Review
Biotechnology & Applied Microbiology
Priscila Esteves Faria, Aline M. Castro, Denise Maria Guimaraes Freire, Rafael D. Mesquita
Summary: This article elucidates the details of isomer production of 2,3-butanediol and acetoin, non-oxidative metabolism products of microorganisms, through bioinformatics analysis and compares the stereo-triads of other enzymes. These results are of great importance for understanding the biosynthesis and industrial production of BD/AC.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Yuchen Li, Xiangying Zhao, Mingjing Yao, Wenli Yang, Yanlei Han, Liping Liu, Jiaxiang Zhang, Jianjun Liu
Summary: This paper reviews the synthesis pathways and formation mechanisms of different stereoisomers of 3-hydroxybutanone (acetoin) and 2,3-butanediol in microorganisms. It also summarizes the characteristics and affinities of different types of butanediol dehydrogenases (BDH) and discusses the recent research on producing optically pure acetoin or 2,3-butanediol using microorganisms. The limiting factors and possible solutions for chiral acetoin and 2,3-butanediol production are also discussed.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Sang-Jeong Bae, Sujin Kim, Hyun June Park, Joonwon Kim, Hyunbin Jin, Byung-gee Kim, Ji-Sook Hahn
Summary: The study achieved an increase in acetoin production through adaptive laboratory evolution and elimination of byproducts. By identifying Ora1, Ypr1, and Ara1 as contributors to byproduct formation, further optimization of (R)-acetoin production was achieved.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Kaloyan Petrov, Penka Petrova
Summary: The review discusses the advantages of Bacillus spp. as potential producers of acetoin and 2,3-BD, including their non-pathogenic nature, adaptability to various substrates, and the potential for genetic engineering and process optimization. While Bacillus spp. are among the best acetoin producers, they also synthesize 2,3-BD comparable to pathogenic producers but with relatively lower productivity, which could be improved through future research.
FERMENTATION-BASEL
(2021)
Review
Biotechnology & Applied Microbiology
Sofia Maina, Ashish A. Prabhu, Narisetty Vivek, Anestis Vlysidis, Apostolis Koutinas, Vinod Kumar
Summary: This review summarizes the applications of bio-based platform chemicals 2,3-butanediol (BDO) and acetoin in various industries, as well as the novel technological developments in genetic and metabolic engineering strategies for their production. The review also discusses the valorization of renewable feedstocks and the development of bioprocesses for the production of BDO and acetoin. Techno-economic aspects evaluating the viability and industrial potential of bio-based BDO production are presented.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Cell Biology
Wensi Meng, Lijie Zhang, Menghao Cao, Yongjia Zhang, Yipeng Zhang, Ping Li, Zhaoqi Kang, Shiting Guo, Ping Xu, Cuiqing Ma, Chao Gao
Summary: Research demonstrates that acetate accumulation in certain bacterial growth processes exhibits an initial increase followed by a decrease. The coupling strategy involving 2,3-BD biosynthesis and ethanol production is widely distributed in bacteria and crucial for the elimination of toxic acetate. This unique overflow metabolism with desirable metabolic functions is important for industrial applications of various bacteria.
Article
Biotechnology & Applied Microbiology
Lin Shi, Yuan Lin, Jiaao Song, Hongxing Li, Yinhao Gao, Yonghong Lin, Xiaowen Huang, Wu Meng, Weishuai Qin
Summary: In this study, a recombinant plasmid with dual promoter was constructed to overexpress 2,3-butanediol dehydrogenase in Bacillus subtilis BS2. The optimized fermentation conditions resulted in a higher production of 2,3-butanediol, acetoin and tetramethylpyrazine. Additionally, a photocatalytic coenzyme regeneration system was introduced to further enhance the metabolic yield of 2,3-butanediol.
FERMENTATION-BASEL
(2023)
Article
Chemistry, Analytical
Melanie Welden, Robin Severins, Arshak Poghossian, Christina Wege, Johannes Bongaerts, Petra Siegert, Michael Keusgen, Michael J. Schoning
Summary: This study presents a novel sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl in alcoholic beverages. The sensor exhibits high sensitivity and long-term stability, and enables the successive detection of acetoin and diacetyl by tuning the pH value of the measurement solution.
Article
Biotechnology & Applied Microbiology
Lili Sheng, Abubakar Madika, Matthew S. H. Lau, Ying Zhang, Nigel P. Minton
Summary: This study engineered the thermophile Parageobacillus thermoglucosidasius NCIMB 11955 to produce 3-hydroxybutanone and 2,3-butanediol, organic compounds with commercial applications. By minimizing by-product formation and addressing redox imbalance, the production of targeted metabolites was significantly improved. The highest titre of 2,3-butanediol produced in Parageobacillus and Geobacillus species to date, 15.6 g/L, was achieved in media supplemented with 5% glucose.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wanggang Tang, Chaoqun Lian, Yu Si, Jianrong Chang
Summary: The NAD(H)-dependent (2R,3R)-BDH from the pathogen Neisseria gonorrhoeae exhibits stereospecific oxidation and reduction activity, providing insights into 2,3-BD metabolism in this pathogen and its potential impact on virulence.
MOLECULAR BIOTECHNOLOGY
(2021)
Article
Microbiology
Zachary J. Butz, Alexander Hendricks, Kanda Borgognoni, Christopher J. Ackerson
Summary: A Rhodococcus erythropolis bacterium was identified to tolerate normally lethal concentrations of various metal ions, with a mycothione reductase (Mtr) showing remarkable selectivity for TeO32- reduction. The selectivity for TeO32- is attributed to aromatic residues near the active site of the enzyme, resulting in a Te-0 enzymatic product resembling Te nanowires.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaofei Wang, Lingyun Jia, Fangling Ji
Summary: In this study, the X-ray crystal structure of Bacillus subtilis R,R-BDH was successfully crystallized and solved. A zinc ion was found in the catalytic center, coordinated by Cys37, His70 and Glu152, stabilizing the chiral substrates observed in the predicted molecular docking model. The interaction patterns of different chiral substrates in the molecular docking model explained the react priority measured by the enzyme activity assay of R,R-BDH. Site-directed mutation experiments determined the importance of amino acids Cys37, Thr244, Ile268, and Lys340 in the catalytic active center. The structural information presented in this study enhances the understanding of the catalytic mechanism of BDHs and provides useful guidance for directional engineering of R,R-BDH for high-purity monochiral BD and AC.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2023)
Article
Agricultural Engineering
Sofia Maina, Roland Schneider, Maria Alexandri, Harris Papapostolou, George-John Nychas, Apostolis Koutinas, Joachim Venus
Summary: The production of acetoin and D-BDO by Bacillus amyloliquefaciens cultivated on bakery waste hydrolysates can be influenced by varying the volumetric oxygen transfer coefficient (kLa). Batch fermentations with different kLa values were found to have significant impact on the production yields and rates, with fed-batch cultures at higher kLa values leading to combined production of acetoin, meso-BDO and D-BDO. The results suggest that manipulating the kLa value can redirect bacterial metabolism towards higher acetoin or D-BDO production during fermentation in crude bakery waste hydrolysates.
BIORESOURCE TECHNOLOGY
(2021)
Article
Energy & Fuels
Anne Lunze, Benedikt Heyman, Yosra Chammakhi, Maria Eichhorn, Jochen Buechs, Nico Anders, Antje C. Spiess
Summary: The study successfully demonstrated the technical suitability of Silphium perfoliatum as a feedstock for a liquid hot water-based biorefinery process through experimental comparison and analysis. It also showed the potential application value of Silphium perfoliatum in the biorefinery process.
BIOENERGY RESEARCH
(2021)
Article
Chemistry, Physical
Yaping Wang, Yanhong Peng, Xiaoyan Liu, Ronghua Zhou, Xianqing Liao, Yong Min, Yong Hu, Ying Wang, Ben Rao
Summary: An auto-inducing expression system was developed to express target genes in S. marcescens MG1, creating a whole-cell biocatalyst for 2,3-butanediol and acetoin production. Enzymes were co-expressed to build NADH regeneration systems for transforming diacetyl to 2,3-butanediol and acetoin respectively, showcasing high productivities and activities in the engineered strains. This study demonstrates the potential of using S. marcescens as an effective whole-cell biocatalyst for bio-based chemical production.
Article
Biochemistry & Molecular Biology
Jian-yong Zheng, Yin-yan Liu, Wei-feng Luo, Ren-chao Zheng, Xiang-xian Ying, Zhao Wang
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2016)
Article
Biochemical Research Methods
Jian-yong Zheng, Jian Wang, Sha-sha Zhou, Xiao-jun Li, Xiang-xian Ying, Zhao Wang
PROTEIN EXPRESSION AND PURIFICATION
(2017)
Article
Plant Sciences
Man Zhao, Xiangxian Ying, Jie Sun, Zhao Wang
JOURNAL OF SYSTEMATICS AND EVOLUTION
(2017)
Article
Biochemistry & Molecular Biology
Man Zhao, Peng Chen, Wenyi Wang, Fengjie Yuan, Danhua Zhu, Zhao Wang, Xiangxian Ying
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Spectroscopy
Xing Liu, Xiangxian Ying, Yanli Li, Hua Yang, Wanshan Hao, Meilan Yu
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2018)
Article
Biochemistry & Molecular Biology
Xiangxian Ying, Jie Zhang, Can Wang, Meijuan Huang, Yuting Ji, Feng Cheng, Meilan Yu, Zhao Wang, Meirong Ying
Article
Multidisciplinary Sciences
Man Zhao, Wenyi Wang, Lei Wei, Peng Chen, Fengjie Yuan, Zhao Wang, Xiangxian Ying
Article
Biochemistry & Molecular Biology
Xiangxian Ying, Shihua Yu, Meijuan Huang, Ran Wei, Shumin Meng, Feng Cheng, Meilan Yu, Meirong Ying, Man Zhao, Zhao Wang
Article
Chemistry, Physical
Xiangxian Ying, Can Wang, Shuai Shao, Qizhou Wang, Xueting Zhou, Yanbing Bai, Liang Chen, Chenze Lu, Man Zhao, Zhao Wang
Review
Biochemistry & Molecular Biology
Lianjie Ye, Larwubah Kollie, Xing Liu, Wei Guo, Xiangxian Ying, Jun Zhu, Shengjie Yang, Meilan Yu
Summary: The development of novel nanoparticles as a new generation therapeutic drug platform is an active field of chemistry and cancer research. Fullerene nanoparticles have unique physical and chemical properties, excellent biocompatibility, and significant anti-tumor activity. Understanding the anti-tumor mechanism of fullerene nanoparticles is crucial for designing anti-tumor drugs with low toxicity and high targeting.
Article
Chemistry, Physical
Yunpeng Jia, Qizhou Wang, Jingjing Qiao, Binbin Feng, Xueting Zhou, Lijun Jin, Yingting Feng, Duxia Yang, Chenze Lu, Xiangxian Ying
Summary: A novel approach for the one-pot double reduction of (E/Z)-citral to (S)-citronellol was achieved in a multi-enzymatic cascade system, using N-ethylmaleimide reductase, alcohol dehydrogenase, and a variant of glucose dehydrogenase. The engineered E. coli strain co-expressing the enzymes displayed high conversion rates and enantioselectivity, demonstrating potential for industrial application.
Article
Chemistry, Physical
Wei Guo, Xing Liu, Lianjie Ye, Jie Liu, Kollie Larwubah, Ge Meng, Weiqiang Shen, Xiangxian Ying, Jun Zhu, Shengjie Yang, Jianjun Guo, Yanrong Jia, Meilan Yu
Summary: Hydroxylated fullerene C-60(OH)(n) (n = 18-22) has both growth-promoting and inhibitory effects on leukemia cells, with high concentrations leading to cell death.
Article
Chemistry, Physical
Binbin Feng, Xia Li, Lijun Jin, Yi Wang, Yi Tang, Yuhao Hua, Chenze Lu, Jie Sun, Yinjun Zhang, Xiangxian Ying
Summary: In this study, the old yellow enzyme was improved through protein engineering, resulting in increased activity and strict (S)-enantioselectivity. The modified enzyme showed higher catalytic efficiency in the reduction of (E/Z)-citral compared to the wild type. Furthermore, co-expression of the modified enzyme with alcohol dehydrogenase and glucose dehydrogenase shortened the reaction time and increased the product concentration.
Article
Chemistry, Multidisciplinary
Jingjing Qiao, Duxia Yang, Yingting Feng, Wan Wei, Xun Liu, Yinjun Zhang, Jianyong Zheng, Xiangxian Ying
Summary: Esterase/lipase-catalyzed selective hydrolysis of d,l-menthyl esters is a promising method for producing l-menthol. In this study, a highly active para-nitrobenzyl esterase from Bacillus subtilis 168 was cloned and engineered to enhance its l-enantioselectivity. The substitution of Ala400 to Pro significantly improved the E value. The developed methodology achieved high conversion, strict l-enantioselectivity, and green process.
Article
Biotechnology & Applied Microbiology
Man Zhao, Liang Gao, Li Zhang, Yanbin Bai, Liang Chen, Meilan Yu, Feng Cheng, Jie Sun, Zhao Wang, Xiangxian Ying
BIOTECHNOLOGY LETTERS
(2017)