Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 89, Issue 1, Pages 91-98Publisher
SPRINGER
DOI: 10.1007/s00253-010-2831-9
Keywords
Aliphatic nitrilase; Dinitrile; Metagenome; Regioselectivity
Categories
Funding
- BMBF Innovationsinitiative Neue Lander (Unternehmen Region, InnoProfile)
Ask authors/readers for more resources
Several novel nitrilases were selected from meta-genomic libraries using cinnamonitrile and a mixture of six different nitriles as substrates. The nitrilase gene nit1 was expressed in Escherichia coli and the resulting protein was further examined concerning its biochemical properties. Nit1 turned out to be an aliphatic nitrilase favoring dinitriles over mononitriles. Stereochemical analysis revealed that Nit1 converted the dinitrile 2-methylglutaronitrile regioselectively. Hydrolysis at the omega-nitrile group of a dinitrile, such as catalyzed by Nit1, leads to omega-cyanocarboxylic acids, which are important precursors for chemical and pharmaceutical products. Nit1 metabolized 2-methylglutaronitrile to the corresponding omega-cyanocarboxylic acid 4-cyanopentanoic acid can be used for the production of the fine chemical 1,5-dimethyl-2-piperidone.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available