Article
Biotechnology & Applied Microbiology
Sujit Sadashiv Jagtap, Anshu Deewan, Jing-Jing Liu, Hanna E. Walukiewicz, Eun Ju Yun, Yong-Su Jin, Christopher V. Rao
Summary: This study investigated the central carbon metabolism in Rhodosporidium toruloides during growth on different substrates using transcriptomics and metabolomics. Significant changes in gene expression and metabolite concentrations were observed, with a notable finding in xylose metabolism. These results enhance understanding of R. toruloides metabolism and may guide metabolic engineering and model development for this organism.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shiyu Liang, Yue Zhang, Liting Lyu, Shuang Wang, Zongbao K. Zhao
Summary: A recombinant beta-1,3-glucomannanase, MAN5C, was introduced into the genome of the red yeast Rhodosporidium toruloides, enabling the secretion of active MAN5C and simplifying the lipid extraction process, resulting in a higher lipid recovery rate.
BIORESOURCES AND BIOPROCESSING
(2023)
Article
Agricultural Engineering
Zhijia Liu, Marlene Fels, Giuliano Dragone, Solange Mussatto
Summary: This study investigated the effects of biomass-derived inhibitors on the growth of oleaginous yeast Rhodosporidium toruloides, with furfural identified as the most potent inhibitor. An evolved strain demonstrated improved ability to tolerate toxic compounds, indicating adaptive laboratory evolution as a potential strategy for obtaining oleaginous yeasts with enhanced growth capabilities in biomass hydrolysates.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Energy & Fuels
Senthilnathan Sri Laxma Alankar, Nithianandam Sajesh, Shrestha Rastogi, Simar Sakhuja, Gunasekaran Rajeswari, Vinod Kumar, Anuj Kumar Chandel, Samuel Jacob
Summary: This study utilized microwave-acid pretreatment on water hyacinth to produce liquid hydrolysate for oleaginous yeast fermentation, with detoxification and comparison of non-detoxified hydrolysate efficiency. Results showed higher sugar concentration in non-detoxified hydrolysate, which when supplemented with yeast extract, produced maximum lipid yield and single cell protein content. Kinetic models and GC-MS analysis confirmed the efficiency of hydrolysate supplemented media, demonstrating its potential in biodiesel production within a sustainable framework.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Biotechnology & Applied Microbiology
Liting Lyu, Yadong Chu, Sufang Zhang, Yue Zhang, Qitian Huang, Shuang Wang, Zongbao K. Zhao
Summary: Researchers have developed engineered strains of Rhodosporidium toruloides by overexpressing genes responsible for phenolic compound degradation, leading to improved resistance to typical phenolic inhibitors and increased lipid production and cell growth in concentrated mode biomass hydrolysates.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Fengzhu Ling, Xin Tang, Hao Zhang, Yong Q. Chen, Jianxin Zhao, Haiqin Chen, Wei Chen
Summary: Overexpression of MaYHM in Mortierella alpina resulted in increased fatty acid content and altered metabolic flux, providing more carbon for fatty acid synthesis. This study sheds new light on improving industrial lipid production in M. alpina.
BIOTECHNOLOGY LETTERS
(2021)
Article
Microbiology
Xiang Jiao, Liting Lyu, Yue Zhang, Qitian Huang, Renhui Zhou, Shian Wang, Shuang Wang, Sufang Zhang, Zongbao K. Zhao
Summary: This study successfully reduced lipid production in Rhodosporidium toruloides NP11 by knocking out two lipid droplet structural proteins, providing valuable information for studying microbial lipid metabolism and constructing advanced cell factories.
FEMS MICROBIOLOGY LETTERS
(2021)
Article
Nutrition & Dietetics
Xin Tang, Xiaoqi Sun, Xuxu Wang, Hao Zhang, Yong Q. Chen, Jianxin Zhao, Haiqin Chen, Wei Chen
Summary: Mortierella alpina has a strong capacity for lipid accumulation. Isocitrate dehydrogenase (IDH) plays a crucial role in affecting intracellular carbon sources and reducing power NADPH for lipid biosynthesis. In this study, overexpression of NAD (+)-specific MaIDH3 reduced total lipid content, while overexpression of NADP (+)-specific MaIDH4 enhanced total fatty acid content. This research provides insights into the mechanisms of IDH function and the potential for increasing lipid production in M. alpina.
FRONTIERS IN NUTRITION
(2021)
Article
Agricultural Engineering
Xuehai Zheng, Rongfei Hu, Duo Chen, Jiannan Chen, Wenjin He, Luqiang Huang, Chentao Lin, Huibin Chen, Youqiang Chen, Jinmao Zhu, Feng Qi, Ting Xue
Summary: The addition of cane molasses at concentrations of 35 g/L and 70 g/L significantly increased lipid and carotenoid production in the microalgae R. toruloides M18, demonstrating a cost-effective and environmentally friendly strategy for enhancing production of these valuable compounds.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Paul A. Adamczyk, Samuel T. Coradetti, John M. Gladden
Summary: R. toruloides is a highly tolerant oleaginous yeast with diverse metabolic capacities. This study presents a refined and extended model of R. toruloides pentose metabolism based on genetic and metabolite data. The identified enzymes responsible for pathway flux provide a better understanding of the complexity of microbial pentose metabolism.
MICROBIAL CELL FACTORIES
(2023)
Article
Environmental Sciences
Sangeeta Singh, Tanmay Bharadwaj, Devendra Verma, Kasturi Dutta
Summary: The study found that the oleaginous yeast Rhodosporidium toruloides 9564(T) can degrade phenol and produce lipids. Increasing the inoculum size can overcome the toxicity of phenol. The results indicate that using industrial wastewater for bioenergy production can promote the circular economy.
Article
Biotechnology & Applied Microbiology
Feixiang Liu, Zewei Lu, Tingting Lu, Manman Shi, Huimin Wang, Rong Wu, Jun Cao, Erzheng Su, Xiaoqiang Ma
Summary: Insufficient biosynthesis efficiency during the lipogenic phase is a major obstacle to overproducing very long-chain fatty acids. This study successfully engineered an oleaginous yeast to overproduce nervonic acid by improving the biosynthesis of very long-chain fatty acids during the lipogenic phase. By evaluating different ketoacyl-CoA synthases and conducting a genome-wide transcriptional analysis, the researchers were able to optimize the biosynthetic pathway and overcome the bottleneck of fatty acid accumulation. The engineered strain achieved high levels of nervonic acid production.
METABOLIC ENGINEERING
(2023)
Article
Agricultural Engineering
Carlos S. Osorio-Gonzalez, Rahul Saini, Krishnamoorthy Hegde, Satinder K. Brar, Antonio Avalos Ramirez
Summary: This study observed the ability of Rhodosporidium toruloides-1588 to grow and accumulate lipids using furfural as a carbon source in media without glucose. The strain degraded furfural and produced other compounds while accumulating lipids. This finding provides a potential method for utilizing furfural as a renewable carbon source.
BIORESOURCE TECHNOLOGY
(2022)
Article
Microbiology
Linnea Qvirist, Friederike Mierke, Ricardo Vazquez Juarez, Thomas Andlid
Summary: This study screened naturally-occurring oleaginous yeasts for lipid production on xylose, glycerol, and natural plant hydrolysate. The strains Pseudozyma hubeiensis BOT-O and Rhodosporidium toruloides BOT-A2 were identified as having high lipid accumulation capabilities. These strains showed potential as production strains with high levels of storage lipids and similar growth rates on various substrates compared to currently used lipid production strains. The oils produced by these strains had fatty acid profiles similar to various plant oils, making them promising for future applications in fuels, food, or feed production.
Article
Green & Sustainable Science & Technology
Carlos S. Osorio-Gonz, Rahul Saini, Krishnamoorthy Hegde, Satinder Kaur Brar, Alain Lefebvre, Antonio Avalos Ramirez
Summary: The interest in microbial lipids has increased recently due to their extensive use in producing value-added compounds in various industries. Rhodosporidium toruloides is a suitable option for lipid production as it can consume five-carbon sugars, accumulate high levels of lipids, and tolerate toxic compounds. The study investigated the effect of different sugar ratios, carbon/nitrogen ratios, and the use of Na2HPO4 as an inducer on biomass production, sugar consumption, and lipid accumulation. The results showed that the optimal conditions led to a maximum lipid accumulation of 5.35 g/L.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Junting Wang, Xiaojia Guo, Li Wan, Yuxue Liu, Haizhao Xue, Zongbao K. Zhao
Summary: This study demonstrates that FalDH variants can be designed to prefer non-natural cofactor NCD by structure-guided modification of the binding pocket. The best variant, FalDH 9B2, showed over 150-fold higher preference for NCD compared to NAD. These engineered enzymes, along with other NCD-linked enzymes, provide opportunities for the biological conversion of C1 molecules.
Article
Chemistry, Multidisciplinary
Ding-Wei Ji, Yan-Cheng Hu, Xiang-Ting Min, Heng Liu, Wei-Song Zhang, Ying Li, Yongjin J. Zhou, Qing-An Chen
Summary: This study demonstrates a unique skeleton-reorganizing coupling reaction of cycloheptatriene and cycloalkenones with amines. The reaction selectively couples cycloheptatriene with anilines to deliver fused 1,2-dihydroquinoline products in the presence of Rh/acid catalysis. Mechanistic studies reveal that the ring reorganization occurs through retro-Mannich type ring-opening and subsequent intramolecular Povarov reaction. The synthetic utilization of this reaction is showcased with gram-scale reaction, synthetic derivatizations, and late-stage modification of commercial drugs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Chunxiao Yan, Ning Gao, Xuan Cao, Lun Yao, Yongjin J. Zhou, Jiaoqi Gao
Summary: This study found that the deficiency of amino acids and nucleotides has effects on cell growth and product synthesis in yeast. These effects can be restored by adding appropriate supplementation of amino acids or nucleotides.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Xuan Cao, Wei Yu, Yu Chen, Shan Yang, Zongbao K. Zhao, Jens Nielsen, Hongwei Luan, Yongjin J. Zhou
Summary: This study used a modular approach to globally rewire the cellular metabolism and achieved a sclareol production of 11.4 g/L, the highest reported titer in microbes. Metabolic flux analysis showed that modular balanced metabolism directed the metabolic flux towards the biosynthesis of targeted molecules, and transcriptomic analysis revealed that the expression of central metabolism genes was shaped for a new balanced metabolism, which laid a foundation for extensive metabolic engineering of other microbial species for sustainable bio-production.
METABOLIC ENGINEERING
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Yongjin Zhou, Martin Grininger, Hal Alper
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Min Ye, Jiaoqi Gao, Yongjin J. Zhou
Summary: Bioproduction of natural products using microbial cell factories is a promising alternative to traditional plant extraction. In this study, the cellular metabolism of Ogataea polymorpha was rewired to achieve high-level production of the sesquiterpenoid beta-elemene. By optimizing the mevalonate pathway, enhancing the supply of NADPH and acetylCoA, and downregulating competitive pathways, the engineered strain produced significant amounts of beta-elemene. This research highlights the potential industrial application of O. polymorpha as a microbial platform for producing sesquiterpenoids.
METABOLIC ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Xiaoyan Wu, Peng Cai, Linhui Gao, Yunxia Li, Lun Yao, Yongjin J. Zhou
Summary: Genetically modified industrial yeast Pichia pastoris was engineered to efficiently produce 3-hydroxypropionic acid (3-HP) from methanol. Through optimization of gene expression, substrate supply, and NADPH availability, 48.2 g/L of 3-HP was obtained in large-scale bioreactor cultivation. This study highlights the potential of P. pastoris for methanol biotransformation and provides strategies for the production of other acetyl-CoA derivatives from methanol.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Agriculture, Multidisciplinary
Chunyang Cao, Haiyan Zhang, Xuan Cao, Sijia Kong, Beiwei Zhu, Xinping Lin, Yongjin J. Zhou
Summary: Engineering microorganisms to synthesize isoprenoids is an efficient approach to increase their concentration. In this study, three types of isoprenoid pathways were constructed and optimized in yeast peroxisomes to produce the sesquiterpene (+)-valencene. The Haloarchaea-type MVA pathway was found to be more effective, and a production of 869 mg/L (+)-valencene was achieved. This work expands isoprenoid synthesis in eukaryotes and provides a more efficient pathway for isoprenoid production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jiaoqi Gao, Wei Yu, Yunxia Li, Mingjie Jin, Lun Yao, Yongjin J. Zhou
Summary: Engineered yeast Ogataea polymorpha has been used to co-utilize glucose and xylose for the production of free fatty acids (FFAs) and 3-hydroxypropionic acid (3-HP) from lignocellulose.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Shujing Qiao, Fan Bai, Peng Cai, Yongjin J. Zhou, Lun Yao
Summary: An improved CRISPRi system in Pichia pastoris has been developed by fusing dCas9 with endogenous transcriptional repressor domains. This system shows strong repression of eGFP gene, with the highest efficiency of 85%. The gRNA expression and processing play a critical role in CRISPRi efficiency, and Csy4 endoribonuclease shows higher repression efficiency.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Wei Yu, Jiaoqi Gao, Lun Yao, Yongjin J. Zhou
Summary: Metabolic engineering of industrial methylotrophic yeast Ogataea polymorpha was conducted to overproduce 3-hydroxypropionate (3-HP), an important platform chemical that can be used for the production of various special chemicals.
CHINESE JOURNAL OF CATALYSIS
(2023)
Article
Biotechnology & Applied Microbiology
Ning Gao, Jiaoqi Gao, Wei Yu, Sijia Kong, Yongjin J. J. Zhou
Summary: Dynamic coupling of biosynthetic pathways with cellular metabolism at a spatial-temporal manner can improve production and alleviate cellular stresses. In this study, spatial-temporal regulation strategy was used to partially coordinate fatty alcohol biosynthesis with cellular metabolism, resulting in improved production.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)
Article
Multidisciplinary Sciences
Peng Cai, Xiaoyan Wu, Jun Deng, Linhui Gao, Yiwei Shen, Lun Yao, Yongjin J. Zhou
Summary: Methanol-based biorefinery is a promising strategy for achieving carbon neutrality goals. This study successfully rewired the global metabolism of Pichia pastoris to efficiently produce free fatty acids and fatty alcohols from methanol.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Letter
Microbiology
Sijia Kong, Wei Yu, Ning Gao, Xiaoxin Zhai, Yongjin J. Zhou
Summary: In this study, we identified 30 neutral sites in the Saccharomyces cerevisiae genome that do not affect cellular fitness using the efficient CRISPR-Cas9 technique. These neutral sites can be used for extensive metabolic engineering and chemical production.
FEMS MICROBIOLOGY LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Yunxia Li, XiaoXin Zhai, Wei Yu, Dao Feng, Aamer Ali Shah, Jiaoqi Gao, Yongjin J. Zhou
Summary: This study demonstrated the capacity of O. polymorpha for overproduction of free fatty acids (FFAs) from various carbon sources, and revealed a synergistic utilization of xylose and methanol in the FFA producing strain.
BIORESOURCES AND BIOPROCESSING
(2022)