Article
Biochemistry & Molecular Biology
Minjie Gao, Guoshuai Yang, Feifei Li, Zichao Wang, Xiuyu Hu, Yun Jiang, Jiajun Yan, Zhitao Li, Xiaobei Zhan
Summary: This study involved the production of curdlan oligosaccharides by expressing endo-11-1,3-glucanase in Pichia pastoris and constructing a co-culture system. The optimized conditions in a 7-L bioreactor led to a maximum yield of 18.77 g/L of curdlan oligosaccharides with 3-10 degrees of polymerization, presenting a novel strategy for oligosaccharide production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Min-Jie Gao, Jia-Jun Yan, Yue Zhao, Li Zhu, Guo-Shuai Yang, Xiao-Bei Zhan
Summary: In this study, the endo-beta-1,3-glucanase gene from Trichoderma harzianum was successfully expressed in Pichia pastoris KM71, resulting in a high-yield endo-beta-1,3-glucanase strain. The recombinant enzyme exhibited optimal activity at pH 5.5 and 50 degrees C, with efficient hydrolysis capabilities towards scleroglucan and schizophyllan.
PROCESS BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Wenyuan Zhou, Sheng Tong, Farrukh Raza Amin, Wuxi Chen, Jinling Cai, Demao Li
Summary: In this study, endoglucanase (MtEG5-1) from Myceliophthora thermophila was successfully overexpressed in Pichia pastoris GS115. The purified MtEG5-1 showed optimal activity at pH 5 and 70 degrees C, with good thermal stability. The enzyme demonstrated a molecular weight of 55 kDa and had Km and Vmax values of approximately 6.11 mg/mL and 91.74 mu mol/min/mg at 70 degrees C (pH 5.0), respectively. The activity of MtEG5-1 was enhanced by Mn2+ and inhibited by K+. These findings suggest that MtEG5-1 expressed by P. pastoris GS115 is more heat-tolerant and suitable for future academic research and industrial applications.
FERMENTATION-BASEL
(2023)
Article
Green & Sustainable Science & Technology
Minjie Gao, Feiyang Duan, Liping Liu, Xiuyu Hu, Li Zhu, Yun Jiang, Xiaobei Zhan
Summary: Utilizing waste liquid containing miscellaneous waste carbohydrates (MWC) from the high-fructose syrup (HFS) production industry in China as an alternative carbon source for Pichia pastoris fermentation, the study successfully optimized the fermentation processes and achieved high production of endo-beta-1,3-glucanase. This cleaner production approach significantly reduced environmental pollution and production costs in both HFS and P. pastoris fermentation industries.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Agricultural Engineering
Minjie Gao, Ying Xu, Guoshuai Yang, Shuxia Jin, Xiuyu Hu, Yun Jiang, Li Zhu, Zhitao Li, Xiaobei Zhan
Summary: The successful implementation of heterologous expression with error-prone PCR technology in Pichia pastoris led to the mutant strain 7 N12 with efficient specific activities for degrading curdlan and scleroglucan. Coupled fermentation of Pichia pastoris and Sclerotium rolfsii simplified the enzymatic 8-oligoglucoside preparation procedures, showing potential for industrial production of functional oligosaccharides.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Minjie Gao, Yuchen Zhou, Jiajun Yan, Li Zhu, Zhitao Li, Xiuyu Hu, Xiaobei Zhan
Summary: Rhodium is widely used in catalysis, but its adsorption by microorganisms is not well studied. In this work, waste P. pastoris and recombinant P. pastoris GS-R were used as novel adsorbents for Rh(III) adsorption, showing high adsorption capacity and selectivity. The study developed an environmentally-friendly strategy for recovering Rh(III) and other precious metals using P. pastoris.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Chao Liu, Jin-Song Gong, Chang Su, Hui Li, Heng Li, Zhi-Ming Rao, Zheng-Hong Xu, Jin-Song Shi
Summary: Pichia pastoris has been recognized as an important platform for heterologous protein production. This article summarizes the process of protein secretion, modification, and transportation in P. pastoris, as well as the roles played by key proteins and their co-expression effects.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lukas Rieder, Katharina Ebner, Anton Glieder, Morten Sorlie
Summary: The study established a method to express fungal LPMOs using a Pichia pastoris platform, achieving high yields and easy purification. It is the first report that a yeast-derived signal peptide can successfully process LPMOs, forming fully functional enzymes.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biochemistry & Molecular Biology
Zhengxue Li, Chengtuo Niu, Xiaohong Yang, Feiyun Zheng, Chunfeng Liu, Jinjing Wang, Qi Li
Summary: In this study, a mesophilic beta-glucanase from Bacillus terquilensis CGX 5-1 was engineered to improve its stability at acidic environment and catalytic property. The Q1E/I133L variant showed better acidic stability and higher catalytic activity, with potential applications in industries. The improved acidic resistance ability of the variant may be attributed to more surface negative charge, decreased exposure degree of residue No.1, shifted side chain direction of residue No.133, and lower total and folding free energy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Environmental Sciences
Zi-Qian Zha, Shuai You, Yang-Hao Hu, Fang Zhang, Yi-Wen Chen, Jun Wang
Summary: N-glycosylation can alter the properties of enzymes, such as increasing thermostability and catalytic efficiency. This study focused on the modification and utilization of GH16 family glucanases, showing that N-glycosylation can enhance their stability and efficiency.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Xiaoyan Wu, Peng Cai, Linhui Gao, Yunxia Li, Lun Yao, Yongjin J. Zhou
Summary: Genetically modified industrial yeast Pichia pastoris was engineered to efficiently produce 3-hydroxypropionic acid (3-HP) from methanol. Through optimization of gene expression, substrate supply, and NADPH availability, 48.2 g/L of 3-HP was obtained in large-scale bioreactor cultivation. This study highlights the potential of P. pastoris for methanol biotransformation and provides strategies for the production of other acetyl-CoA derivatives from methanol.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Dunchi Xiao, Xun Li, Yu Zhang, Fei Wang
Summary: In this study, different promoters and signal peptides were compared for expressing codon-optimized CALB in Pichia pastoris. Recombinant P. pastoris containing 3 copies of calb gene were obtained by screening with high concentrations of antibiotics. The maximum CALB activity and total protein content reached 444.46 +/- 18.81 U/mL and 5.41 +/- 0.1 g/L, respectively, after about 9 days of incubation in FM22 medium.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Wenxin Zhang, Yixin Zhang, Yu Lu, Richard Ansah Herman, Sheng Zhang, Yanghao Hu, Weiguo Zhao, Jun Wang, Shuai You
Summary: This study improved the thermostability of a glucanase enzyme by conducting structure-based surface charge engineering, leading to increased catalytic activity and reduced viscosity. This has significant implications for the brewing industry.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Alexander Lisov, Oksana Belova, Zoya Lisova, Alexey Nagel, Andrey Shadrin, Zhanna Andreeva-Kovalevskaya, Maxim Nagornykh, Marina Zakharova, Alexey Leontievsky
Summary: Two potential endoglucanases encoded by genes Cfla_2912 and Cfla_2913 were identified in the genome of Cellulomonas flavigena. These genes were cloned and expressed in Pichia pastoris, resulting in the production of two proteins with beta-glucanase activity. The enzymes exhibited high activity against barley beta-glucan and lichenan, weak activity against carboxymethyl cellulose (CMC) and phosphoric-acid treated cellulose, and no activity against other substrates tested. Both proteins showed a neutral pH optimum of about 7.0 and were stable at neutral to slightly alkaline pH. They efficiently hydrolyzed cereal polysaccharides, indicating their potential for biotechnological applications.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sheng-Ling Chen, Ting-Shan Liu, Wei-Guo Zhang, Jian-Zhong Xu
Summary: By re-creating the NADPH and ATP biosynthetic pathways in Pichia pastoris, the production of alpha-farnesene was increased. Overexpressing ZWF1 and SOL3 improved NADPH supply, while inactivating PGI did not. Low intensity expression of cPOS5 aided in the production of alpha-farnesene. Finally, ATP production was increased by overexpressing APRT and inactivating GPD1. The resultant strain produced 41.7% more alpha-farnesene than the parent strain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xiao Lan Liu, Narasimha Kumar Kopparapu, Hong Chen Zheng, Priti Katrolia, Yong Ping Deng, Xi Qun Zheng
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2016)
Article
Biochemistry & Molecular Biology
Yongping Deng, Xiaolan Liu, Priti Katrolia, Narasimha Kumar Kopparapu, Xiqun Zheng
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2018)
Article
Biochemistry & Molecular Biology
Peng Zhou, Huifang Zhu, Qiaojuan Yan, Priti Katrolia, Zhengqiang Jiang
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2011)
Article
Agricultural Engineering
Priti Katrolia, Huiyong Jia, Qiaojuan Yan, Shuang Song, Zhengqiang Jiang, Haibo Xu
BIORESOURCE TECHNOLOGY
(2012)
Article
Biotechnology & Applied Microbiology
Guangsen Fan, Priti Katrolia, Huiyong Jia, Shaoqing Yang, Qiaojuan Yan, Zhengqiang Jiang
BIOTECHNOLOGY LETTERS
(2012)
Article
Chemistry, Applied
Priti Katrolia, Peng Zhou, Pan Zhang, Qiaojuan Yan, Yinan Li, Zhengqiang Jiang, Haibo Xu
CARBOHYDRATE POLYMERS
(2012)
Review
Biotechnology & Applied Microbiology
Priti Katrolia, Eranna Rajashekhara, Qiaojuan Yan, Zhengqiang Jiang
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2014)
Article
Chemistry, Applied
Priti Katrolia, Min Zhang, Qiaojuan Yan, Zhengqiang Jiang, Chunlei Song, Lite Li
Article
Agriculture, Multidisciplinary
Priti Katrolia, Qiaojuan Yan, Pan Zhang, Peng Zhou, Shaoqing Yang, Zhengqiang Jiang
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Priti Katrolia, Qiaojuan Yan, Huiyong Jia, Yinan Li, Zhengqiang Jiang, Chunlei Song
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2011)
Article
Biochemistry & Molecular Biology
Priti Katrolia, Xiaolan Liu, Guanlong Li, Narasimha Kumar Kopparapu
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Priti Katrolia, Xiaolan Liu, Junzhong Li, Narasimha Kumar Kopparapu
JOURNAL OF FOOD BIOCHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Priti Katrolia, Xiaolan Liu, Yiyu Zhao, Narasimha Kumar Kopparapu, Xiqun Zheng
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Review
Food Science & Technology
Narasimha Kumar Kopparapu, Yajie Duan, Linhua Huang, Priti Katrolia
Summary: Food wastage is a pressing global issue that needs immediate attention. Utilizing by-products and converting them into value-added products can contribute to a sustainable economy. This review focuses on the modification of corn gluten meal (CGM) and its potential applications in food and other industries.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Yajie Duan, Priti Katrolia, Ailing Zhong, Narasimha Kumar Kopparapu
Summary: A novel thrombolytic enzyme was produced using a food grade microorganism, Neurospora crassa, and agro-industrial by-products as substrates. The enzyme exhibited high fibrinolytic and anticoagulant activities, making it a potential candidate for thrombolytic agent development.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2022)