Zhou, Hui Jin, Naiqi Shi, Xiaoyu Wang, Li Zhang, Meijuan Yan
Summary: The study found that PQQ reduces apoptosis levels and increases autophagy levels in LPS-induced HAPI microglial cells by regulating the PI3K/Akt signaling pathway. Additionally, PQQ alters the distribution of LAMP2 in the cytoplasm and nucleus, impacting the expression of TNFR1 and TNFR2. These results suggest that PQQ may have neuroprotective effects on microglial cells through these mechanisms.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Neurosciences
Alessio Canovai, James R. Tribble, Melissa Joe, Daniela Y. Westerlund, Rosario Amato, Ian A. Trounce, Massimo Dal Monte, Pete A. Williams
Summary: Retinal ganglion cells require regulated metabolism and functional mitochondria to maintain ATP levels and cell viability. Alterations in metabolism and mitochondrial processes are early indicators of optic neuropathies. PQQ, a quinone cofactor, has potential neuroprotective effects and can affect cellular and mitochondrial metabolism, but its mechanisms need to be further studied.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Article
Oncology
Qiao Zhou, Hui Jin, Naiqi Shi, Shumei Gao, Xiaoyu Wang, Shunxing Zhu, Meijuan Yan
Summary: These results indicate that PQQ can improve motor function in hind limbs and the pathological changes of neurons and injured spinal cord after SCI, reduce the expressions of IL-10, TNF-alpha, and IL-6, inhibit apoptosis after SCI, as well as inhibit LPS-induced apoptosis and inflammation of astrocytes.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Samiksha Joshi, Saurabh Gangola, Vandana Jaggi, Manvika Sahgal
Summary: In this study, functionally active phosphate solubilizing bacteria (PSB) associated with Shisham plant were screened and 18 bacteria capable of solubilizing tri-calcium phosphate were identified. All 18 isolates showed zinc solubilization, indole acetic acid (IAA), siderophore, and hydrogen cyanide (HCN) production. Phylogenetic analysis identified the bacterial strains as belonging to Pseudomonas, Klebsiella, Streptomyces, Pantoea, Kitasatospora, Micrococcus, and Staphylococcus genera. One isolate, L4 from Lacchiwala region, showed the highest efficiency in phosphate solubilization. The selected PSB strains have potential applications in plant growth promotion, disease suppression, antioxidant activity, and yield enhancement.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Alexa M. Schmitz, Brooke Pian, Sean Medin, Matthew C. Reid, Mingming Wu, Esteban Gazel, Buz Barstow
Summary: Researchers developed a whole-genome knockout collection of mutants for Gluconobacter oxydans, identifying genes influencing the efficacy of rare earth element bioleaching. Disruption of genes related to Pyrroloquinoline Quinone synthesis and glucose dehydrogenase significantly reduces bioleaching, while disruption of phosphate-specific transport system genes enhances bioleaching efficiency. This study offers a roadmap for engineering G. oxydans' genome to improve bioleaching efficiency.
NATURE COMMUNICATIONS
(2021)
Review
Biochemical Research Methods
Jan L. Kruesemann, Vittorio Rainaldi, Charles A. R. Cotton, Nico J. Claassens, Steffen N. Lindner
Summary: Methanol is a promising feedstock for industrial bioproduction due to its renewable production, high solubility, and limited microbial toxicity. The first enzymatic oxidation step to formaldehyde is a key challenge for its bio-industrial application. Different types of methanol dehydrogenases (MDH) can catalyze this reaction using NAD+, O2, or PQQ as an electron acceptor. NAD-dependent MDH have high energetic efficiency but mediocre kinetics, while O2-dependent methanol oxidases require high oxygen concentrations and do not conserve energy. PQQ-dependent MDH provide a good compromise between energy efficiency and kinetics, making them a promising solution for industry.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Purvi Zaveri, Nasreen Munshi, Alok Vaidya, Sanjay Jha, G. Naresh Kumar
CANADIAN JOURNAL OF MICROBIOLOGY
(2015)
Article
Microbiology
Sumeet Pandey, Ashish Singh, Nirja Chaudhari, Laxmipriya P. Nampoothiri, G. Naresh Kumar
CURRENT MICROBIOLOGY
(2015)
Article
Geriatrics & Gerontology
Ashish Kumar Singh, Sumeet Kumar Pandey, Gourav Saha, Naresh Kumar Gattupalli
EXPERIMENTAL GERONTOLOGY
(2015)
Article
Agronomy
Jitendra Wagh, Kumar Chanchal, Shah Sonal, Bhandari Praveena, G. Archana, G. Naresh Kumar
Article
Biochemical Research Methods
Ujwal Trivedi, Shubham Kaushik, Prashant Kunjadia, Matheshwaran Saravanan, Valakunja Nagaraja, Gattupalli Archana, Gattupalli Nareshkumar
PROTEIN EXPRESSION AND PURIFICATION
(2016)
Article
Substance Abuse
Ashish K. Singh, Sumeet K. Pandey, Gattupalli Naresh Kumar
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2014)
Article
Biotechnology & Applied Microbiology
Kavita Yadav, Chanchal Kumar, G. Archana, G. Naresh Kumar
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2014)
Article
Environmental Sciences
Raina Jain, Sanjay Jha, Hemanta Adhikary, Prasant Kumar, Vipul Parekh, Anamika Jha, Mahesh K. Mahatma, G. Naresh Kumar
GEOMICROBIOLOGY JOURNAL
(2014)
Article
Microbiology
Rahul Jog, Maharshi Pandya, G. Nareshkumar, Shalini Rajkumar
Article
Agronomy
Jitendra Wagh, Praveena Bhandari, Sonal Shah, G. Archana, G. Naresh Kumar
Article
Multidisciplinary Sciences
Kavita Yadav, Chanchal Kumar, G. Archana, G. Naresh Kumar
Article
Multidisciplinary Sciences
Hemanta Adhikary, Paulomi B. Sanghavi, Silviya R. Macwan, Gattupalli Archana, G. Naresh Kumar
Article
Multidisciplinary Sciences
Mahendrapal Singh Rajput, Bhagya Iyer, Maharshi Pandya, Rahul Jog, Naresh G. Kumar, Shalini Rajkumar
Article
Biotechnology & Applied Microbiology
Prasant Kumar, Ayush V. Ranawade, Naresh G. Kumar
BIOMED RESEARCH INTERNATIONAL
(2014)
Article
Biotechnology & Applied Microbiology
Riddhi Vyas, Maharshi Pandya, Jayashree Pohnerkar, G. Naresh Kumar