Article
Fisheries
Nathaniel A. Sibinga, Helene Marquis
Summary: This study investigated the latent infection of Yersinia ruckeri in rainbow trout, finding that qPCR detection of the bacteria in spleen DNA is a preferred standard for carrier detection. The research highlights the importance of effective monitoring for subclinical infections in aquaculture for proactive disease management.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Carla Fernandez-Espinel, Marco Medina-Morillo, Rute Irgang, Giovanna Sotil, Henry Araya-Leon, Violeta Flores-Dominick, Jesus L. Romalde, Ruben Avendano-Herrera, Jefferson Yunis-Aguinaga
Summary: This study aimed to identify and classify Yersinia ruckeri isolates from the Puno Region in Peru. Two biotypes were found among the isolates, but they shared identical fingerprints and serotypes. This information is important for the epidemiological research and vaccine development against Y. ruckeri.
JOURNAL OF FISH DISEASES
(2023)
Article
Environmental Sciences
Elham Alsadat Banihashemi, Siyavash Soltanian, Amin Gholamhosseini, Mahdi Banaee
Summary: This study suggests that exposure to MPs and simultaneous challenge with Y. ruckeri could synergistically affect clinical parameters in fish.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Di Wang, Simeng Sun, Shaowu Li, Tongyan Lu, Dongfang Shi
Summary: This study conducted transcriptomic profiling of spleen samples from rainbow trout at 24 h post-Y. ruckeri infection and identified 2498 differentially expressed genes related to immune response pathways. The findings provide insight into the molecular mechanisms of rainbow trout immune responses to Y. ruckeri infection.
Article
Fisheries
Tao Liu, Zheng-Yang Zhou, Chen-wang Lu, Shang-Jie Bai, Jun Wang, Gao-Xue Wang
Summary: In this study, the researchers found that the T4SS of Yersinia ruckeri SC09 mediated bacterial virulence and immune evasion, possibly through the secretion of molecules such as STIR-1, STIR-2, and STIR-3. These findings contribute to a better understanding of the general rules of bacterial pathogenicity.
Article
Agriculture, Dairy & Animal Science
Joanna Pajdak-Czaus, Patrycja Schulz, Elibieta Terech-Majewska, Wojciech Szweda, Andrzej Krzysztof Siwicki, Aleksandra Platt-Samoraj
Summary: The study found that IPNV infection may lead to secondary bacterial infections, affecting non-specific immunity. The influence of pathogens on non-specific immunity depends on the time between infections.
Article
Fisheries
Ezgi Dincturk, Tevfik Tansel Tanrikul
Summary: The study revealed a co-infection of Yersinia ruckeri and Pseudomonas fluorescens in rainbow trout farms in Mugla, Turkey, causing significant mortalities and pathological signs. The pathogens were identified through biochemical and molecular tests, showing resistance to various antibiotics. Infection experiments indicated high mortality rates and pathogenicity of both Y. ruckeri and P. fluorescens.
AQUACULTURE RESEARCH
(2021)
Article
Fisheries
Ertugrul Terzi, Bunyamin Kucukkosker, Soner Bilen, Osman Nezih Kenanoglu, Orhan Corum, Mehmet Ozbek, Sukru Senol Parug
Summary: The aqueous methanolic extract of thin-skinned plum showed positive effects on the growth, immune response, and resistance to a pathogenic bacterium in rainbow trout. Specifically, it improved innate immunity, survival against Yersinia ruckeri, and decreased the feed conversion ratio level significantly.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Veterinary Sciences
Antoine Rostang, Carole Peroz, Catherine Fournel, Chantal Thorin, Segolene Calvez
Summary: The use of enrofloxacin in trout farms for the treatment of yersiniosis should be reevaluated, as a single intraperitoneal injection of ENR at 10 mg/kg appears to be more effective than repeated oral administrations. This study suggests the need for revising the oral doses used in trout farms.
Article
Fisheries
Soner Bilen, Selcuk Ispir, Osman Nezih Kenanoglu, Yigit Tastan, Kerim Guney, Ertugrul Terzi
Summary: The study explored the effects of Greek juniper extract on immune responses of rainbow trout, showing that the extract had various impacts such as increasing lysozyme activity, decreasing myeloperoxidase activity, and up-regulating interleukins. Additionally, the juniper extract demonstrated a protective effect against Yersinia ruckeri, leading to a higher survival rate in rainbow trout treated with it.
JOURNAL OF FISH DISEASES
(2021)
Article
Microbiology
Mostafa Y. Abdel-Glil, Uwe Fischer, Dieter Steinhagen, Una McCarthy, Heinrich Neubauer, Lisa D. Sprague
Summary: The study conducted a comparative analysis of Yersinia ruckeri whole genome sequences, revealing their genetic relatedness to other members of the genus and potential genes and mechanisms involved in pathogenicity pathways. It provides new insights into the taxonomy and evolution of Y. ruckeri strains, offering a framework for more efficient control strategies for this pathogen.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Takamitsu Sakai, Yukihiro Nakagawa, Chihaya Nakayasu
Summary: This study characterized a strain (NBRC102019) isolated from river sediment and 9 isolates from a disease outbreak. The serotype of NBRC102019 was classified as O-6, while the serotypes of the 9 isolates from the outbreak were all O-7. Multilocus sequence typing revealed that the 9 isolates belonged to the same genotype, distinct from NBRC102019. Challenge tests showed that the outbreak isolates caused substantial mortality in chum salmon, while NBRC102019 did not, indicating a distinct lineage for the Y. ruckeri causing the outbreak.
Article
Biochemistry & Molecular Biology
Carlos Fajardo, Paulo Santos, Ricardo Passos, Mariana Vaz, Rita Azeredo, Marina Machado, Sergio Fernandez-Boo, Teresa Baptista, Benjamin Costas
Summary: This study evaluated the short-term innate immune response of rainbow trout to infection with Y. ruckeri. The results showed significant decreases in hematological parameters and oxidative stress indicators in the infected group compared to the control group. There were also significant differences in gene expression between infected individuals and the control group.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Wenjie Xu, Mingyang Fu, Manhong Huang, Xiaowen Cui, Yi Li, Min Cao, Libin Wang, Xiaohui Xiong, Xiong Xiong
Summary: A novel method for rapid identification of S. solar and O. mykiss based on duplex PCR assay was developed successfully in this study. Specific primers design and melting curve analysis enabled accurate discrimination of the two fish species, offering a feasible solution for detecting adulteration in processed fish products.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Veterinary Sciences
Filiz Ozcan, Neval Berrin Arserim, Muzaffer Aydin Ketani, Kasim Aydin, Nurdan Karacan-Sever, Nida Ozcan, Burcak Aslan-Celik
Summary: This study aimed to detect Yersinia ruckeri bacteria in fish samples collected from rainbow trout farms in the South Eastern Anatolia Region of Turkey. The bacteria were found in the liver, kidney, and muscle tissue samples of fish from 23 out of 40 farms. These findings emphasize the need for preventive measures, such as vaccination and reducing stress factors, in these farms.
REVISTA CIENTIFICA-FACULTAD DE CIENCIAS VETERINARIAS
(2023)
Correction
Virology
Enrique Rivadulla, Jesus L. Romalde
Article
Engineering, Environmental
Liguan Li, Joseph Nesme, Marcos Quintela-Baluja, Sabela Balboa, Syed Hashsham, Maggie R. Williams, Zhuofeng Yu, Soren J. Sorensen, David W. Graham, Jesus L. Romalde, Arnaud Dechesne, Barth F. Smets
Summary: The study of urban wastewater systems in Denmark, Spain, and the UK revealed a significant reduction in antibiotic resistance genes during sewage conveyance, presenting opportunities for antibiotic resistance management and caution for surveillance based on sewage.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Review
Infectious Diseases
David Polo, Alberto Lema, Enia Gandara, Jesus L. Romalde
Summary: Through a systematic review and meta-analysis of 35 studies in Europe, it was found that HBoV has a moderate prevalence among European countries, with no significant differences in prevalence by country. The study also analyzed the prevalence of HBoV as a single infection or co-infection with other viruses.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Microbiology
Chunqi Jiang, Mami Tanaka, Sayo Nishikawa, Sayaka Mino, Jesus L. Romalde, Fabiano L. Thompson, Bruno Gomez-Gil, Tomoo Sawabe
Summary: This study presents the second update of molecular phylogenetic analysis of Vibrionaceae, adding new insights into the diversity and evolutionary history of this bacterial family. The results show that the classification based on nucleotide sequences of eight housekeeping genes is reliable, and the set of 11 ribosomal protein genes is useful for identifying uncultured species among metagenome-assembled genome pools. This research contributes to taxonomic applications in classifying not-yet-cultured taxa and expanding our understanding of Vibrionaceae.
CURRENT MICROBIOLOGY
(2022)
Article
Virology
Maria Soderlund-Venermo, Anupam Varma, Deyin Guo, Douglas P. Gladue, Emma Poole, Flor H. Pujol, Hanu Pappu, Jesus L. Romalde, Laura Kramer, Mariana Baz, Marietjie Venter, Matthew D. Moore, Michael M. Nevels, Sayeh Ezzikouri, Vikram N. Vakharia, William C. Wilson, Yashpal S. Malik, Zhengli Shi, Ahmed S. Abdel-Moneim
Summary: This article summarizes the presentations given at the 1st international conference of the World Society for Virology (WSV). The conference focused on tackling global viral epidemics and aimed to foster international collaborations. The conference consisted of multiple sessions and attracted 270 scientists from 49 countries.
Article
Food Science & Technology
Soren Saxmose Nielsen, Dominique Joseph Bicout, Paolo Calistri, Elisabetta Canali, Julian Ashley Drewe, Bruno Garin-Bastuji, Jose Luis Gonzales Rojas, Christian Gortazar Schmidt, Mette Herskin, Virginie Michel, Miguel Angel Miranda Chueca, Barbara Padalino, Paolo Pasquali, Helen Clare Roberts, Hans Spoolder, Karl Stahl, Antonio Velarde, Arvo Viltrop, Christoph Winckler, Jeroen Dewulf, Luca Guardabassi, Friederike Hilbert, Rodolphe Mader, Jesus L. Romalde, Peter Smith, Francesca Baldinelli, Lisa Kohnle, Julio Alvarez
Summary: This article evaluates antimicrobial-resistant bacteria that pose a threat to the health of kept fish species and provides an overview of the global status of resistance in certain bacteria. Based on limited scientific evidence, no specific bacteria were identified as the most relevant antimicrobial-resistant species in the EU's kept fish species.
Article
Microbiology
Pablo Santibanez, Jesus Romalde, Derie Fuentes, Antonio Figueras, Jaime Figueroa
Summary: This study focused on the health status of Mytilus chilensis in Chile, revealing a good health status with no significant lesions or presence of mandatory notification pathogens. The importance of an integrated vision of different disciplines for the sustainability of the mussel industry in Chile was highlighted.
Article
Marine & Freshwater Biology
Diego Gerpe, Aide Lasa, Alberto Lema, Sabela Balboa, Jesus L. Romalde
Summary: The study of the microbiota associated with clams is important for understanding their health status and preventing pathobiology events. This study used various microbiological techniques to analyze two clam species in northwest Spain, revealing a rich bacterial diversity and isolating new bacterial taxa.
Article
Microbiology
Chunqi Jiang, Hisae Kasai, Sayaka Mino, Jesus L. Romalde, Tomoo Sawabe
Summary: In this study, the complete genome sequences of all type strains in the Splendidus clade were obtained and analyzed. The results showed that these strains have different genome sizes and plasmid numbers, which may be related to their environmental adaptation and pathogenicity. Furthermore, a broad prediction of virulence factors revealed core and species unique VF genes in the Splendidus clade.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Marcos Quintela-Baluja, Kelly Jobling, David W. Graham, Mariano Gomez-Lopez, Jesus L. Romalde, Jorge Barros-Velazquez, Pilar Calo-Mata
Summary: Raoultella ornithinolytica, an increasingly important pathogen in human diseases, was isolated from the effluent of a domestic wastewater treatment plant in Spain. The nearly complete genome sequence of a multidrug-resistant strain, R. ornithinolytica MQB_Silv_108, was reported. Its release into the environment poses a possible exposure risk for humans and animals.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Fisheries
Muhammed Duman, Noemi Bujan, Soner Altun, Jesus L. Romalde, Izzet Burcin Saticioglu
Summary: This study characterized Vibrio isolates from Turkish fish farms, PubMLST database, and GenBank using multilocus sequence analysis and typing. They found high diversity and genetic interactions within the Vibrio population. 17 new sequence types were discovered, indicating frequent genetic recombination. These findings help understand differences in Vibrio infections in fish and provide insights for effective measures in aquaculture.
Article
Fisheries
Carla Fernandez-Espinel, Marco Medina-Morillo, Rute Irgang, Giovanna Sotil, Henry Araya-Leon, Violeta Flores-Dominick, Jesus L. Romalde, Ruben Avendano-Herrera, Jefferson Yunis-Aguinaga
Summary: This study aimed to identify and classify Yersinia ruckeri isolates from the Puno Region in Peru. Two biotypes were found among the isolates, but they shared identical fingerprints and serotypes. This information is important for the epidemiological research and vaccine development against Y. ruckeri.
JOURNAL OF FISH DISEASES
(2023)
Article
Multidisciplinary Sciences
Ryota Yamano, Juanwen Yu, Alfabetian Harjuno Condro Haditomo, Chunqi Jiang, Sayaka Mino, Jesus L. Romalde, Kyuhee Kang, Yuichi Sakai, Tomoo Sawabe
Summary: This study isolated a Gram-negative, aerobic bacterium named PTE2(T) from laboratory-reared Japanese sea cucumber larvae. Phylogenetic analysis revealed that PTE2(T) was closely related to Thalassotalea sediminis N211(T), but had some differences, indicating the novelty of this strain. Based on genome-based taxonomic approaches, PTE2(T) is proposed as a new species, Thalassotalea hakodatensis sp. nov.
Article
Multidisciplinary Sciences
Rika Kudo, Ryota Yamano, Juanwen Yu, Shotaro Koike, Alfabetian Harjuno Condro Haditomo, Mayanne A. M. de Freitas, Jiro Tsuchiya, Sayaka Mino, Fabiano Thompson, Jesus L. Romalde, Hisae Kasai, Yuichi Sakai, Tomoo Sawabe
Summary: Strain PT1(T), a rod-shaped bacterium isolated from laboratory-reared larvae of sea cucumber Apostichopus japonicus, demonstrated similarities to Neptuniibacter marinus ATR 1.1(T) and Neptuniibacter caesariensis MED92(T) with sequence similarities of 98.2% and 98.1%, respectively, based on the analysis of 16S rRNA gene nucleotide sequences. However, the average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values among these three strains were below 95% and 70%, confirming the novel status of PT1(T). Based on the genomic approach, a new species, Neptuniibacter victor sp. nov., is proposed for PT1(T).
Article
Fisheries
Jose R. Lopez, Jesus L. Romalde, Montserrat Lopez-Sanmartin, Jose I. Navas
Summary: In this study, a disease outbreak in meagre caused by Vibrio tapetis was investigated. The mortality rate reached 45% within one week. The bacteria were recovered from the liver and kidney of the affected fish, and further characterization confirmed their pathogenicity in sea bass.
