Article
Virology
Cui Lin, Jingyu Hu, Yadong Dai, Huandong Zhang, Kang Xu, Weiren Dong, Yan Yan, Xiran Peng, Jiyong Zhou, Jinyan Gu
Summary: Circovirus is the smallest virus to cause immune suppression in pigs. The nuclear entry and stability of the capsid protein (Cap) of porcine circovirus type 2 (PCV2) are crucial for its infection and replication. IPO5 acts as a host factor that maintains self-stability of PCV2 virions and promotes their nuclear import. By directly binding to Cap, IPO5 blocks proteasome degradation and facilitates PCV2 replication.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Dedong Wang, Lei Hou, Ying Ji, Jiali Xie, Jie Zhao, Ning Zhu, Xiaoyu Yang, Jianwei Zhou, Yongqiu Cui, Jinshuo Guo, Xufei Feng, Jue Liu
Summary: Porcine circovirus type 3 (PCV3) is a virus that causes a wide range of illnesses in pigs. This study revealed that PCV3 capsid protein interacts with nucleolin and degrades it, requiring the recruitment of the enzyme RNF34. Nucleolin degradation by PCV3 capsid protein occurs in the nucleolus. Additionally, nucleolin decreases PCV3 replication by promoting the release of interferon beta. These findings provide important insights into the mechanism by which nucleolin modulates PCV3 replication and offer a potential strategy for preventing and controlling PCV3 infection.
JOURNAL OF VIROLOGY
(2023)
Article
Chemistry, Multidisciplinary
Supaporn Klangprapan, Chang-Ching Weng, Wan-Ting Huang, Yaw-Kuen Li, Kiattawee Choowongkomon
Summary: Porcine circovirus type 2 (PCV2) is the main cause of porcine circovirus-associated disease (PCVAD), resulting in significant economic losses for the swine industry globally. In this study, single-chain variable fragments (scFvs) were selected and characterized against the PCV2 capsid using phage display technology. This research demonstrates the potential of scFvs as receptors for biosensor devices in the detection of PCV2 capsid.
Article
Virology
Xin Ma, Changjie Lv, Qianqian Wang, Chen Li, Peixin Wang, Chen Luo, Yifan Wu, Tingting Wei, Siying Liu, Fathalrhman Eisa Addoma Adam, Zengqi Yang, Xinglong Wang
Summary: This study demonstrated that C1QBP interacts with the Cap NLS region, resulting in changes in the intracellular localisation of the Cap protein. Overexpression of C1QBP inhibits the proliferation of PCV2, possibly related to the function of C1QBP in controlling nuclear transport of Cap.
ARCHIVES OF VIROLOGY
(2021)
Article
Immunology
Nicholas J. MacDonald, Kavita Singh, Karine Reiter, Nguyen Vu, Richard Shimp, Apostolos G. Gittis, Beth Chen, Martin Burkhardt, Baoshan Zhang, Zhixiong Wang, Raul Herrera, Mackenzie Moler, Duck-Yeon Lee, Sachy Orr-Gonzalez, Jessica Herrod, Lynn E. Lambert, Kelly M. Rausch, Olga Muratova, David S. Jones, Yimin Wu, Albert J. Jin, David N. Garboczi, Patrick E. Duffy, David L. Narum
Summary: The development of a malaria vaccine that can block transmission of the parasite from mosquitoes to humans is crucial for malaria elimination efforts. The use of Pfs25, a protein on the surface of the parasite, is being investigated as a transmission-blocking vaccine. Two recombinant forms of Pfs25, Pfs25H and Pfs25M, have been studied for their structural and immunological differences.
Article
Microbiology
Zhenyu Wang, Jing Chen, Qiong-ge Zhang, Kai Huang, Dan Ma, Qian Du, Dewen Tong, Yong Huang
Summary: PCV2 infection interferes with the activation of type I interferon signaling pathway through its Cap protein and host gC1qR protein, resulting in reduced transcription of IFN-stimulated genes.
VETERINARY MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Elvira Tarasova, Noriaki Okimoto, Shanshan Feng, Dmitry Nerukh, Reza Khayat, Makoto Taiji
Summary: The study found that pure PCV2 capsid proteins require acidic conditions to assemble into empty capsids in vitro in the absence of nucleic acids. Molecular dynamics simulation revealed that an appropriate protonation configuration is necessary for driving capsid assembly, with assembly being prohibited at neutral pH due to electrostatic repulsion.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Microbiology
Lun Yao, Yufang Cheng, Hao Wu, Ahmed H. Ghonaim, Shengxian Fan, Wentao Li, Qigai He
Summary: Porcine circovirus-associated diseases and pseudorabies are highly contagious and economically significant diseases of swine in China. The study identifies a promising vaccine candidate for porcine pseudorabies virus and porcine circovirus type 3, providing immunoprotection against these diseases.
VETERINARY MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Qingping Luo, Junqi Zhou, Weihua Tang, Pei Jiang, Xun Wan, Waqas Ahmed, Ali Mohsin, Yingping Zhuang, Meijin Guo
Summary: This study investigated and optimized the expression process of PCV2 capsid protein in HEK293F cells. It was found that the PCV2 capsid gene affected several genes involved in protein folding, stress response, and translation process in HEK293F cells. By using a combined strategy of protein engineering and VPA addition, the production of engineered PCV2 capsid protein in HEK293F cells was significantly increased.
PROTEIN EXPRESSION AND PURIFICATION
(2023)
Article
Veterinary Sciences
Libin Wen, Jiaping Zhu, Fengxi Zhang, Qi Xiao, Jianping Xie, Kongwang He
Summary: This study identified interactions between the Cap protein of porcine circovirus-like virus P1 and five cellular proteins, including Ral GDS, which was confirmed by co-immunoprecipitation. These findings support future investigations into the mechanisms of P1 infection and pathogenesis.
BMC VETERINARY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Weilong Ji, Xinwei Zhang, Guyu Niu, Si Chen, Xue Li, Lin Yang, Liying Zhang, Linzhu Ren
Summary: This study demonstrates that recombinant capsids of PCV can serve as candidate antigens for vaccine development, as they exhibit adequate immunogenicity and can induce specific antibodies. Additionally, the antibodies prepared in this study have the potential for cross-reactivity with multiple PCVs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Chia-Chun Chang, Ching-Ying Wu, Jhao-Guan Ciou, Ching-Wei Wu, Yi-Chen Wang, Hui-Wen Chang, Maw-Sheng Chien, Chienjin Huang
Summary: This study generated a monoclonal antibody specific to the capsid protein of PCV3, which can be used for diagnosis and pathogenesis studies. A novel linear B-cell epitope and a potential nuclear localization signal in the capsid protein were identified. Recombinant proteins self-assembled into virus-like particles (VLPs) with correct conformation and specificity, providing insights for the development of diagnostic tools and potential vaccines for PCV3.
Article
Microbiology
Jianwei Zhou, Yuexia Wang, Yonghui Qiu, Yongxia Wang, Xiaoyu Yang, Changzhe Liu, Yongyan Shi, Xufei Feng, Lei Hou, Jue Liu
Summary: The study found that PCV4 Cap mediated the translocation of DDX21 from the nucleolus to the cytoplasm and confirmed that the NoLS of PCV4 Cap directly bound to DDX21, demonstrating for the first time that DDX21 binds directly to the NoLS of the PCV4 Cap and contributes to its nucleolar localization.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Wanting Yu, Yuao Sun, Qing He, Chaoying Sun, Tian Dong, Luhua Zhang, Yang Zhan, Naidong Wang, Yi Yang, Yujie Sun
Summary: The mitochondrial localization signal (MLS) in the PCV2 Cap is critical for Cap-induced apoptosis and essential for PCV2 replication, as deletion of the MLS is lethal for virus rescue.
VETERINARY SCIENCES
(2021)
Article
Veterinary Sciences
Shuang Li, Bo Wang, Shun Jiang, Xiaohui Lan, Yongbo Qiao, Jiaojiao Nie, Yuhe Yin, Yuhua Shi, Wei Kong, Yaming Shan
Summary: In this study, a rAAV8 vector was constructed to express PCV2 Cap, resulting in strong cellular and humoral immune responses in vitro and in mice. The results suggest that rAAV8 vector could serve as a potential vaccine candidate against PCV2.
JOURNAL OF VETERINARY SCIENCE
(2021)