Restriction endonucleases: natural and directed evolution

Type II restriction endonuclease R.Hpy188I belongs to the GIY-YIG nuclease superfamily, but exhibits an unusual active site

(2012) Katarzyna H Kaminska et al.   BMC STRUCTURAL BIOLOGY

Degenerate sequence recognition by the monomeric restriction enzyme: single mutation converts BcnI into a strand-specific nicking endonuclease

(2011) Georgij Kostiuk et al.   NUCLEIC ACIDS RESEARCH

Generation of a Manganese Specific Restriction Endonuclease with Nicking Activity

(2010) Kommireddy Vasu et al.   BIOCHEMISTRY

TAL effectors: finding plant genes for disease and defense

(2010) Adam J Bogdanove et al.   CURRENT OPINION IN PLANT BIOLOGY

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

Directed Evolution of an Enhanced and Highly Efficient FokI Cleavage Domain for Zinc Finger Nucleases

(2010) Jing Guo et al.   JOURNAL OF MOLECULAR BIOLOGY

Creating Designed Zinc-Finger Nucleases with Minimal Cytotoxicity

(2010) Sivaprakash Ramalingam et al.   JOURNAL OF MOLECULAR BIOLOGY

Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures

(2010) Yannick Doyon et al.   NATURE METHODS

Sequence-specific cleavage of RNA by Type II restriction enzymes

(2010) Iain A. Murray et al.   NUCLEIC ACIDS RESEARCH

Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity

(2010) S.-H. Chan et al.   NUCLEIC ACIDS RESEARCH

Identification of new homologs of PD-(D/E)XK nucleases by support vector machines trained on data derived from profile-profile alignments

(2010) M. Laganeckas et al.   NUCLEIC ACIDS RESEARCH

Expanded Genetic Alphabets in the Polymerase Chain Reaction

(2009) Zunyi Yang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Homing endonucleases: from basics to therapeutic applications

(2009) Maria J. Marcaida et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

On the Divalent Metal Ion Dependence of DNA Cleavage by Restriction Endonucleases of the EcoRI Family

(2009) Vera Pingoud et al.   JOURNAL OF MOLECULAR BIOLOGY

Changing the DNA Recognition Specificity of the EcoDam DNA-(Adenine-N6)-Methyltransferase by Directed Evolution

(2009) Sanjay Chahar et al.   JOURNAL OF MOLECULAR BIOLOGY

Targeting individual subunits of the FokI restriction endonuclease to specific DNA strands

(2009) K. L. Sanders et al.   NUCLEIC ACIDS RESEARCH

Creation of a type IIS restriction endonuclease with a long recognition sequence

(2009) Shaun M. Lippow et al.   NUCLEIC ACIDS RESEARCH

Rational engineering of type II restriction endonuclease DNA binding and cleavage specificity

(2009) Richard D. Morgan et al.   NUCLEIC ACIDS RESEARCH

Differences between Ca 2+ and Mg 2+ in DNA binding and release by the SfiI restriction endonuclease: implications for DNA looping

(2009) Stuart R. W. Bellamy et al.   NUCLEIC ACIDS RESEARCH

Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs

(2009) Priscilla Hiu-Mei Too et al.   NUCLEIC ACIDS RESEARCH

Crystal structure of the ββα-Me type II restriction endonuclease Hpy99I with target DNA

(2009) Monika Sokolowska et al.   NUCLEIC ACIDS RESEARCH

The MmeI family: type II restriction–modification enzymes that employ single-strand modification for host protection

(2009) Richard D. Morgan et al.   NUCLEIC ACIDS RESEARCH

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

In Vivo DNA Protection by Relaxed-Specificity SinI DNA Methyltransferase Variants

(2008) E. Timar et al.   JOURNAL OF BACTERIOLOGY

Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases

(2008) Xiangdong Meng et al.   NATURE BIOTECHNOLOGY

Template-directed addition of nucleosides to DNA by the BfiI restriction enzyme

(2008) Giedrius Sasnauskas et al.   NUCLEIC ACIDS RESEARCH

Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleases

(2008) Heiko Kuhn et al.   NUCLEIC ACIDS RESEARCH

Structural and evolutionary classification of Type II restriction enzymes based on theoretical and experimental analyses

(2008) Jerzy Orlowski et al.   NUCLEIC ACIDS RESEARCH

Evolution of sequence specificity in a restriction endonuclease by a point mutation

(2008) M. Saravanan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell death upon epigenetic genome methylation: a novel function of methyl-specific deoxyribonucleases

(2008) Eri Fukuda et al.   GENOME BIOLOGY

Discovery and distribution of super-integrons among Pseudomonads

(2003) Romualdas Vaisvila et al.   MOLECULAR MICROBIOLOGY